Cell-free Protein Synthesis- PURExpress™ In vitro Protein Synthesis Kit


Delta-Ribosome Kit also available now!



The PURExpress™ In Vitro Protein Synthesis Kit is a novel cell-free transcription/translation system reconstituted from purified components necessary for E. coli translation. All of the activities have been expressed from recombinant sources, enabling PURExpress to be free of contaminating exonucleases, RNases and proteases. This prevents degradation of template DNA and results in proteins that are free of modification and degradation. Transcription and translation are carried out in a one-step reaction, and require the mixing of only two tubes.
With results available in only a few hours, PURExpress saves valuable laboratory time and is ideal for high throughput technologies.


Advantages:

  • Cleaner system – lack of endogenous proteases or nucleases eliminates sample degredation
  • Flexible – can be used for circular or linear template
  • Simple analysis – synthesized protein can often be visualized on a Coomassie stained gel
  • Easy-to-use – requires the mixing of two tubes followed by the addition of template DNA
  • Fast – reaction is complete in approximately one hour
  • Efficient – purified components enable more control of translational, secretory and folding machinery

Expression and reverse purification of DHFR (A) and T4 DNA Ligase (B) using PURExpress. 125 μl reactions were carried out according to recommendations in accompanying manual. Samples were analyzed on a 10–20% Tris-glycine gel and stained with Coomassie Blue. Note that in both cases, the desired protein can be visualized in the total protein fraction. The red dot indicates the protein of interest. Marker M: Protein Ladder #P7703



 NEB#
  Product  Size  Price
 E6800 S  PURExpress™ In vitro Protein Synthesis Kit 10 Reactions (25 μl each) 212,- Euro
 E3313 S  PURExpress™ Δ Ribosome Kit 10 Reactions (25 μl each) 295,- Euro

 Complementary product: 
 M0314 S  Murine RNase Inhibitor 3.000 units 66,- Euro
 M0314 L  Murine RNase Inhibitor 15.000 units 264,- Euro


Licenses/Patents/Disclaimers: PURExpress™ is based on the PURE System Technology originally developed by Dr. Takuya Ueda at the University of Tokyo and commercialized as the PURESYSTEM® by BioComber (Tokyo, Japan).

Licensed from BioComber (Tokyo, Japan) under Patent Nos. 7,118,883; WO2005-105994 and JP2006-340694. For research use only. Commercial use of PURExpress™ Δ Ribosome Kit requires a license from New England Biolabs, Inc.