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Phusion® High-Fidelity DNA Polymerase  

- Extreme Performance 

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Phusion® High-Fidelity DNA Polymerase offers performance that no other enzyme can match. Getting the most out of your amplification reactions has always been difficult. Optimizing fidelity, specificity, and yield has been challenging with a single enzyme. Phusion DNA Polymerase combines the fidelity you demand with unparalleled speed and robustness.


Phusion DNA Polymerase brings together a novel Pyrococcus-like enzyme with a processivity-enhancing domain and generates PCR products with accuracy and speed previously unattainable with a single enzyme, even on your most difficult templates. Additionally, Phusion DNA Polymerase is capable of amplifying long templates. In the quality control tests of Phusion DNA Polymerase, 7.5 kb genomic DNA and 20 kb λ DNA are routinely amplified. The unique structure and characteristics of Phusion DNA Polymerase make it a superior choice for cloning. The Phusion High-Fidelity DNA Polymerase sets a new standard for PCR performance.


 

Phusion® Hot Start Flex DNA Polymerase delivers additional flexibility to your reaction setup and cycling conditions. In contrast to chemically modified or antibody-based hot start polymerases, Phusion Hot Start Flex utilizes an innovative synthetic aptamer. This structure binds to the polymerase through non-covalent interactions, blocking activity during reaction setup. The polymerase is activated during normal cycling conditions, allowing reactions to be set up at room temperature. 

Phusion® Hot Start Flex DNA Polymerases do not require a separate high temperature incubation step, shortening reaction times and increasing ease-of-use. Phusion Hot start Flex is an ideal choice for high specificity amplification over a flexible range of annealing temperatures and offers robust amplification on a wide variety of templates.



Advantages:
  • Extreme Fidelity - Highest of any available thermostable polymerase (50X greater than Taq)
     
  • High Speed - Extension times are dramatically reduced (10X faster than Pfu)
     
  • Robust Reactions - Maximal success with minimal optimization
     
  • High Yield - Increased product yield with minimal enzyme amount  
  • Now Hot Start Polymerase available!
  
Fig.: 3.8 kb fragment was amplified from 50 ng of Jurkat gDNA using different polymerases. Reactions were carried out according to the manufacter's recommended conditions (cycling conditions are shown below). Extension times are indicated (in minutes).. Ladder L is a 1 kb DNA Ladder #N3232.


 
Art#  Description amount list price
M0530 S  Phusion® High-Fidelity DNA Polymerase 

 

 100 units 90,- €
M0530 L  Phusion® High-Fidelity DNA Polymerase 500 units 399,- €
M0531 S  Phusion® High-Fidelity HF PCR Master Mix 100 rxn 150,- €
M0531 L  Phusion® High-Fidelity HF PCR Master Mix 500 rxn 600,- €
M0532 S  Phusion® High-Fidelity GC PCR Master Mix 100 rxn 150,- €
M0532 L  Phusion® High-Fidelity GC PCR Master Mix 500 rxn 600,- €
E0553 S  Phusion® High-Fidelity PCR Kit 50 units 60,- €
E0553 L  Phusion® High-Fidelity PCR Kit 200 units 210,- €
 New: Phusion® Hot Start Flex Polymerases
M0535 S  Phusion® Hot Start Flex DNA Polymerase 100 units 105,- €
M0535 L

 Phusion® Hot Start Flex DNA Polymerase

 500 units 480,- €
M0536 S

 Phusion® Hot Start Flex 2X Master Mix

 100 rxn
(50 µl volume)		 175,- €
M0536 L

 Phusion® Hot Start Flex 2X Master Mix

 500 rxn
(50 µl volume)		 700,- €
 complementary products:
N0447 S  Deoxynucleotide Solutions, Mix 8 µmol each 58,- €
N0447 L  Deoxynucleotide Solutions, Mix 40 µmol each 232,- €

 

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