OneTaq^TM DNA Polymerase and
OneTaq^TM Hot Start DNA Polymerase

– the ONE you've been waiting for!

An optimized blend of Taq and Deep Vent_R^® DNA polymerases, OneTaq^TM and OneTaq^TM Hot Start DNA Polymerases offer robust amplification across a wide range of templates. The 3 ́–5 ́ exonuclease activity of Deep Vent_R DNA Polymerase increases the fidelity and robustness of Taq, and the hot start formulation combines convenience with decreased interference from primer-dimers and secondary products. Additionally, OneTaq reaction buffers and High GC Enhancer have been formulated for robust yields with minimal optimization, regardless of a template’s GC content.

OneTaq DNA Polymerase is supplied with two 5X buffers (Standard and GC), as well as a High GC Enhancer solution. For most routine and/or AT- rich amplicons or complex amplicons with up to ~65% GC content, OneTaq Standard Reaction Buffer provides robust amplification. For GC-rich amplicons, the OneTaq GC Reaction Buffer can improve both performance and yield. For particularly high GC (>65%) or difficult amplicons, the OneTaq High GC enhancer can be added to reactions containing OneTaq GC Buffer. These formulations ensure maximum performance for routine, AT- or GC-rich amplicons.

• Exceptional performance in endpoint PCR across a wide range of templates

• Robust yields with minimal optimization

• Convenient product formats (stand-alone enzyme, master mixes, and Quick-Load® formats)

• 2x higher fidelity (“proof-reading”) compared to standard Taq DNA Polymerase

• d(A) overhangs at the 3 ́end of PCR product enable ligation to d(T)/d(U)- overhang vectors

• Compatible with standard Taq protocols

Comparison of OneTaq DNA Polymerase to other commercially available polymerases (non-hot start):
Amplifikation einer Auswahl an GC-reicher humaner gDNA verdeutlicht die Überlegenheit der OneTaq DNA Polymerase (braun). A.) Reinheit des Produktes und B.) Ausbeuten der Reaktion wurden nach PCR mit Hilfe einer Microfluidic Analysis ermittelt (Mittelwert aus  dreiunabhängigen Ansätzen) und grafisch dargestellt. Die PCRs erfolgten nach Empfehlungen des Herstellers (30 Zyklen).