OneTaq Quick-Load DNA Polymerase: After PCR directly onto the gel. Test now!
OneTaq DNA Polymerase is an optimized blend of Taq and Deep Vent DNA polymerases for use with standard and difficult PCR experiments. The 3´→ 5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase. The OneTaq Reaction Buffers and High GC Enhancer have been formulated for robust yields with minimal optimization, regardless of a template’s GC content.
OneTaq DNA Polymerase is supplied with two 5X buffers (Standard and GC), as well as a High GC Enhancer solution. For most standard and/or AT-rich amplicons (Lambda, etc.) or complex amplicons with up to ~65% GC content, OneTaq Standard Reaction Buffer provides robust amplification. For GC-rich amplicons, the OneTaq GC Reaction Buffer can improve both performance and yield. For particularly high GC or difficult amplicons, the OneTaq High GC Enhancer can be added at a final concentration of 10–20% to reactions containing OneTaq GC Reaction Buffer.
For PCR on „Standard“ DNA templates (up to 6 kb and ca. 55% GC content) we recommend the new OneTaq Quick-Load DNA Polymerase. Besides the conventional reaction buffer this new variant of the OneTaq DNA Polymerase contains a colored Quick-Load reaction buffer for direct gel loading on agarose gels after PCR.
OneTaq Quick-Load DNA Polymerase is therefore particularly well suited for PCR like genotyping, colony PCR etc.
Quick – reliable – favorable!
Click here for further information on OneTaq DNA Polymerase:
Amplification of a variety of DNA targets (human and viral templates) demonstrates strong performance of the OneTaq Quick-Load DNA Polymerase. Product purity (bars, left axis) and yield (circles, right axis) were calculated via microfluidic analysis from triplicate reactions after 30 cycles of PCR. Standard deviation is indicated by error bars (yield) or shaded bands (purity). GoTaq was cycled according to manufacturer’s recommendations.
|OneTaq® Quick-Load® DNA Polymerase||M0509 S||100 units||20 €|
|OneTaq® Quick-Load® DNA Polymerase||M0509 L||500 units||80 €|
|OneTaq® Quick-Load® DNA Polymerase||M0509 X||2500 units||375 €|
|Deoxynucleotide (dNTP) Solution Mix||N0447 S/L||8 µmol/40 µmol||58 € / 232 €|
|Deoxynucleotide (dNTP) Solution Set||N0446 S||25 µmol||158 €|
You would like to test our OneTaq Quick-Load free of charge beforehand?
Then request a free sample – but hurry up because the number of samples is limited*.
*Limited to 1 sample per working group, as long as stock lasts.