OneTaq Quick-Load

OneTaq Quick-Load DNA Polymerase

OneTaq Quick-Load DNA Polymerase: After PCR directly onto the gel. Test now!

OneTaq DNA Polymerase is an optimized blend of Taq and Deep Vent DNA polymerases for use with standard and difficult PCR experiments. The 3´→ 5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase. The OneTaq Reaction Buffers and High GC Enhancer have been formulated for robust yields with minimal optimization, regardless of a template’s GC content.

OneTaq DNA Polymerase is supplied with two 5X buffers (Standard and GC), as well as a High GC Enhancer solution. For most standard and/or AT-rich amplicons (Lambda, etc.) or complex amplicons with up to ~65% GC content, OneTaq Standard Reaction Buffer provides robust amplification. For GC-rich amplicons, the OneTaq GC Reaction Buffer can improve both performance and yield. For particularly high GC or difficult amplicons, the OneTaq High GC Enhancer can be added at a final concentration of 10–20% to reactions containing OneTaq GC Reaction Buffer.

For PCR on „Standard“ DNA templates (up to 6 kb and ca. 55% GC content) we recommend the new OneTaq Quick-Load DNA Polymerase. Besides the conventional reaction buffer this new variant of the OneTaq DNA Polymerase contains a colored Quick-Load reaction buffer for direct gel loading on agarose gels after PCR.

OneTaq Quick-Load DNA Polymerase is therefore particularly well suited for PCR like genotyping, colony PCR etc.
Quick – reliable – favorable!

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Amplification of a selection of sequences with varying GC content from human and C. elegans genomic DNA using OneTaq. Amplicon sizes are indicated next to gel, and GC content is indicated above gel. Marker M is the 1 kb DNA Ladder (NEB #N3232 ).

OneTaq Quick-Load Comparison

Amplification of a variety of DNA targets (human and viral templates) demonstrates strong performance of the OneTaq Quick-Load DNA Polymerase. Product purity (bars, left axis) and yield (circles, right axis) were calculated via microfluidic analysis from triplicate reactions after 30 cycles of PCR. Standard deviation is indicated by error bars (yield) or shaded bands (purity). GoTaq was cycled according to manufacturer’s recommendations.

Product list

OneTaq® Quick-Load® DNA Polymerase M0509 S 100 units 20 € NEB Shop_icon
OneTaq® Quick-Load® DNA Polymerase M0509 L 500 units 80 € NEB Shop_icon
OneTaq® Quick-Load® DNA Polymerase M0509 X 2500 units 375 € NEB Shop_icon

Complementary products

Deoxynucleotide (dNTP) Solution Mix N0447 S/L 8 µmol/40 µmol 58 € / 232 € NEB Shop_icon
Deoxynucleotide (dNTP) Solution Set N0446 S 25 µmol 158 € NEB Shop_icon

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