BioLux^TM Gaussia and Cypridina Luciferase Kits

NEB offers several products that utilize secreted Gaussia Luciferase (GLuc) and Cypridina Luciferase (CLuc) in reporter assays. These systems offer extreme sensitivity and can be used alone or together as a dual system.

Gaussia Luciferase (GLuc) is a 20 kDa luciferase from the marine cocepod Gaussia princeps. This luciferase, which does not require ATP, catalyzes the oxidation of the substrate coelenterazine in a reaction that produces light (480 nm), and has considerable advantages over other luminescent reporter genes. It is normally secreted from the cells and its secretion signal also functions very efficiently in mammalian cells. GLuc offers the advantage of a greatly increased bioluminescent signal relative to the commonly used firefly (Fluc) and Renilla luciferases (RLuc). GLuc was determined to emit light with a specific activity of 4.2 x 1024 photons/s/mol, the highest reported activity for any characterized luciferase (1).

Cypridina Luciferase (CLuc) is isolated from the marine ostracod Cypridina noctiluca and is efficiently secreted from mammalian cells (2,3). CLuc does not react with coelenterazine, a common substrate of marine luciferases. It uses a specific luciferin, which is not a substrate for Gaussia Luciferase. This allows the detection of both of GLuc and CLuc expressed from the same cells.

1.) Verhaegan, M.and Christopoulos, T.K. (2002) Anal. Chem. 74, 4378-4385.
2.) Wu, C. Chie, Suzuki-Ogoh, C., Ohmlya, Y. (2007) Biotech. 42, 290-292.
3.) Nakajima, et al. (2004) Biosci. Biotechnol. Biochem. 68, 565–570.

Advantages

• Naturally Secreted – Amenable to live cell assays


• Sensitivity – Brightest luciferases available; enables single cell applications


• Stability – Samples can be stored for several days with no loss of activity


• Easy-to-use – Cell lysis not necessary


• Non-destructive – Living cells can be used in downstream assays


• Flexible – Activity can also be measured in cell lysates

More Information:

DNA Sequences and Maps

FAQs for BioLuxTM Gaussia Luciferase Assay Kit

Protocols for BioLuxTM Gaussia Luciferase Assay Kit

FAQs for BioLuxTM Gaussia LumiFlex GLuc Assay Kit

Protocols for BioLuxTM Gaussia LumiFlex GLuc Assay Kit

BioLux™ Luciferase Starter Kits

Also availble: Luciferase Assay Buffer incl. Substrate

Also available: Luciferase Vectors