Cloning & Synthetic Biology
Tools
- NEBCloner
- NEBuilder
- NEB Golden Gate Assembly Tool
- Double Digest Finder
- NEB Cutter
- DNA Sequences and Maps Tool
- Enzyme Finder
Selection Tools
- Synthetic Biology/DNA Assembly Selection Chart
- DNA Ligase Selection Chart
- Isoschizomere der Restriktionsenzyme
Troubleshooting Guides
- Troubleshooting Guide for Cloning
- Restriction Enzyme Troubleshooting Guide
- Troubleshooting Guide for Ligases
- Troubleshooting Guide for End Modification
Hinweise und Tipps
- Restriction Enzyme Tips
- Optimizing Restriction Endonuclease Reactions
- Restriction enzymes requiring multi-sites for efficient cleavage
- NEBuffer Activity/Performance Chart with Restriction Enzymes
- Activity of Restriction Enzymes in a Q5®, Taq or Phusion PCR Mix
- Cloning Plasmids and DNAs Overview
- Compatible Cohesive Ends and Generation of New Restriction Sites
- Dam-Dcm and CpG Methylation
- Frequencies of Restriction Sites
- Recleavable Blunt Ends
- Recleavable Filled-in 5′ Overhangs
- Cleavage of Supercoiled DNA
- Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures
- Digestion of Agarose-Embedded DNA: Info for Specific Enzymes
- Restriction Endonucleases – Survival in a Reaction
- Traditional Cloning Quick Guide
- Tips for Maximizing Ligation Efficiencies
- End-Modification Tips
- Cleavage Close to the End of DNA Fragments
- Effect of various temperatures on Nicking Endonucleases
- Application Notes
- Improved methods for site-directed mutagenesis using Gibson Assembly® Master Mix
- Joining of Difficult to Ligate dsDNA Fragments with Blunt/TA Ligase Master Mix
Video Tutorials
RNA Biology
Hinweise und Tipps
- RNA Polymerase Selection Chart
- RNA Ligase Selection Chart
- cDNA Synthesis Selection Chart
- Recommended HiScribe RNA Synthesis Kits by Application
Guides
Video Tutorials
Glycobiology
Tools
Hinweise und Tipps
FAQs
Application Notes
- α1-3,6 Galactosidase Application Note
- Enzymatic removal of N- and O-glycans using PNGase F or the Protein Deglycosylation Mix P0704
- O-Glycosidase Application Note 1
Video Tutorials
Epigenetics
Troubleshooting Guides
Application Notes
Video Tutorials
DNA / RNA Ladders
Selection Tools
PCR DNA Amplification
Tools
Selection Tools
Troubleshooting Guides
Hinweise und Tipps
- Guidelines for PCR Optimization with Taq DNA Polymerase
- Guidelines for PCR Optimization with OneTaq® and OneTaq® Hot Start DNA Polymerases
- Guidelines for PCR Optimization with Thermophilic DNA Polymerases
FAQs
Application Notes
Video Tutorials
Next Generation Sequencing
Tools
Selection Tools
Hinweise und Tipps
Application Notes
- Low input ChIPSeq using the NEBNext Ultra Library Prep Kit for Illumina E7370
- Automated size selection of NEBNext Small RNA libraries with the Sage Pippin Prep
- Automated Library Preparation using NEBNext Ultra DNA Library
Prep Kit for Illumina on the HAMILTON STAR Line
Competent Cells
Selection Tools
- Characteristics of Select E.coli Strains
- Competent Cell Selection Guide
- Competitor Cross-Reference Tool
Troubleshooting Guides
Hinweise und Tipps
- Genetic Markers
- Making your own electrocompetent cells
- Making Unmethylated (Dam- Dcm-) DNA
- Restriction of Foreign DNA by E. coli K-12
- Additional E. coli Strain Genotypes
- McrA, McrBC and EcoKI Strain Phenotypes
Video Tutorials
Proteinexpression
Cellular analysis (SNAP-tag)
Selection Tools
Troubleshooting Guides
FAQs
Application Notes
- Labeling of Escherichia coli Expressed SNAP-tag® Fusion Proteins
- Labeling and Imaging of Cell Surface Receptors Mediated by SNAP-tag®
- Simultaneous Labeling of Two Proteins in Live Cells
- SNAPf based pulse labeling for analysis of protein turnover in living cells
Video Tutorials
Further information can be found in our Technical Resources section or at neb.com. Information on trademarks can be found here.
