NEB offers all you need for your RNA related workflows

In recent years, the discovery of new classes and modifications of RNA has ushered in a renaissance of RNA-focused research.

Did you know that NEB offers a broad portfolio of reagents for the purification, quantitation, detection, synthesis and manipulation of RNA? Experience improved performance and increased yields, enabled by our expertise in enzymology.

Utilize NEB’s RNA Toolbox for your RNA related experiments and research and benefit from excellent quality and performance.

Read the Feature Article:
Minding your caps and tails – considerations for functional mRNA synthesis

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Request your personal free copy of our new “RNA Technical Guide”.

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Protect your valuable RNA samples from digestion:
NEB’s murine RNase Inhibitor!

The recombinant, murine RNase Inhibitor from NEB is the best protection against omnipresent RNases (RNases A, B, C). Thanks to a point mutation compared to conventional (humane/porcine) RNase Inhibitors NEBs variant is longer active in experiments – even with lower DTT concentrations!

Further product information

Price comparison (Costs/100 units) based on published list prices as of 01/2018.

PRODUCT Prod. # SIZE PRICE
Murine RNase Inhibitor M0314S 3.000 units 71 € In den Shop wechseln Produktinformationen
Murine RNase Inhibitor M0314L 15.000 units 284 € In den Shop wechseln Produktinformationen

RNA Synthesis with high yields:
HiScribe RNA Synthesis Kits

In vitro RNA synthesis requires a DNA template, RNA polymerase, NTPs and other factors. High-yield robust reactions require optimization of each reaction component. NEB offers five in vitro RNA synthesis kits, all of which have been optimized to generate reproducible yields of quality RNA.

Further product information
PRODUCT Prod. # SIZE PRICE
HiScribe T7 High Yield RNA Synthesis Kit E2040S 50 rxns 223 € In den Shop wechseln Produktinformationen
HiScribe T7 Quick High Yield RNA Synthesis Kit E2050S 50 rxns 273 € In den Shop wechseln Produktinformationen
HiScribe T7 ARCA mRNA Kit (with Tailing) E2060S 20 rxns 376 € In den Shop wechseln Produktinformationen
HiScribe T7 ARCA mRNA Kit E2065S 20 rxns 322 € In den Shop wechseln Produktinformationen
HiScribe SP6 RNA Synthesis Kit E2070S 50 rxns 273 € In den Shop wechseln Produktinformationen

RNA preparation made easy:
Monarch® Total RNA Miniprep Kit

The “universal” Monarch Total RNA Purification Kit for quick and easy purification of total RNA from cells, tissue, blood, bacteria, plants…

Further product information
PRODUCT Prod. # SIZE PRICE
Monarch® Total RNA Miniprep Kit T2010S 50 preps 248 € In den Shop wechseln Produktinformationen
Monarch RNA Cleanup Kit (10 µg) T2030S 10 preps 52 €
In den Shop wechseln Produktinformationen
T2030L 100 preps 284 €
Monarch RNA Cleanup Kit (50 µg) T2040S 10 preps 50 € In den Shop wechseln Produktinformationen
T2040L 100 preps 280 €
Monarch RNA Cleanup Kit (500 µg) T2050S 10 preps 58 € In den Shop wechseln Produktinformationen
T2050L 100 preps 440 €

For precise and reliable RNA quantitation:
Luna® Universal qPCR & RT-qPCR Reagents

Fluorescence-based quantitative real-time PCR (qPCR) is the gold standard for the detection and quantification of nucleic acids due to its sensitivity and specificity. Luna products from NEB are optimized for qPCR or RT-qPCR, and are available for either intercalating dye or probe-based detection methods. All Luna products provide robust performance on diverse sample sources and target types.

Further product information
LUNA qPCR

RT-qPCR targeting human GAPDH was performed using the Luna Universal One-Step RT-qPCR Kit, featuring Luna WarmStart Reverse Transcriptase, …read more

PRODUCT Prod. # SIZE PRICE
Luna® Universal qPCR Master Mix M3003S 200 rxns 105 In den Shop wechseln Produktinformationen
M3003L 500 rxns 237 € In den Shop wechseln Produktinformationen
M3003X 1.000 rxns 424 € In den Shop wechseln Produktinformationen
M3003E 2.500 rxns 948 € In den Shop wechseln Produktinformationen
Luna® Universal Probe qPCR Master Mix M3004S 200 rxns 88 € In den Shop wechseln Produktinformationen
M3004L 500 rxns 198 € In den Shop wechseln Produktinformationen
M3004X 1.000 rxns 364 € In den Shop wechseln Produktinformationen
M3004E 2.500 rxns 812 € In den Shop wechseln Produktinformationen
Luna® Universal One-Step RT-qPCR Kit E3005S 200 rxns 218 € In den Shop wechseln Produktinformationen
E3005L 500 rxns 498 € In den Shop wechseln Produktinformationen
E3005X 1.000 rxns 869 € In den Shop wechseln Produktinformationen
E3005E 2.500 rxns 1.924 € In den Shop wechseln Produktinformationen
Luna® Universal Probe One-Step RT-qPCR Kit E3006S 200 rxns 198 € In den Shop wechseln Produktinformationen
E3006L 500 rxns 448 € In den Shop wechseln Produktinformationen
E3006X 1.000 rxns 788 € In den Shop wechseln Produktinformationen
E3006E 2.500 rxns 1.738 € In den Shop wechseln Produktinformationen
LunaScript RT SuperMix Kit E3010S 25 rxns 125 € In den Shop wechseln Produktinformationen
E3010L 100 rxns 420 € In den Shop wechseln Produktinformationen

Affordable Performance cDNA Synthesis:
NEBs ProtoScript II Reverse Transcriptase

ProtoScript II Reverse Transcriptase is a recombinant M-MuLV reverse transcriptase with reduced RNase H activity and increased thermostability. It can be used to synthesize first strand cDNA at higher temperatures than the wild-type M-MuLV. The enzyme is active up to 48°C, providing higher specificity, higher yield of cDNA and more full-length cDNA product, up to 12 kb in length.

Further product information

Jurkat total RNA (1 μg) was used in a 20 μl first strand cDNA synthesis …read more

PRODUCT Prod. # SIZE PRICE
ProtoScript® II First Strand cDNA Synthesis Kit E6560S 30 reactions 162 € In den Shop wechseln Produktinformationen
E6560L 150 reactions 648 € In den Shop wechseln Produktinformationen
ProtoScript® II Reverse Transcriptase M0368S 4.000 units 80 € In den Shop wechseln Produktinformationen
M0368L 10.000 units 160 € In den Shop wechseln Produktinformationen
M0368X 40.000 units 574 € In den Shop wechseln Produktinformationen

Get more of what you need, with the highest library yields:
NEBNext Ultra II RNA for Illumina

Do you need increased sensitivity and specificity from your RNA-seq experiments? Do you have ever-decreasing amounts of input RNA? To address these challenges, our next generation of RNA library prep kits have been reformulated at each step, resulting in several fold higher yields of high quality libraries, enabling use of lower input amounts and fewer PCR cycles.

Further product information
UltraII_Dir_RNA_yield

Poly(A)-containing mRNA was isolated from 10 ng, 100 ng and 1 µg of Universal Human Reference RNA …read more

PRODUCT Prod. # SIZE PRICE
NEBNext® Ultra™ II Directional RNA Library Prep Kit Illumina E7760S 24 rxns 1.028 € In den Shop wechseln Produktinformationen
E7760L 96 rxns 3.495 € In den Shop wechseln Produktinformationen
NEBNext® Ultra™ II Directional RNA Library Prep with Beads E7765S 24 rxns 1.140 € In den Shop wechseln Produktinformationen
E7765L 96 rxns 3.880 € In den Shop wechseln Produktinformationen
NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® E7770S 24 rxns 980 € In den Shop wechseln Produktinformationen
E7770L 96 rxns 3.325 € In den Shop wechseln Produktinformationen
NEBNext® Ultra™ II RNA Library Prep with Purification Beads E7775S 24 rxns 1.080 € In den Shop wechseln Produktinformationen
E7775L 96 rxns 3.685 € In den Shop wechseln Produktinformationen

How low can you go?
NEBNext Single Cell/Low Input RNA Library Prep

This unique workflow meets the demand for a highly sensitive, yet robust method that consistently generates high-quality, full-length transcript sequencing data from a single cell or ultra-low input RNA.
Optimized cDNA synthesis and amplification steps incorporate template switching, as well as utilize a unique protocol and suite of reagents. Even low-abundance transcripts are represented in the high yields of cDNA obtained. Subsequent library construction incorporates the Ultra™ II FS enzymatic DNA fragmentation/end repair/dA-tailing mix in a simple and efficient workflow.

Further product information
NEBNext Single Cell Low Input RNA yields

Generate higher library yields with the NEBNext Single Cell/Low Input RNA Library Prep Kit …read more

PRODUCT Prod. # SIZE PRICE
NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina E6420S 24 rxns 1.195 € In den Shop wechseln Produktinformationen
E6420L 96 rxns 3.965 €
NEBNext Single Cell/ Low Input cDNA Synthesis & Amplification Module E6421S 24 rxns 650 € In den Shop wechseln Produktinformationen
E6421L 96 rxns 2.210 €
NEBNext Multiplex Oligos for Illumina
(Index Primers Set 1, 2, 3, 4)
E7335S/L, E7500S/L, E7710S/L, E7730S/L 24 / 96 rxns 106 € / 384 € In den Shop wechseln
NEBNext Multiplex Oligos for Illumina (96 Index Primers) E6609S 96 rxns 680 € In den Shop wechseln Produktinformationen
E6609L 384 rxns 2.720 €
NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1, 2) E7600S, E7780S 96 rxns 461 € In den Shop wechseln Produktinformationen
NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) E6440S/L 96 / 384 rxns 535 € / 1.925 € In den Shop wechseln Produktinformationen

More Information on RNA modification and RNA modifying enzymes