EMBL Course – Enzymatic Methyl-seq: Bisulfite-Free Methylation Analysis

We are happy to announce the wet-lab course “Enzymatic Methyl-seq: Bisulfite-Free Methylation Analysis”.

The course will be held from 2 – 5 December 2019

at the EMBL in Heidelberg, Germany, as a part of the EMBL Corporate Partnership Program.

Application has been closed by September 27, 2019. More information on the course can be found on the EMBL event page.

Course Overview

The aim of this course is to prepare and analyse “bisulfite-free” methylome libraries for Illumina sequencing. We will use a novel enzymatic approach to detect 5-methyl cytosines (5mC’s), that results in longer reads and higher coverage of genomic features at lower minimum coverage thresholds when compared to Illumina bisulfite libraries.

Audience

This course is directed towards wet-lab biologists, scientists and technicians who want to apply the bisulfite-free method for detecting 5mC’s to their samples. Participants will learn the best practice of library preparation and data analysis. Knowledge of next generation sequencing technology is desirable but not essential.

Modules/Resources

Enzymatic detection of 5mC’s
NGS
Introduction to methylome data analysis

Learning Outcomes

Preparation of methylome libraries using an enzymatic approach. Introduction to data analysis of these libraries.