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NEW: LUNA SARS-CoV-2 RT-qPCR Multiplex Assay Kit
The Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit allows for the real-time detection of SARS-CoV-2 nucleic acid* by RT-PCR. The kit contains primers and probes specific for two regions of the virus N gene. The probes are labeled with two different fluorophores (N1: HEX; N2: FAM) to enable simultaneous detection of both signals. Multiplex-detection enables high throughput workflows.
- Reduced background amplification from gDNA using a modified RNase P internal control reverse primer
- LUNA WarmStart technology allows room temperature setup
- Reduced risk of carryover contamination with thermolabile UDG and dUTP in the master mix
- Supports sample pooling with minimal loss in sensitivity
* Research Use Only
Using the Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit, up to 94 different samples can be assessed in a single 96-well plate. Anticipated results for each sample type are shown (in each fluorophore channel).
SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit
LAMP (Loop-mediated isothermal amplification) is an attractive alternative to laborious PCR. Our scientist have developed a LAMP-assay with a colorimetric detection which is more reliable, faster and more sensitive than ever before. So direct SARS-CoV-2 detection in only about 30 min becomes a reality*.
- rapid, isothermal assay for reliable detection of Sars-CoV-2 in 30 min*
- Visual cue to check directly for successful amplification: as color changes from pink to yellow
- Excellent specificity and sensitivity due to optimized LAMP primers for the N- and the E-region of the SARS-CoV-2 genome
- Convenient reaction set-up at room temperature using WarmStart technology
- Efficient protection from carryover contamination through the use uf UDG/dUTP
- Kit incl. primer sets and positive control
* Research Use Only
Tip: To increase thoughput of colorimetric LAMP assays, one can also use absorbance plate readers. As an example, please find here an exciting, corresponding Application Note by BMG Labtech:
NEBNext ARTIC SARS-CoV-2 Library Prep Kits
Developed in collaboration with the ARTIC network, NEBNext ARTIC kits use a multiplexed amplicon-based whole-viral genome sequencing approach for sequencing SARS-CoV-2 using Oxford Nanopore Technologies® or Illumina® Sequencing.
- Improved uniformity of SARS-CoV-2 genome coverage depth
- Streamlined, high-efficiency protocols
- Effective with a wide range of viral genome inputs (10-10,000 copies)
- Available for Illumina and Oxford Nanopore Technologies sequencing platforms
- Single RT conditions for all input amounts
- No requirement for amplicon normalization prior to library preparation (Illumina-compatible kits)
- Optional use control human primers provided
- Includes NEBNext Sample Purification Beads (SPRIselect®)
- Library adaptors and primers available separately
* Research Use Only
NEB reagents have been cited in more than 1400 publications, pre-prints and EUA protocols since the beginning of the corona-virus pandemic. We provide you with the necessary reliability and accuracy not only by delivering our products on-time but also by offering excellent technical support.
Thus, choose NEB products for:
Selected current publications and pre-prints on Covid-19 research with NEB products:
Local emergence and decline of a SARS-CoV-2 variant with mutations L452R and N501Y in the spike protein
J-P. Mallm et al., MedRxiv 2021, April 29
Researchers from Heidelberg around Prof. Kräusslich and Prof. Rippe describe the occurrence of different mutations in the surface protein of SARS-CoV-2 in the Rhine-Neckar region in Baden-Wuerttemberg, Germany. For this purpose, the researchers use the NEBNext ARTIC SARS-CoV-2 FS Library Prep Kit (Illumina) (#E7658). They found distinct mutations in 166 positive samples that can be clustered into 8 groups. Of these, a novel A.27.RN mutation initially appeared dominant, but was then apparently repressed by B.1.1.7. This study demonstrates the need for continuous and comprehensive virus monitoring at the population level.
Rapid point-of-care detection of SARS-CoV-2 using reverse transcription loop-mediated isothermal amplification (RT-LAMP)
Lena Mautner et al., Virol J 17, 160 (2020)
Lena Mautner et al. report the development of a loop-mediated isothermal amplification (LAMP) based method to detect SARS-CoV-2 genes ORF8 and N directly from pharyngeal swab samples. The established reverse transcription LAMP (RT-LAMP using NEB’s #E1700 Kit) assay detects SARS-CoV-2 directly from pharyngeal swab samples without previous time-consuming and laborious RNA extraction. The assay is ideally suited for POCT at e.g. railway stations, airports or hospitals. Given the current pandemic situation, rapid, cost efficient and onsite methods like the here presented RT-LAMP assay are urgently needed to contain the viral spread.
Enhancing colorimetric loop-mediated isothermal amplification speed and sensitivity with guanidine chloride
Yinhua Zhang, et al. (New England Biolabs), Biotechniques 2020 Jul 8.
Learn how NEB optimized Colorimetric Covid-19 LAMP Assay using new Primersets and Guanidine Hydrochlorid. This Paper is the basis for the new SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit (#E2019)
A community-deployable SARS-CoV-2 screening test using raw saliva with 45 minutes sample-to-results turnaround
Meyerson, N. et. al., MedRxiv 2020 Jul 17.
Meyerson et. al from the university of colorado provide a comprehensive protocol (from saliva sample to data analysis) to perform reliable, rapid SARS-CoV-2 testing directly from saliva samples in as little as 45 min using NEBs colorimetric LAMP mastermix
Screening for SARS-CoV-2 infections with colorimetric RT-LAMP and LAMP sequencing
Dao Thi et al., Science Translational Medicine 12 Aug 2020, Vol. 12, Issue 556
Universtity Hospital and DKFZ in Heidelberg establish Colorimetric LAMP for Covid-19 surveillance testing. Sensitivity of LAMP on extracted RNA and unpurified swab samples are compared.
Scalable, rapid and highly sensitive isothermal detection of SARS-CoV-2 for laboratory and home testing
Kellner et al., BioRxiv 2020 Jun 23.
Scientists at the IMP Vienna establish a sensitive and robust colorimetric LAMP assay with NEB Bst Polymerases, reverse Transcriptase RTx and UDG. The combination is now available as convenient mastermix format (M1804). Optimizations include: multiplex primersets, a quick extraction/enrichment protocol with carboxy-beads (Bead-LAMP) and the alternative dye hydroxy-naphtol-blue HNB for colorimetric detection.
LAMP-Seq: Population-Scale COVID-19 Diagnostics Using Combinatorial Barcoding
Jonathan Schmid-Burgk et al., BioRxiv 2020 Jun 08.
The University Hospital in Bonn develops a promising approach for population-wide testing. Automated RT-LAMP with NEB’s Mastermix includes sample-specific barcodes, reactions are massively pooled and amplified barcodes (i.e. positive samples) are identified by next generation sequencing.
Rapid SARS-CoV-2 testing in primary material based on a novel multiplex LAMP assay
Bernhard Schermer et al., MedRxiv 2020 Jun 22.
The group at the University Hospital Cologne published a LAMP assay for Covid-19 detection, optimized by multiplex primer sets for detection and compares LAMP with Cas-Nuclease-based SHERLOCK Assay. The more sensitive LAMP assay is then discussed as prime-option for the development of easy and cheap POC test kits.
Multiplexed RT-qPCR to screen for SARS-COV-2 B.1.1.7, B.1.351, and P.1 variants of concern V.3
Chantal Vogels et.al., Department of Epidemiology of Microbial Diseases, Yale School of Public Health, Protocols.io; Feb. 9th, 2021
COVID-19 RT-qPCR Testing Pipeline – complete protocol
Heinen, R. et. al., Vienna COVID-19 Detection Initiative (VCDI).
In the course of the Vienna COVID-19 initiative Robert Heinen, Petra Pjevac, Nikolaus Beer, Harald Scheuch and Johannes Zuber have established a comprehensive protocol from sample collection and preparation (including saliva and gargle samples) to qPCR detection. In this protocol the NEB Luna Universal Probe One-Step RT-qPCR Kit (E3007) is recommended as it “(…) is attractively priced and performs among the most sensitive One-step kits we tested.”
SalivaDirect: Simple and sensitive molecular diagnostic test for SARS-CoV-2 surveillance
Vogels, C. et. al., MedRxiv 2020 Aug 08.
At Yale university, researchers have developed a FDA-EUA-certified protocol for dualplex RT-qPCR directly from saliva samples without RNA extractions. In this protocol all necessary components from various manufacturers are described in detail, including comprehensive working instructions. In addition to proteinase K which is used for sample preparation, also NEBs Luna One-Step Probe RT-qPCR Kit (#E3006) is utilized for detection.
A direct RT-qPCR approach to test large numbers of individuals for SARS-CoV-2
Maricic et al., MedRxiv 2020 Jun 26.
Authors from the MPI for evolutionary Anthropology in Leipzig present a cheap and fast approach to detect SARS-CoV-2 in single or pooled gargle lavages (‘mouthwashes’), using NEBs Luna Universal RT-qPCR Mastermix. Direct RT-qPCR of pooled mouthwashes is discussed as a scalable option for surveillance testing.
High-throughput qPCR and RT-qPCR Workflows Enabled by Beckman Coulter Echo Acoustic Liquid Handling and NEB Luna Reagentss
Gray et. al., New England Biolabs Inc.
In this technical note, our colleagues explain how you can easily and reliably automate NEBs Luna RT-qPCR reagents to increase your throughput (384-well scale).
Optimized qRT-PCR approach for the detection of intra- and extra-cellular SARS-CoV-2 RNAs
Toptan, T. et. al., BioRxiv, 2020 April 25.
Scientist from the university hospital Frankfurt and GenXPro established new targets for SARS-CoV-2 detection and compared different RT-qPCR kits. The SARS-CoV-2 M-gene is detected particularly sensitive in clinical samples using NEBs Luna Universal Probe OneStep RT-qPCR kit.