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Supporting COVID-19 Research: (Customer)Publications and Protocols

General information

New England Biolabs is working diligently to ensure we keep our employees and their families safe, while maintaining our business continuity in support of our customers.

We are following the guidelines of local and national health organizations in order to mitigate the spread of COVID-19.

Our manufacturing and distribution teams continue to be fully operational!
We are working closely with our suppliers and logistic partners to ensure uninterrupted access to our products. Our technical support and customer service is also there for you as usual.

If you have any questions or suggestions, please feel free to contact us at info.de@neb.com.

We are thinking of you during this challenging time and hope you, your families and colleagues are in good health.

Sincerely yours,
NEB team

NEW: SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit

The SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit utilizes isothermal amplification for use in the analysis of SARS-CoV-2, the novel coronavirus that causes COVID-19.

Advantages:

  • Colorimetric LAMP enables simple, visual detection (pink-to-yellow) of amplification of SARS-CoV-2 nucleic acid
  • Set up reactions quickly and easily, using a simple heat source and unique WarmStart technology
  • Reduce risk of carryover contamination, with UDG and dUTP included in the master mix
  • Assay targets N and E regions of the SARS-CoV-2 genome, for optimized sensitivity and specificity
  • Bring confidence to your results using the provided controls
Learn more!

* Research Use Only

Learn more about how NEB is supporting COVID-19 research!

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Selected current publications and pre-prints on Covid-19 research with NEB products:

Facilitating Detection of SARS-CoV-2 Directly from Patient Samples: Precursor Studies with RT-qPCR and Colorimetric RT-LAMP Reagents
Andrew N. Gray, Ph.D., et al. (New England Biolabs)

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Enhancing colorimetric loop-mediated isothermal amplification speed and sensitivity with guanidine chloride
Yinhua Zhang, et al. (New England Biolabs), Biotechniques 2020 Jul 8.

Learn how NEB optimized Colorimetric Covid-19 LAMP Assay using new Primersets and Guanidine Hydrochlorid. This Paper is the basis for the new SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit (#E2019)

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A direct RT-qPCR approach to test large numbers of individuals for SARS-CoV-2
Maricic et al., MedRxiv 2020 Jun 26.

Authors from the MPI for evolutionary Anthropology in Leipzig present a cheap and fast approach to detect SARS-CoV-2 in single or pooled gargle lavages (‘mouthwashes’), using NEBs Luna Universal RT-qPCR Mastermix. Direct RT-qPCR of pooled mouthwashes is discussed as a scalable option for surveillance testing.

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Scalable, rapid and highly sensitive isothermal detection of SARS-CoV-2 for laboratory and home testing
Kellner et al., BioRxiv 2020 Jun 23.

Scientists at the IMP Vienna establish a sensitive and robust colorimetric LAMP assay with NEB Bst Polymerases, reverse Transcriptase RTx and UDG. The combination is now available as convenient mastermix format (M1804). Optimizations include: multiplex primersets, a quick extraction/enrichment protocol with carboxy-beads (Bead-LAMP) and the alternative dye hydroxy-naphtol-blue HNB for colorimetric detection.

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Rapid SARS-CoV-2 testing in primary material based on a novel multiplex LAMP assay
Bernhard Schermer et al., MedRxiv 2020 Jun 22.

The group at the University Hospital Cologne published a LAMP assay for Covid-19 detection, optimized by multiplex primer sets for detection and compares LAMP with Cas-Nuclease-based SHERLOCK Assay. The more sensitive LAMP assay is then discussed as prime-option for the development of easy and cheap POC test kits.

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LAMP-Seq: Population-Scale COVID-19 Diagnostics Using Combinatorial Barcoding
Jonathan Schmid-Burgk et al., BioRxiv 2020 Jun 08.

The University Hospital in Bonn develops a promising approach for population-wide testing. Automated RT-LAMP with NEB’s Mastermix includes sample-specific barcodes, reactions are massively pooled and amplified barcodes (i.e. positive samples) are identified by next generation sequencing.

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Screening for SARS-CoV-2 infections with colorimetric RT-LAMP and LAMP sequencing
Dao Thi et al., Science Translational Medicine  12 Aug 2020, Vol. 12, Issue 556

Universtity Hospital and DKFZ in Heidelberg establish Colorimetric LAMP for Covid-19 surveillance testing. Sensitivity of LAMP on extracted RNA and unpurified swab samples are compared.

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