NEBNext Direct Cancer HotSpot Panel

NEBNext Direct Target Enrichment

NEBNext Direct employs a unique technology that enables highly specific target enrichment of genomic regions of interest. The result of a partnership between New England Biolabs and Directed Genomics™, this innovative approach to target enrichment balances the speed and precision of multiplexed PCR-based approaches with the content scalability typical of hybridization-based methods. This flexibility allows a single workflow for assays ranging from single gene tests to comprehensive panels including several hundred genes. Regardless of sample type or assay content, NEBNext Direct allows you to enrich your targets with precision.

Workflow

NEBNext Direct Workflow

What people are saying

„Using the NEBNext Direct kit, we were able to detect all known single nucleotide variants and indels in DNA extracted from fresh frozen or FFPE tissue derived from glioma biopsies. We could also clearly see amplification in genes like EGFR or PDGFR. The workflow is really easy and fast and can be rapidly implemented in a lab.“

Yannick Marie, Sequencing Core Facility Manager, Brain and Spine Institute (ICM)

„The kit and its technology are easy to use and easy to automate, allowing us to get up and running quickly. The protocol itself is fast and efficient to obtain deep coverage of targets, giving homogeneous results for FFPE and frozen tumors, therefore opening doors for customized panels.“

Francis Rousseau, Ph.D., Director of Genomics for IntegraGen SA

Videos

NEBNext Direct Workflow

View our tutorial for help for visualizing NEBNext Direct's unique and enabling workflow.

Protocol for Target Enrichment with NEBNext Direct

Part 1 of 3
Introduction Through Bead Preparation

This series of videos walks you through enrichment of genomic DNA for library preparation using the NEBNext Direct Cancer HotSpot Panel for Illumina Sequencing.

Protocol for Target Enrichment with NEBNext Direct

Part 2 of 3
Bead Binding Through Adaptor Ligation
This series of videos walks you through enrichment of genomic DNA for library preparation using the NEBNext Direct Cancer HotSpot Panel for Illumina Sequencing

Protocol for Target Enrichment with NEBNext Direct

Part 3 of 3
Adaptor Cleaving Through Purification of Fragments
This series of videos walks you through enrichment of genomic DNA for library preparation using the NEBNext Direct Cancer HotSpot Panel for Illumina Sequencing.
NEB TV Folge 11

NEB TV – Episode 11

Precision medicine requires precision enrichment. Learn more about target enrichment and its role in clinical applications.

NEBNext Direct Target Enrichment

Learn about the innovative NEBNext Direct technology for target enrichment for next generation sequencing.

Read also the FEATURE ARTICLE on:
“Overcoming the challenges of applying target enrichment for translational research”
by Andrew Barry, MS, Product Expert at NEB Inc.

DOWNLOAD PDF (1.7MB)

Available products:

NEBNext Direct® Custom Ready Panels

NEBNext Direct Custom Ready Panels allow rapid customization of targeted gene panels for Illumina sequencing. Select from a list of genes for which baits have been carefully designed and optimized to give complete coverage of the full coding regions. High quality panels can be designed by you and rapidly delivered, from any combination of genes. NEBNext Direct Custom Ready Panels provide the content you want with the performance you need.

More information
Advantages:
  • Choose from a single gene to hundreds of genes
  • Experience unmatched specificity and coverage uniformity
  • Eliminate synthesis and optimization steps for faster turnaround
  • Improve sensitivity with our Unique Molecule Index (UMI)
  • Generate results in one day with our automation-friendly workflow

NEBNext Direct® Cancer HotSpot Panel for Target Enrichment

The NEBNext Direct Cancer HotSpot Panel contains baits that capture both strands of DNA across 190 common cancer targets from 50 genes, encompassing approximately 40 kb of sequence and including over 18,000 COSMIC features (Table 1). The panel is designed to generate targets of roughly 150 bp, compatible with PE75 Illumina sequencing.

Advantages:
  • Generate a higher percentage of your sequencing reads aligning to your targets
  • Eliminate the need to over-sequence, reducing cost per sample
  • Obtain uniform sequencing of ALL targets, regardless of GC content
  • Save time with a streamlined workflow that couples enrichment and library preparation
  • Generate high quality libraries with limited input amounts and degraded DNA samples, including FFPE and ctDNA
  • Distinguish molecular duplicates, reducing false positive variants and improving sensitivity
  • On-bead sample preparation is ideal for automated workflows

NEBNext Direct® BRCA1/BRCA2 Panel

The NEBNext Direct BRCA1/BRCA2 Panel enriches the complete exon content of BRCA1 and BRCA2 genes for next-generation se­quencing analysis. NEBNext Direct employs a unique hybridization-based enrichment workflow that hybridizes baits directly to genomic DNA, without the need for upfront library preparation. The BRCA1/BRCA2 panel demonstrates extremely high specificity and unmatched coverage uniformity across a wide range of DNA inputs, allowing highly sensitive calling of germline and somatic variants while maximizing sequencer efficiency.

The NEBNext Direct BRCA1/BRCA2 Panel delivers highly efficient enrichment of BRCA1 and BRCA2 coding regions with a high percentage of reads mapping to targets

Advantages:
  • Generate full (100%) coverage of all protein coding regions in BRCA1 and BRCA2 genes
  • Obtain highly uniform sequencing across exon targets- 100% of basepairs in panel have coverage greater than 20% of the mean target coverage.
  • Maximize efficiency with a 1-day workflow that combines highly specific enrichment with library preparation
  • Produce high depths of target coverage across a wide range of DNA input amounts for germline and somatic variant calling.

Product table

PRODUCT Prod. # SIZE PRICE
NEW: NEBNext Direct® Custom Ready Panels E6631S 8 rxns on request; dependent on panel content Produktinformationen
E6631L 24 rxns
E6631X 96 rxns
NEBNext Direct® Cancer HotSpot Panel E7000S 8 rxns 1.450 € In den Shop wechseln Produktinformationen
E7000L 24 rxns 4.200 € In den Shop wechseln Produktinformationen
E7000X 96 rxns 11.180 € In den Shop wechseln Produktinformationen
NEBNext Direct® BRCA1/BRCA2 Panel E6627S 8 rxns 660 € In den Shop wechseln Produktinformationen
E6627L 24 rxns 1.900 € In den Shop wechseln Produktinformationen
E6627X 96 rxns 6.950 € In den Shop wechseln Produktinformationen

As of: 03.01.2019

NEBNext products are available at a permanent low price that can not be further discounted.

You have further questions and/or would like a sample? Please contact our Application Specialist:

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Learn more about NEBNext Direct and this innovative
approach to target enrichment for next-generation sequencing.

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Further information can be found in our Technical Resources section or at neb.com. Information on trademarks can be found here.