NEW: NEBNext Direct Custom Ready Panels

Employing the unique NEBNext Direct hybridization-based enrichment method, NEBNext Direct Custom Ready Panels allow rapid customization of targeted gene panels for Illumina sequencing. Select from a list of genes for which baits have been carefully designed and optimized to give complete coverage of the full coding regions. High quality panels can be designed by you and rapidly delivered, from any combination of genes. NEBNext Direct Custom Ready Panels provide the content you want with the performance you need.

Click here for more information on the NEBNext Direct Target Enrichment technology

Advantages:
  • Choose from a single gene to hundreds of genes
  • Experience unmatched specificity and coverage uniformity
  • Eliminate synthesis and optimization steps for faster turnaround
  • Improve sensitivity with our Unique Molecule Index (UMI)
  • Generate results in one day with our automation-friendly workflow
NEB Next Direct Target Enrichment Performance 1

NEBNext Direct Custom Ready Panels demonstrate optimum performance across a wide range of panel sizes

Key target enrichment metrics demonstrate consistent performance across a range of panel sizes. 100 ng of DNA was tested against panels of 1, 10, 25, 50 and 100 genes, and sequenced using Illumina paired-end 150 bp sequencing. Larger panels included all genes present in smaller panels.

NEB Next Direct Target Enrichment Performance 2

Sensitivity in detection of variants across panel size and DNA input amount

24 HapMap samples were blended to create a range of variant allele frequencies (VAF) down to 2%. 25, 50, 100, 200 and 500 ng of this blended DNA was enriched using NEBNext Direct Custom Ready Panels of 1, 10, 25, 50, and 100 genes. Larger panels were inclusive of the genes in smaller panels. Resulting libraries were sequenced using 2 x 150 bp Illumina sequencing and variants were called using Mutect and Vardict variant calling algorithms.

NEB Next Direct Target Enrichment Performance 3

NEBNext Direct Custom Ready Panels demonstrate retention of target behavior across panel sizes

IGV image of coverage profile for 4 BRAF exons included in panels of 1, 10, 25, 50 and 100 genes, demonstrate consistent target behavior with the addition of gene targets. 100 ng of DNA was used as input for NEBNext enrichment using the 5 panels, including the BRAF gene. Libraries were sequenced on an Illumina 2 x 150 basepair sequencing.

To the Panel Configurator

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Product table

PRODUCT Prod. # SIZE PRICE
NEU: NEBNext Direct® Custom Ready Panels E6631S 8 rxns auf Anfrage; abhängig von Panelinhalt Produktinformationen
E6631L 24 rxns
E6631X 96 rxns

As of: 01.01.2021

NEBNext products are available at a permanent low price that can not be further discounted.

Further information can be found in our Technical Resources section or at neb.com. Information on trademarks can be found here.