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NEBNext Ultra II DNA Kit and Modules

The new benchmark in NGS Library Prep for Illumina Platforms.

Get even more from less with NEBNext Ultra II!

Are you challenged with trying to obtain higher library yields using ever-decreasing input amounts? Each component in the NEBNext Ultra II DNA Library Prep Kit from NEB® has been reformulated, resulting in a several-fold increase in library yield with as little as 500 picograms of input DNA. These advances deliver unprecedented performance, while enabling lower inputs and fewer PCR cycles.

Find all technical information on NEBNext Ultra II Kits here:

Technical Details
Advantages:
  • Get more of what you need, with the highest library yields
  • Use to generate high quality libraries even when you have only limited amounts of DNA, with inputs as low as 500 pg
  • Prepare libraries from ALL of your samples, including GC-rich targets and FFPE DNA samples
  • Improve yields for target enrichment applications
  • Save time with streamlined workflows, reduced hands-on time, and automation compatibility, and enjoy the flexibility of kit or module format products

NEBNext Ultra II produces the highest yield libraries from a broad range of input amounts.

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NEBNext Ultra II DNA Library Prep Protocol

This video walks you through DNA Library Preparation using the NEBNext Ultra II DNA Library Prep Kit. The video also includes tips for optimization as well as safe stopping points.

12 Quick Tips for NGS Library Preparation

Using beads to clean up your DNA prior to NGS library prep can be quick and easy, if you follow these few, simple tips.

More Video Tutorials on NEB’s YouTube Channel.

See what others are saying about NEBNext Ultra II:

„The possibility to use the new NEBNext Ultra II DNA kit has provided us with a critical opportunity to process challenging low-input genomic samples. These samples otherwise didn't yield libraries of adequate complexity, required for exploring their genomes comprehensively. Using the NEBNext Ultra II DNA Kit has alleviated this problem and enabled us to deliver high quality, high content sequencing data that are relevant for our users.“

Dr. Vladimir Benes, Head of GeneCore, EMBL Heidelberg

„The protocol is very easy to follow and is a step up from the standard NEB Ultra Kit. Using as little as 500 pg of cell-free DNA input, we were able to generate libraries that were successfully used for subsequent exome capture.“

Dr. Christopher Smith, Post-Doctoral Research Associate at the Cancer Research UK - Cambridge Institute (CRUK-CI)

Brochure:

NEBNext for Illumina – NGS Sample Preparation

Download

Technical Note:

“Improved library preparation with the
NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®

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*Samples are limited to 1 piece per working group and as long as stock lasts.

Product table

PRODUCT Prod. # SIZE PRICE
NEBNext® Ultra II DNA Library Prep Kit for Illumina® E7645S 24 rxns 535 € In den Shop wechseln Produktinformationen
E7645L 96 rxns 2.045 € In den Shop wechseln Produktinformationen
NEBNext® Ultra™ II DNA Library Kit with Purification Beads E7103S 24 rxns 615 € In den Shop wechseln Produktinformationen
E7103L 96 rxns 2.352 € In den Shop wechseln Produktinformationen
NEBNext® Ultra II End Repair/dA-Tailing Module E7546S 24 rxns 262 € In den Shop wechseln Produktinformationen
E7546L 96 rxns 835 € In den Shop wechseln Produktinformationen
NEBNext® Ultra II Ligation Module E7595S 24 rxns 395 € In den Shop wechseln Produktinformationen
E7595L 96 rxns 1.270 € In den Shop wechseln Produktinformationen
NEBNext® Ultra II Q5® Master Mix M0544S 50 rxns (50 µl/vol) 99 € In den Shop wechseln Produktinformationen
M0544L 250 rxns (50 µl/vol) 395 € In den Shop wechseln Produktinformationen

As of: 03.01.2019

NEBNext products are available at a permanent low price that can not be further discounted.

Further information can be found in our Technical Resources section or at neb.com. Information on trademarks can be found here.