NEBNext Ultra II RNA Library Prep Kit and Modules

Now available: NEBNext® Ultra™ II RNA Library Prep

Do you need increased sensitivity and specificity from your RNA-seq experiments? Do you have ever-decreasing amounts of input RNA? To address these challenges, our next generation of RNA library prep kits have been reformulated at each step, resulting in several fold higher yields of high quality libraries, enabling use of lower input amounts and fewer PCR cycles.

Our new NEBNext Ultra II RNA kits have streamlined, automatable workflows and are available for directional (strand-specific, using the “dUTP method”) and non-directional library prep, with the option of SPRIselect® beads for size-selection and clean-up steps.

Test the unequaled performance with your own material and experience the advantages of NEBNext Ultra II!

Why consider the NEBNext Ultra II Directional RNA Library Prep Kit and Ultra II RNA Library Prep Kit for Illumina?

  • Get more of what you need, with the highest library yields
  • Generate high quality libraries even when you have only limited amounts of input RNA:
    • 10 ng – 1 µg Total RNA (polyA mRNA workflow)
    • 5 ng – 1 µg Total RNA (rRNA depletion workflow)
  • Minimize bias, with fewer PCR cycles required
  • Maximize the flexibility to order reagents for your specific workflow needs
    • Directional (strand-specific, using the “dUTP method”) and non-directional workflow options
    • rRNA Depletion and poly(A) mRNA Isolation reagents
    • Adaptors and primers for multiplexing (12- and 96-index) formats
  • Enjoy the reliability of the gold standard SPRIselect size selection and clean-up beads, supplied in just the amounts you need
  • Optimize your time with streamlined workflows, reduced hands-on time, and automation compatibility
  • Rely on robust performance, even with low quality RNA
UltraII_Dir_RNA_yield

Poly(A)-containing mRNA was isolated from 10 ng, 100 ng and 1 µg of Universal Human Reference RNA (Agilent #740000) and libraries were made using the NEBNext Ultra II Directional RNA kit, Kapa Stranded mRNA-Seq kit, Kapa mRNA HyperPrep kit and Illumina TruSeq Stranded mRNA Kit. The input RNA amount and number of PCR cycles are indicated. Library yields from an average of three replicates are shown. Error bars indicate standard deviation. Library yields were assessed using the Agilent® Bioanalyzer®.

NEBNext Ultra II Products are easily automatable and have been implemented on diverse platforms.

Product table

PRODUCT Prod. # SIZE PRICE
NEBNext® Ultra™ II Directional RNA Library Prep Kit Illumina E7760S 24 rxns 1.028 € In den Shop wechseln Produktinformationen
E7760L 96 rxns 3.495 € In den Shop wechseln Produktinformationen
NEBNext® Ultra™ II Directional RNA Library Prep with Beads E7765S 24 rxns 1.140 € In den Shop wechseln Produktinformationen
E7765L 96 rxns 3.880 € In den Shop wechseln Produktinformationen
NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® E7770S 24 rxns 980 € In den Shop wechseln Produktinformationen
E7770L 96 rxns 3.325 € In den Shop wechseln Produktinformationen
NEBNext® Ultra™ II RNA Library Prep with Purification Beads E7775S 24 rxns 1.080 € In den Shop wechseln Produktinformationen
E7775L 96 rxns 3.685 € In den Shop wechseln Produktinformationen

As of: 03.01.2019

NEBNext products are available at a permanent low price that can not be further discounted.

You would like to test the NEBNext Ultra II RNA Kit? Please contact us for a sample*:

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Testmusteranfrage

(Pflichtfelder sind mit einem * gekennzeichnet)

An welchem Testmuster sind Sie interessiert? *
NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® #E7760NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® #E7770

Die folgenden Reagenzien sind nicht in den Ultra II RNA Kits enthalten.
Fordern Sie die folgenden Reagenzien zusätzlich mit dem Kit an: *
NEBNext rRNA Depletion Kit #E6310NEBNext poly(A) mRNA Isolation Module #E7490NEBNext Multiplex Oligos #E7335

Welche Sequenzierplattform(en) benutzen Sie? *

IlluminaIon TorrentOxford Nanopore

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Klonierung & DNA-ModifikationNext-Gen-SequencingProteinexpression, Aufreinigung & ToolsSynthetische BiologieMicrobiomeZellanalyseDNA Amplifikation & PCRRNA AnalyseEpigenetikMutageneseGlykobiologieGenome Editing

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* NEBNext Testmuster sind ausschliesslich für qualifizierte Kunden verfügbar und nur solange der Vorrat reicht.
Wir kontaktieren Sie zur weiteren Vorgehensweise.

Ihre vollständigen Informationen zum Datenschutz, entsprechend den Anforderungen der DSGVO,
finden Sie unter www.neb-online.de/datenschutz

* NEBNext Samples are only available for qualified customers and as long as stocks last. We will get in contact with you for further details.

This content is covered by patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please email us at gbd@neb.com. The use of these products may require you to obtain additional third party intellectual property rights for certain applications.

KAPA® is a registered trademark of Kapa Biosystems
ILLUMINA® and TRUSEQ® are registered trademarks of Illumina, Inc.
AGILENT® and BIOANALYZER® are registered trademarks of Agilent Technologies
SPRISELECT® is a registered trademark of Beckman Coulter, Inc.

Further information can be found in our Technical Resources section or at neb.com. Information on trademarks can be found here.