{"id":10346,"date":"2016-10-13T08:14:51","date_gmt":"2016-10-13T06:14:51","guid":{"rendered":"http:\/\/www.neb-online.de\/en\/?page_id=10346"},"modified":"2023-12-19T15:45:57","modified_gmt":"2023-12-19T14:45:57","slug":"isothermal-amplification-polymerases","status":"publish","type":"page","link":"https:\/\/www.neb-online.de\/en\/pcr-and-dna-amplification\/isothermal-amplification-polymerases\/","title":{"rendered":"Isothermal Amplification \u2013 Polymerases"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column][vc_basic_grid post_type=&#8221;post&#8221; max_items=&#8221;10&#8243; element_width=&#8221;12&#8243; gap=&#8221;15&#8243; item=&#8221;4123&#8243; taxonomies=&#8221;70&#8243; grid_id=&#8221;vc_gid:1702997108643-121d7718-780c-9&#8243;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;2\/3&#8243;][vc_column_text]<\/p>\n<h2>Bst 2.0 DNA Polymerase<\/h2>\n<p><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=M0537\" target=\"_blank\" rel=\"noopener noreferrer\"><em>Bst\u00a0<\/em>2.0 DNA Polymerase<\/a> is an\u00a0<em>in silico<\/em>\u00a0designed homologue of\u00a0<em>Bacillus stearothermophilus<\/em>\u00a0DNA Polymerase I, Large Fragment (<a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=M0275\" target=\"_blank\" rel=\"noopener noreferrer\"><em>Bst<\/em>\u00a0DNA Polymerase, Large Fragment<\/a>).\u00a0<em>Bst\u00a0<\/em>2.0 DNA Polymerase contains 5\u00b4\u21923\u00b4 DNA polymerase activity and strong strand displacement activity but lacks 5\u00b4\u21923\u00b4 exonuclease activity.\u00a0<em>Bst\u00a0<\/em>2.0 DNA Polymerase displays improved amplification speed, yield, salt tolerance and thermostability compared to wild-type\u00a0<em>Bst<\/em>\u00a0DNA Polymerase, Large Fragment.<\/p>\n<h3>Bst 2.0 WarmStart\u2122 DNA Polymerase<\/h3>\n<p><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=M0538\" target=\"_blank\" rel=\"noopener noreferrer\"><em>Bst\u00a0<\/em>2.0 WarmStart DNA Polymerase<\/a> is an\u00a0<em>in silico<\/em>\u00a0designed homologue of\u00a0<em>Bacillus stearothermophilus\u00a0<\/em>DNA Polymerase I, Large Fragment (<em>Bst<\/em>\u00a0DNA Polymerase, Large Fragment) with a reversibly-bound aptamer, which inhibits polymerase activity at temperatures below 45\u00b0C. The aptamer rapidly releases the\u00a0<em>Bst<\/em>\u00a02.0 WarmStart DNA Polymerase above 45\u00b0C and therefore no special activation step is needed to activate the polymerase.[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/3&#8243;][vc_single_image image=&#8221;7159&#8243; img_size=&#8221;150&#215;189&#8243; alignment=&#8221;center&#8221;][vc_column_text]<\/p>\n<p style=\"text-align: center;\"><strong>\u201eImproved Reagents for<\/strong> <strong>Isothermal DNA Amplification\u201c<\/strong><\/p>\n<p style=\"text-align: center;\">Dr. Nathan Tanner, New England Biolabs Inc.<\/p>\n<p style=\"text-align: center;\"><b>Advantages:<\/b><\/p>\n<ul>\n<li style=\"text-align: center;\">&#8220;engineered for performance&#8221;<\/li>\n<li style=\"text-align: center;\">Faster<\/li>\n<li style=\"text-align: center;\">Increased thermostability<\/li>\n<li style=\"text-align: center;\">Improved salt tolerance<\/li>\n<li style=\"text-align: center;\">Higher yields<\/li>\n<\/ul>\n<p>[\/vc_column_text]<a class=\"btn btn-standard form-btn\" target=\"_blank\" href=\"\/wp-content\/uploads\/2015\/04\/NEB_isothermal_amp.pdf\" style=\"margin-left:auto;margin-right:auto;\">Download PDF<\/a>[\/vc_column][\/vc_row][vc_row][vc_column css=&#8221;.vc_custom_1448612431927{margin-top: 50px !important;}&#8221;][vc_separator color=&#8221;black&#8221;][vc_column_text]<\/p>\n<h2>New: <em>Bst<\/em> 3.0 DNA Polymerase \u2013 optimal for LAMP and RT-LAMP<\/h2>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row css=&#8221;.vc_custom_1448611320224{margin-right: 5px !important;}&#8221;][vc_column][vc_column_text]<em>Bst <\/em>3.0 DNA Polymerase is an <em>in silico<\/em> designed homologue of <em>Bacillus stearothermophilus<\/em> DNA Polymerase I, Large Fragment engineered for improved isothermal amplification performance and increased reverse transcription activity.<\/p>\n<p><em>Bst <\/em>3.0 DNA Polymerase contains 5\u00b4\u21923\u00b4 DNA polymerase activity with either DNA or RNA templates and strong strand displacement activity, but lacks 5\u00b4\u21923\u00b4 and 3\u00b4\u21925\u00b4 exonuclease activity.<\/p>\n<p><em>Bst <\/em>3.0 DNA Polymerase demonstrates robust performance even in high concentrations of amplification inhibitors and features significantly increased reverse transcriptase activity compared to <em>Bst <\/em>DNA Polymerase.<\/p>\n<p><strong>\u2013 in short: \u201eengineered for performance!\u201c<\/strong><\/p>\n<p><strong>The superior properties of <em>Bst<\/em> 3.0 DNA Polymerase make it a first choice enzyme for all of your (RT)-LAMP Experiments.<\/strong>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_single_image image=&#8221;6075&#8243; img_size=&#8221;full&#8221; alignment=&#8221;center&#8221; img_link_large=&#8221;yes&#8221;][vc_column_text]<strong>Fast, single-enzyme RT-LAMP can be performed using <em>Bst<\/em> 3.0 <\/strong> RT-LAMP was performed using indicated DNA polymerase and Jurkat total RNA and primers for two genes (ACTB, left; HMBS2, right). Fastest results were observed with a 2-enzyme system, Bst 2.0 and WarmStart RTx, but robust amplification was also observed using Bst 3.0 without additional RT. Bst LF, Bst 2.0 and competitor enzymes showed highly variable performance, with slow threshold times or reaction failure on one of the two targets.[\/vc_column_text][vc_separator color=&#8221;black&#8221;][\/vc_column][\/vc_row][vc_row css=&#8221;.vc_custom_1448620303836{margin-right: 5px !important;margin-left: 5px !important;padding-top: 20px !important;padding-bottom: 20px !important;background-color: #f4f1e7 !important;}&#8221;][vc_column][vc_row_inner][vc_column_inner width=&#8221;2\/3&#8243;][vc_column_text]<span style=\"font-size: 1.2em;\"><strong>Not sure which polymerase you need for<br \/>\nyour Isothermal Amplification?<\/strong><\/span>[\/vc_column_text][\/vc_column_inner][vc_column_inner width=&#8221;1\/3&#8243; css=&#8221;.vc_custom_1448620484630{padding-top: 25px !important;padding-right: 30px !important;}&#8221;]<a class=\"btn btn-standard form-btn\"  href=\"https:\/\/www.neb.com\/faqs\/2013\/10\/24\/which-dna-polymerase-is-best-for-my-isothermal-amplification-reaction\" style=\"margin-left:auto;margin-right:auto;\">Then click here<\/a>[\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=&#8221;black&#8221;][vc_row_inner][vc_column_inner width=&#8221;2\/3&#8243;][vc_column_text]<span style=\"font-size: 1.3em;\"><strong>We have an overview and background information on Isothermal Amplification in our current brochure.<\/strong><\/span>[\/vc_column_text][\/vc_column_inner][vc_column_inner width=&#8221;1\/3&#8243; css=&#8221;.vc_custom_1448621169620{margin-top: 20px !important;}&#8221;]<a class=\"btn btn-standard form-btn\"  href=\"https:\/\/www.neb-online.de\/en\/katalog\/\" style=\"margin-left:auto;margin-right:auto;\">Order your free copy<\/a><a class=\"btn btn-standard form-btn\" target=\"_blank\" href=\"http:\/\/www.neb-online.de\/literatur\/pdf\/Isothermal_DNA_Amplification.pdf\" style=\"margin-left:auto;margin-right:auto;\">Download PDF<\/a>[\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row bwd_show_when_type=&#8221;before&#8221; bwd_show_when_date=&#8221;12\/31\/2023 23:00&#8243; bwd_tbc4vd_show_weekday=&#8221;&#8221;][vc_column][vc_separator color=&#8221;black&#8221;][vc_column_text]<\/p>\n<h3>Available Products<\/h3>\n<p>[\/vc_column_text][vc_column_text]<\/p>\n<h2>Product table<\/h2>\n<table style=\"width: 760px;\" border=\"0\" cellpadding=\"5\">\n<tbody>\n<tr style=\"background-color: #f3f3f3; font-size: 16px;\">\n<th style=\"border-bottom: solid #eb7e2d;\" align=\"left\" width=\"400\" height=\"40px\">PRODUCT<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"88\">Prod. #<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"100\">SIZE<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" colspan=\"3\">PRICE<\/th>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\">Bst 2.0 DNA Polymerase<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0537S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">1.600 units<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>79 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/M0537\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/m0537\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0537L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">8.000 units<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>316 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/M0537\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/m0537\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Bst 2.0 DNA Polymerase, conc.<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0537M<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">8.000 units<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>316 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/M0537\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/m0537\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\">Bst 2.0 Warm Start DNA Polymerase<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0538S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">1.600 units<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>88 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/M0538\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/m0538\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0538L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">8.000 units<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>347 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/M0538\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/m0538\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Bst 2.0 Warm Start DNA Polymerase, conc.<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0538M<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">8.000 units<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>347 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/M0538\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/m0538\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\">Bst 3.0 DNA Polymerase<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0374S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">1.600 units<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>78 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/M0374\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/m0374\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0374L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">8.000 units<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>311 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/M0374\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/m0374\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Bst 3.0 DNA Polymerase, conc.<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0374M<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">8.000 units<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>311 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/M0374\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/m0374\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Isothermal Amplification Buffer<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">B0537S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">6.0 ml<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>32<\/strong><strong> \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/B0537\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/b0537\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"padding-top: 50px;\" colspan=\"6\"><strong>Passend dazu:<\/strong><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\">Deoxynucleotide Solutions, Mix<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">N0447S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">8 \u00b5mol of each<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>68 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/N0447\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/n0447\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">N0447L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">40 \u00b5mol of each<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>271 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/N0447\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/n0447\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p style=\"text-align: right;\"><em><span class=\"bildunterschrift\">As of: 01.01.2023<\/span><\/em><\/p>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row bwd_show_when_type=&#8221;after&#8221; bwd_show_when_date=&#8221;12\/31\/2023 23:00&#8243; bwd_tbc4vd_show_weekday=&#8221;&#8221;][vc_column][vc_separator color=&#8221;black&#8221;][vc_column_text]<\/p>\n<h3>Available Products<\/h3>\n<p>[\/vc_column_text][vc_column_text]<\/p>\n<h2>Product table<\/h2>\n<table style=\"width: 760px;\" border=\"0\" cellpadding=\"5\">\n<tbody>\n<tr style=\"background-color: #f3f3f3; font-size: 16px;\">\n<th style=\"border-bottom: solid #eb7e2d;\" align=\"left\" width=\"400\" height=\"40px\">PRODUCT<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"88\">Prod. #<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"100\">SIZE<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" colspan=\"2\">INFO<\/th>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\">Bst 2.0 DNA Polymerase<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0537S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">1.600 units<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/M0537\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/m0537\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0537L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">8.000 units<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/M0537\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/m0537\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Bst 2.0 DNA Polymerase, conc.<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0537M<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">8.000 units<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/M0537\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/m0537\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\">Bst 2.0 Warm Start DNA Polymerase<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0538S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">1.600 units<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/M0538\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/m0538\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0538L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">8.000 units<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/M0538\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/m0538\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Bst 2.0 Warm Start DNA Polymerase, conc.<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0538M<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">8.000 units<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/M0538\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/m0538\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\">Bst 3.0 DNA Polymerase<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0374S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">1.600 units<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/M0374\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/m0374\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0374L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">8.000 units<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/M0374\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/m0374\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Bst 3.0 DNA Polymerase, conc.<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M0374M<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">8.000 units<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/M0374\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/m0374\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Isothermal Amplification Buffer<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">B0537S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">6.0 ml<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/B0537\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/b0537\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"padding-top: 50px;\" colspan=\"6\"><strong>Passend dazu:<\/strong><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\">Deoxynucleotide Solutions, Mix<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">N0447S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">8 \u00b5mol of each<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/N0447\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/n0447\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">N0447L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">40 \u00b5mol of each<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/N0447\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/n0447\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p style=\"text-align: right;\"><em><span class=\"bildunterschrift\">As of: 01.01.2024<\/span><\/em><\/p>\n<p>[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row][vc_column][vc_basic_grid post_type=&#8221;post&#8221; max_items=&#8221;10&#8243; element_width=&#8221;12&#8243; gap=&#8221;15&#8243; item=&#8221;4123&#8243; taxonomies=&#8221;70&#8243; grid_id=&#8221;vc_gid:1702997108643-121d7718-780c-9&#8243;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;2\/3&#8243;][vc_column_text] Bst 2.0 DNA Polymerase Bst\u00a02.0 DNA Polymerase is an\u00a0in silico\u00a0designed homologue of\u00a0Bacillus stearothermophilus\u00a0DNA Polymerase I, Large Fragment (Bst\u00a0DNA Polymerase, Large Fragment).\u00a0Bst\u00a02.0 DNA Polymerase contains 5\u00b4\u21923\u00b4 DNA polymerase activity and strong strand displacement activity but lacks 5\u00b4\u21923\u00b4 exonuclease activity.\u00a0Bst\u00a02.0 DNA Polymerase displays improved amplification speed, yield, salt&#8230;  <a class=\"excerpt-read-more\" href=\"https:\/\/www.neb-online.de\/en\/pcr-and-dna-amplification\/isothermal-amplification-polymerases\/\" title=\"Read Isothermal Amplification \u2013 Polymerases\">Read more &raquo;<\/a><\/p>\n","protected":false},"author":18,"featured_media":39392,"parent":2092,"menu_order":0,"comment_status":"open","ping_status":"closed","template":"page-sidebar.php","meta":{"footnotes":""},"class_list":["post-10346","page","type-page","status-publish","has-post-thumbnail","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.1.1 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Isothermal Amplification \u2013 Polymerases - New England Biolabs GmbH<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.neb-online.de\/en\/pcr-and-dna-amplification\/isothermal-amplification-polymerases\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Isothermal Amplification \u2013 Polymerases - New England Biolabs GmbH\" \/>\n<meta property=\"og:description\" content=\"[vc_row][vc_column][vc_basic_grid post_type=&#8221;post&#8221; max_items=&#8221;10&#8243; element_width=&#8221;12&#8243; gap=&#8221;15&#8243; item=&#8221;4123&#8243; taxonomies=&#8221;70&#8243; grid_id=&#8221;vc_gid:1702997108643-121d7718-780c-9&#8243;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;2\/3&#8243;][vc_column_text] Bst 2.0 DNA Polymerase Bst\u00a02.0 DNA Polymerase is an\u00a0in silico\u00a0designed homologue of\u00a0Bacillus stearothermophilus\u00a0DNA Polymerase I, Large Fragment (Bst\u00a0DNA Polymerase, Large Fragment).\u00a0Bst\u00a02.0 DNA Polymerase contains 5\u00b4\u21923\u00b4 DNA polymerase activity and strong strand displacement activity but lacks 5\u00b4\u21923\u00b4 exonuclease activity.\u00a0Bst\u00a02.0 DNA Polymerase displays improved amplification speed, yield, salt... 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