{"id":10787,"date":"2016-12-12T08:48:56","date_gmt":"2016-12-12T07:48:56","guid":{"rendered":"http:\/\/www.neb-online.de\/en\/neb\/luna-universal-qpcr-rt-qpcr-experience-best-in-class-performance\/"},"modified":"2020-09-09T15:35:02","modified_gmt":"2020-09-09T13:35:02","slug":"luna-universal-qpcr-rt-qpcr-experience-best-in-class-performance","status":"publish","type":"page","link":"https:\/\/www.neb-online.de\/en\/luna-universal-qpcr-rt-qpcr-experience-best-in-class-performance\/","title":{"rendered":"Luna<sup>\u00ae<\/sup> Universal qPCR &#038; RT-qPCR \u2013 Experience best-in-class performance"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row bwd_tbc4vd_show_weekday=&#8221;&#8221; css=&#8221;.vc_custom_1599658497152{margin-bottom: 20px !important;}&#8221;][vc_column][vc_basic_grid post_type=&#8221;post&#8221; max_items=&#8221;3&#8243; element_width=&#8221;12&#8243; item=&#8221;4123&#8243; grid_id=&#8221;vc_gid:1599658391309-1254ee07-dba1-1&#8243; taxonomies=&#8221;74, 164&#8243;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]All NEB products undergo rigorous testing to ensure optimal performance, and Luna is no exception. We took into consideration numerous important traits when evaluating qPCR, including specificity, sensitivity, accuracy and reproducibility, to develop best-in-class qPCR products. Furthermore, we did a comprehensive evaluation of many commercially-available qPCR and RT-qPCR products, and developed a method of analysis that allows you to quickly compare and evaluate the performance of these products. We wanted to be sure that Luna products will perform to your expectations for all of your targets.[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<\/p>\n<div class=\"two-up\">\n<div class=\"layout-component\">\n<h3>Benefit from rigorous testing<\/h3>\n<ul>\n<li>All Luna reagents were evaluated for specificity, sensitivity, efficiency, accuracy, linearity and reproducibility, and optimized for performance<\/li>\n<li>Development of novel &#8220;dots in boxes&#8221; analysis method for concise visual presentation of comprehensive evaluations<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<p>[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<\/p>\n<h3>Use with ALL your targets<\/h3>\n<ul>\n<li>Extensive panel testing and evaluation ensures reproducible results<\/li>\n<li>Luna reagents are optimized for strong performance across a wide range of targets and templates, including those of varied length, GC-content and abundance<\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][vc_column_text]<\/p>\n<h2>Videos<\/h2>\n<p>[\/vc_column_text][vc_row_inner][vc_column_inner width=&#8221;1\/2&#8243;][vc_column_text]<span class=\"bildunterschrift\"><a href=\"https:\/\/secured.benchfly.com\/player\/1479913429624-pm1ssknE4v\/\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-10715\" src=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2016\/12\/Video_Thumb_DotsInBoxes_LUNA-qPCR.jpg\" alt=\"LUNA qPCR Video\" width=\"300\" height=\"192\" \/><\/a>Learn how we analyzed plates and plates of qPCR data, and how LUNA stacks up to the competition.<\/span>[\/vc_column_text][\/vc_column_inner][vc_column_inner width=&#8221;1\/2&#8243;][vc_column_text]<span class=\"bildunterschrift\"><a href=\"https:\/\/secured.benchfly.com\/player\/1479912631215-fZf1lMzWH7\/\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-10716\" src=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2016\/12\/Video_Thumb_OverviewOf_qPCR.jpg\" alt=\"LUNA qPCR\" width=\"300\" height=\"192\" \/><\/a>Learn the basics of qPCR in this short animation.<\/span>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][vc_column_text]<\/p>\n<div class=\"layout-component divider\">\n<h2>Evaluating qPCR results: capturing performance as &#8220;dots in boxes&#8221;<\/h2>\n<p>NEB has developed a method to better evaluate the large amount of qPCR data generated in an experiment. The output of this analysis is known as a dot plot, and captures the key features of a successful, high-quality qPCR experiment as a single point. This method of analysis allows many targets and conditions to be compared in a single graph.<\/p>\n<p>For each experiment, triplicate reactions are set up across a five-log range of input template concentrations (Amplification plot, bottom-left). Three non-template control (NTC) reactions are also included, for a total of 18 reactions per condition\/target. Efficiency (%) is calculated (Standard\u00a0curve,\u00a0top-left) and is plotted against \u0394C<sub>q<\/sub> (dot plot, center), which is the difference between\u00a0the average C<sub>q<\/sub> of the NTC and the lowest input. This parameter captures both detection of the lowest input and non-template amplification. Acceptable performance criteria are defined as an Efficiency of 90-110% and a \u0394C<sub>q<\/sub> of \u2265 3 (green box).<\/p>\n<p>Other performance criteria are captured using a 5-point Quality Score (top-right). Included are:<\/p>\n<ol>\n<li>1. Linearity of amplification, as indicated by the R<sup>2<\/sup> standard curve<\/li>\n<li>2. Reproducibility, as indicated by the consistency of triplicate C<sub>q<\/sub> values for each input concentration<\/li>\n<li>3. Fluorescence consistency, as indicated by similar endpoint fluorescence (RFU<sub>max<\/sub>)<\/li>\n<li>4. Curve steepness<\/li>\n<li>5. Sigmoid curve shape<\/li>\n<\/ol>\n<p>Quality Score is represented by the size and fill of the plotted dot, with experiments that pass all performance criteria represented by a solid dot within the box. These scoring methods were built upon the MIQE qPCR\/RT-qPCR guidelines (Bustin, S.A. et al. (2009)<em> Clin Chem<\/em>. 55, 611-22 and Trombley Hall, A. et al. (2013)<em> PLoS One<\/em> 8(9):e73845). An example of this analysis is shown below.<\/p>\n<\/div>\n<p>[\/vc_column_text][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n<h2>Breaking it down: how we translate qPCR data into &#8220;dots in boxes&#8221;<\/h2>\n<p>[\/vc_column_text][vc_single_image image=&#8221;11237&#8243; img_size=&#8221;full&#8221; onclick=&#8221;img_link_large&#8221;][vc_separator][vc_column_text]<\/p>\n<h2>See how Luna products stack up<\/h2>\n<p>Comprehensive market-wide evaluation of qPCR and RT-qPCR products reveals that Luna outperforms the competition.<\/p>\n<p><strong>Evaluation of commercially-available qPCR products demonstrates robustness and specificity of Luna<\/strong>[\/vc_column_text][vc_single_image image=&#8221;10882&#8243; img_size=&#8221;full&#8221; img_link_large=&#8221;yes&#8221;][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][vc_column_text]<\/p>\n<h2>Product table<\/h2>\n<table style=\"width: 760px;\" border=\"0\" cellpadding=\"5\">\n<tbody>\n<tr style=\"background-color: #f3f3f3; font-size: 16px;\">\n<th style=\"border-bottom: solid #eb7e2d;\" align=\"left\" width=\"400\" height=\"40px\">PRODUCT<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"88\">Prod. #<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"100\">SIZE<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" colspan=\"3\">PRICE<\/th>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"4\">Luna<sup>\u00ae<\/sup> Universal qPCR Master Mix<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M3003S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">200 rxns (2x1ml)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>105 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=M3003\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/products\/m3003-luna-universal-qpcr-master-mix\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M3003L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">500 rxns (5x1ml)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>237 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=M3003\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/products\/m3003-luna-universal-qpcr-master-mix\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M3003X<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">1000 rxns (10x1ml)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>424 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=M3003\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/products\/m3003-luna-universal-qpcr-master-mix\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M3003E<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">2500 rxns (1x25ml)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>948 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=M3003\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/products\/m3003-luna-universal-qpcr-master-mix\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"4\">Luna<sup>\u00ae<\/sup> Universal Probe qPCR Master Mix<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M3004S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">200 rxns (2x1ml)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>88 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=M3004\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/products\/m3004-luna-universal-probe-qpcr-master-mix\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M3004L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">500 rxns (5x1ml)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>198 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=M3004\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/products\/m3004-luna-universal-probe-qpcr-master-mix\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M3004X<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">1000 rxns (10x1ml)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>364 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=M3004\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/products\/m3004-luna-universal-probe-qpcr-master-mix\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M3004E<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">2500 rxns (1x25ml)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>812 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=M3004\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/products\/m3004-luna-universal-probe-qpcr-master-mix\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"4\">Luna<sup>\u00ae<\/sup> Universal One-Step RT-qPCR Kit<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E3005S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">200 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>218 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=E3005\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/products\/e3005-luna-universal-one-step-rt-qpcr-kit\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E3005L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">500 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>498 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=E3005\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/products\/e3005-luna-universal-one-step-rt-qpcr-kit\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E3005X<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">1000 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>869 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=E3005\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/products\/e3005-luna-universal-one-step-rt-qpcr-kit\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E3005E<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">2500 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>1.924 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=E3005\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/products\/e3005-luna-universal-one-step-rt-qpcr-kit\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"4\">Luna<sup>\u00ae<\/sup> Universal Probe One-Step RT-qPCR Kit<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E3006S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">200 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>198 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=E3006\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/products\/e3006-luna-universal-probe-one-step-rt-qpcr-kit\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E3006L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">500 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>448 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=E3006\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/products\/e3006-luna-universal-probe-one-step-rt-qpcr-kit\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E3006X<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">1000 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>788 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=E3006\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/products\/e3006-luna-universal-probe-one-step-rt-qpcr-kit\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E3006E<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">2500 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>1.738 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=E3006\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/products\/e3006-luna-universal-probe-one-step-rt-qpcr-kit\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\">LunaScript<sup>\u2122<\/sup> RT SuperMix Kit<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E3010S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">25 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>125 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=E3010\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/products\/e3010-luna-script-rt-super-mix-kit#Product%20Information\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E3010L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>420 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=E3010\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/products\/e3010-luna-script-rt-super-mix-kit#Product%20Information\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p style=\"text-align: right;\"><span class=\"bildunterschrift\">As of: 03.01.2019<\/span><\/p>\n<p>[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row bwd_tbc4vd_show_weekday=&#8221;&#8221; css=&#8221;.vc_custom_1599658497152{margin-bottom: 20px !important;}&#8221;][vc_column][vc_basic_grid post_type=&#8221;post&#8221; max_items=&#8221;3&#8243; element_width=&#8221;12&#8243; item=&#8221;4123&#8243; grid_id=&#8221;vc_gid:1599658391309-1254ee07-dba1-1&#8243; taxonomies=&#8221;74, 164&#8243;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]All NEB products undergo rigorous testing to ensure optimal performance, and Luna is no exception. We took into consideration numerous important traits when evaluating qPCR, including specificity, sensitivity, accuracy and reproducibility, to develop best-in-class qPCR products. Furthermore, we did a comprehensive evaluation of many&#8230;  <a class=\"excerpt-read-more\" href=\"https:\/\/www.neb-online.de\/en\/luna-universal-qpcr-rt-qpcr-experience-best-in-class-performance\/\" title=\"Read Luna&lt;sup&gt;\u00ae&lt;\/sup&gt; Universal qPCR &#038; RT-qPCR \u2013 Experience best-in-class performance\">Read more &raquo;<\/a><\/p>\n","protected":false},"author":18,"featured_media":14701,"parent":0,"menu_order":0,"comment_status":"open","ping_status":"closed","template":"page-sidebar.php","meta":{"footnotes":""},"class_list":["post-10787","page","type-page","status-publish","has-post-thumbnail","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.1.1 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Luna\u00ae Universal qPCR &amp; RT-qPCR \u2013 Experience best-in-class performance - New England Biolabs GmbH<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.neb-online.de\/en\/luna-universal-qpcr-rt-qpcr-experience-best-in-class-performance\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Luna\u00ae Universal qPCR &amp; RT-qPCR \u2013 Experience best-in-class performance - New England Biolabs GmbH\" \/>\n<meta property=\"og:description\" content=\"[vc_row bwd_tbc4vd_show_weekday=&#8221;&#8221; css=&#8221;.vc_custom_1599658497152{margin-bottom: 20px !important;}&#8221;][vc_column][vc_basic_grid post_type=&#8221;post&#8221; max_items=&#8221;3&#8243; element_width=&#8221;12&#8243; item=&#8221;4123&#8243; grid_id=&#8221;vc_gid:1599658391309-1254ee07-dba1-1&#8243; taxonomies=&#8221;74, 164&#8243;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]All NEB products undergo rigorous testing to ensure optimal performance, and Luna is no exception. We took into consideration numerous important traits when evaluating qPCR, including specificity, sensitivity, accuracy and reproducibility, to develop best-in-class qPCR products. Furthermore, we did a comprehensive evaluation of many... 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