{"id":13991,"date":"2018-01-02T16:46:55","date_gmt":"2018-01-02T15:46:55","guid":{"rendered":"http:\/\/www.neb-online.de\/en\/genome-editing\/sgrna-synthese\/"},"modified":"2023-12-19T14:33:51","modified_gmt":"2023-12-19T13:33:51","slug":"sgrna-synthesis","status":"publish","type":"page","link":"https:\/\/www.neb-online.de\/en\/genome-editing\/sgrna-synthesis\/","title":{"rendered":"sgRNA Synthesis"},"content":{"rendered":"

[vc_row][vc_column][vc_basic_grid post_type=”post” max_items=”3″ element_width=”12″ item=”4123″ grid_id=”vc_gid:1702992784329-44b97e25-8e46-3″ taxonomies=”145, 69, 70″][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]Along with Cas9 nuclease, CRISPR experiments require the introduction of an sgRNA containing an approximately 20-base sequence specific to the target DNA 5\u2032 of a non-variable scaffold sequence. sgRNA can be delivered as RNA or by transforming with a plasmid with the sgRNA-coding sequence under a promoter. A number of strategies have been developed to quickly swap out the 20 base sequences allowing convenient sgRNA cloning using NEB products.[\/vc_column_text][vc_single_image image=”9639″ img_size=”full” onclick=”link_image”][vc_column_text]Plasmids containing sgRNA sequences can be constructed using a variety of methods. Common to each is the requirement to introduce an approximately 20 base target sequence downstream of a promoter. Guide RNA templates can then be used as templates for in vitro transcription or directly introduced.<\/span>[\/vc_column_text][vc_single_image image=”13983″ img_size=”full” onclick=”link_image”][\/vc_column][\/vc_row][vc_row bwd_show_when_type=”before” bwd_show_when_date=”12\/31\/2023 23:00″ bwd_tbc4vd_show_weekday=””][vc_column][vc_separator color=”black”][vc_column_text]<\/p>\n

Product table<\/h2>\n\n\n\n\n\n\n\n\n\n\n\n
PRODUCT<\/th>\nProd. #<\/th>\nSIZE<\/th>\nPRICE<\/th>\n<\/tr>\n
EnGen\u00ae<\/sup> sgRNA Synthesis Kit S. pyogenes<\/em><\/td>\nE3322S<\/td>\n20 rxns<\/td>\n448 \u20ac<\/strong><\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
E3322V<\/td>\n10 rxns<\/td>\n225 \u20ac<\/strong><\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
HiScribe\u2122 T7 High Yield RNA Synthesis Kit<\/td>\nE2040S<\/td>\n50 rxns<\/td>\n247 \u20ac<\/strong><\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
HiScribe\u2122 T7 Quick High Yield RNA Synthesis Kit<\/td>\nE2050S<\/td>\n50 rxns<\/td>\n302 \u20ac<\/strong><\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
Q5\u00ae<\/sup> High-Fidelity DNA Polymerase<\/td>\nM0491S<\/td>\n100 units<\/td>\n108 \u20ac<\/strong><\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
M0491L<\/td>\n500 units<\/td>\n474 \u20ac<\/strong><\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
Q5\u00ae<\/sup> Hot Start High-Fidelity DNA Polymerase<\/td>\nM0493S<\/td>\n100 units<\/td>\n126 \u20ac<\/strong><\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
M0493L<\/td>\n500 units<\/td>\n570 \u20ac<\/strong><\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

As of: 01.01.2023<\/span><\/p>\n

[\/vc_column_text][\/vc_column][\/vc_row][vc_row bwd_show_when_type=”after” bwd_show_when_date=”12\/31\/2023 23:00″ bwd_tbc4vd_show_weekday=””][vc_column][vc_separator color=”black”][vc_column_text]<\/p>\n

Product table<\/h2>\n\n\n\n\n\n\n\n\n\n\n\n
PRODUCT<\/th>\nProd. #<\/th>\nSIZE<\/th>\nINFO<\/th>\n<\/tr>\n
EnGen\u00ae<\/sup> sgRNA Synthesis Kit S. pyogenes<\/em><\/td>\nE3322S<\/td>\n20 rxns<\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
E3322V<\/td>\n10 rxns<\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
HiScribe\u2122 T7 High Yield RNA Synthesis Kit<\/td>\nE2040S<\/td>\n50 rxns<\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
HiScribe\u2122 T7 Quick High Yield RNA Synthesis Kit<\/td>\nE2050S<\/td>\n50 rxns<\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
Q5\u00ae<\/sup> High-Fidelity DNA Polymerase<\/td>\nM0491S<\/td>\n100 units<\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
M0491L<\/td>\n500 units<\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
Q5\u00ae<\/sup> Hot Start High-Fidelity DNA Polymerase<\/td>\nM0493S<\/td>\n100 units<\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
M0493L<\/td>\n500 units<\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

As of: 01.01.2024<\/span><\/p>\n

[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"

[vc_row][vc_column][vc_basic_grid post_type=”post” max_items=”3″ element_width=”12″ item=”4123″ grid_id=”vc_gid:1702992784329-44b97e25-8e46-3″ taxonomies=”145, 69, 70″][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]Along with Cas9 nuclease, CRISPR experiments require the introduction of an sgRNA containing an approximately 20-base sequence specific to the target DNA 5\u2032 of a non-variable scaffold sequence. sgRNA can be delivered as RNA or by transforming with a plasmid with the sgRNA-coding sequence under a promoter…. Read more »<\/a><\/p>\n","protected":false},"author":18,"featured_media":41240,"parent":2072,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-sidebar.php","meta":{"footnotes":""},"class_list":["post-13991","page","type-page","status-publish","has-post-thumbnail","hentry"],"yoast_head":"\nsgRNA Synthesis - New England Biolabs GmbH<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.neb-online.de\/en\/genome-editing\/sgrna-synthesis\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"sgRNA Synthesis - New England Biolabs GmbH\" \/>\n<meta property=\"og:description\" content=\"[vc_row][vc_column][vc_basic_grid post_type=”post” max_items=”3″ element_width=”12″ item=”4123″ grid_id=”vc_gid:1702992784329-44b97e25-8e46-3″ taxonomies=”145, 69, 70″][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]Along with Cas9 nuclease, CRISPR experiments require the introduction of an sgRNA containing an approximately 20-base sequence specific to the target DNA 5\u2032 of a non-variable scaffold sequence. sgRNA can be delivered as RNA or by transforming with a plasmid with the sgRNA-coding sequence under a promoter.... 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