{"id":13993,"date":"2018-01-02T16:52:02","date_gmt":"2018-01-02T15:52:02","guid":{"rendered":"http:\/\/www.neb-online.de\/en\/genome-editing\/engen-mutation-detection-kit\/"},"modified":"2023-12-19T14:35:17","modified_gmt":"2023-12-19T13:35:17","slug":"engen-mutation-detection-kit","status":"publish","type":"page","link":"https:\/\/www.neb-online.de\/en\/genome-editing\/engen-mutation-detection-kit\/","title":{"rendered":"EnGen Mutation Detection Kit"},"content":{"rendered":"

[vc_row][vc_column][vc_basic_grid post_type=”post” max_items=”3″ element_width=”12″ item=”4123″ grid_id=”vc_gid:1702992883444-db4da7b7-10c3-10″ taxonomies=”69, 70, 145″][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n

Measuring Targeting Efficiency<\/h2>\n

[\/vc_column_text][vc_row_inner][vc_column_inner width=”1\/2″][vc_column_text]A widely used method to identify mutations is the T7 Endonuclease I mutation detection assay (1,2). This assay detects heteroduplex DNA that results from the annealing of a DNA strand, including desired mutations, with a wild-type DNA strand. The EnGen Mutation Detection Kit provides optimized reagents for performing robust T7 Endonuclease-based detection of genome editing events.<\/p>\n

Reyon, D. et al. (2012) Nat. Biotechnol., 30(5):460-5
\n<\/span>Vouillot, L., et al. (2015) G3 (Bethesda), 5(3):407-15<\/span>[\/vc_column_text][\/vc_column_inner][vc_column_inner width=”1\/2″][vc_single_image image=”8840″ img_size=”medium” alignment=”center” onclick=”link_image”][vc_column_text]Genomic DNA is amplified with primers bracketing the modified locus. PCR products are then denatured and re-annealed yielding three classes of possible structures. Duplexes containing a mismatch greater than one base are digested by T7 Endonuclease I. The DNA is then electrophoretically separated and fragment analysis is used to estimate targeting efficiency.<\/span>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_separator color=”black”][\/vc_column][\/vc_row][vc_row bwd_show_when_type=”before” bwd_show_when_date=”12\/31\/2023 23:00″ bwd_tbc4vd_show_weekday=””][vc_column][vc_column_text]<\/p>\n

Product table<\/h2>\n\n\n\n\n
PRODUCT<\/th>\nProd. #<\/th>\nSIZE<\/th>\nPRICE<\/th>\n<\/tr>\n
EnGen\u00ae<\/sup> Mutation Detection Kit<\/td>\nE3321S<\/td>\n25 rxns<\/td>\n228 \u20ac<\/strong><\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Stand: 01.01.2023<\/span><\/em><\/p>\n

[\/vc_column_text][\/vc_column][\/vc_row][vc_row bwd_show_when_type=”after” bwd_show_when_date=”12\/31\/2023 23:00″ bwd_tbc4vd_show_weekday=””][vc_column][vc_column_text]<\/p>\n

Product table<\/h2>\n\n\n\n\n
PRODUCT<\/th>\nProd. #<\/th>\nSIZE<\/th>\nINFO<\/th>\n<\/tr>\n
EnGen\u00ae<\/sup> Mutation Detection Kit<\/td>\nE3321S<\/td>\n25 rxns<\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Stand: 01.01.2024<\/span><\/em><\/p>\n

[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"

[vc_row][vc_column][vc_basic_grid post_type=”post” max_items=”3″ element_width=”12″ item=”4123″ grid_id=”vc_gid:1702992883444-db4da7b7-10c3-10″ taxonomies=”69, 70, 145″][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text] Measuring Targeting Efficiency [\/vc_column_text][vc_row_inner][vc_column_inner width=”1\/2″][vc_column_text]A widely used method to identify mutations is the T7 Endonuclease I mutation detection assay (1,2). This assay detects heteroduplex DNA that results from the annealing of a DNA strand, including desired mutations, with a wild-type DNA strand. The EnGen Mutation Detection… Read more »<\/a><\/p>\n","protected":false},"author":18,"featured_media":41240,"parent":2072,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-sidebar.php","meta":{"footnotes":""},"class_list":["post-13993","page","type-page","status-publish","has-post-thumbnail","hentry"],"yoast_head":"\nEnGen Mutation Detection Kit - New England Biolabs GmbH<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.neb-online.de\/en\/genome-editing\/engen-mutation-detection-kit\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"EnGen Mutation Detection Kit - New England Biolabs GmbH\" \/>\n<meta property=\"og:description\" content=\"[vc_row][vc_column][vc_basic_grid post_type=”post” max_items=”3″ element_width=”12″ item=”4123″ grid_id=”vc_gid:1702992883444-db4da7b7-10c3-10″ taxonomies=”69, 70, 145″][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text] Measuring Targeting Efficiency [\/vc_column_text][vc_row_inner][vc_column_inner width=”1\/2″][vc_column_text]A widely used method to identify mutations is the T7 Endonuclease I mutation detection assay (1,2). This assay detects heteroduplex DNA that results from the annealing of a DNA strand, including desired mutations, with a wild-type DNA strand. The EnGen Mutation Detection... Read more »\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.neb-online.de\/en\/genome-editing\/engen-mutation-detection-kit\/\" \/>\n<meta property=\"og:site_name\" content=\"New England Biolabs GmbH\" \/>\n<meta property=\"article:modified_time\" content=\"2023-12-19T13:35:17+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/04\/NEB_Banner_GenomeEditing_new_760x200px.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"760\" \/>\n\t<meta property=\"og:image:height\" content=\"200\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.neb-online.de\/en\/genome-editing\/engen-mutation-detection-kit\/\",\"url\":\"https:\/\/www.neb-online.de\/en\/genome-editing\/engen-mutation-detection-kit\/\",\"name\":\"EnGen Mutation Detection Kit - New England Biolabs GmbH\",\"isPartOf\":{\"@id\":\"https:\/\/www.neb-online.de\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/www.neb-online.de\/en\/genome-editing\/engen-mutation-detection-kit\/#primaryimage\"},\"image\":{\"@id\":\"https:\/\/www.neb-online.de\/en\/genome-editing\/engen-mutation-detection-kit\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/04\/NEB_Banner_GenomeEditing_new_760x200px.jpg\",\"datePublished\":\"2018-01-02T15:52:02+00:00\",\"dateModified\":\"2023-12-19T13:35:17+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/www.neb-online.de\/en\/genome-editing\/engen-mutation-detection-kit\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/www.neb-online.de\/en\/genome-editing\/engen-mutation-detection-kit\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/www.neb-online.de\/en\/genome-editing\/engen-mutation-detection-kit\/#primaryimage\",\"url\":\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/04\/NEB_Banner_GenomeEditing_new_760x200px.jpg\",\"contentUrl\":\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/04\/NEB_Banner_GenomeEditing_new_760x200px.jpg\",\"width\":760,\"height\":200},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/www.neb-online.de\/en\/genome-editing\/engen-mutation-detection-kit\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/www.neb-online.de\/en\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Genome Editing\",\"item\":\"https:\/\/www.neb-online.de\/en\/genome-editing\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"EnGen Mutation Detection Kit\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/www.neb-online.de\/#website\",\"url\":\"https:\/\/www.neb-online.de\/\",\"name\":\"New England Biolabs GmbH\",\"description\":\"Enabling Technologies in the Life Sciences\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/www.neb-online.de\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"EnGen Mutation Detection Kit - New England Biolabs GmbH","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.neb-online.de\/en\/genome-editing\/engen-mutation-detection-kit\/","og_locale":"en_US","og_type":"article","og_title":"EnGen Mutation Detection Kit - New England Biolabs GmbH","og_description":"[vc_row][vc_column][vc_basic_grid post_type=”post” max_items=”3″ element_width=”12″ item=”4123″ grid_id=”vc_gid:1702992883444-db4da7b7-10c3-10″ taxonomies=”69, 70, 145″][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text] Measuring Targeting Efficiency [\/vc_column_text][vc_row_inner][vc_column_inner width=”1\/2″][vc_column_text]A widely used method to identify mutations is the T7 Endonuclease I mutation detection assay (1,2). This assay detects heteroduplex DNA that results from the annealing of a DNA strand, including desired mutations, with a wild-type DNA strand. The EnGen Mutation Detection... Read more »","og_url":"https:\/\/www.neb-online.de\/en\/genome-editing\/engen-mutation-detection-kit\/","og_site_name":"New England Biolabs GmbH","article_modified_time":"2023-12-19T13:35:17+00:00","og_image":[{"width":760,"height":200,"url":"https:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/04\/NEB_Banner_GenomeEditing_new_760x200px.jpg","type":"image\/jpeg"}],"twitter_misc":{"Est. reading time":"2 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/www.neb-online.de\/en\/genome-editing\/engen-mutation-detection-kit\/","url":"https:\/\/www.neb-online.de\/en\/genome-editing\/engen-mutation-detection-kit\/","name":"EnGen Mutation Detection Kit - New England Biolabs GmbH","isPartOf":{"@id":"https:\/\/www.neb-online.de\/#website"},"primaryImageOfPage":{"@id":"https:\/\/www.neb-online.de\/en\/genome-editing\/engen-mutation-detection-kit\/#primaryimage"},"image":{"@id":"https:\/\/www.neb-online.de\/en\/genome-editing\/engen-mutation-detection-kit\/#primaryimage"},"thumbnailUrl":"https:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/04\/NEB_Banner_GenomeEditing_new_760x200px.jpg","datePublished":"2018-01-02T15:52:02+00:00","dateModified":"2023-12-19T13:35:17+00:00","breadcrumb":{"@id":"https:\/\/www.neb-online.de\/en\/genome-editing\/engen-mutation-detection-kit\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.neb-online.de\/en\/genome-editing\/engen-mutation-detection-kit\/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.neb-online.de\/en\/genome-editing\/engen-mutation-detection-kit\/#primaryimage","url":"https:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/04\/NEB_Banner_GenomeEditing_new_760x200px.jpg","contentUrl":"https:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/04\/NEB_Banner_GenomeEditing_new_760x200px.jpg","width":760,"height":200},{"@type":"BreadcrumbList","@id":"https:\/\/www.neb-online.de\/en\/genome-editing\/engen-mutation-detection-kit\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/www.neb-online.de\/en\/"},{"@type":"ListItem","position":2,"name":"Genome Editing","item":"https:\/\/www.neb-online.de\/en\/genome-editing\/"},{"@type":"ListItem","position":3,"name":"EnGen Mutation Detection Kit"}]},{"@type":"WebSite","@id":"https:\/\/www.neb-online.de\/#website","url":"https:\/\/www.neb-online.de\/","name":"New England Biolabs GmbH","description":"Enabling Technologies in the Life Sciences","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/www.neb-online.de\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"}]}},"_links":{"self":[{"href":"https:\/\/www.neb-online.de\/en\/wp-json\/wp\/v2\/pages\/13993","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.neb-online.de\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.neb-online.de\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.neb-online.de\/en\/wp-json\/wp\/v2\/users\/18"}],"replies":[{"embeddable":true,"href":"https:\/\/www.neb-online.de\/en\/wp-json\/wp\/v2\/comments?post=13993"}],"version-history":[{"count":11,"href":"https:\/\/www.neb-online.de\/en\/wp-json\/wp\/v2\/pages\/13993\/revisions"}],"predecessor-version":[{"id":20954,"href":"https:\/\/www.neb-online.de\/en\/wp-json\/wp\/v2\/pages\/13993\/revisions\/20954"}],"up":[{"embeddable":true,"href":"https:\/\/www.neb-online.de\/en\/wp-json\/wp\/v2\/pages\/2072"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.neb-online.de\/en\/wp-json\/wp\/v2\/media\/41240"}],"wp:attachment":[{"href":"https:\/\/www.neb-online.de\/en\/wp-json\/wp\/v2\/media?parent=13993"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}