{"id":15146,"date":"2018-04-18T14:34:02","date_gmt":"2018-04-18T12:34:02","guid":{"rendered":"https:\/\/www.neb-online.de\/en\/fastcloning-mit-reagenzien-von-neb\/"},"modified":"2022-05-10T13:45:08","modified_gmt":"2022-05-10T11:45:08","slug":"fastcloning-with-reagents-from-neb","status":"publish","type":"page","link":"https:\/\/www.neb-online.de\/en\/cloning-synthetic-biology\/kits\/fastcloning-with-reagents-from-neb\/","title":{"rendered":"FastCloning with reagents from NEB"},"content":{"rendered":"

[vc_row][vc_column][vc_basic_grid post_type=”post” max_items=”10″ element_width=”12″ gap=”15″ item=”4123″ taxonomies=”72″ grid_id=”vc_gid:1652183053307-36101d93-0696-4″][\/vc_column][\/vc_row][vc_row][vc_row][\/vc_row][vc_column][vc_row_inner][vc_column_inner width=”2\/3″][vc_column_text]Accelerate your cloning experiments with reagents from NEB<\/p>\n

While cloning has typically been a time-consuming process in the past, advances in both technologies and reagents have enabled the process to take much less time. Race to NEB for the highest quality products that save valuable time![\/vc_column_text][\/vc_column_inner][vc_column_inner width=”1\/3″][vc_single_image image=”15177″ img_size=”full” style=”vc_box_shadow”][\/vc_column_inner][\/vc_row_inner][vc_separator][vc_column_text]

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<\/div><\/a><\/div><\/div>[\/vc_column_text][vc_column_text]Click on the “vial” icons to learn more!<\/em>[\/vc_column_text][vc_separator][vc_column_text]<\/p>\n

Fast cloning reagents from NEB:<\/h2>\n

[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=”1\/2″][vc_column_text]<\/p>\n

SEAMLESS CLONING<\/h3>\n

[\/vc_column_text][vc_row_inner][vc_column_inner width=”1\/6″][vc_single_image image=”14975″ img_size=”full”][\/vc_column_inner][vc_column_inner width=”5\/6″][vc_column_text]NEBuilder HiFi DNA Assembly Master Mix<\/span><\/strong><\/a>, NEBuilder HiFi DNA Assembly Cloning Kit<\/a><\/span><\/strong> & NEBuilder HiFi DNA Assembly Bundle for Large Fragments<\/strong><\/span><\/a><\/p>\n

Rapid, seamless assembly\/cloning of multiple fragments in as little as 15 minute[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner][vc_column_inner width=”1\/6″][vc_single_image image=”14975″ img_size=”full”][\/vc_column_inner][vc_column_inner width=”5\/6″][vc_column_text]NEBridge Golden Gate Assembly Kit (BsaI-HF\u00aev2)<\/a><\/span><\/strong><\/p>\n

Efficient, ordered assembly of multiple fragments in a single reaction[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_column_text]<\/p>\n

DIGESTION<\/h3>\n

[\/vc_column_text][vc_row_inner][vc_column_inner width=”1\/6″][vc_single_image image=”14975″ img_size=”full”][\/vc_column_inner][vc_column_inner width=”5\/6″][vc_column_text]High-Fidelity (HF) restriction enzymes<\/strong><\/span><\/a><\/p>\n

HF enzymes offer reduced star activity, rapid digestion and 100% activity in CutSmart\u00ae Buffer[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner][vc_column_inner width=”1\/6″][vc_single_image image=”14975″ img_size=”full”][\/vc_column_inner][vc_column_inner width=”5\/6″][vc_column_text]Time-Saver restriction enzymes<\/strong><\/span><\/a><\/p>\n

>195 enzymes are Time-Saver qualified and will digest DNA in 5-15 minutes; digest overnight with no unwanted star activity[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_column_text]<\/p>\n

END MODIFICATION<\/h3>\n

[\/vc_column_text][vc_row_inner][vc_column_inner width=”1\/6″][vc_single_image image=”14975″ img_size=”full”][\/vc_column_inner][vc_column_inner width=”5\/6″][vc_column_text]Quick CIP<\/a><\/span><\/strong><\/p>\n

Complete dephosphorylation in 10 minutes with rapid, irreversible heat inactivation[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner][vc_column_inner width=”1\/6″][vc_single_image image=”14975″ img_size=”full”][\/vc_column_inner][vc_column_inner width=”5\/6″][vc_column_text]Quick Blunting Kit<\/a><\/span><\/strong><\/p>\n

Blunt restriction enzyme-digested DNA in less than 30 minutes at room temperature[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_column][vc_column width=”1\/2″][vc_column_text]<\/p>\n

AMPLIFICATION<\/h3>\n

[\/vc_column_text][vc_row_inner][vc_column_inner width=”1\/6″][vc_single_image image=”14975″ img_size=”full”][\/vc_column_inner][vc_column_inner width=”5\/6″][vc_column_text]Q5 High-Fidelity DNA Polymerases<\/a><\/strong><\/span><\/p>\n

With the lowest error rate of all known commercially available PCR polymerases, Q5 enables extension times as low as 10 seconds per kb on templates up to 20 kb[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_column_text]<\/p>\n

PURIFICATION<\/h3>\n

[\/vc_column_text][vc_row_inner][vc_column_inner width=”1\/6″][vc_single_image image=”14975″ img_size=”full”][\/vc_column_inner][vc_column_inner width=”5\/6″][vc_column_text]Monarch DNA Purification Kits<\/span><\/strong><\/a><\/p>\n

Purify high-quality DNA in minutes (5 minutes for the PCR & DNA Cleanup Kit!). Designed with sustainability in mind, Monarch kits use less plastic and responsibly-sourced packaging[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_column_text]<\/p>\n

LIGATION<\/h3>\n

[\/vc_column_text][vc_row_inner][vc_column_inner width=”1\/6″][vc_single_image image=”14975″ img_size=”full”][\/vc_column_inner][vc_column_inner width=”5\/6″][vc_column_text]Instant Sticky-end Ligase Master Mix<\/a><\/span><\/strong><\/p>\n

Specially formulated to instantly ligate 2-4 basepair overhangs! Just add DNA, flick the tube, and ligation occurs instantly! No need for incubation.[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner][vc_column_inner width=”1\/6″][vc_single_image image=”14975″ img_size=”full”][\/vc_column_inner][vc_column_inner width=”5\/6″][vc_column_text]Blunt\/TA Ligase Master Mix<\/a><\/span><\/strong><\/p>\n

Specially formulated to ligate single-base and blunt ends in just 15 minutes[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner][vc_column_inner width=”1\/6″][vc_single_image image=”14975″ img_size=”full”][\/vc_column_inner][vc_column_inner width=”5\/6″][vc_column_text]Quick Ligation Kit<\/a><\/span><\/strong><\/p>\n

Ligates both cohesive and blunt ends in just 5 minutes at room temperature[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_column_text]<\/p>\n

TRANSFORMATION<\/h3>\n

[\/vc_column_text][vc_row_inner][vc_column_inner width=”1\/6″][vc_single_image image=”14975″ img_size=”full”][\/vc_column_inner][vc_column_inner width=”5\/6″][vc_column_text]NEB Turbo Competent E. coli<\/em> (High-Efficiency)<\/a><\/span><\/strong><\/p>\n

Generate colonies in as little as 6.5 hours with a 5-minute transformation protocol[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"

[vc_row][vc_column][vc_basic_grid post_type=”post” max_items=”10″ element_width=”12″ gap=”15″ item=”4123″ taxonomies=”72″ grid_id=”vc_gid:1652183053307-36101d93-0696-4″][\/vc_column][\/vc_row][vc_row][vc_row][\/vc_row][vc_column][vc_row_inner][vc_column_inner width=”2\/3″][vc_column_text]Accelerate your cloning experiments with reagents from NEB While cloning has typically been a time-consuming process in the past, advances in both technologies and reagents have enabled the process to take much less time. Race to NEB for the highest quality products that save valuable time![\/vc_column_text][\/vc_column_inner][vc_column_inner width=”1\/3″][vc_single_image… Read more »<\/a><\/p>\n","protected":false},"author":18,"featured_media":24805,"parent":2106,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-sidebar.php","meta":{"footnotes":""},"class_list":["post-15146","page","type-page","status-publish","has-post-thumbnail","hentry"],"yoast_head":"\nFastCloning with reagents from NEB - New England Biolabs GmbH<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.neb-online.de\/en\/cloning-synthetic-biology\/kits\/fastcloning-with-reagents-from-neb\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"FastCloning with reagents from NEB - New England Biolabs GmbH\" \/>\n<meta property=\"og:description\" content=\"[vc_row][vc_column][vc_basic_grid post_type=”post” max_items=”10″ element_width=”12″ gap=”15″ item=”4123″ taxonomies=”72″ grid_id=”vc_gid:1652183053307-36101d93-0696-4″][\/vc_column][\/vc_row][vc_row][vc_row][\/vc_row][vc_column][vc_row_inner][vc_column_inner width=”2\/3″][vc_column_text]Accelerate your cloning experiments with reagents from NEB While cloning has typically been a time-consuming process in the past, advances in both technologies and reagents have enabled the process to take much less time. 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