{"id":15760,"date":"2018-08-08T14:46:44","date_gmt":"2018-08-08T12:46:44","guid":{"rendered":"https:\/\/www.neb-online.de\/en\/monarch-nucleic-acid-purification\/monarch-rna-cleanup-kits-aufreinigung-konzentration\/"},"modified":"2025-01-16T16:49:05","modified_gmt":"2025-01-16T15:49:05","slug":"monarch-rna-cleanup-kits","status":"publish","type":"page","link":"https:\/\/www.neb-online.de\/en\/monarch-nucleic-acid-purification-kits-2\/monarch-rna-cleanup-kits\/","title":{"rendered":"Monarch Spin RNA Cleanup Kits"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row css=&#8221;.vc_custom_1533728291740{margin-bottom: 30px !important;}&#8221;][vc_column][vc_basic_grid post_type=&#8221;post&#8221; max_items=&#8221;3&#8243; element_width=&#8221;12&#8243; item=&#8221;4123&#8243; grid_id=&#8221;vc_gid:1737042147429-dcd053e7-1423-6&#8243; taxonomies=&#8221;138, 164&#8243;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<\/p>\n<h2>Monarch Kits for RNA Purification and Concentration<\/h2>\n<p>The Monarch Spin RNA Cleanup Kits provide a fast and simple silica spin column-based solution for RNA purification and concentration after any enzymatic reaction (including <em>in vitro<\/em> transcription, DNase I treatment, capping and labeling) and after other isolation methods such as phenol\/chloroform extraction.[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_text_separator title=&#8221;Video: RNA Purification Protocol&#8221; color=&#8221;custom&#8221; accent_color=&#8221;#eb7e2d&#8221;][vc_video link=&#8221;https:\/\/www.youtube.com\/watch?v=1BHZMT_p18o&#8221;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<strong>The Monarch Spin RNA Cleanup Kits are available in 3 different binding capacities:<\/strong><\/p>\n<ul>\n<li>for 10 \u03bcg (<a href=\"https:\/\/www.neb.com\/en\/products\/t2030-monarch-rna-cleanup-kit-10-ug#Product%20Information\" target=\"_blank\" rel=\"noopener noreferrer\">NEB #T2030<\/a>)<\/li>\n<li>for 50 \u03bcg (<a href=\"https:\/\/www.neb.com\/en\/products\/t2040-monarch-rna-cleanup-kit-50-ug#Product%20Information\" target=\"_blank\" rel=\"noopener noreferrer\">NEB #T2040<\/a>)<\/li>\n<li>and for 500 \u03bcg (<a href=\"https:\/\/www.neb.com\/en\/products\/t2050-monarch-rna-cleanup-kit-500-ug#Product%20Information\" target=\"_blank\" rel=\"noopener noreferrer\">NEB #T2050<\/a>).<\/li>\n<\/ul>\n<p>Each kit contains unique columns, all designed to prevent buffer retention and ensure no carryover of contaminants, enabling low-volume elution of highly-pure RNA. Following the standard protocol, RNA \u2265 25 nt is purified with this kit; however, a modified protocol is available to enable the binding of RNA as small as 15 nt (including miRNAs).[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_column_text css=&#8221;.vc_custom_1533798958510{border-top-width: 2px !important;border-bottom-width: 2px !important;padding-top: 10px !important;padding-right: 10px !important;padding-bottom: 10px !important;padding-left: 10px !important;background-color: #f4f1e7 !important;border-top-color: #f58025 !important;border-top-style: solid !important;border-bottom-color: #f58025 !important;border-bottom-style: solid !important;}&#8221;]<\/p>\n<h2>Reasons to choose Monarch for RNA Cleanup:<\/h2>\n<ul>\n<li>Choose from 3 different binding capacities and elution volumes<\/li>\n<li>Quickly and easily purify large quantities of high-quality RNA from in vitro transcription (IVT) reactions<\/li>\n<li>Efficiently remove unincorporated nucleotides from your RNA sample<\/li>\n<\/ul>\n<p>[\/vc_column_text][vc_text_separator title=&#8221;Video: RNA Purification Tips&#8221; color=&#8221;custom&#8221; accent_color=&#8221;#eb7e2d&#8221;][vc_video link=&#8221;https:\/\/www.youtube.com\/watch?v=qgHytx1kIy0&#8243;][\/vc_column][\/vc_row][vc_row bwd_tbc4vd_show_weekday=&#8221;&#8221; disable_element=&#8221;yes&#8221;][vc_column][vc_separator color=&#8221;custom&#8221; accent_color=&#8221;#eb7e2d&#8221;]<div id=\"shorten-68312\" class=\"shorten\" style=\"max-height:200px;\">[vc_column_text]<style>.wForm form{text-align: left;}<\/style><!-- FORM: HEAD SECTION -->\n    <meta http-equiv=\"Content-Type\" content=\"text\/html; charset=utf-8\" \/>\n    <meta name=\"referrer\" content=\"no-referrer-when-downgrade\">\n    <!-- THIS SCRIPT NEEDS TO BE LOADED FIRST BEFORE wforms.js -->\n    <script type=\"text\/javascript\" data-for=\"FA__DOMContentLoadedEventDispatch\" 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class=\"htmlSection\" id=\"tfa_7\"><div class=\"htmlContent\" id=\"tfa_7-HTML\"><h2>Order a&nbsp;<span style=\"color:#eb7e2d;\">Monarch<\/span> DNA- \/ RNA-Purification kit sample!<\/h2><\/div><\/div>\n<div class=\"htmlSection\" id=\"tfa_55\"><div class=\"htmlContent\" id=\"tfa_55-HTML\">Field marked <span style=\"color: red;\">*<\/span> are required<\/div><\/div>\n<div class=\"oneField field-container-D    \" id=\"tfa_43-D\" role=\"group\" aria-labelledby=\"tfa_43-L\" data-tfa-labelledby=\"-L tfa_43-L\">\n<label id=\"tfa_43-L\" class=\"label preField \" data-tfa-check-label-for=\"tfa_43\">Choose your Monarch sample<\/label><br><div class=\"inputWrapper\"><span id=\"tfa_43\" class=\"choices vertical \"><span class=\"oneChoice\"><input type=\"checkbox\" value=\"tfa_89\" class=\"calc-ORDEREDSAMPLES calcval-T1110G\\u0020Monarch\\u0020Spin\\u0020Plasmid\\u0020Miniprep\\u0020Kit\\u0020ACHTUNG:\\u0020Qualifizierung\\u0020durch\\u0020Backoffice\\n\" id=\"tfa_89\" name=\"tfa_89\" aria-labelledby=\"tfa_89-L\" data-tfa-labelledby=\"tfa_43-L tfa_89-L\" data-tfa-parent-id=\"tfa_43\"><label class=\"label postField\" id=\"tfa_89-L\" for=\"tfa_89\"><span class=\"input-checkbox-faux\"><\/span>Monarch Spin Plasmid Miniprep Kit<\/label><\/span><span class=\"oneChoice\"><input type=\"checkbox\" value=\"tfa_46\" class=\"calc-ORDEREDSAMPLES calcval-T1120G\\u0020Monarch\\u0020DNA\\u0020Gel\\u0020Extraction\\u0020Kit\\u0020ACHTUNG:\\u0020Qualifizierung\\u0020durch\\u0020Backoffice\\n\" id=\"tfa_46\" name=\"tfa_46\" aria-labelledby=\"tfa_46-L\" data-tfa-labelledby=\"tfa_43-L tfa_46-L\" data-tfa-parent-id=\"tfa_43\"><label class=\"label postField\" id=\"tfa_46-L\" for=\"tfa_46\"><span class=\"input-checkbox-faux\"><\/span>Monarch Spin DNA Gel Extraction Kit<\/label><\/span><span class=\"oneChoice\"><input type=\"checkbox\" value=\"tfa_48\" class=\"calc-ORDEREDSAMPLES calcval-T1130G\\u0020Monarch\\u0020PCR\\u0020DNA\\u0020Cleanup\\u0020Kit\\u0020ACHTUNG:\\u0020Qualifizierung\\u0020durch\\u0020Backoffice\\n\" id=\"tfa_48\" name=\"tfa_48\" aria-labelledby=\"tfa_48-L\" data-tfa-labelledby=\"tfa_43-L tfa_48-L\" data-tfa-parent-id=\"tfa_43\"><label class=\"label postField\" id=\"tfa_48-L\" for=\"tfa_48\"><span class=\"input-checkbox-faux\"><\/span>Monarch Spin PCR &amp; DNA Cleanup Kit (5\u00b5g)<\/label><\/span><span class=\"oneChoice\"><input type=\"checkbox\" value=\"tfa_90\" class=\"calc-ORDEREDSAMPLES calcval-T1135G\\u0020Monarch\\u0020Spin\\u0020High-Capacity\\u0020 DNA\\u0020Kit\\u0020 Cleanup\\u0020Kit\\u0020(A-Sample!!!)\\n\" id=\"tfa_90\" name=\"tfa_90\" aria-labelledby=\"tfa_90-L\" data-tfa-labelledby=\"tfa_43-L tfa_90-L\" data-tfa-parent-id=\"tfa_43\"><label class=\"label postField\" id=\"tfa_90-L\" for=\"tfa_90\"><span class=\"input-checkbox-faux\"><\/span>Monarch Spin High-Capacity DNA Cleanup Kit (100 \u00b5g)<\/label><\/span><span class=\"oneChoice\"><input type=\"checkbox\" value=\"tfa_84\" class=\"calc-ORDEREDSAMPLES calcval-T3010G\\u0020Monarch\\u0020Genomic\\u0020DNA\\u0020Purification\\u0020Kit\\n\" id=\"tfa_84\" name=\"tfa_84\" aria-labelledby=\"tfa_84-L\" data-tfa-labelledby=\"tfa_43-L tfa_84-L\" data-tfa-parent-id=\"tfa_43\"><label class=\"label postField\" id=\"tfa_84-L\" for=\"tfa_84\"><span class=\"input-checkbox-faux\"><\/span>Monarch Genomic DNA Purification Kit<\/label><\/span><span class=\"oneChoice\"><input type=\"checkbox\" value=\"tfa_87\" class=\"calc-ORDEREDSAMPLES calcval-T4010G\\u0020Monarch\\u0020MagViral\\u0020Kit\\u0020ACHTUNG:\\u0020Qualifizierung\\u0020durch\\u0020Christine\\u0020Bauer\\n\" id=\"tfa_87\" name=\"tfa_87\" aria-labelledby=\"tfa_87-L\" data-tfa-labelledby=\"tfa_43-L tfa_87-L\" data-tfa-parent-id=\"tfa_43\"><label class=\"label postField\" id=\"tfa_87-L\" for=\"tfa_87\"><span class=\"input-checkbox-faux\"><\/span>Monarch Mag Viral DNA\/RNA Extraction Kit<\/label><\/span><\/span><\/div>\n<\/div>\n<div class=\"oneField field-container-D    \" id=\"tfa_64-D\" role=\"group\" aria-labelledby=\"tfa_64-L\" data-tfa-labelledby=\"-L tfa_64-L\">\n<label id=\"tfa_64-L\" class=\"label preField \" data-tfa-check-label-for=\"tfa_64\">For extraction of High Molecular Weight DNA (i.e. for Long Read Sequencing):<\/label><br><div class=\"inputWrapper\"><span id=\"tfa_64\" class=\"choices vertical \"><span class=\"oneChoice\"><input type=\"checkbox\" value=\"tfa_65\" class=\"calc-ORDEREDSAMPLESHMW calcval-T3050G\\u0020Monarch\\u0020HMW\\u0020DNA\\u0020Extraction\\u0020Kit\\u0020for\\u0020Cells\\u0020Blood\\u0020(A-Sample!!!)\\n\" id=\"tfa_65\" name=\"tfa_65\" aria-labelledby=\"tfa_65-L\" data-tfa-labelledby=\"tfa_64-L tfa_65-L\" data-tfa-parent-id=\"tfa_64\"><label class=\"label postField\" id=\"tfa_65-L\" for=\"tfa_65\"><span class=\"input-checkbox-faux\"><\/span>Monarch HMW DNA Extraction Kit for Cells &amp; Blood<\/label><\/span><span class=\"oneChoice\"><input type=\"checkbox\" value=\"tfa_88\" class=\"calc-ORDEREDSAMPLESHMW calcval-T3060G\\u0020Monarch\\u0020HMW\\u0020DNA\\u0020Extraction\\u0020Kit\\u0020for\\u0020Tissue\\u0020(A-Sample!!!)\\n\" id=\"tfa_88\" name=\"tfa_88\" aria-labelledby=\"tfa_88-L\" data-tfa-labelledby=\"tfa_64-L tfa_88-L\" data-tfa-parent-id=\"tfa_64\"><label class=\"label postField\" id=\"tfa_88-L\" for=\"tfa_88\"><span class=\"input-checkbox-faux\"><\/span>Monarch HMW DNA Extraction Kit for Tissue<\/label><\/span><\/span><\/div>\n<\/div>\n<div class=\"htmlSection\" id=\"tfa_51\"><div class=\"htmlContent\" id=\"tfa_51-HTML\"><div style=\"\"><\/div><span style=\"font-size: 11px;\"><i>This offer is limited to new customers and to 1 sample per working group. Samples are available as long as supplies last. Standard DNA samples contain 10 preps, RNA samples and HMW DNA samples contain 5 preps.\n<\/i><\/span><\/div><\/div>\n<div class=\"htmlSection\" id=\"tfa_53\"><div class=\"htmlContent\" id=\"tfa_53-HTML\"><hr><\/div><\/div>\n<div class=\"htmlSection\" id=\"tfa_52\"><div class=\"htmlContent\" id=\"tfa_52-HTML\"><span style=\"font-size:1.2em;font-weight:bold;color:#5c6b80;\">Your institutional address:\n<\/span><\/div><\/div>\n<div id=\"tfa_8\" class=\"section column group\"><table class=\"columnLayout\">\n<tr>\n<td><div class=\"oneField field-container-D    \" id=\"tfa_40-D\">\n<label id=\"tfa_40-L\" class=\"label preField reqMark\" for=\"tfa_40\">E-Mail<\/label><br><div class=\"inputWrapper\"><input aria-required=\"true\" type=\"text\" id=\"tfa_40\" name=\"tfa_40\" value=\"\" autocomplete=\"off\" title=\"E-Mail\" class=\"validate-email required\"><\/div>\n<\/div><\/td>\n<td><div class=\"oneField field-container-D     wf-acl-hidden\" id=\"tfa_42-D\">\n<label id=\"tfa_42-L\" 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accent_color=&#8221;#eb7e2d&#8221;][vc_column_text]<\/p>\n<h2>Typical applications<\/h2>\n<table border=\"0\" width=\"760\">\n<tbody>\n<tr style=\"background-color: #f4f1e7;\">\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><strong>RNA Cleanup and Concentration (including from the TRIzol aqueous phase)<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">RNA purified by other methods can be further purified<\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><strong>Enzymatic Reaction Cleanup<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Enzymes such as RNA polymerases, DNase I, Proteinase K and phosphatases are removed allowing efficient desalting<\/td>\n<\/tr>\n<tr style=\"background-color: #f4f1e7;\">\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><strong><em>In vitro <\/em>Transcription Cleanup<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Enzymes and excess NTPs are removed to yield highly pure synthesized RNA<\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><strong>RNA Gel Extraction<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Purification of RNA from agarose gels<\/td>\n<\/tr>\n<tr style=\"background-color: #f4f1e7;\">\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><strong>RNA Fractionation<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Fractionation of RNA into small and large RNA pools<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_text_separator title=&#8221;Customer feedback&#8221; i_icon_fontawesome=&#8221;fa fa-comment-o&#8221; i_color=&#8221;custom&#8221; color=&#8221;custom&#8221; add_icon=&#8221;true&#8221; i_custom_color=&#8221;#eb7e2d&#8221; accent_color=&#8221;#eb7e2d&#8221;]<div id=\"shorten-9513\" class=\"shorten\" style=\"max-height:250px;\">[vc_row_inner][vc_column_inner width=&#8221;1\/2&#8243;]\n\t<table class=\"balloon\">\n\t<tbody>\n\t<tr>\n\t<td class=\"top_left\"><\/td>\n\t<td class=\"top\" colspan=\"3\"><\/td>\n\t<td class=\"top_right\"><\/td>\n\t<\/tr>\n\t<tr>\n\t<td class=\"left\"><\/td>\n\t<td class=\"content\" colspan=\"3\">\n\t<p style=\"text-align: center;\">\u201eSample Type: Yeast (fresh mycelium) (compared to: Qiagen RNeasy Mini Kit) \u201cBetter yield and quality; easy protocol and good and consistent results among samples.\u201d\u201c<\/p>\n\t<p style=\"text-align: center;\"><strong>Gisele, Research Associate, Mississippi State University<\/strong><\/p>\n\t<\/td>\n\t<td class=\"right\"><\/td>\n\t<\/tr>\n\t<tr>\n\t<td class=\"bottom_left\"><\/td>\n\t<td class=\"bottom\" style=\"width: 60px;\"><\/td>\n\t<td class=\"arrow\"><img decoding=\"async\" src=\"\/wp-content\/themes\/neb-online-theme\/library\/images\/stories\/arrow.png\" alt=\"\" \/><\/td>\n\t<td class=\"bottom\" style=\"width: 507px;\"><\/td>\n\t<td class=\"bottom_right\"><\/td>\n\t<\/tr>\n\t<\/tbody>\n\t<\/table>[\/vc_column_inner][vc_column_inner width=&#8221;1\/2&#8243;]\n\t<table class=\"balloon\">\n\t<tbody>\n\t<tr>\n\t<td class=\"top_left\"><\/td>\n\t<td class=\"top\" colspan=\"3\"><\/td>\n\t<td class=\"top_right\"><\/td>\n\t<\/tr>\n\t<tr>\n\t<td class=\"left\"><\/td>\n\t<td class=\"content\" colspan=\"3\">\n\t<p style=\"text-align: center;\">\u201eSample Type: Gram Positive Bacteria (compared to: Qiagen RNeasy Mini) \u201cI've been using the RNeasy kit for some time, but I've been unable to have much luck. It seems geared toward eukaryotes and I've been working with Gram Positive prokaryotes\u2026 I tried the NEB `{`Total`}` RNA Miniprep Kit and it was much more straightforward. I also got excellent results the first time I used it.\u201d\u201c<\/p>\n\t<p style=\"text-align: center;\"><strong>Kyle, Postdoctoral Researcher, University of CA, Berkeley<\/strong><\/p>\n\t<\/td>\n\t<td class=\"right\"><\/td>\n\t<\/tr>\n\t<tr>\n\t<td class=\"bottom_left\"><\/td>\n\t<td class=\"bottom\" style=\"width: 60px;\"><\/td>\n\t<td class=\"arrow\"><img decoding=\"async\" src=\"\/wp-content\/themes\/neb-online-theme\/library\/images\/stories\/arrow.png\" alt=\"\" \/><\/td>\n\t<td class=\"bottom\" style=\"width: 507px;\"><\/td>\n\t<td class=\"bottom_right\"><\/td>\n\t<\/tr>\n\t<\/tbody>\n\t<\/table>[\/vc_column_inner][\/vc_row_inner][vc_row_inner][vc_column_inner width=&#8221;1\/2&#8243;]\n\t<table class=\"balloon\">\n\t<tbody>\n\t<tr>\n\t<td class=\"top_left\"><\/td>\n\t<td class=\"top\" colspan=\"3\"><\/td>\n\t<td class=\"top_right\"><\/td>\n\t<\/tr>\n\t<tr>\n\t<td class=\"left\"><\/td>\n\t<td class=\"content\" colspan=\"3\">\n\t<p style=\"text-align: center;\">\u201eSample Type: CD34 Cells \u201cI repeated the RNA extraction using `{`NEB\u2019s Monarch Total RNA Miniprep Kit`}`. The quality of RNAs were checked using Tape Station. And all four samples had RIN between 8.3 to 9.3, so they are all good\u2026Your RNA extraction kit is easy to use and saves time.\u201d\u201c<\/p>\n\t<p style=\"text-align: center;\"><strong>Researcher, UCL Great Ormond Street Institute of Child Health<\/strong><\/p>\n\t<\/td>\n\t<td class=\"right\"><\/td>\n\t<\/tr>\n\t<tr>\n\t<td class=\"bottom_left\"><\/td>\n\t<td class=\"bottom\" style=\"width: 60px;\"><\/td>\n\t<td class=\"arrow\"><img decoding=\"async\" src=\"\/wp-content\/themes\/neb-online-theme\/library\/images\/stories\/arrow.png\" alt=\"\" \/><\/td>\n\t<td class=\"bottom\" style=\"width: 507px;\"><\/td>\n\t<td class=\"bottom_right\"><\/td>\n\t<\/tr>\n\t<\/tbody>\n\t<\/table>[\/vc_column_inner][vc_column_inner width=&#8221;1\/2&#8243;]\n\t<table class=\"balloon\">\n\t<tbody>\n\t<tr>\n\t<td class=\"top_left\"><\/td>\n\t<td class=\"top\" colspan=\"3\"><\/td>\n\t<td class=\"top_right\"><\/td>\n\t<\/tr>\n\t<tr>\n\t<td class=\"left\"><\/td>\n\t<td class=\"content\" colspan=\"3\">\n\t<p style=\"text-align: center;\">\u201eSample Type: Oat Protoplast Cells (compared to Qiagen RNeasy Plant Mini Kit) \u201cI compared the yield of RNA extracted and the purity of the RNA based on spectrometer (Nanodrop). When I compared to my usual RNeasy kit from Qiagen to the Monarch sample, I obtain 5 times more RNA from my sample as well as higher purity of my sample with the Monarch RNA mini-prep.\u201d\u201c<\/p>\n\t<p style=\"text-align: center;\"><strong>Researcher, Iowa State University<\/strong><\/p>\n\t<\/td>\n\t<td class=\"right\"><\/td>\n\t<\/tr>\n\t<tr>\n\t<td class=\"bottom_left\"><\/td>\n\t<td class=\"bottom\" style=\"width: 60px;\"><\/td>\n\t<td class=\"arrow\"><img decoding=\"async\" src=\"\/wp-content\/themes\/neb-online-theme\/library\/images\/stories\/arrow.png\" alt=\"\" \/><\/td>\n\t<td class=\"bottom\" style=\"width: 507px;\"><\/td>\n\t<td class=\"bottom_right\"><\/td>\n\t<\/tr>\n\t<\/tbody>\n\t<\/table>[\/vc_column_inner][\/vc_row_inner][vc_row_inner][vc_column_inner width=&#8221;1\/2&#8243;]\n\t<table class=\"balloon\">\n\t<tbody>\n\t<tr>\n\t<td class=\"top_left\"><\/td>\n\t<td class=\"top\" colspan=\"3\"><\/td>\n\t<td class=\"top_right\"><\/td>\n\t<\/tr>\n\t<tr>\n\t<td class=\"left\"><\/td>\n\t<td class=\"content\" colspan=\"3\">\n\t<p style=\"text-align: center;\">\u201eSample Type: Mouse Heart (compared to: Thermo Fisher Scientific GeneJet RNA Isolation Kit) ``I liked the consistency, ease of use, and the fact that everything you need (e.g. DNase, Proteinase K) comes in the kit. Both the concentration and quality of the isolated RNA from the Monarch Kit were slightly better than the GeneJet kit. The bigger difference was in the consistency of the RNA isolations between samples--it was much better with the Monarch Kit.``\u201c<\/p>\n\t<p style=\"text-align: center;\"><strong>Natalie, PhD. Student, Winnipeg, CA<\/strong><\/p>\n\t<\/td>\n\t<td class=\"right\"><\/td>\n\t<\/tr>\n\t<tr>\n\t<td class=\"bottom_left\"><\/td>\n\t<td class=\"bottom\" style=\"width: 60px;\"><\/td>\n\t<td class=\"arrow\"><img decoding=\"async\" src=\"\/wp-content\/themes\/neb-online-theme\/library\/images\/stories\/arrow.png\" alt=\"\" \/><\/td>\n\t<td class=\"bottom\" style=\"width: 507px;\"><\/td>\n\t<td class=\"bottom_right\"><\/td>\n\t<\/tr>\n\t<\/tbody>\n\t<\/table>[\/vc_column_inner][vc_column_inner width=&#8221;1\/2&#8243;]\n\t<table class=\"balloon\">\n\t<tbody>\n\t<tr>\n\t<td class=\"top_left\"><\/td>\n\t<td class=\"top\" colspan=\"3\"><\/td>\n\t<td class=\"top_right\"><\/td>\n\t<\/tr>\n\t<tr>\n\t<td class=\"left\"><\/td>\n\t<td class=\"content\" colspan=\"3\">\n\t<p style=\"text-align: center;\">\u201eSample Type: RNA from Virus in cell culture supernatant (compared to: TRIzol extraction) \u201cI\u2019ve had a go extracting RNA from a couple of different samples using the Monarch kit, in parallel with Trizol extractions from duplicate samples. The initial results, nanodrop analysis of the extracted RNA, were very positive.\u201d\u201c<\/p>\n\t<p style=\"text-align: center;\"><strong>Jessica Purcell, Pirbright Institute, United Kingdom<\/strong><\/p>\n\t<\/td>\n\t<td class=\"right\"><\/td>\n\t<\/tr>\n\t<tr>\n\t<td class=\"bottom_left\"><\/td>\n\t<td class=\"bottom\" style=\"width: 60px;\"><\/td>\n\t<td class=\"arrow\"><img decoding=\"async\" src=\"\/wp-content\/themes\/neb-online-theme\/library\/images\/stories\/arrow.png\" alt=\"\" \/><\/td>\n\t<td class=\"bottom\" style=\"width: 507px;\"><\/td>\n\t<td class=\"bottom_right\"><\/td>\n\t<\/tr>\n\t<\/tbody>\n\t<\/table>[\/vc_column_inner][\/vc_row_inner]<\/div><a href=\"#\" id=\"btn-shorten-9513\" class=\"shorten-btn\">Show more<\/a><style>\n\t\t#shorten-9513::after{\n\t\t\tbackground: linear-gradient(to bottom, rgba(255,255,255,0), #ffffff);\n\t\t\tbackground: -moz-linear-gradient(top, rgba(255,255,255,0), #ffffff);\n\t\t\tbackground: -webkit-linear-gradient(top, rgba(255,255,255,0), #ffffff);\n\t\t}\n\t<\/style><script>\n\t\t\t\t\t\t\t\t\tjQuery(document).ready(function($) {\n\t\t\t\t\t\t\t\t\t\t$(\"#btn-shorten-9513\").click(function(event) {\n\t\t\t\t\t\t\t\t\t\t\tevent.preventDefault();\n\t\t\t\t\t\t\t\t\t\t\tif($(\"#shorten-9513\").hasClass(\"open\")){\n\t\t\t\t\t\t\t\t\t\t\t\t$(\"#shorten-9513\").removeClass(\"open\");\n\t\t\t\t\t\t\t\t\t\t\t\t$(\"#btn-shorten-9513\").html(\"Show more\");\n\t\t\t\t\t\t\t\t\t\t\t} else {\n\t\t\t\t\t\t\t\t\t\t\t\t$(\"#shorten-9513\").addClass(\"open\");\n\t\t\t\t\t\t\t\t\t\t\t\t$(\"#btn-shorten-9513\").html(\"Hide\");\n\t\t\t\t\t\t\t\t\t\t\t}\n\t\t\t\t\t\t\t\t\t\t});\n\t\t\t\t\t\t\t\t\t});\n\t\t\t\t\t\t\t<\/script>[\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=&#8221;custom&#8221; accent_color=&#8221;#eb7e2d&#8221;]<div id=\"shorten-19927\" class=\"shorten\" style=\"max-height:600px;\">[vc_column_text]<\/p>\n<h2>Flexibility in Binding Capacity and Elution Volume<\/h2>\n<p>[\/vc_column_text][vc_column_text]<\/p>\n<table style=\"border-collapse: collapse;\" cellspacing=\"0px\">\n<thead>\n<tr style=\"font-size: 1.1em; background-color: #b3b3b3;\">\n<th style=\"padding-top: 10px; padding-bottom: 10px; padding-left: 3px; border: 1px solid #999;\" align=\"left\" width=\"25%\"><strong>Monarch Spin RNA Cleanup Kit<\/strong><\/th>\n<th style=\"padding-top: 10px; padding-bottom: 10px; border: 1px solid #999;\" width=\"25%\"><strong>NEB #T2030 (10 \u00b5g)<\/strong><\/th>\n<th style=\"padding-top: 10px; padding-bottom: 10px; border: 1px solid #999;\" width=\"25%\"><strong>NEB #T2040 (50 \u00b5g)<\/strong><\/th>\n<th style=\"padding-top: 10px; padding-bottom: 10px; border: 1px solid #999;\" width=\"25%\"><strong>NEB #T2050 (500 \u00b5g)<\/strong><\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr style=\"background-color: #e5e5e5;\">\n<td style=\"border: 1px solid #999;\"><strong>Binding Capacity:<\/strong><\/td>\n<td style=\"border: 1px solid #999;\">10 \u03bcg<\/td>\n<td style=\"border: 1px solid #999;\">50 \u00b5g<\/td>\n<td style=\"border: 1px solid #999;\">500 \u00b5g<\/td>\n<\/tr>\n<tr>\n<td style=\"border: 1px solid #999;\"><strong>RNA Size Range:<\/strong><\/td>\n<td style=\"text-align: center; border: 1px solid #999;\" colspan=\"3\">\u2265 25 nt ( \u2265 15 nt with modified protocol)<\/td>\n<\/tr>\n<tr style=\"background-color: #e5e5e5;\">\n<td style=\"text-align: left; border: 1px solid #999;\"><strong>Typical Recovery:<\/strong><\/td>\n<td style=\"text-align: center; border: 1px solid #999;\" colspan=\"3\">70\u2013100%<\/td>\n<\/tr>\n<tr>\n<td style=\"border: 1px solid #999;\"><strong>Elution Volume:<\/strong><\/td>\n<td style=\"border: 1px solid #999;\">6\u201320 \u00b5l<\/td>\n<td style=\"border: 1px solid #999;\">20\u201350 \u00b5l<\/td>\n<td style=\"border: 1px solid #999;\">50\u2013100 \u00b5l<\/td>\n<\/tr>\n<tr style=\"background-color: #e5e5e5;\">\n<td style=\"border: 1px solid #999;\"><strong>Purity:<\/strong><\/td>\n<td style=\"text-align: center; border: 1px solid #999;\" colspan=\"3\">A<sub>260\/280<\/sub> &gt; 1.8 and A<sub>260\/230<\/sub> &gt; 1.8<\/td>\n<\/tr>\n<tr>\n<td style=\"border: 1px solid #999;\"><strong>Protocol Time:<\/strong><\/td>\n<td style=\"text-align: center; border: 1px solid #999;\" colspan=\"2\">5 minutes of spin and incubation time<\/td>\n<td style=\"border: 1px solid #999;\">10\u201315 minutes of spin and incubation time<\/td>\n<\/tr>\n<tr style=\"background-color: #e5e5e5;\">\n<td style=\"border: 1px solid #999;\"><strong>Common Downstream Applications:<\/strong><\/td>\n<td style=\"border: 1px solid #999;\">RT-PCR, RNA library prep for NGS, Small RNA library prep for NGS, RNA labeling<\/td>\n<td style=\"border: 1px solid #999;\">RT-PCR, RNA library prep for NGS, formation of RNP complexes for genome editing, microinjection, RNA labeling, transfection<\/td>\n<td style=\"border: 1px solid #999;\">RT-PCR, RNA library prep for NGS, RNA labeling, RNAi, microinjection, transfection<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>[\/vc_column_text][vc_separator][vc_row_inner][vc_column_inner][vc_column_text]<\/p>\n<h2>Purify Large Amounts of RNA from IVT Reactions<\/h2>\n<p>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner][vc_column_inner width=&#8221;1\/2&#8243;][vc_column_text]<span class=\"bildunterschrift\">rRNA (10, 50 or 500 \u00b5g, respectively of 16S and 23S Ribosomal Standard from E. coli, Sigma) was purified using a Monarch RNA Cleanup Kit (10 \u00b5g, NEB #T2030) (50 \u00b5g, NEB #T2040) (500 \u00b5g, NEB #T2050). Nuclease-free water was used to elute the RNA. The percent recovery of the RNA was calculated from the resulting A260 as measured using a Trinean DropSense 16. ~80% of RNA can be efficiently recovered in 6 \u00b5l from the Monarch RNA Cleanup Kit (10 \u00b5g, NEB #T2030), 20 \u00b5l from the Monarch RNA Cleanup Kit (50 \u00b5g, NEB #T2040), and 50 \u00b5l from the Monarch RNA Cleanup Kit (500 \u00b5g, NEB #T2050).<\/span>[\/vc_column_text][\/vc_column_inner][vc_column_inner width=&#8221;1\/2&#8243;][vc_single_image image=&#8221;15731&#8243; img_size=&#8221;full&#8221; onclick=&#8221;link_image&#8221;][\/vc_column_inner][\/vc_row_inner][vc_separator][vc_row_inner][vc_column_inner][vc_column_text]<strong>The Monarch RNA Cleanup Kit (500 \u00b5g) is suitable for cleaning up large quantities (&gt;250 \u00b5g) of RNA from <em>in vitro<\/em> transcription reactions<\/strong>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner][vc_column_inner width=&#8221;1\/2&#8243;][vc_column_text]<span class=\"bildunterschrift\">1. RNA transcripts of varying sizes (0.6-8 kb) were synthesized using the HiScribe\u2122 T7 Quick High Yield RNA Synthesis Kit (NEB #E2050) using 1.5-1.8 \u00b5g of DNA template for two hours at 37\u00b0C. 40 \u00b5l of each in vitro transcription IVT) reaction was cleaned up using the Monarch RNA Cleanup Kit (500 \u00b5g, T2050) and eluted in 200 \u00b5l. RNA yields were calculated from the resulting A260, measured using a Nanodrop\u00ae spectrophotometer and ranged from 268-425 \u00b5g of RNA per IVT reaction.<\/span><\/p>\n<p><span class=\"bildunterschrift\">2. RNA integrity (200 ng\/lane) was assessed on a 1% agarose-TBE gel stained with SYBR\u00ae Gold.<\/span>[\/vc_column_text][\/vc_column_inner][vc_column_inner width=&#8221;1\/2&#8243;][vc_single_image image=&#8221;15738&#8243; img_size=&#8221;full&#8221; onclick=&#8221;link_image&#8221;][\/vc_column_inner][\/vc_row_inner][vc_separator][vc_row_inner][vc_column_inner][vc_column_text]<strong>The Monarch RNA Cleanup Kit (50 \u00b5g) produces sgRNA yields consistent with other competitor RNA cleanup kits and with lower residual NTP contamination<\/strong>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner][vc_column_inner width=&#8221;1\/2&#8243;][vc_column_text]<span class=\"bildunterschrift\">Six different sgRNA synthesis reactions from the <a href=\"http:\/\/www.neb-online.de\/shop\/neb_de_product.php?productnumber=E3322\" target=\"_blank\" rel=\"noopener noreferrer\">EnGen\u00ae sgRNA Synthesis Kit, <em>S. pyogenes<\/em> (NEB #E3322)<\/a> were cleaned up using either the Monarch RNA Cleanup Kit (50 \u00b5g, NEB #T2040) or a competitor kit (according to manufacturer\u2019s recommendations) and eluted with 50 \u00b5l of nuclease-free water. sgRNA yield was calculated from the resulting A260, as measured using a Trinean Dropsense\u2122 16. The Monarch Cleanup Kit produced sgRNA yields consistent with other commercially available RNA cleanup kits.<\/span><\/p>\n<p><span class=\"bildunterschrift\">Following cleanup, residual nucleotides (NTPs) were measured by LC-MS and are reported as (percent area NTPs (rATP+rCTP+rGTP+rUTP)\/percent area sgRNA). The Monarch RNA Cleanup Kit consistently outperforms other commercially available RNA cleanup kits in the removal of residual NTPs from sgRNA synthesis reactions.<\/span>[\/vc_column_text][\/vc_column_inner][vc_column_inner width=&#8221;1\/2&#8243;][vc_single_image image=&#8221;15739&#8243; img_size=&#8221;full&#8221; onclick=&#8221;link_image&#8221;][\/vc_column_inner][\/vc_row_inner]<\/div><a href=\"#\" id=\"btn-shorten-19927\" class=\"shorten-btn\">Show more<\/a><style>\n\t\t#shorten-19927::after{\n\t\t\tbackground: linear-gradient(to bottom, rgba(255,255,255,0), #ffffff);\n\t\t\tbackground: -moz-linear-gradient(top, rgba(255,255,255,0), #ffffff);\n\t\t\tbackground: -webkit-linear-gradient(top, rgba(255,255,255,0), #ffffff);\n\t\t}\n\t<\/style><script>\n\t\t\t\t\t\t\t\t\tjQuery(document).ready(function($) {\n\t\t\t\t\t\t\t\t\t\t$(\"#btn-shorten-19927\").click(function(event) {\n\t\t\t\t\t\t\t\t\t\t\tevent.preventDefault();\n\t\t\t\t\t\t\t\t\t\t\tif($(\"#shorten-19927\").hasClass(\"open\")){\n\t\t\t\t\t\t\t\t\t\t\t\t$(\"#shorten-19927\").removeClass(\"open\");\n\t\t\t\t\t\t\t\t\t\t\t\t$(\"#btn-shorten-19927\").html(\"Show more\");\n\t\t\t\t\t\t\t\t\t\t\t} else {\n\t\t\t\t\t\t\t\t\t\t\t\t$(\"#shorten-19927\").addClass(\"open\");\n\t\t\t\t\t\t\t\t\t\t\t\t$(\"#btn-shorten-19927\").html(\"Hide\");\n\t\t\t\t\t\t\t\t\t\t\t}\n\t\t\t\t\t\t\t\t\t\t});\n\t\t\t\t\t\t\t\t\t});\n\t\t\t\t\t\t\t<\/script>[\/vc_column][\/vc_row][vc_row bwd_show_when_type=&#8221;after&#8221; bwd_show_when_date=&#8221;01\/01\/2023 01:00&#8243; bwd_tbc4vd_show_weekday=&#8221;&#8221;][vc_column][vc_separator color=&#8221;custom&#8221; accent_color=&#8221;#eb7e2d&#8221;][vc_column_text]<\/p>\n<h2>Available RNA Purification Kits:<\/h2>\n<table style=\"width: 760px;\" border=\"0\" cellpadding=\"5\">\n<tbody>\n<tr style=\"background-color: #f3f3f3; font-size: 16px;\">\n<th style=\"border-bottom: solid #eb7e2d;\" align=\"left\" width=\"400\" height=\"40\">PRODUCT<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"88\">Prod. #<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"100\">SIZE<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" colspan=\"2\">INFO<\/th>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\">Monarch Spin RNA Cleanup Kit (10 \u00b5g)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">T2030S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">10 preps<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/T2030\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/T2030\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">T2030L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 preps<\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\">Monarch Spin RNA Cleanup Kit (50 \u00b5g)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">T2040S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">10 preps<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/T2040\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/t2040\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">T2040L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 preps<\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\">Monarch Spin RNA Cleanup Kit (500 \u00b5g)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">T2050S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">10 preps<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/T2050\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/t2050\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">T2050L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 preps<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p style=\"text-align: right;\"><span class=\"bildunterschrift\">As of: 01.01.2024<\/span><\/p>\n<p>[\/vc_column_text][vc_column_text]<\/p>\n<h2>Also available:<\/h2>\n<table style=\"width: 760px;\" border=\"0\" cellpadding=\"5\">\n<tbody>\n<tr style=\"background-color: #f3f3f3;\">\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"400\" height=\"40\">PRODUCT<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"88\">Prod.Nr.:<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"100\">SIZE<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" colspan=\"2\">INFO<\/th>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Monarch Spin Columns S1A and Tubes (10 \u00b5g)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">T2037L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 preps<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/T2037\" target=\"_blank\" rel=\"noopener\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb.com\/en\/t2037\" target=\"_blank\" rel=\"noopener\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"NEB Info_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Monarch Buffer BX<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">T2041L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">80 ml<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/T2041\" target=\"_blank\" rel=\"noopener\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb.com\/en\/t2041\" target=\"_blank\" rel=\"noopener\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"NEB Info_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Monarch Buffer WX<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">T2042L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">40 ml<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/T2042\" target=\"_blank\" rel=\"noopener\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb.com\/en\/t2042\" target=\"_blank\" rel=\"noopener\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"NEB Info_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Monarch Spin Columns S2A and Tubes (50 \u00b5g)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">T2047L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 preps<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/T2047\" target=\"_blank\" rel=\"noopener\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb.com\/en\/t2047\" target=\"_blank\" rel=\"noopener\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"NEB Info_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Monarch Spin Columns S2B and Tubes (500 \u00b5g)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">T2057L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 preps<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/T2057\" target=\"_blank\" rel=\"noopener\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb.com\/en\/t2057\" target=\"_blank\" rel=\"noopener\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"NEB Info_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p style=\"text-align: right;\"><span class=\"bildunterschrift\">As of: 07.05.2024<\/span><\/p>\n<p>[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row css=&#8221;.vc_custom_1533728291740{margin-bottom: 30px !important;}&#8221;][vc_column][vc_basic_grid post_type=&#8221;post&#8221; max_items=&#8221;3&#8243; element_width=&#8221;12&#8243; item=&#8221;4123&#8243; grid_id=&#8221;vc_gid:1737042147429-dcd053e7-1423-6&#8243; taxonomies=&#8221;138, 164&#8243;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text] Monarch Kits for RNA Purification and Concentration The Monarch Spin RNA Cleanup Kits provide a fast and simple silica spin column-based solution for RNA purification and concentration after any enzymatic reaction (including in vitro transcription, DNase I treatment, capping and labeling) and after&#8230;  <a class=\"excerpt-read-more\" href=\"https:\/\/www.neb-online.de\/en\/monarch-nucleic-acid-purification-kits-2\/monarch-rna-cleanup-kits\/\" title=\"Read Monarch Spin RNA Cleanup Kits\">Read more &raquo;<\/a><\/p>\n","protected":false},"author":18,"featured_media":48790,"parent":19288,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-sidebar.php","meta":{"footnotes":""},"class_list":["post-15760","page","type-page","status-publish","has-post-thumbnail","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.1.1 - 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