{"id":1759,"date":"2015-04-29T08:33:30","date_gmt":"2015-04-29T08:33:30","guid":{"rendered":"http:\/\/www.neb-online.de\/?page_id=1759"},"modified":"2024-01-04T14:20:33","modified_gmt":"2024-01-04T13:20:33","slug":"restriction-enzymes-for-epigenetics","status":"publish","type":"page","link":"https:\/\/www.neb-online.de\/en\/epigenetics\/restriction-enzymes-for-epigenetics\/","title":{"rendered":"Restriction Enzymes for Epigenetics"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column][vc_basic_grid post_type=&#8221;post&#8221; max_items=&#8221;10&#8243; element_width=&#8221;6&#8243; item=&#8221;4123&#8243; taxonomies=&#8221;121&#8243; grid_id=&#8221;vc_gid:1704374370298-13179951-7650-0&#8243;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]Many restriction enzymes are sensitive to the DNA methylation states. Cleavage can be blocked or impaired when a particular base in the recognition site is modified.<\/p>\n<p>Scientists at NEB recently identified the <a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=R0661\" target=\"_blank\" rel=\"noopener noreferrer\">MspJI<\/a> family of restriction enzymes, which are dependent on methylation and hydroxymethylation for cleavage to occur. These enzymes excise ~ 32 base pair fragments containing a centrally located 5-hmC or 5-mC modified residue that can be extracted and sequenced. Due to the known position of this epigenetic modification, <a title=\"EpiMark Bisulfite Conversion Kit &amp; Hot Start DNA Polymerase\" href=\"https:\/\/www.neb-online.de\/en\/epigenetics\/epimark-kits\/epimark-bisulfite-conversion-kit-hot-start-dna-polymerase\/\">bisulfite conversion<\/a> is not required prior to downstream analysis.[\/vc_column_text][vc_row_inner][vc_column_inner width=&#8221;1\/2&#8243;][vc_column_text]These EpiMark validated, methylation-dependent restriction enzymes expand the potential for mapping epigenetic modifications and simplify the study of DNA methylation. Additionally, they provide an opportunity to better understand the role of <a title=\"EpiMark 5-mC and 5-hmC Analysis Kit\" href=\"https:\/\/www.neb-online.de\/en\/epigenetics\/epimark-kits#EpiMark5mC_EN\">5-hydroxymethylcytosine<\/a> in the genome.<\/p>\n<p>A variety of our existing restriction enzymes can also be used to study epigenetic modifications of DNA, such as, <a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=R0176\" target=\"_blank\" rel=\"noopener noreferrer\">DpnI<\/a> and <a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=R0543\" target=\"_blank\" rel=\"noopener noreferrer\">DpnII<\/a> that recognize the same sequence, but different methylation patterns. <a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=M0272\" target=\"_blank\" rel=\"noopener noreferrer\">McrBC<\/a> also only cleaves DNA that is methylated on one or both strands.[\/vc_column_text][\/vc_column_inner][vc_column_inner width=&#8221;1\/2&#8243;]<div class=\"vbox\">\n\t\t\t\t<span class=\"vhead\">Advantages:<\/span>\n\t\t\t\t\t<ul class=\"vlist\">\n\t\t\t\t\t\t<li><span>Specificity to epigenetically-relevant DNA modifications (5-mC and 5-hmC)<br \/><\/span><\/li><li><span>Easy-to-use protocols (enzymatic digestion followed by gel extraction)&lt;<br \/><\/span><\/li><li><span>Less harsh than bisulfite conversion<br \/><\/span><\/li><li><span>Simplified data analysis<\/span><\/li>\n\t\t\t\t\t<\/ul>\n\t\t\t<\/div>[\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_single_image image=&#8221;1754&#8243; img_size=&#8221;full&#8221;][\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<span class=\"bildunterschrift\"><em><strong>Simplify DNA methylation analysis with MspJI<br \/>\n<\/strong>MspJI recognizes methylated and hydroxymethylated DNA and cleaves out ~ 32 bp fragments for downstream sequencing analysis. Overnight digestion of 1 \u00b5g of genomic DNA from various sources with or without MspJI is shown. Note: Yeast DNA does not contain methylated DNA and thus, no 32-mer is detected.<\/em><\/span>[\/vc_column_text][\/vc_column][\/vc_row][vc_row bwd_show_when_type=&#8221;after&#8221; bwd_show_when_date=&#8221;01\/01\/2023 01:00&#8243; bwd_tbc4vd_show_weekday=&#8221;&#8221;][vc_column][vc_column_text]<\/p>\n<h2>Product table<\/h2>\n<table style=\"width: 760px;\" border=\"0\" cellpadding=\"5\">\n<tbody>\n<tr style=\"background-color: #f3f3f3; font-size: 16px;\">\n<th style=\"border-bottom: solid #eb7e2d;\" align=\"left\" width=\"400\" height=\"40px\">PRODUCT<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"88\">Prod. #<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"100\">SIZE<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" colspan=\"2\">INFO<\/th>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\">MspJI, recombinant<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">R0661S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">200 units<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/R0661\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/r0661\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">R0661L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">1.000 units<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/R0661\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/r0661\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\">DpnI, recombinant<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">R0176S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">1.000 units<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/R0176\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/r0176\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">R0176L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">5.000 units<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/R0176\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/r0176\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\">DpnII, recombinant<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">R0543S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">1.000 units<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/R0543\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/r0543\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">R0543L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">5.000 units<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/R0543\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/r0543\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\">DpnII, recombinant, conc.<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">R0543T<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">1.000 units<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/R0543\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/r0543\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">R0543M<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">5.000 units<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/R0543\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/r0543\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\">MspI, recombinant<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">R0106S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">5.000 units<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/R0106\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/r0106\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">R0106L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">25.000 units<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/R0106\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/r0106\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\">MspI, recombinant, conc.<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">R0106T<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">5.000 units<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/R0106\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/r0106\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">R0106M<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">25.000 units<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/R0106\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/r0106\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\">HpaII, recombinant<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">R0171S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">2.000 units<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/R0171\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/r0171\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">R0171L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">10.000 units<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/R0171\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/r0171\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">HpaII, recombinant, conc.<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">R0171M<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">10.000 units<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/R0171\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/r0171\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p style=\"text-align: right;\"><em><span class=\"bildunterschrift\">As of: 01.01.2024<\/span><\/em><\/p>\n<p>[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row][vc_column][vc_basic_grid post_type=&#8221;post&#8221; max_items=&#8221;10&#8243; element_width=&#8221;6&#8243; item=&#8221;4123&#8243; taxonomies=&#8221;121&#8243; grid_id=&#8221;vc_gid:1704374370298-13179951-7650-0&#8243;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]Many restriction enzymes are sensitive to the DNA methylation states. Cleavage can be blocked or impaired when a particular base in the recognition site is modified. Scientists at NEB recently identified the MspJI family of restriction enzymes, which are dependent on methylation and hydroxymethylation for cleavage to occur. These&#8230;  <a class=\"excerpt-read-more\" href=\"https:\/\/www.neb-online.de\/en\/epigenetics\/restriction-enzymes-for-epigenetics\/\" title=\"Read Restriction Enzymes for Epigenetics\">Read more &raquo;<\/a><\/p>\n","protected":false},"author":2,"featured_media":40775,"parent":2264,"menu_order":0,"comment_status":"open","ping_status":"closed","template":"page-sidebar.php","meta":{"footnotes":""},"class_list":["post-1759","page","type-page","status-publish","has-post-thumbnail","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.1.1 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Restriction Enzymes for Epigenetics - New England Biolabs GmbH<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.neb-online.de\/en\/epigenetics\/restriction-enzymes-for-epigenetics\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Restriction Enzymes for Epigenetics - New England Biolabs GmbH\" \/>\n<meta property=\"og:description\" content=\"[vc_row][vc_column][vc_basic_grid post_type=&#8221;post&#8221; max_items=&#8221;10&#8243; element_width=&#8221;6&#8243; item=&#8221;4123&#8243; taxonomies=&#8221;121&#8243; grid_id=&#8221;vc_gid:1704374370298-13179951-7650-0&#8243;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]Many restriction enzymes are sensitive to the DNA methylation states. Cleavage can be blocked or impaired when a particular base in the recognition site is modified. Scientists at NEB recently identified the MspJI family of restriction enzymes, which are dependent on methylation and hydroxymethylation for cleavage to occur. These... Read more &raquo;\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.neb-online.de\/en\/epigenetics\/restriction-enzymes-for-epigenetics\/\" \/>\n<meta property=\"og:site_name\" content=\"New England Biolabs GmbH\" \/>\n<meta property=\"article:modified_time\" content=\"2024-01-04T13:20:33+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/04\/NEB_epigenetik_banner_760px.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"760\" \/>\n\t<meta property=\"og:image:height\" content=\"200\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"9 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.neb-online.de\/en\/epigenetics\/restriction-enzymes-for-epigenetics\/\",\"url\":\"https:\/\/www.neb-online.de\/en\/epigenetics\/restriction-enzymes-for-epigenetics\/\",\"name\":\"Restriction Enzymes for Epigenetics - New England Biolabs GmbH\",\"isPartOf\":{\"@id\":\"https:\/\/www.neb-online.de\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/www.neb-online.de\/en\/epigenetics\/restriction-enzymes-for-epigenetics\/#primaryimage\"},\"image\":{\"@id\":\"https:\/\/www.neb-online.de\/en\/epigenetics\/restriction-enzymes-for-epigenetics\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/04\/NEB_epigenetik_banner_760px.jpg\",\"datePublished\":\"2015-04-29T08:33:30+00:00\",\"dateModified\":\"2024-01-04T13:20:33+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/www.neb-online.de\/en\/epigenetics\/restriction-enzymes-for-epigenetics\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/www.neb-online.de\/en\/epigenetics\/restriction-enzymes-for-epigenetics\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/www.neb-online.de\/en\/epigenetics\/restriction-enzymes-for-epigenetics\/#primaryimage\",\"url\":\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/04\/NEB_epigenetik_banner_760px.jpg\",\"contentUrl\":\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/04\/NEB_epigenetik_banner_760px.jpg\",\"width\":760,\"height\":200},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/www.neb-online.de\/en\/epigenetics\/restriction-enzymes-for-epigenetics\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/www.neb-online.de\/en\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Epigenetics\",\"item\":\"https:\/\/www.neb-online.de\/en\/epigenetics\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"Restriction Enzymes for Epigenetics\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/www.neb-online.de\/#website\",\"url\":\"https:\/\/www.neb-online.de\/\",\"name\":\"New England Biolabs GmbH\",\"description\":\"Enabling Technologies in the Life Sciences\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/www.neb-online.de\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Restriction Enzymes for Epigenetics - New England Biolabs GmbH","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.neb-online.de\/en\/epigenetics\/restriction-enzymes-for-epigenetics\/","og_locale":"en_US","og_type":"article","og_title":"Restriction Enzymes for Epigenetics - New England Biolabs GmbH","og_description":"[vc_row][vc_column][vc_basic_grid post_type=&#8221;post&#8221; max_items=&#8221;10&#8243; element_width=&#8221;6&#8243; item=&#8221;4123&#8243; taxonomies=&#8221;121&#8243; grid_id=&#8221;vc_gid:1704374370298-13179951-7650-0&#8243;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]Many restriction enzymes are sensitive to the DNA methylation states. Cleavage can be blocked or impaired when a particular base in the recognition site is modified. Scientists at NEB recently identified the MspJI family of restriction enzymes, which are dependent on methylation and hydroxymethylation for cleavage to occur. These... Read more &raquo;","og_url":"https:\/\/www.neb-online.de\/en\/epigenetics\/restriction-enzymes-for-epigenetics\/","og_site_name":"New England Biolabs GmbH","article_modified_time":"2024-01-04T13:20:33+00:00","og_image":[{"width":760,"height":200,"url":"https:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/04\/NEB_epigenetik_banner_760px.jpg","type":"image\/jpeg"}],"twitter_misc":{"Est. reading time":"9 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/www.neb-online.de\/en\/epigenetics\/restriction-enzymes-for-epigenetics\/","url":"https:\/\/www.neb-online.de\/en\/epigenetics\/restriction-enzymes-for-epigenetics\/","name":"Restriction Enzymes for Epigenetics - New England Biolabs GmbH","isPartOf":{"@id":"https:\/\/www.neb-online.de\/#website"},"primaryImageOfPage":{"@id":"https:\/\/www.neb-online.de\/en\/epigenetics\/restriction-enzymes-for-epigenetics\/#primaryimage"},"image":{"@id":"https:\/\/www.neb-online.de\/en\/epigenetics\/restriction-enzymes-for-epigenetics\/#primaryimage"},"thumbnailUrl":"https:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/04\/NEB_epigenetik_banner_760px.jpg","datePublished":"2015-04-29T08:33:30+00:00","dateModified":"2024-01-04T13:20:33+00:00","breadcrumb":{"@id":"https:\/\/www.neb-online.de\/en\/epigenetics\/restriction-enzymes-for-epigenetics\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.neb-online.de\/en\/epigenetics\/restriction-enzymes-for-epigenetics\/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.neb-online.de\/en\/epigenetics\/restriction-enzymes-for-epigenetics\/#primaryimage","url":"https:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/04\/NEB_epigenetik_banner_760px.jpg","contentUrl":"https:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/04\/NEB_epigenetik_banner_760px.jpg","width":760,"height":200},{"@type":"BreadcrumbList","@id":"https:\/\/www.neb-online.de\/en\/epigenetics\/restriction-enzymes-for-epigenetics\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/www.neb-online.de\/en\/"},{"@type":"ListItem","position":2,"name":"Epigenetics","item":"https:\/\/www.neb-online.de\/en\/epigenetics\/"},{"@type":"ListItem","position":3,"name":"Restriction Enzymes for Epigenetics"}]},{"@type":"WebSite","@id":"https:\/\/www.neb-online.de\/#website","url":"https:\/\/www.neb-online.de\/","name":"New England Biolabs GmbH","description":"Enabling Technologies in the Life Sciences","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/www.neb-online.de\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"}]}},"_links":{"self":[{"href":"https:\/\/www.neb-online.de\/en\/wp-json\/wp\/v2\/pages\/1759","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.neb-online.de\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.neb-online.de\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.neb-online.de\/en\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.neb-online.de\/en\/wp-json\/wp\/v2\/comments?post=1759"}],"version-history":[{"count":20,"href":"https:\/\/www.neb-online.de\/en\/wp-json\/wp\/v2\/pages\/1759\/revisions"}],"predecessor-version":[{"id":46223,"href":"https:\/\/www.neb-online.de\/en\/wp-json\/wp\/v2\/pages\/1759\/revisions\/46223"}],"up":[{"embeddable":true,"href":"https:\/\/www.neb-online.de\/en\/wp-json\/wp\/v2\/pages\/2264"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.neb-online.de\/en\/wp-json\/wp\/v2\/media\/40775"}],"wp:attachment":[{"href":"https:\/\/www.neb-online.de\/en\/wp-json\/wp\/v2\/media?parent=1759"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}