{"id":1863,"date":"2015-04-07T19:23:12","date_gmt":"2015-04-07T19:23:12","guid":{"rendered":"http:\/\/www.neb-online.de\/neb\/cloning-synthetic-biology\/restriktionsenzyme\/time-saver-restriktionsenzyme\/"},"modified":"2015-08-17T10:07:44","modified_gmt":"2015-08-17T08:07:44","slug":"time-saver-restriktionsenzyme","status":"publish","type":"page","link":"https:\/\/www.neb-online.de\/en\/cloning-synthetic-biology\/restriktionsenzyme\/time-saver-restriktionsenzyme\/","title":{"rendered":"Time-Saver Restriction Enzymes"},"content":{"rendered":"

[vc_row][vc_column width=”1\/1″][vc_basic_grid post_type=”post” max_items=”10″ style=”all” items_per_page=”10″ show_filter=”” element_width=”12″ gap=”15″ orderby=”date” order=”DESC” filter_source=”category” filter_style=”default” filter_align=”center” filter_color=”grey” filter_size=”md” button_style=”rounded” button_color=”blue” button_size=”md” arrows_design=”none” arrows_position=”inside” arrows_color=”blue” paging_design=”radio_dots” paging_color=”grey” loop=”” autoplay=”-1″ item=”4123″ taxonomies=”72″][\/vc_column][\/vc_row][vc_row][vc_column width=”2\/3″][vc_column_text]At NEB, enzyme production is linked to basic research in the cloning and overexpression of restriction-modification systems. This focus allows us to provide extremely pure enzymes at concentrations that deliver more flexibility to your experimental design.<\/p>\n

Whether you are quickly screening large numbers of clones or setting up overnight digests, you will benefit from the high quality of our enzymes. Typically, a restriction digest involves the incubation of 1 \u00b5l of enzyme with 1 \u00b5g of purified DNA in a final volume of 50 \u00b5l for 1 hour. However, to speed up the screening process, choose a Time-Saver\u2122 qualified enzyme for 5-15 minute digestion reactions. These enzymes will digest 1 \u00b5g of substrate DNA in 5-15 minutes using 1 \u00b5l of enzyme under recommended reaction conditions, and can also be used safely in overnight digestions. Unlike other suppliers, there is no special formulation, change in concentration or need to buy more expensive new lines of enzymes to achieve digestion in 5-15 minutes, nor do you have to worry if you incubate too long.[\/vc_column_text][\/vc_column][vc_column width=”1\/3″][vc_column_text]Advantages<\/strong><\/p>\n

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