{"id":18755,"date":"2019-05-09T17:03:43","date_gmt":"2019-05-09T15:03:43","guid":{"rendered":"https:\/\/www.neb-online.de\/?page_id=18755"},"modified":"2025-06-23T17:10:17","modified_gmt":"2025-06-23T15:10:17","slug":"the-new-nebnext-enzymatic-methyl-seq-em-seq","status":"publish","type":"page","link":"https:\/\/www.neb-online.de\/en\/next-generation-sequencing\/the-new-nebnext-enzymatic-methyl-seq-em-seq\/","title":{"rendered":"The new NEBNext Enzymatic Methyl-seq (EM-seq)"},"content":{"rendered":"

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A high-performance alternative to bisulfite sequencing for methylome analysis<\/h2>\n

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Whole Genome Bisulfite Sequencing (WGBS) has long been the gold standard for methylome analysis, but the chemical bisulfite reaction damages and degrades DNA, resulting in fragmentation and loss. Additionally, bisulfite libraries demonstrate significant GC bias and are enriched for methylated regions.
\nTo overcome these limitations, we developed an enzyme-based approach, NEBNext Enzymatic Methyl-seq (EM-seq), a new method for identification of 5mC and 5hmC.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n

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