{"id":20471,"date":"2019-10-23T15:52:51","date_gmt":"2019-10-23T13:52:51","guid":{"rendered":"https:\/\/www.neb-online.de\/?page_id=20471"},"modified":"2022-04-27T15:44:35","modified_gmt":"2022-04-27T13:44:35","slug":"nebnext-direct-genotyping-solution-for-illumina","status":"publish","type":"page","link":"https:\/\/www.neb-online.de\/en\/nebnext-direct-genotyping-solution-for-illumina\/","title":{"rendered":"NEBNext Direct Genotyping Solution for Illumina"},"content":{"rendered":"

[vc_row][vc_column][vc_basic_grid post_type=”post” max_items=”3″ element_width=”12″ item=”4123″ grid_id=”vc_gid:1651067055178-8ade9c55-e145-2″ taxonomies=”128″][\/vc_column][\/vc_row][vc_row][vc_column width=”1\/2″][vc_column_text]<\/p>\n

Your Genotyping Solution<\/h2>\n

The NEBNext Direct Genotyping Solution combines highly multiplexed, capture-based enrichment with maximum efficiency next generation sequencing to deliver cost-effective, high-throughput genotyping for a wide variety of applications.[\/vc_column_text][vc_column_text]Applicable for ranges spanning 100-5,000 markers, pre-capture multiplexing of up to 96 samples combined with 8 standard pool-indexes allows analysis of up to 768 samples \/ 3.8 million genotypes in a single Illumina sequencing run.<\/p>\n

More pool-indexes are available upon request, allowing for multiplexing of up to 9,160 samples in a single run.[\/vc_column_text][\/vc_column][vc_column width=”1\/2″][vc_column_text]<\/p>\n\n\n\n\n\n\n
\"Genotyping<\/strong><\/td>\n\"Genotyping<\/strong><\/td>\n\"Genotyping<\/strong><\/td>\n<\/tr>\n
Plant<\/strong><\/span><\/td>\nAnimal<\/strong><\/span><\/td>\nHuman<\/strong><\/span><\/td>\n<\/tr>\n
Marker Assisted Selection\/ Breeding<\/span><\/td>\nGenotyping<\/span><\/td>\nBiobanking<\/span><\/td>\n<\/tr>\n
QTL Screening<\/span><\/td>\nLivestock Breeding<\/span><\/td>\nNGS Sample Tracking<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

[\/vc_column_text][vc_separator][vc_column_text css=”.vc_custom_1604315839371{margin-bottom: 0px !important;border-bottom-width: 0px !important;padding-bottom: 10px !important;}”]<\/p>\n

Highly multiplexed capture of genomic markers for high throughput NGS based genotyping<\/strong><\/p>\n

[\/vc_column_text][vc_single_image image=”25429″ img_size=”large” onclick=”custom_link” link=”https:\/\/www.neb.com\/en\/tools-and-resources\/video-library\/highly-multiplexed-capture-of-genomic-markers-for-high-throughput-ngs-based-genotyping”]Watch now!<\/a>[\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n

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[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][\/vc_column][\/vc_row][vc_row bwd_tbc4vd_show_weekday=”” css=”.vc_custom_1571321840152{margin-top: 20px !important;margin-bottom: 20px !important;}”][vc_column][vc_column_text css=”.vc_custom_1571837862247{border-top-width: 2px !important;border-bottom-width: 2px !important;padding-top: 10px !important;padding-right: 10px !important;padding-bottom: 10px !important;padding-left: 10px !important;background-color: #f4f1e7 !important;border-top-color: #eb7e2d !important;border-top-style: solid !important;border-bottom-color: #eb7e2d !important;border-bottom-style: solid !important;}”]NEB will develop and balance bait sets using purified genomic DNA from your source material. This allows us to optimize performance for your specific application, with the quality of DNA that is typically obtained.<\/strong><\/p>\n

To discuss your application, please use our contact form<\/a> or email nebnextdirect@neb.com<\/a> .<\/strong>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n

NEBNext Direct Genotyping Solution Workflow<\/h2>\n

The NEBNext Direct Genotyping Solution begins with 25-100 ng of purified genomic DNA. The DNA molecules are enzymatically fragmented and 5 \u0301 tagged with an Illumina-compatible P5 adaptors, incorporating both an inline sample index to tag each sample prior to pooling and an inline Unique Molecular Identifier (UMI) to mark each unique DNA fragment within the samples.[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=”1\/2″][vc_column_text]Up to 96 samples are subsequently pooled together prior to hybridization-based enrichment using biotinylated baits and captured on streptavidin beads. For the remainder of the protocol, up to 96 samples are processed as a single pool through ligation of a 3 \u0301 adaptor, removal of off-target sequence and final PCR, which amplifies the material and adds a second pool index to produce the final sequencing-ready fragment.[\/vc_column_text][\/vc_column][vc_column width=”1\/2″]

\n\t\t\t\tAdvantages:<\/span>\n\t\t\t\t\t