{"id":2066,"date":"2015-04-27T10:38:34","date_gmt":"2015-04-27T10:38:34","guid":{"rendered":"http:\/\/www.neb-online.de\/?page_id=2066"},"modified":"2023-10-13T15:49:56","modified_gmt":"2023-10-13T13:49:56","slug":"dna-assembly","status":"publish","type":"page","link":"https:\/\/www.neb-online.de\/en\/cloning-synthetic-biology\/dna-assembly\/","title":{"rendered":"DNA Assembly"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column][vc_basic_grid post_type=&#8221;post&#8221; max_items=&#8221;10&#8243; element_width=&#8221;12&#8243; gap=&#8221;15&#8243; item=&#8221;4123&#8243; taxonomies=&#8221;72&#8243; grid_id=&#8221;vc_gid:1697204887800-95ff1dc3-c4eb-5&#8243;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n<h2>\u201eCloning 2.0\u201c \u2013\u00a0DNA Assembly and cloning products for synthetic biology<\/h2>\n<p>[\/vc_column_text][vc_column_text]The field of synthetic biology is steadily gaining popularity, not only because of the international iGEM competition which was supported by NEB recently.<\/p>\n<p>Due to the high interest in synthetic biology, new cloning and gene editing methods were developed. These methods allow to\u00a0create large, artificial DNA structures through re-organisation, editing or assembly of genes.<\/p>\n<p>NEB offers a variety of innovative\u00a0\u201eCloning 2.0\u201c products to simplify your daily cloning workflows.[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][\/vc_column][\/vc_row][vc_row bwd_tbc4vd_show_weekday=&#8221;&#8221; css=&#8221;.vc_custom_1697204995180{border-top-width: 1px !important;border-right-width: 1px !important;border-bottom-width: 1px !important;border-left-width: 1px !important;border-left-color: #e0e0e0 !important;border-left-style: solid !important;border-right-color: #e0e0e0 !important;border-right-style: solid !important;border-top-color: #e0e0e0 !important;border-top-style: solid !important;border-bottom-color: #e0e0e0 !important;border-bottom-style: solid !important;border-radius: 1px !important;}&#8221;][vc_column]<div id=\"shorten-65626\" class=\"shorten\" 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Available with\u00a0(<a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=E5520\" target=\"_blank\" rel=\"noopener\">NEB #E5520<\/a>)\u00a0and without\u00a0(<a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=E2621\" target=\"_blank\" rel=\"noopener\">NEB #E2621<\/a>)\u00a0competent\u00a0E. coli, this flexible kit enables simple and fast Seamless Cloning utilizing a new proprietary high-fidelity polymerase. Find out why NEBuilder HiFi is the next generation of DNA assembly and cloning.[\/vc_column_text]<a class=\"btn btn-standard form-btn\"  href=\"https:\/\/www.neb-online.de\/en\/cloning-synthetic-biology\/dna-assembly\/nebuilder-dna-assembly\/\" style=\"margin-left:auto;margin-right:auto;\">Learn more...<\/a>[\/vc_column][vc_column width=&#8221;1\/2&#8243; css=&#8221;.vc_custom_1673442719205{border-top-width: 5px !important;border-right-width: 5px !important;border-right-color: #ffffff !important;border-right-style: solid !important;border-top-color: #ffffff !important;border-top-style: solid !important;}&#8221;][vc_single_image image=&#8221;8757&#8243; img_size=&#8221;full&#8221;][vc_column_text]<\/p>\n<h2>NEBridge Golden Gate Assembly Kit<\/h2>\n<p>[\/vc_column_text][vc_column_text]The efficient and seamless assembly of DNA fragments, commonly referred to as <a href=\"https:\/\/www.neb-online.de\/en\/art\/E1601\" target=\"_blank\" rel=\"noopener\">NEBridge Golden Gate Assembly Kit<\/a>, has its origins in 1996, when for the first time it was shown that multiple inserts could be assembled into a vector backbone using only the sequential or simultaneous activities of a single type IIS <a title=\"Restriction Enzymes\" href=\"https:\/\/www.neb-online.de\/en\/cloning-synthetic-biology\/restriktionsenzyme\/\">restriction enzyme<\/a> and <a title=\"T4 DNA Ligase\" href=\"https:\/\/www.neb-online.de\/en\/cloning-synthetic-biology\/dna-ligases\/\">T4 DNA ligase<\/a>.[\/vc_column_text]<a class=\"btn btn-standard form-btn\"  href=\"https:\/\/www.neb-online.de\/en\/cloning-synthetic-biology\/dna-assembly\/golden-gate-assembly\/\" style=\"margin-left:auto;margin-right:auto;\">Learn more...<\/a>[\/vc_column][\/vc_row][vc_row bwd_tbc4vd_show_weekday=&#8221;&#8221; equal_height=&#8221;yes&#8221; css=&#8221;.vc_custom_1673442601733{margin-right: -1px !important;margin-left: -1px !important;background-color: #f3f3f3 !important;}&#8221;][vc_column width=&#8221;1\/2&#8243; css=&#8221;.vc_custom_1673442697978{border-top-width: 5px !important;border-right-width: 5px !important;border-right-color: #ffffff !important;border-right-style: solid !important;border-top-color: #ffffff !important;border-top-style: solid !important;}&#8221;][vc_single_image image=&#8221;40790&#8243; img_size=&#8221;full&#8221;][vc_column_text]<\/p>\n<h2>CRISPR \/ Cas9 \u2013 Genome Editing<\/h2>\n<p>The targeted modification of the genome in living cells &#8211; made possible by the development of new molecular DNA cutting techniques. Currently, the best known and most discussed technique is based on the bacterial CRISPR-associated protein-9 (Cas9) nuclease from Streptococcus pyogenes.[\/vc_column_text]<a class=\"btn btn-standard form-btn\"  href=\"https:\/\/www.neb-online.de\/en\/genome-editing\/\" style=\"margin-left:auto;margin-right:auto;\">Learn more...<\/a>[\/vc_column][vc_column width=&#8221;1\/2&#8243; css=&#8221;.vc_custom_1673442719205{border-top-width: 5px !important;border-right-width: 5px !important;border-right-color: #ffffff !important;border-right-style: solid !important;border-top-color: #ffffff !important;border-top-style: solid !important;}&#8221;][vc_single_image image=&#8221;40920&#8243; img_size=&#8221;full&#8221;][vc_column_text]<\/p>\n<h2>Q5 High-Fidelity DNA Polymerase<\/h2>\n<p>[\/vc_column_text][vc_column_text]NEB&#8217;s Q5 High-Fidelity DNA Polymerase sets the industry standard in PCR and combines extreme accuracy (&gt;280\u00d7 more accurate than Taq) with highest performance! Additionally, thanks to short elongation times and PCR protocols, the Q5 Polymerase saves you valuable lab time.[\/vc_column_text]<a class=\"btn btn-standard form-btn\"  href=\"https:\/\/www.neb-online.de\/en\/cloning-synthetic-biology\/dna-assembly\/golden-gate-assembly\/\" style=\"margin-left:auto;margin-right:auto;\">Learn more...<\/a>[\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row][vc_column][vc_basic_grid post_type=&#8221;post&#8221; max_items=&#8221;10&#8243; element_width=&#8221;12&#8243; gap=&#8221;15&#8243; item=&#8221;4123&#8243; taxonomies=&#8221;72&#8243; grid_id=&#8221;vc_gid:1697204887800-95ff1dc3-c4eb-5&#8243;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text] \u201eCloning 2.0\u201c \u2013\u00a0DNA Assembly and cloning products for synthetic biology [\/vc_column_text][vc_column_text]The field of synthetic biology is steadily gaining popularity, not only because of the international iGEM competition which was supported by NEB recently. Due to the high interest in synthetic biology, new cloning and gene editing methods&#8230;  <a class=\"excerpt-read-more\" href=\"https:\/\/www.neb-online.de\/en\/cloning-synthetic-biology\/dna-assembly\/\" title=\"Read DNA Assembly\">Read more &raquo;<\/a><\/p>\n","protected":false},"author":2,"featured_media":49248,"parent":1824,"menu_order":0,"comment_status":"open","ping_status":"closed","template":"page-sidebar.php","meta":{"footnotes":""},"class_list":["post-2066","page","type-page","status-publish","has-post-thumbnail","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.1.1 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>DNA Assembly - New England Biolabs GmbH<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.neb-online.de\/en\/cloning-synthetic-biology\/dna-assembly\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"DNA Assembly - New England Biolabs GmbH\" \/>\n<meta property=\"og:description\" content=\"[vc_row][vc_column][vc_basic_grid post_type=&#8221;post&#8221; max_items=&#8221;10&#8243; element_width=&#8221;12&#8243; gap=&#8221;15&#8243; item=&#8221;4123&#8243; taxonomies=&#8221;72&#8243; grid_id=&#8221;vc_gid:1697204887800-95ff1dc3-c4eb-5&#8243;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text] \u201eCloning 2.0\u201c \u2013\u00a0DNA Assembly and cloning products for synthetic biology [\/vc_column_text][vc_column_text]The field of synthetic biology is steadily gaining popularity, not only because of the international iGEM competition which was supported by NEB recently. 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