{"id":2109,"date":"2015-04-07T19:29:58","date_gmt":"2015-04-07T19:29:58","guid":{"rendered":"http:\/\/www.neb-online.de\/?page_id=2109"},"modified":"2025-11-19T09:36:46","modified_gmt":"2025-11-19T08:36:46","slug":"q5-high-fidelity-dna-polymerase","status":"publish","type":"page","link":"https:\/\/www.neb-online.de\/en\/pcr-and-dna-amplification\/high-fidelity-pcr\/q5-high-fidelity-dna-polymerase\/","title":{"rendered":"Q5 High Fidelity DNA Polymerase"},"content":{"rendered":"

[vc_row][vc_column][vc_basic_grid post_type=”post” max_items=”3″ element_width=”12″ gap=”15″ item=”4123″ taxonomies=”70″ grid_id=”vc_gid:1763541289306-9edf8f80-65cd-5″][\/vc_column][\/vc_row][vc_row bwd_show_when_type=”before” bwd_show_when_date=”10\/10\/2022 00:00″ bwd_tbc4vd_show_weekday=””][vc_column width=”1\/2″ css=”.vc_custom_1429279657733{margin-bottom: 20px !important;}”][vc_column_text]<\/p>\n

A new standard for High Fidelity PCR<\/h2>\n

Q5 High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance.<\/p>\n

With the highest fidelity amplification available (~280 times higher than\u00a0Taq<\/em>), Q5 DNA Polymerase results in ultra-low error rates, even lower than Phusion Polymerase[\/vc_column_text][vc_column_text]<\/p>\n

Engineered for Performance<\/h2>\n

Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.<\/p>\n

Q5 High-Fidelity DNA Polymerase is supplied with an optimized buffer system that allows robust amplification regardless of GC content. The 5X Q5 Reaction Buffer contains 2 mM Mg++ at final (1X) reaction concentrations and is recommended for most routine applications. For GC-rich targets (\u2265 65% GC), amplification can be improved by the addition of the 5X Q5 High GC Enhancer.[\/vc_column_text][vc_column_text]Q5 DNA Polymerase is also available as a hot start version. The Q5 Hot Start DNA Polymerase<\/strong> utilizes NEBs aptamer technology, allowing room temperature reaction setup without the need of an additional activation step.[\/vc_column_text][\/vc_column][vc_column width=”1\/2″][vc_text_separator title=”Video: Q5 High-Fidelity Polymerase” color=”custom” accent_color=”#eb7e2d”][vc_video link=”https:\/\/youtu.be\/-UP5A08UOQE” align=”center”][vc_separator color=”custom” accent_color=”#eb7e2d”][vc_single_image image=”42059″ img_size=”full”][\/vc_column][\/vc_row][vc_row bwd_show_when_type=”after” bwd_show_when_date=”10\/10\/2022 00:00″ bwd_tbc4vd_show_weekday=”” disable_element=”yes”][vc_column width=”1\/2″ css=”.vc_custom_1429279657733{margin-bottom: 20px !important;}”][vc_column_text]<\/p>\n

A new standard for High Fidelity PCR<\/h2>\n

Q5 High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance.<\/p>\n

With the highest fidelity amplification available (~280 times higher than\u00a0Taq<\/em>), Q5 DNA Polymerase results in ultra-low error rates, even lower than Phusion Polymerase[\/vc_column_text][vc_column_text]<\/p>\n

Engineered for Performance<\/h2>\n

Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.<\/p>\n

Q5 High-Fidelity DNA Polymerase is supplied with an optimized buffer system that allows robust amplification regardless of GC content. The 5X Q5 Reaction Buffer contains 2 mM Mg++ at final (1X) reaction concentrations and is recommended for most routine applications. For GC-rich targets (\u2265 65% GC), amplification can be improved by the addition of the 5X Q5 High GC Enhancer.[\/vc_column_text][vc_column_text]Q5 DNA Polymerase is also available as a hot start version. The Q5 Hot Start DNA Polymerase<\/strong> utilizes NEBs aptamer technology, allowing room temperature reaction setup without the need of an additional activation step.[\/vc_column_text][\/vc_column][vc_column width=”1\/2″][vc_single_image image=”43597″ img_size=”medium” alignment=”center” onclick=”custom_link” css=”.vc_custom_1663854605704{margin-bottom: 0px !important;border-bottom-width: 0px !important;padding-bottom: 0px !important;}” link=”https:\/\/www.neb-online.de\/en\/10-jahre-unerreichte-pcr-performance\/”][vc_column_text css=”.vc_custom_1663854616872{margin-top: 0px !important;border-top-width: 0px !important;padding-top: 0px !important;}”]<\/p>\n

Please click here to learn more about the<\/span><\/a>
\n20 % Discount<\/strong><\/span><\/p>\n

[\/vc_column_text][vc_text_separator title=”Video: Q5 High-Fidelity Polymerase” color=”custom” accent_color=”#eb7e2d”][vc_video link=”https:\/\/youtu.be\/-UP5A08UOQE” align=”center”][vc_separator color=”custom” accent_color=”#eb7e2d”][vc_single_image image=”42059″ img_size=”full”][\/vc_column][\/vc_row][vc_row bwd_show_when_type=”before” bwd_show_when_date=”10\/10\/2022 00:00″ bwd_tbc4vd_show_weekday=””][vc_column][vc_column_text]Q5 DNA Polymerase is also available as a hot start version. The Q5 Hot Start DNA Polymerase<\/strong> utilizes NEBs aptamer technology, allowing room temperature reaction setup without the need of an additional activation step.[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=”custom” accent_color=”#eb7e2d”][vc_column_text]<\/p>\n

Get a free Sample of Q5 High Fidelity Polymerase!<\/h2>\n

[\/vc_column_text]Sample form<\/a>[vc_separator color=”custom” accent_color=”#eb7e2d”][\/vc_column][\/vc_row][vc_row][vc_column width=”1\/2″][vc_column_text]<\/p>\n

Five Quality Features of Q5<\/h2>\n

Q1 Fidelity<\/h3>\n

The highest fidelity amplification available (~280X higher than Taq, ~7x higher than Phusion Polymerase)”Ultra-Fidelity” (~280X higher than Taq, ~7x higher than Phusion Polymerase)<\/p>\n

Q2 Robustness<\/h3>\n

High specificity and yield with minimal optimization<\/p>\n

Q3 Coverage<\/h3>\n

Superior performance for a broad range of amplicons (from high AT to high GC)<\/p>\n

Q4 Speed<\/h3>\n

Short extension times<\/p>\n

Q5 Amplicon length<\/h3>\n

Robust amplifications up to 20 kb for simple templates, and 10 kb for complex[\/vc_column_text][\/vc_column][vc_column width=”1\/2″][vc_text_separator title=”Video: Important tips for Q5 Polymerase” color=”custom” accent_color=”#eb7e2d”][vc_video link=”https:\/\/youtu.be\/ity1fhL4uaM”][vc_separator color=”custom” accent_color=”#eb7e2d”][vc_row_inner][vc_column_inner width=”2\/3″][vc_column_text]<\/p>\n

Feature Article:<\/strong><\/span><\/p>\n

\u201ePolymerase Fidelity \u2013
\nWhat is it and what does it mean for your PCR?\u201c<\/strong><\/p>\n

[\/vc_column_text][\/vc_column_inner][vc_column_inner width=”1\/3″][vc_single_image image=”41765″ img_size=”120×160″ onclick=”custom_link” img_link_target=”_blank” link=”https:\/\/www.neb-online.de\/wp-content\/uploads\/2018\/06\/Polymerase-Fidelity-What-is-it-and-what-does-it-mean-for-your-PCR.pdf”][\/vc_column_inner][\/vc_row_inner]Download PDF<\/a>[\/vc_column][\/vc_row][vc_row bwd_tbc4vd_show_weekday=”” disable_element=”yes”][vc_column][vc_separator color=”custom” accent_color=”#eb7e2d”][\/vc_column][\/vc_row][vc_row bwd_tbc4vd_show_weekday=”” disable_element=”yes”][vc_column]

[vc_column_text]<\/p>\n

Q5U Hot Start High-Fidelity DNA Polymerase<\/h2>\n

[\/vc_column_text][vc_row_inner][vc_column_inner width=”1\/2″][vc_column_text]Q5U is a modified version of Q5 High-Fidelity DNA Polymerase containing a mutation in the uracil-binding pocket that enables the ability to read and amplify templates containing uracil and inosine bases.<\/p>\n

This is useful for amplifying bisulfite-converted, enzymatically-deaminated, or damaged DNA, preventing carryover contamination in PCR (when used with dUTP and UDG), and in USER cloning methods.<\/p>\n

Optimized protocols are available for recommended applications.<\/strong>[\/vc_column_text][\/vc_column_inner][vc_column_inner width=”1\/2″][vc_single_image image=”42060″ img_size=”full”][\/vc_column_inner][\/vc_row_inner]<\/div>Read more<\/a>