{"id":21223,"date":"2020-01-02T13:30:01","date_gmt":"2020-01-02T12:30:01","guid":{"rendered":"https:\/\/www.neb-online.de\/pcr-and-dna-amplification\/qpcr-real-time-pcr-and-rt-qpcr\/luna-cell-ready\/"},"modified":"2023-12-19T15:19:49","modified_gmt":"2023-12-19T14:19:49","slug":"luna-cell-ready","status":"publish","type":"page","link":"https:\/\/www.neb-online.de\/en\/pcr-and-dna-amplification\/qpcr-real-time-pcr-and-rt-qpcr\/luna-cell-ready\/","title":{"rendered":"Luna Cell Ready One-Step RT-qPCR Kits"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column][vc_row_inner][vc_column_inner width=&#8221;2\/3&#8243;][vc_separator color=&#8221;white&#8221;][vc_column_text]<\/p>\n<h3><strong>Luna Cell Ready One-Step RT qPCR Kits \u2013\u00a0<\/strong><\/h3>\n<h2><strong><span style=\"color: #f58025;\">Go direct from cells to RNA quantitation without purification<\/span><\/strong><\/h2>\n<p>The Luna Cell Ready One-Step RT-qPCR Kit provides all the necessary components for direct, dye-based RNA detection and quantitation, bypassing the need for RNA extraction and purification.<\/p>\n<p>Cell cultures are often analyzed for gene expression or treatment responses as a proxy for a living organism. The Luna Cell Ready One-Step RT-qPCR Kit offers an alternative workflow resulting in effective cell lysis, RNA release, and genomic DNA removal simultaneously in a 15-minute protocol. The Lysis Module includes a unique Luna Cell Ready RNA Protection Reagent that maintains RNA integrity during cell lysis.[\/vc_column_text][\/vc_column_inner][vc_column_inner width=&#8221;1\/3&#8243;][vc_separator color=&#8221;white&#8221;]<div class=\"vbox\">\n\t\t\t\t<span class=\"vhead\">Advantages:<\/span>\n\t\t\t\t\t<ul class=\"vlist\">\n\t\t\t\t\t\t<li><span>Contains reagents for 100 lysis preps and 500 one-step RT-qPCR reactions (dye)<br \/><\/span><\/li><li><span>Effective cell lysis preparation from 10 to 100,000 cells across numerous cell lines<br \/><\/span><\/li><li><span>Coordinated cell lysis, RNA release, and genomic DNA removal in a fast 15-minute protocol<br \/><\/span><\/li><li><span>Features Luna WarmStart RT paired with Hot Start Taq for increased thermostability and room temperature setup<br \/><\/span><\/li><li><span>Additional lysis preps available: Luna Cell Ready Lysis Module (NEB #E3032)<\/span><\/li>\n\t\t\t\t\t<\/ul>\n\t\t\t<\/div>[\/vc_column_inner][\/vc_row_inner][vc_row_inner][vc_column_inner][vc_separator color=&#8221;white&#8221;][\/vc_column_inner][\/vc_row_inner][vc_row_inner][vc_column_inner width=&#8221;2\/3&#8243;][vc_single_image image=&#8221;21129&#8243; img_size=&#8221;full&#8221;][\/vc_column_inner][vc_column_inner width=&#8221;1\/3&#8243;][vc_column_text]<em><span style=\"font-size: 10px;\">By coordinating the actions of DNase I and the Luna Cell Ready Protease, the Luna Cell Ready Lysis Module offers a simple workflow resulting in effective cell lysis, RNA release and genomic DNA removal simultaneously in a 15-minute protocol. Up to 2 \u00b5l lysate (equivalent to RNA from 0.2 &#8211; 4,000 cells) can be transferred into 20 \u00b5l downstream RT-qPCR reactions.<br \/>\n<\/span><\/em>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner][vc_column_inner][vc_separator][\/vc_column_inner][\/vc_row_inner][vc_row_inner][vc_column_inner width=&#8221;2\/3&#8243;][vc_single_image image=&#8221;21130&#8243; img_size=&#8221;full&#8221;][\/vc_column_inner][vc_column_inner width=&#8221;1\/3&#8243;][vc_column_text]<em><span style=\"font-size: 10px;\"> The Luna Cell Ready One-Step RT-qPCR Kit offers reliable and precise RNA quantitation comparable to purified RNA across 5-log cell inputs.<\/span><\/em><\/p>\n<p><em><span style=\"font-size: 10px;\">Detection of \u03b2-actin, an abundant target, and ARF3 and Tubulin, two less abundant targets, across 5-logs of cell inputs. Efficiency (E) for each target is shown at the lower left corners of the panel. B. Cq relative to \u03b2-actin at each dilution (\u0394Cq (GOI)) was calculated from the data in (A) across 5-log cell inputs. The average Cqs are shown as bars<br \/>\n<\/span><\/em>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner][vc_column_inner][vc_separator][\/vc_column_inner][\/vc_row_inner][vc_row_inner bwd_show_when_type=&#8221;before&#8221; bwd_show_when_date=&#8221;12\/31\/2023 23:00&#8243; bwd_tbc4vd_show_weekday=&#8221;&#8221;][vc_column_inner][vc_column_text]The new Luna Cell Ready One-Step RT-qPCR Kits make the quantification of RNA faster and easier than ever before.<\/p>\n<table style=\"width: 760px;\" border=\"0\" cellpadding=\"5\">\n<tbody>\n<tr style=\"background-color: #f3f3f3;\">\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"400\" height=\"40px\">PRODUCT<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"88\">Prod.Nr.:<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"100\">SIZE<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" colspan=\"3\">PRICE<\/th>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Luna Cell Ready One-Step RT-qPCR Kit<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E3030S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 + 500 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>996 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/E3030\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb.com\/en\/E3030\" target=\"blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"NEB Info_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Luna Cell Ready Probe One-Step RT-qPCR Kit<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E3031S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 + 500 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>946 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/E3031\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb.com\/en\/E3031\" target=\"blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"NEB Info_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Luna Cell Ready Lysis Module<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E3032S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>496 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/E3032\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb.com\/en\/E3032\" target=\"blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"NEB Info_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p style=\"text-align: right;\"><em><span class=\"bildunterschrift\">As of: 01.01.2023<\/span><\/em><\/p>\n<p>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner bwd_show_when_type=&#8221;after&#8221; bwd_show_when_date=&#8221;12\/31\/2023 23:00&#8243; bwd_tbc4vd_show_weekday=&#8221;&#8221;][vc_column_inner][vc_column_text]The new Luna Cell Ready One-Step RT-qPCR Kits make the quantification of RNA faster and easier than ever before.<\/p>\n<table style=\"width: 760px;\" border=\"0\" cellpadding=\"5\">\n<tbody>\n<tr style=\"background-color: #f3f3f3;\">\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"400\" height=\"40px\">PRODUCT<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"88\">Prod.Nr.:<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"100\">SIZE<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" colspan=\"2\">INFO<\/th>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Luna Cell Ready One-Step RT-qPCR Kit<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E3030S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 + 500 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/E3030\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb.com\/en\/E3030\" target=\"blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"NEB Info_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Luna Cell Ready Probe One-Step RT-qPCR Kit<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E3031S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 + 500 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/E3031\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb.com\/en\/E3031\" target=\"blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"NEB Info_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Luna Cell Ready Lysis Module<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E3032S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/E3032\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb.com\/en\/E3032\" target=\"blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"NEB Info_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p style=\"text-align: right;\"><em><span class=\"bildunterschrift\">As of: 01.01.2024<\/span><\/em><\/p>\n<p>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row][vc_column][vc_row_inner][vc_column_inner width=&#8221;2\/3&#8243;][vc_separator color=&#8221;white&#8221;][vc_column_text] Luna Cell Ready One-Step RT qPCR Kits \u2013\u00a0 Go direct from cells to RNA quantitation without purification The Luna Cell Ready One-Step RT-qPCR Kit provides all the necessary components for direct, dye-based RNA detection and quantitation, bypassing the need for RNA extraction and purification. Cell cultures are often analyzed for gene expression&#8230;  <a class=\"excerpt-read-more\" href=\"https:\/\/www.neb-online.de\/en\/pcr-and-dna-amplification\/qpcr-real-time-pcr-and-rt-qpcr\/luna-cell-ready\/\" title=\"Read Luna Cell Ready One-Step RT-qPCR Kits\">Read more &raquo;<\/a><\/p>\n","protected":false},"author":18,"featured_media":24399,"parent":18208,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-sidebar.php","meta":{"footnotes":""},"class_list":["post-21223","page","type-page","status-publish","has-post-thumbnail","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.1.1 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Luna Cell Ready One-Step RT-qPCR Kits - New England Biolabs GmbH<\/title>\n<meta name=\"robots\" content=\"noindex, nofollow\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Luna Cell Ready One-Step RT-qPCR Kits - New England Biolabs GmbH\" \/>\n<meta property=\"og:description\" content=\"[vc_row][vc_column][vc_row_inner][vc_column_inner width=&#8221;2\/3&#8243;][vc_separator color=&#8221;white&#8221;][vc_column_text] Luna Cell Ready One-Step RT qPCR Kits \u2013\u00a0 Go direct from cells to RNA quantitation without purification The Luna Cell Ready One-Step RT-qPCR Kit provides all the necessary components for direct, dye-based RNA detection and quantitation, bypassing the need for RNA extraction and purification. Cell cultures are often analyzed for gene expression... 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