{"id":2141,"date":"2015-04-27T14:15:01","date_gmt":"2015-04-27T14:15:01","guid":{"rendered":"http:\/\/www.neb-online.de\/?page_id=2141"},"modified":"2023-12-19T15:43:42","modified_gmt":"2023-12-19T14:43:42","slug":"isothermal-amplification","status":"publish","type":"page","link":"https:\/\/www.neb-online.de\/en\/pcr-and-dna-amplification\/isothermal-amplification\/","title":{"rendered":"Isothermal Amplification"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column][vc_basic_grid post_type=&#8221;post&#8221; max_items=&#8221;10&#8243; element_width=&#8221;12&#8243; gap=&#8221;15&#8243; item=&#8221;4123&#8243; taxonomies=&#8221;70&#8243; grid_id=&#8221;vc_gid:1702996944067-a743b33f-0a68-5&#8243;][\/vc_column][\/vc_row][vc_row css=&#8221;.vc_custom_1448619604073{margin-right: 5px !important;}&#8221;][vc_column][vc_row_inner][vc_column_inner width=&#8221;2\/3&#8243;][vc_column_text]<strong>Isothermal Amplification is becoming more and more popular as a simple and cost-efficient alternative to standard PCR.<\/strong>[\/vc_column_text][vc_column_text]<\/p>\n<h2><span style=\"color: #f58025;\">How does LAMP (Loop-mediated isothermal amplification) work?<\/span><\/h2>\n<p>Loop-mediated isothermal amplification (LAMP) uses 4-6 primers to amplify a specific DNA fragment at a constant temperature. A strand-displacing DNA polymerase initiates synthesis and two specially designed primers form loop structures to facilitate subsequent rounds of amplification through extension on the loops and additional annealing of primers.[\/vc_column_text][\/vc_column_inner][vc_column_inner width=&#8221;1\/3&#8243;]<div class=\"vbox\">\n\t\t\t\t<span class=\"vhead\">Advantages:<\/span>\n\t\t\t\t\t<ul class=\"vlist\">\n\t\t\t\t\t\t<li><span>No PCR Cycler necessary<br \/><\/span><\/li><li><span>Fast protocols<br \/><\/span><\/li><li><span>High reliability &amp; specificity<\/span><\/li>\n\t\t\t\t\t<\/ul>\n\t\t\t<\/div>[\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row css=&#8221;.vc_custom_1448617723904{margin-top: 50px !important;margin-bottom: 50px !important;}&#8221;][vc_column][vc_column_text]<\/p>\n<h2><span style=\"color: #f58025;\">Schematic illustration LAMP, Featured Article &amp; Videos<\/span><\/h2>\n<p>[\/vc_column_text][vc_row_inner][vc_column_inner width=&#8221;1\/2&#8243;][vc_single_image image=&#8221;7158&#8243; img_size=&#8221;full&#8221; alignment=&#8221;center&#8221; style=&#8221;vc_box_shadow&#8221; img_link_large=&#8221;yes&#8221;][\/vc_column_inner][vc_column_inner width=&#8221;1\/4&#8243;][vc_single_image image=&#8221;39383&#8243; img_size=&#8221;full&#8221; alignment=&#8221;center&#8221; onclick=&#8221;custom_link&#8221; img_link_target=&#8221;_blank&#8221; link=&#8221;https:\/\/www.medrxiv.org\/content\/10.1101\/2020.02.26.20028373v1.full.pdf&#8221;]<a class=\"btn btn-standard form-btn\" target=\"_blank\" href=\"https:\/\/www.neb-online.de\/literatur\/Article\/yNEB560_EuroExpressions_Fall_2020_Article.pdf\" style=\"margin-right:auto;\">Download PDF here<\/a>[\/vc_column_inner][vc_column_inner width=&#8221;1\/4&#8243;][\/vc_column_inner][\/vc_row_inner][vc_separator color=&#8221;black&#8221;][vc_row_inner][vc_column_inner width=&#8221;1\/2&#8243;][vc_video link=&#8221;https:\/\/youtu.be\/L5zi2P4lggw&#8221;][\/vc_column_inner][vc_column_inner width=&#8221;1\/2&#8243;][vc_column_text]Did you know that this method of Isothermal Amplification can be performed in only 5-10 minutes without sophisticated technical equipment like a PCR cycler or similar devices?<\/p>\n<p>Learn more about this method in our new video-tutorial <strong>\u201cOverview of Loop-mediated isothermal Amplification\u201d<\/strong>.[\/vc_column_text]<a class=\"btn btn-standard form-btn\" target=\"_blank\" href=\"https:\/\/youtu.be\/L5zi2P4lggw\" style=\"margin-right:auto;\">Watch this video<\/a>[\/vc_column_inner][\/vc_row_inner][vc_row_inner][vc_column_inner width=&#8221;1\/2&#8243;][vc_video link=&#8221;https:\/\/youtu.be\/9Kv_hSDEX7M&#8221;][\/vc_column_inner][vc_column_inner width=&#8221;1\/2&#8243;][vc_column_text]NEB Scientists have recently published a well respected paper on highly-specific detection of pathogens of the tropical disease &#8220;river blindness&#8221; through LAMP and a simple color change.[\/vc_column_text]<a class=\"btn btn-standard form-btn\" target=\"_blank\" href=\"https:\/\/youtu.be\/9Kv_hSDEX7M\" style=\"margin-right:auto;\">Watch this video<\/a>[\/vc_column_inner][\/vc_row_inner][vc_row_inner][vc_column_inner width=&#8221;1\/2&#8243;][vc_video link=&#8221;https:\/\/youtu.be\/cnXDTjCXGjE&#8221;][\/vc_column_inner][vc_column_inner width=&#8221;1\/2&#8243;][vc_column_text]Visualizing the successful Isothermal Amplification using a simple color change! How does it work?[\/vc_column_text]<a class=\"btn btn-standard form-btn\"  href=\"https:\/\/youtu.be\/cnXDTjCXGjE\" style=\"margin-right:auto;\">Watch this video<\/a>[\/vc_column_inner][\/vc_row_inner][vc_separator color=&#8221;black&#8221;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;.vc_custom_1554393446914{margin-bottom: 30px !important;padding-top: 20px !important;padding-bottom: 20px !important;padding-left: 20px !important;background-color: #f4f1e7 !important;}&#8221;]<\/p>\n<p style=\"text-align: center;\"><strong><span style=\"font-size: 1.3em;\">For more information on LAMP, please visit also <a href=\"https:\/\/www.neb.com\/en\/applications\/dna-amplification-pcr-and-qpcr\/isothermal-amplification#tabselect0\" target=\"_blank\" rel=\"noopener noreferrer\">www.neb.com<\/a><\/span><\/strong><\/p>\n<p>[\/vc_column_text][vc_separator color=&#8221;black&#8221;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<\/p>\n<h2><span style=\"color: #f58025;\">With NEBs convenient WarmStart LAMP Kits, your LAMP experiments will be easy and reliable:<\/span><\/h2>\n<p>NEB offers a range of different tools for your LAMP\/RT-LAMP toolbox with ~20 min protocols that serve as a fast and reliable alternative to classical PCR in any molecular biology lab or even in field trials or DNA testing outside a lab.<\/p>\n<p>As a \u201cuniversal\u201d solution, we recommend using the WarmStart Multi-Purpose LAMP\/RT-LAMP 2X Master Mix (with UDG). This Master Mix uses the WarmStart RTx and the Bst 2.0 WarmStart DNA Polymerase which are unbeatable in their performance. The Mix is compatible with many detection methods, including color change, fluorescence, turbidity measurement or agarose gel. The detection of the amplification is pH independent. Thus, this master mix can also be used for DNA or RNA samples in strongly buffered or acidic solution.[\/vc_column_text][vc_column_text]This \u201cuniversal\u201d master mix is also available in a useful kit version, the WarmStart Fluorescent LAMP\/RT-LAMP Kit (with UDG), which includes a separate vial of 50x LAMP Fluorescent Dye.[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_single_image image=&#8221;39366&#8243; img_size=&#8221;full&#8221; css=&#8221;.vc_custom_1629890565342{margin-bottom: 0px !important;border-bottom-width: 0px !important;padding-bottom: 10px !important;}&#8221;][vc_column_text css=&#8221;.vc_custom_1629891267145{margin-top: 0px !important;margin-bottom: 50px !important;border-top-width: 0px !important;padding-top: 0px !important;}&#8221;]<span class=\"bildunterschrift\">The WarmStart Multi-Purpose LAMP\/RT-LAMP 2X Master Mix (with UDG) is compatible with different detection methods including color change, fluorescence, turbidity measurement or agarose gel.<\/span>[\/vc_column_text]<div class=\"vbox\">\n\t\t\t\t<span class=\"vhead\">Advantages:<\/span>\n\t\t\t\t\t<ul class=\"vlist\">\n\t\t\t\t\t\t<li><span>Reliable amplification of DNA or RNA templates in only 5-50 min!<br \/><\/span><\/li><li><span>Easy, isothermal application, no PCR cycler required<br \/><\/span><\/li><li><span>Compatible with different detection methods, such as turbidity or fluorescence or by color change \u2013 for detection by eye!<\/span><\/li>\n\t\t\t\t\t<\/ul>\n\t\t\t<\/div>[\/vc_column][\/vc_row][vc_row][vc_column][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<\/p>\n<h2><span style=\"color: #f58025;\">Using the WarmStart Colorimetric LAMP 2x Master Mixes, DNA amplification can be detected by eye \u2013 thanks to a simple, visual pH-dependent color change.<\/span><\/h2>\n<p>In our colorimetric LAMP\/RT-LAMP kits, the pH of the reaction changes during amplification in the course of dTNP incorporation to a slight acidic value. Thus, a successful amplification can be detected as a visible color change that is particularly easy to read out.[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_single_image image=&#8221;39368&#8243; img_size=&#8221;full&#8221; css=&#8221;.vc_custom_1629890803931{margin-bottom: 0px !important;border-bottom-width: 0px !important;padding-bottom: 10px !important;}&#8221;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]The colorimetric kits are therefore often used outside the lab, e.g. in point-of-care coronavirus diagnostics (<span style=\"color: #f58025;\"><a style=\"color: #f58025;\" href=\"https:\/\/www.medrxiv.org\/content\/10.1101\/2020.02.26.20028373v1.full.pdf\" target=\"_blank\" rel=\"noopener\">more info<\/a><\/span>), in field studies for zika virus detection within the World Moscito Program (<span style=\"color: #f58025;\"><a style=\"color: #f58025;\" href=\"https:\/\/www.youtube.com\/watch?v=xqwj1gR9Da8&amp;list=PLQl3WvQSRR-uSguZghiCz6W7X3hb2ZVEA&amp;index=6\" target=\"_blank\" rel=\"noopener\">more info<\/a><\/span>) or even in space.[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=&#8221;black&#8221;][vc_single_image image=&#8221;39385&#8243; img_size=&#8221;full&#8221; css=&#8221;.vc_custom_1629891093371{margin-bottom: 0px !important;border-bottom-width: 0px !important;padding-bottom: 10px !important;}&#8221;][vc_column_text css=&#8221;.vc_custom_1629891186777{margin-bottom: 0px !important;border-bottom-width: 0px !important;padding-bottom: 10px !important;}&#8221;]<span style=\"color: #f58025;\"><strong>Genes in Space:<\/strong> <\/span><br \/>\nDiagnostic DNA Detection without PCR \u2013 first successful LAMP-detection in outer space.[\/vc_column_text]<a class=\"btn btn-standard form-btn\"  href=\"https:\/\/www.neb-online.de\/genes-in-space-diagnostische-dna-detektion-ohne-pcr-erste-erfolgreiche-lamp-detektion-im-weltall\/\" style=\"margin-right:auto;\">Find our more!<\/a>[\/vc_column][\/vc_row][vc_row bwd_show_when_type=&#8221;before&#8221; bwd_show_when_date=&#8221;12\/31\/2023 23:00&#8243; bwd_tbc4vd_show_weekday=&#8221;&#8221;][vc_column][vc_separator color=&#8221;black&#8221;][vc_column_text]<\/p>\n<h2>Product table<\/h2>\n<table style=\"width: 760px;\" border=\"0\" cellpadding=\"5\">\n<tbody>\n<tr style=\"background-color: #f3f3f3; font-size: 16px;\">\n<th style=\"border-bottom: solid #eb7e2d;\" align=\"left\" width=\"400\" height=\"40px\">PRODUCT<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"88\">Prod. #<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"100\">SIZE<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" colspan=\"3\">PRICE<\/th>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">WarmStart Multi-Purpose LAMP\/RT-LAMP 2X Master Mix with UDG<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M1708S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>282 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/M1708\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb.com\/en\/M1708\" target=\"blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"NEB Info_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">WarmStart Multi-Purpose LAMP\/RT-LAMP 2X Master Mix with UDG<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M1708L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">500 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>1.129 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/M1708\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb.com\/en\/M1708\" target=\"blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"NEB Info_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">WarmStart Fluorescent LAMP\/RT-LAMP Kit with UDG<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E1708S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>290 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/E1708\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb.com\/en\/E1708\" target=\"blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"NEB Info_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">WarmStart Fluorescent LAMP\/RT-LAMP Kit with UDG<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E1708L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">500 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>1.160 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/E1708\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb.com\/en\/E1708\" target=\"blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"NEB Info_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">WarmStart Colorimetric LAMP 2X Master Mix with UDG<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M1804S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>296 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/M1804\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb.com\/en\/M1804\" target=\"blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"NEB Info_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">WarmStart Colorimetric LAMP 2X Master Mix with UDG<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M1804L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">500 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>1.182 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/M1804\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb.com\/en\/M1804\" target=\"blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"NEB Info_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E2019S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">96 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>745 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/E2019\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb.com\/en\/E2019\" target=\"blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"NEB Info_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">WarmStart<sup>\u00ae<\/sup> LAMP Kit (DNA &amp; RNA)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E1700S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>235 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/E1700\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/e1700\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">WarmStart<sup>\u00ae<\/sup> LAMP Kit (DNA &amp; RNA)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E1700L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">500 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>940 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/E1700\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/e1700\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">WarmStart<sup>\u00ae<\/sup> Colorimetric LAMP 2X Master Mix (DNA &amp; RNA)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M1800S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>232 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/M1800\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/M1800\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">WarmStart<sup>\u00ae<\/sup> Colorimetric LAMP 2X Master Mix (DNA &amp; RNA)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M1800L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">500 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>930 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/M1800\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/M1800\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p style=\"text-align: right;\"><span class=\"bildunterschrift\">As of: 01.01.2023<\/span><\/p>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row bwd_show_when_type=&#8221;after&#8221; bwd_show_when_date=&#8221;12\/31\/2023 23:00&#8243; bwd_tbc4vd_show_weekday=&#8221;&#8221;][vc_column][vc_separator color=&#8221;black&#8221;][vc_column_text]<\/p>\n<h2>Product table<\/h2>\n<table style=\"width: 760px;\" border=\"0\" cellpadding=\"5\">\n<tbody>\n<tr style=\"background-color: #f3f3f3; font-size: 16px;\">\n<th style=\"border-bottom: solid #eb7e2d;\" align=\"left\" width=\"400\" height=\"40px\">PRODUCT<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"88\">Prod. #<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"100\">SIZE<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" colspan=\"2\">INFO<\/th>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">WarmStart Multi-Purpose LAMP\/RT-LAMP 2X Master Mix with UDG<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M1708S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/M1708\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb.com\/en\/M1708\" target=\"blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"NEB Info_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">WarmStart Multi-Purpose LAMP\/RT-LAMP 2X Master Mix with UDG<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M1708L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">500 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/M1708\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb.com\/en\/M1708\" target=\"blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"NEB Info_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">WarmStart Fluorescent LAMP\/RT-LAMP Kit with UDG<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E1708S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/E1708\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb.com\/en\/E1708\" target=\"blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"NEB Info_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">WarmStart Fluorescent LAMP\/RT-LAMP Kit with UDG<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E1708L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">500 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/E1708\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb.com\/en\/E1708\" target=\"blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"NEB Info_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">WarmStart Colorimetric LAMP 2X Master Mix with UDG<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M1804S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/M1804\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb.com\/en\/M1804\" target=\"blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"NEB Info_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">WarmStart Colorimetric LAMP 2X Master Mix with UDG<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M1804L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">500 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/M1804\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb.com\/en\/M1804\" target=\"blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"NEB Info_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E2019S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">96 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/E2019\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb.com\/en\/E2019\" target=\"blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"NEB Info_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">WarmStart<sup>\u00ae<\/sup> LAMP Kit (DNA &amp; RNA)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E1700S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/E1700\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/e1700\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">WarmStart<sup>\u00ae<\/sup> LAMP Kit (DNA &amp; RNA)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E1700L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">500 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/E1700\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/e1700\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">WarmStart<sup>\u00ae<\/sup> Colorimetric LAMP 2X Master Mix (DNA &amp; RNA)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M1800S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/M1800\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/M1800\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">WarmStart<sup>\u00ae<\/sup> Colorimetric LAMP 2X Master Mix (DNA &amp; RNA)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">M1800L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">500 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/M1800\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/M1800\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p style=\"text-align: right;\"><span class=\"bildunterschrift\">As of: 01.01.2024<\/span><\/p>\n<p>[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row][vc_column][vc_basic_grid post_type=&#8221;post&#8221; max_items=&#8221;10&#8243; element_width=&#8221;12&#8243; gap=&#8221;15&#8243; item=&#8221;4123&#8243; taxonomies=&#8221;70&#8243; grid_id=&#8221;vc_gid:1702996944067-a743b33f-0a68-5&#8243;][\/vc_column][\/vc_row][vc_row css=&#8221;.vc_custom_1448619604073{margin-right: 5px !important;}&#8221;][vc_column][vc_row_inner][vc_column_inner width=&#8221;2\/3&#8243;][vc_column_text]Isothermal Amplification is becoming more and more popular as a simple and cost-efficient alternative to standard PCR.[\/vc_column_text][vc_column_text] How does LAMP (Loop-mediated isothermal amplification) work? Loop-mediated isothermal amplification (LAMP) uses 4-6 primers to amplify a specific DNA fragment at a constant temperature. A strand-displacing DNA&#8230;  <a class=\"excerpt-read-more\" href=\"https:\/\/www.neb-online.de\/en\/pcr-and-dna-amplification\/isothermal-amplification\/\" title=\"Read Isothermal Amplification\">Read more &raquo;<\/a><\/p>\n","protected":false},"author":2,"featured_media":39392,"parent":2092,"menu_order":0,"comment_status":"open","ping_status":"closed","template":"page-sidebar.php","meta":{"footnotes":""},"class_list":["post-2141","page","type-page","status-publish","has-post-thumbnail","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.1.1 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Isothermal Amplification - New England Biolabs GmbH<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.neb-online.de\/en\/pcr-and-dna-amplification\/isothermal-amplification\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Isothermal Amplification - New England Biolabs GmbH\" \/>\n<meta property=\"og:description\" content=\"[vc_row][vc_column][vc_basic_grid post_type=&#8221;post&#8221; max_items=&#8221;10&#8243; element_width=&#8221;12&#8243; gap=&#8221;15&#8243; item=&#8221;4123&#8243; taxonomies=&#8221;70&#8243; grid_id=&#8221;vc_gid:1702996944067-a743b33f-0a68-5&#8243;][\/vc_column][\/vc_row][vc_row css=&#8221;.vc_custom_1448619604073{margin-right: 5px !important;}&#8221;][vc_column][vc_row_inner][vc_column_inner width=&#8221;2\/3&#8243;][vc_column_text]Isothermal Amplification is becoming more and more popular as a simple and cost-efficient alternative to standard PCR.[\/vc_column_text][vc_column_text] How does LAMP (Loop-mediated isothermal amplification) work? Loop-mediated isothermal amplification (LAMP) uses 4-6 primers to amplify a specific DNA fragment at a constant temperature. A strand-displacing DNA... 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