{"id":21548,"date":"2020-03-04T16:15:34","date_gmt":"2020-03-04T15:15:34","guid":{"rendered":"https:\/\/www.neb-online.de\/?page_id=21548"},"modified":"2023-12-19T14:23:00","modified_gmt":"2023-12-19T13:23:00","slug":"impact-proteinexpression-und-aufreinigung","status":"publish","type":"page","link":"https:\/\/www.neb-online.de\/en\/proteinexpression\/impact-proteinexpression-und-aufreinigung\/","title":{"rendered":"IMPACT – Proteinexpression and Purification"},"content":{"rendered":"

[vc_row][vc_column][vc_basic_grid post_type=”post” max_items=”10″ element_width=”12″ gap=”15″ item=”4123″ taxonomies=”68″ grid_id=”vc_gid:1702992136656-32de403e-145f-7″][\/vc_column][\/vc_row][vc_row][vc_column width=”1\/2″][vc_column_text]IMPACT (Intein Mediated Purification with an Affinity Chitin-binding Tag) is a novel protein purification system which utilizes the inducible self-cleavage activity of a protein splicing element (termed intein) to separate the target protein from the affinity tag. It distinguishes itself from all other purification systems by its ability to purify, in a single chromatographic step, a native recombinant protein without the use of a protease. A target protein is fused to a tag consisting of the intein and the chitin binding domain which allows affinity purification of the fusion protein on a chitin column.<\/p>\n

In the presence of thiols such as DTT or 2-mercaptoethanesulfonic acid (MESNA), the intein undergoes specific self-cleavage which releases the target protein from the chitin-bound intein tag resulting in a single-column purification of the target protein.[\/vc_column_text][\/vc_column][vc_column width=”1\/2″][vc_single_image image=”21541″ img_size=”full”][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]The IMPACT Kit contains expression vectors which allow fusion of the cleavable intein tag to either the C-terminus or N-terminus of the target protein. This flexibility in fusion protein construction maximizes the probability of successful expression and purification of a target protein.[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=”custom” accent_color=”#eb7e2d”][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n

Available IMPACT products:<\/h2>\n

[\/vc_column_text][\/vc_column][\/vc_row][vc_row bwd_show_when_type=”before” bwd_show_when_date=”12\/31\/2023 23:00″ bwd_tbc4vd_show_weekday=””][vc_column][vc_column_text]<\/p>\n\n\n\n\n\n\n\n\n\n\n\n
PRODUCT<\/th>\nCatalog #<\/th>\nSIZE<\/th>\nPRICE<\/th>\n<\/tr>\n
IMPACT Kit<\/td>\nE6901S<\/td>\n1 Kit<\/td>\n448 \u20ac<\/strong><\/td>\n\"NEB<\/a><\/td>\n\"NEB<\/a><\/td>\n<\/tr>\n
Chitin Resin<\/td>\nS6651S<\/td>\n20 ml<\/td>\n106 \u20ac<\/strong><\/td>\n\"NEB<\/a><\/td>\n\"NEB<\/a><\/td>\n<\/tr>\n
Chitin Resin<\/td>\nS6651L<\/td>\n100 ml<\/td>\n424 \u20ac<\/strong><\/td>\n\"NEB<\/a><\/td>\n\"NEB<\/a><\/td>\n<\/tr>\n
Chitin Magnetic Beads<\/td>\nE8036S<\/td>\n5 ml<\/td>\n141 \u20ac<\/strong><\/td>\n\"NEB<\/a><\/td>\n\"NEB<\/a><\/td>\n<\/tr>\n
pTWIN1 Vector DNA<\/td>\nN6951S<\/td>\n10 \u00b5g<\/td>\n130 \u20ac<\/strong><\/td>\n\"NEB<\/a><\/td>\n\"NEB<\/a><\/td>\n<\/tr>\n
pTXB1 Vector DNA<\/td>\nN6707S<\/td>\n10 \u00b5g<\/td>\n136 \u20ac<\/strong><\/td>\n\"NEB<\/a><\/td>\n\"NEB<\/a><\/td>\n<\/tr>\n
pTYB21 Vector<\/td>\nN6709S<\/td>\n10 \u00b5g<\/td>\n136 \u20ac<\/strong><\/td>\n\"NEB<\/a><\/td>\n\"NEB<\/a><\/td>\n<\/tr>\n
Anti-CBD Monoclonal Antibody<\/td>\nE8034S<\/td>\n0,05 ml<\/td>\n80 \u20ac<\/strong><\/td>\n\"NEB<\/a><\/td>\n\"NEB<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

As of 03.01.2023<\/span><\/em><\/p>\n

[\/vc_column_text][\/vc_column][\/vc_row][vc_row bwd_show_when_type=”after” bwd_show_when_date=”12\/31\/2023 23:00″ bwd_tbc4vd_show_weekday=””][vc_column][vc_column_text]<\/p>\n\n\n\n\n\n\n\n\n\n\n\n
PRODUCT<\/th>\nCatalog #<\/th>\nSIZE<\/th>\nINFO<\/th>\n<\/tr>\n
IMPACT Kit<\/td>\nE6901S<\/td>\n1 Kit<\/td>\n\"NEB<\/a><\/td>\n\"NEB<\/a><\/td>\n<\/tr>\n
Chitin Resin<\/td>\nS6651S<\/td>\n20 ml<\/td>\n\"NEB<\/a><\/td>\n\"NEB<\/a><\/td>\n<\/tr>\n
Chitin Resin<\/td>\nS6651L<\/td>\n100 ml<\/td>\n\"NEB<\/a><\/td>\n\"NEB<\/a><\/td>\n<\/tr>\n
Chitin Magnetic Beads<\/td>\nE8036S<\/td>\n5 ml<\/td>\n\"NEB<\/a><\/td>\n\"NEB<\/a><\/td>\n<\/tr>\n
pTWIN1 Vector DNA<\/td>\nN6951S<\/td>\n10 \u00b5g<\/td>\n\"NEB<\/a><\/td>\n\"NEB<\/a><\/td>\n<\/tr>\n
pTXB1 Vector DNA<\/td>\nN6707S<\/td>\n10 \u00b5g<\/td>\n\"NEB<\/a><\/td>\n\"NEB<\/a><\/td>\n<\/tr>\n
pTYB21 Vector<\/td>\nN6709S<\/td>\n10 \u00b5g<\/td>\n\"NEB<\/a><\/td>\n\"NEB<\/a><\/td>\n<\/tr>\n
Anti-CBD Monoclonal Antibody<\/td>\nE8034S<\/td>\n0,05 ml<\/td>\n\"NEB<\/a><\/td>\n\"NEB<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

As of 03.01.2024<\/span><\/em><\/p>\n

[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"

[vc_row][vc_column][vc_basic_grid post_type=”post” max_items=”10″ element_width=”12″ gap=”15″ item=”4123″ taxonomies=”68″ grid_id=”vc_gid:1702992136656-32de403e-145f-7″][\/vc_column][\/vc_row][vc_row][vc_column width=”1\/2″][vc_column_text]IMPACT (Intein Mediated Purification with an Affinity Chitin-binding Tag) is a novel protein purification system which utilizes the inducible self-cleavage activity of a protein splicing element (termed intein) to separate the target protein from the affinity tag. It distinguishes itself from all other purification systems by its… Read more »<\/a><\/p>\n","protected":false},"author":2,"featured_media":42170,"parent":2254,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-sidebar.php","meta":{"footnotes":""},"class_list":["post-21548","page","type-page","status-publish","has-post-thumbnail","hentry"],"yoast_head":"\nIMPACT - Proteinexpression and Purification - New England Biolabs GmbH<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.neb-online.de\/en\/proteinexpression\/impact-proteinexpression-und-aufreinigung\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"IMPACT - Proteinexpression and Purification - New England Biolabs GmbH\" \/>\n<meta property=\"og:description\" content=\"[vc_row][vc_column][vc_basic_grid post_type=”post” max_items=”10″ element_width=”12″ gap=”15″ item=”4123″ taxonomies=”68″ grid_id=”vc_gid:1702992136656-32de403e-145f-7″][\/vc_column][\/vc_row][vc_row][vc_column width=”1\/2″][vc_column_text]IMPACT (Intein Mediated Purification with an Affinity Chitin-binding Tag) is a novel protein purification system which utilizes the inducible self-cleavage activity of a protein splicing element (termed intein) to separate the target protein from the affinity tag. 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