{"id":2175,"date":"2015-04-28T07:56:00","date_gmt":"2015-04-28T07:56:00","guid":{"rendered":"http:\/\/www.neb-online.de\/?page_id=2175"},"modified":"2023-01-05T16:22:52","modified_gmt":"2023-01-05T15:22:52","slug":"nebnext-rrna-depletion-kit","status":"publish","type":"page","link":"https:\/\/www.neb-online.de\/en\/next-generation-sequencing\/nebnext-rrna-depletion-kit\/","title":{"rendered":"RNA Depletion \u2013 Get more relevant reads from your input material"},"content":{"rendered":"

[vc_row bwd_tbc4vd_show_weekday=”” css=”.vc_custom_1595335302627{margin-bottom: 20px !important;}”][vc_column][vc_basic_grid post_type=”post” max_items=”10″ element_width=”12″ gap=”15″ item=”4123″ taxonomies=”128″ grid_id=”vc_gid:1672932114378-c3398afc-0d8b-2″][\/vc_column][\/vc_row][vc_row][vc_column width=”1\/2″][vc_column_text]<\/p>\n

Reliably remove unwanted RNA species from your NGS samples<\/strong><\/h2>\n

[\/vc_column_text][vc_column_text]In typical RNA isolations the great majority of RNA comprises rRNA, while other RNAs like mRNAs are less abundant. Abundant RNAs can conceal the biological significance of less-abundant transcripts, making their efficient and specific removal desirable. NEBNext RNA Depletion kits facilitate the removal of abundant RNAs, while ensuring retention of RNAs of interest. These kits employ the efficient RNase H method: Single-stranded DNA probes hybridize specifically to rRNA molecules and subsequently RNase H degrades the hybridized RNA.<\/p>\n

The special feature of the NEBNext kits: <\/strong><\/span>The DNA probes cover the entire rRNA sequence so that the probes can also hybridize to fragmented rRNA.
\nTherefore, our Next-Gen-Seq validated NEBNext rRNA Depeletion Kits can successfully and reliably be used even on poor quality total RNA samples (such as fragmented RNAs or RNA from FFPE material) starting from RIN >1!<\/p>\n

Both non-coding regulatory RNA and fragmented mRNA are retained in the sample, enabling an unbiased glimpse of the transcriptome.<\/p>\n

With the NEBNext rRNA Depletion Kits, you typically need standard input amounts of 10 ng to 1 \u00b5g of total RNA. The protocol takes only 2 hours of time with less than 10 min hands-on time.[\/vc_column_text][\/vc_column][vc_column width=”1\/2″][vc_column_text css=”.vc_custom_1651741406701{margin-bottom: 4px !important;}”]NEBNext rRNA Depletion Kit Workflow
\n<\/strong>Please click to enlarge[\/vc_column_text][vc_single_image image=”1235″ img_size=”full” onclick=”link_image”][vc_separator]

\n\t\t\t\tAdvantages:<\/span>\n\t\t\t\t\t