{"id":21770,"date":"2020-03-10T11:30:16","date_gmt":"2020-03-10T10:30:16","guid":{"rendered":"https:\/\/www.neb-online.de\/?page_id=21770"},"modified":"2023-12-19T14:24:30","modified_gmt":"2023-12-19T13:24:30","slug":"k-lactis-protein-expression-kit","status":"publish","type":"page","link":"https:\/\/www.neb-online.de\/en\/proteinexpression\/k-lactis-protein-expression-kit\/","title":{"rendered":"K. lactis Protein Expression Kit"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column][vc_basic_grid post_type=&#8221;post&#8221; max_items=&#8221;10&#8243; element_width=&#8221;12&#8243; gap=&#8221;15&#8243; item=&#8221;4123&#8243; taxonomies=&#8221;68&#8243; grid_id=&#8221;vc_gid:1702992236417-2416c03e-94af-4&#8243;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]The K. lactis Expression Kit provides an easy method for expressing a gene of interest in the yeast\u00a0<em>Kluyveromyces lactis<\/em>.<\/p>\n<p>Proteins may be produced intracellularly or be secreted using the supplied integrative expression vector pKLAC2. To achieve protein secretion, a gene of interest is cloned downstream of the <em>K. lactis<\/em>\u00a0\u03b1-mating factor secretion domain (\u03b1-MF) which is eventually processed in the Golgi resulting in secretion of the desired protein.[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243;]<div class=\"vbox\">\n\t\t\t\t<span class=\"vhead\">Advantages:<\/span>\n\t\t\t\t\t<ul class=\"vlist\">\n\t\t\t\t\t\t<li><span>Supplied with competent K. lactis cells (NEB# C1001)<br \/><\/span><\/li><li><span>Allows cloning and expression of genes toxic to E. coli<br \/><\/span><\/li><li><span>Rapid high cell density growth results in high yield protein expression<br \/><\/span><\/li><li><span>Easy-to-use protocols for those inexperienced with yeast systems<br \/><\/span><\/li><li><span>No expensive antibiotics or methanol required<br \/><\/span><\/li><li><span>Attractive commercial sub-licensing<\/span><\/li>\n\t\t\t\t\t<\/ul>\n\t\t\t<\/div>[\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n<h3>The <em>K. lactis<\/em> expression system offers several advantages over other yeast and bacterial protein expression systems:<\/h3>\n<p>First,<em> K. lactis<\/em> has been used to produce proteins at industrial scale in the food industry for over a decade due to its ability to rapidly achieve high culture densities and abundantly produce recombinant proteins.<\/p>\n<p>Second, yeast expression is driven by a variant of the strong LAC4 promoter that has been modified to lack background expression in <em>E. coli<\/em>. Therefore, genes toxic to <em>E. coli<\/em> can be cloned into pKLAC2 in bacteria prior to their expression in yeast.<\/p>\n<p>Third, the kit includes highly competent <em>K. lactis<\/em> cells making the technology easy-to-use for those not accustomed to working with yeast. Their high transformation efficiency makes the system suitable for methods that require large numbers of transformants, for example, expression cloning using cDNA libraries. Selection of yeast transformants uses a unique antibiotic-free method in which acetamidase (amdS) expressed from pKLAC2 permits transformed cells to utilize acetamide as a sole nitrogen source on defined medium. Acetamide selection promotes formation of cells containing multiple integrations of pKLAC2 which results in higher yields of protein.<\/p>\n<p>Finally, proteins expressed in <em>K. lactis<\/em> have access to eukaryotic protein folding and glycosylation machinery that <em>E. coli<\/em> cells do not possess, making it an important alternative to bacterial expression systems.[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]In the nucleus, an integrated expression vector encoding a fusion between the \u03b1-MF domain (blue) and a desired protein (black) is expressed. A signal peptide in the \u03b1-MF domain directs entry of the fusion protein into the endoplasmic reticulum (ER) and is removed by signal peptidase (SP). The fusion protein is transported to the Golgi where the Kex protease removes the \u03b1-MF domain. The protein of interest is then secreted from the cell.[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_single_image image=&#8221;21735&#8243; img_size=&#8221;full&#8221;][\/vc_column][\/vc_row][vc_row bwd_show_when_type=&#8221;before&#8221; bwd_show_when_date=&#8221;12\/31\/2023 23:00&#8243; bwd_tbc4vd_show_weekday=&#8221;&#8221;][vc_column][vc_column_text]<\/p>\n<h2>Available <em>K. lactis<\/em> products:<\/h2>\n<p>[\/vc_column_text][vc_column_text]<\/p>\n<table style=\"width: 760px;\" border=\"0\" cellpadding=\"5\">\n<tbody>\n<tr style=\"background-color: #f3f3f3;\">\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"400\" height=\"40px\">PRODUCT<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"88\">Cat.-#:<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"100\">SIZE<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" colspan=\"3\">PRICE<\/th>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">K. lactis Protein Expression Kit<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E1000S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">1 Kit<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>965 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/E1000\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb.com\/en\/E1000\" target=\"blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"NEB Info_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">K. lactis GG799 Competent Cells<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">C1001S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">5 rxn<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>404 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/C1001\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb.com\/en\/C1001\" target=\"blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"NEB Info_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Yeast Carbon Base Medium Pack ( K. lactis)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">B9017S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">12,0 g<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>48 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/B9017\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb.com\/en\/B9017\" target=\"blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"NEB Info_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p style=\"text-align: right;\"><em><span class=\"bildunterschrift\">As of: 03.01.2023<\/span><\/em><\/p>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row bwd_show_when_type=&#8221;after&#8221; bwd_show_when_date=&#8221;12\/31\/2023 23:00&#8243; bwd_tbc4vd_show_weekday=&#8221;&#8221;][vc_column][vc_column_text]<\/p>\n<h2>Available <em>K. lactis<\/em> products:<\/h2>\n<p>[\/vc_column_text][vc_column_text]<\/p>\n<table style=\"width: 760px;\" border=\"0\" cellpadding=\"5\">\n<tbody>\n<tr style=\"background-color: #f3f3f3;\">\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"400\" height=\"40px\">PRODUCT<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"88\">Cat.-#:<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"100\">SIZE<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" colspan=\"2\">INFO<\/th>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">K. lactis Protein Expression Kit<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E1000S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">1 Kit<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/E1000\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb.com\/en\/E1000\" target=\"blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"NEB Info_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">K. lactis GG799 Competent Cells<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">C1001S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">5 rxn<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/C1001\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb.com\/en\/C1001\" target=\"blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"NEB Info_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">Yeast Carbon Base Medium Pack ( K. lactis)<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">B9017S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">12,0 g<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/B9017\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb.com\/en\/B9017\" target=\"blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"NEB Info_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p style=\"text-align: right;\"><em><span class=\"bildunterschrift\">As of: 03.01.2024<\/span><\/em><\/p>\n<p>[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row][vc_column][vc_basic_grid post_type=&#8221;post&#8221; max_items=&#8221;10&#8243; element_width=&#8221;12&#8243; gap=&#8221;15&#8243; item=&#8221;4123&#8243; taxonomies=&#8221;68&#8243; grid_id=&#8221;vc_gid:1702992236417-2416c03e-94af-4&#8243;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]The K. lactis Expression Kit provides an easy method for expressing a gene of interest in the yeast\u00a0Kluyveromyces lactis. Proteins may be produced intracellularly or be secreted using the supplied integrative expression vector pKLAC2. To achieve protein secretion, a gene of interest is cloned downstream of the&#8230;  <a class=\"excerpt-read-more\" href=\"https:\/\/www.neb-online.de\/en\/proteinexpression\/k-lactis-protein-expression-kit\/\" title=\"Read K. lactis Protein Expression Kit\">Read more &raquo;<\/a><\/p>\n","protected":false},"author":2,"featured_media":42170,"parent":2254,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-sidebar.php","meta":{"footnotes":""},"class_list":["post-21770","page","type-page","status-publish","has-post-thumbnail","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.1.1 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>K. lactis Protein Expression Kit - New England Biolabs GmbH<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.neb-online.de\/en\/proteinexpression\/k-lactis-protein-expression-kit\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"K. lactis Protein Expression Kit - New England Biolabs GmbH\" \/>\n<meta property=\"og:description\" content=\"[vc_row][vc_column][vc_basic_grid post_type=&#8221;post&#8221; max_items=&#8221;10&#8243; element_width=&#8221;12&#8243; gap=&#8221;15&#8243; item=&#8221;4123&#8243; taxonomies=&#8221;68&#8243; grid_id=&#8221;vc_gid:1702992236417-2416c03e-94af-4&#8243;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]The K. lactis Expression Kit provides an easy method for expressing a gene of interest in the yeast\u00a0Kluyveromyces lactis. Proteins may be produced intracellularly or be secreted using the supplied integrative expression vector pKLAC2. To achieve protein secretion, a gene of interest is cloned downstream of the... Read more &raquo;\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.neb-online.de\/en\/proteinexpression\/k-lactis-protein-expression-kit\/\" \/>\n<meta property=\"og:site_name\" content=\"New England Biolabs GmbH\" \/>\n<meta property=\"article:modified_time\" content=\"2023-12-19T13:24:30+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2020\/01\/Homebanner_Expression_Express.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1080\" \/>\n\t<meta property=\"og:image:height\" content=\"284\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"6 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.neb-online.de\/en\/proteinexpression\/k-lactis-protein-expression-kit\/\",\"url\":\"https:\/\/www.neb-online.de\/en\/proteinexpression\/k-lactis-protein-expression-kit\/\",\"name\":\"K. lactis Protein Expression Kit - New England Biolabs GmbH\",\"isPartOf\":{\"@id\":\"https:\/\/www.neb-online.de\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/www.neb-online.de\/en\/proteinexpression\/k-lactis-protein-expression-kit\/#primaryimage\"},\"image\":{\"@id\":\"https:\/\/www.neb-online.de\/en\/proteinexpression\/k-lactis-protein-expression-kit\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2020\/01\/Homebanner_Expression_Express.jpg\",\"datePublished\":\"2020-03-10T10:30:16+00:00\",\"dateModified\":\"2023-12-19T13:24:30+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/www.neb-online.de\/en\/proteinexpression\/k-lactis-protein-expression-kit\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/www.neb-online.de\/en\/proteinexpression\/k-lactis-protein-expression-kit\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/www.neb-online.de\/en\/proteinexpression\/k-lactis-protein-expression-kit\/#primaryimage\",\"url\":\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2020\/01\/Homebanner_Expression_Express.jpg\",\"contentUrl\":\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2020\/01\/Homebanner_Expression_Express.jpg\",\"width\":1080,\"height\":284},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/www.neb-online.de\/en\/proteinexpression\/k-lactis-protein-expression-kit\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/www.neb-online.de\/en\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Protein Expression\",\"item\":\"https:\/\/www.neb-online.de\/en\/proteinexpression\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"K. lactis Protein Expression Kit\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/www.neb-online.de\/#website\",\"url\":\"https:\/\/www.neb-online.de\/\",\"name\":\"New England Biolabs GmbH\",\"description\":\"Enabling Technologies in the Life Sciences\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/www.neb-online.de\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"K. lactis Protein Expression Kit - New England Biolabs GmbH","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.neb-online.de\/en\/proteinexpression\/k-lactis-protein-expression-kit\/","og_locale":"en_US","og_type":"article","og_title":"K. lactis Protein Expression Kit - New England Biolabs GmbH","og_description":"[vc_row][vc_column][vc_basic_grid post_type=&#8221;post&#8221; max_items=&#8221;10&#8243; element_width=&#8221;12&#8243; gap=&#8221;15&#8243; item=&#8221;4123&#8243; taxonomies=&#8221;68&#8243; grid_id=&#8221;vc_gid:1702992236417-2416c03e-94af-4&#8243;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]The K. lactis Expression Kit provides an easy method for expressing a gene of interest in the yeast\u00a0Kluyveromyces lactis. Proteins may be produced intracellularly or be secreted using the supplied integrative expression vector pKLAC2. To achieve protein secretion, a gene of interest is cloned downstream of the... 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