{"id":2191,"date":"2015-04-28T08:11:26","date_gmt":"2015-04-28T08:11:26","guid":{"rendered":"http:\/\/www.neb-online.de\/?page_id=2191"},"modified":"2023-01-13T11:02:35","modified_gmt":"2023-01-13T10:02:35","slug":"rna-biology","status":"publish","type":"page","link":"https:\/\/www.neb-online.de\/en\/rna-biology\/","title":{"rendered":"RNA Biology"},"content":{"rendered":"

[vc_row][vc_column][vc_basic_grid post_type=”post” max_items=”10″ element_width=”12″ gap=”15″ item=”4123″ taxonomies=”131″ grid_id=”vc_gid:1673604050077-02a41777-3df8-0″][\/vc_column][\/vc_row][vc_row][vc_column][vc_row_inner][vc_column_inner width=”2\/3″][vc_column_text]Historically the function of RNA in the cell was to assist in the translation of genetic information from DNA into protein. The major species of RNA were; (A) messenger RNA, which converts the genetic information of DNA into RNA, (B) transfer RNA (tRNA) that are charged with specific amino acids and, (C) ribosomal RNA (rRNA), a major component of the ribosome. \u00a0RNA has now been implicated in a diverse number of biological processes including catalysis and transcriptional regulation. Recently, technological advances and improvements in RNA analysis and detection have led to the discovery of many new classes of small and large non-coding RNAs with novel regulatory functions.\u00a0 Examples include, microRNA (miRNA), circular RNA, long\u00a0 non-coding RNA (lncRNA), small nucleolar RNA (snoRNA) and extracullular RNA (exRNA). In addition, RNA modifications have revealed added complexity to RNA. These biologically relevant modifications are an active area of exploration. These findings have helped usher in a renaissance of RNA-focused research in biology.[\/vc_column_text][\/vc_column_inner][vc_column_inner width=”1\/3″][vc_single_image image=”14635″ img_size=”full” onclick=”link_image”][\/vc_column_inner][\/vc_row_inner][vc_column_text]Key capabilities for contemporary RNA research include RNA synthesis<\/a> for\u00a0in vitro<\/em>\u00a0analysis or transfection, RNA modification<\/a> and processing<\/a>, reverse transcription <\/a>of RNA for cloning and expression analysis, and detection of RNA<\/a>.<\/p>\n

NEBs\u00a0Reverse Transcriptases can be found\u00a0here<\/a>.[\/vc_column_text][\/vc_column][\/vc_row][vc_row css=”.vc_custom_1520344098843{margin-right: -1px !important;margin-left: -1px !important;background-color: #f3f3f3 !important;}”][vc_column][vc_column_text]<\/p>\n

Videos<\/h2>\n

[\/vc_column_text][vc_row_inner][vc_column_inner width=”1\/2″][vc_video link=”https:\/\/www.youtube.com\/watch?v=OoBITqzcy1Y”][vc_column_text]NEBNext Ultra II Directional RNA Workflow<\/p>\n

Learn more about the streamlined workflow for the NEBNext Ultra II Directional RNA Library Prep Kit.[\/vc_column_text][\/vc_column_inner][vc_column_inner width=”1\/2″][vc_video link=”https:\/\/www.youtube.com\/watch?v=jgPUOHX0k3c”][vc_column_text]<\/p>\n

In vitro<\/em> Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection<\/h3>\n

This method describes high yield in vitro<\/em> synthesis of both capped and uncapped mRNA from a linearized plasmid containing the GLuc gene.[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n

Example: mRNA Synthesis Workflow & related NEB products<\/h2>\n

(Click on the respective grey box to receive further product information)[\/vc_column_text][vc_column_text]<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
TEMPLATE GENERATION<\/span><\/td>\nIN VITRO<\/em> TRANSCRIPTION<\/span><\/td>\nRNA CAPPING<\/span><\/td>\nPOLY(A) TAILING<\/span><\/td>\nRNA PURIFICATION<\/span><\/td>\n<\/tr>\n
<\/td>\n<\/tr>\n
Q5 High Fidelity DNA Polymerase<\/span><\/a><\/td>\nHiScribe T7 mRNA Kit with CleanCap Reagent AG<\/span><\/a><\/td>\nE. coli<\/em> Poly(A) Polymerase<\/span><\/a><\/td>\nMonarch RNA Cleanup Kit (10 \u03bcg)<\/span><\/a><\/td>\n<\/tr>\n
HiScribe T7 ARCA mRNA Synthesis Kit (with tailing)<\/span><\/a><\/td>\n<\/tr>\n
dNTP solution mixes<\/span><\/a><\/td>\nHiScribe T7 ARCA mRNA Synthesis Kit<\/span><\/a><\/td>\n<\/td>\nMonarch RNA Cleanup Kit (50 \u03bcg)<\/span><\/a><\/td>\n<\/tr>\n
BspQI*<\/span><\/a><\/td>\nHiScribe T7 High Yield RNA Synthesis Components<\/span><\/a><\/td>\nFaustovirus Capping Enzyme <\/span><\/a><\/td>\n<\/td>\nMonarch RNA Cleanup Kit (500 \u03bcg)<\/span><\/a><\/td>\n<\/tr>\n
DNA Assembly<\/span><\/p>\nHiScribe T7 Quick High Yield RNA Synthesis Kit<\/span><\/a><\/td>\nVaccinia Capping System<\/span><\/a><\/td>\n<\/td>\nLithium Chloride<\/span><\/a><\/td>\n<\/tr>\n
HiScribe SP6 High Yield RNA Synthesis Kit<\/span><\/a><\/td>\nmRNA Cap 2\u00b4-O-Methyltranferase<\/span><\/a><\/td>\n<\/td>\n<\/td>\n<\/tr>\n
T3 & SP6 RNA Polymerases<\/span><\/a><\/td>\nARCA and other mRNA cap analogs<\/span><\/a><\/td>\n<\/td>\n<\/td>\n<\/tr>\n
T7 RNA Polymerase<\/span><\/a><\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
Hi-T7 RNA Polymerase<\/span><\/a><\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
Companion Products<\/span><\/strong><\/td>\n<\/td>\n<\/td>\nCompanion Products<\/span><\/strong><\/td>\n<\/tr>\n
RNase inhibitor (Murine)<\/span><\/a><\/td>\n<\/td>\n<\/td>\nMonarch RNA Cleanup Binding Buffer<\/span><\/a><\/td>\n<\/tr>\n
RNase Inhibitor (Human Placental)<\/span><\/a><\/td>\n<\/td>\n<\/td>\nMonarch RNA Cleanup Wash Buffer<\/span><\/a><\/td>\n<\/tr>\n
Pyrophosphatase, Inorganic (E. coli<\/em>)<\/span><\/a><\/td>\n<\/td>\n<\/td>\nNuclease-free Water<\/span><\/a><\/td>\n<\/tr>\n
Pyrophosphatase, Inorganic (Yeast)<\/span><\/a><\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
DNase I (RNase-free)<\/span><\/a><\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
DNase I-XT<\/span><\/a><\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
NTPs<\/span><\/a><\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

* NEB can offer large-scale preparations of restriction enzymes using Recombinant Albumin (BSA-free)<\/p>\n

[\/vc_column_text][vc_column_text]Information on Reverse Transcription \/ cDNA Synthesis from NEB can be found here<\/a>.
\nYou can also learn more about: Monarch RNA Cleanup Kits (purification\/concentration)<\/a>, Monarch Total RNA Miniprep Kit<\/a> and NEBNext Ultra II RNA Library Prep Kit and modules<\/a>![\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"

[vc_row][vc_column][vc_basic_grid post_type=”post” max_items=”10″ element_width=”12″ gap=”15″ item=”4123″ taxonomies=”131″ grid_id=”vc_gid:1673604050077-02a41777-3df8-0″][\/vc_column][\/vc_row][vc_row][vc_column][vc_row_inner][vc_column_inner width=”2\/3″][vc_column_text]Historically the function of RNA in the cell was to assist in the translation of genetic information from DNA into protein. The major species of RNA were; (A) messenger RNA, which converts the genetic information of DNA into RNA, (B) transfer RNA (tRNA) that are charged with… Read more »<\/a><\/p>\n","protected":false},"author":2,"featured_media":40787,"parent":0,"menu_order":0,"comment_status":"open","ping_status":"closed","template":"page-sidebar.php","meta":{"footnotes":""},"class_list":["post-2191","page","type-page","status-publish","has-post-thumbnail","hentry"],"yoast_head":"\nRNA Biology - New England Biolabs GmbH<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.neb-online.de\/en\/rna-biology\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"RNA Biology - New England Biolabs GmbH\" \/>\n<meta property=\"og:description\" content=\"[vc_row][vc_column][vc_basic_grid post_type=”post” max_items=”10″ element_width=”12″ gap=”15″ item=”4123″ taxonomies=”131″ grid_id=”vc_gid:1673604050077-02a41777-3df8-0″][\/vc_column][\/vc_row][vc_row][vc_column][vc_row_inner][vc_column_inner width=”2\/3″][vc_column_text]Historically the function of RNA in the cell was to assist in the translation of genetic information from DNA into protein. 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