{"id":2206,"date":"2015-04-28T08:37:50","date_gmt":"2015-04-28T08:37:50","guid":{"rendered":"http:\/\/www.neb-online.de\/?page_id=2206"},"modified":"2026-01-26T13:16:51","modified_gmt":"2026-01-26T12:16:51","slug":"competent-cells","status":"publish","type":"page","link":"https:\/\/www.neb-online.de\/en\/competent-cells\/","title":{"rendered":"Competent cells from NEB"},"content":{"rendered":"

[vc_row bwd_show_when_type=”before” bwd_show_when_date=”10\/01\/2024 02:00″ bwd_tbc4vd_show_weekday=””][vc_column][vc_column_text]<\/p>\n

Save your budget: Buy competent cells from NEB<\/span><\/strong><\/h2>\n

[\/vc_column_text][\/vc_column][\/vc_row][vc_row bwd_tbc4vd_show_weekday=”” disable_element=”yes”][vc_column][vc_basic_grid post_type=”post” max_items=”10″ element_width=”12″ gap=”15″ item=”4123″ grid_id=”vc_gid:1727346535113-4f7275b5-d8fc-5″ taxonomies=”125″][\/vc_column][\/vc_row][vc_row bwd_show_when_type=”before” bwd_show_when_date=”10\/01\/2024 02:00″ bwd_tbc4vd_show_weekday=””][vc_column width=”2\/3″][vc_column_text]Take advantage of the NEB golden jubilee summer offer and receive a 25% discount<\/strong> on the list price and an additional NEB Tube Opener<\/strong> when purchasing competent cells.* Simply enter the promo code “CompCells24<\/strong>” when ordering.<\/p>\n

*The offer is valid up to and including 30.09.2024 and cannot be combined with other discounts. As long as stocks last.<\/span><\/em>[\/vc_column_text][\/vc_column][vc_column width=”1\/3″][vc_single_image image=”47466″ img_size=”full” alignment=”center”][\/vc_column][\/vc_row][vc_row bwd_show_when_type=”before” bwd_show_when_date=”10\/01\/2024 02:00″ bwd_tbc4vd_show_weekday=””][vc_column][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n

Cloning Strains<\/strong><\/span><\/h2>\n

For cloning experiments choose from several high efficiency competent cell strains<\/a>. These\u00a0E. coli<\/em>\u00a0strains are T1 phage resistant and are\u00a0endA\u00a0<\/em>deficient for high quality plasmid preparations. Additionally, all competent cells from NEB are free of animal products.<\/p>\n

Yeast<\/strong><\/span><\/h2>\n

NEB offers chemically competent\u00a0Kluyveromyces\u00a0<\/em>lactis<\/em><\/a>\u00a0cells and variants of this strain that have been tailored for specific protein expression needs. These cells are suitable for transformation with any of our linearized pKLAC series expression vectors.<\/p>\n

E. coli<\/em>\u00a0for Protein Expression<\/strong><\/span><\/h2>\n

NEB offers several strains<\/a> with varying levels of expression control, each with T1 phage resistance and extremely high transformation efficiencies.<\/p>\n

As Starter kit<\/strong> NEB recommends the Cloning Competent E.coli<\/em> Sampler<\/a>. It contains the four cloning strains at a preferential price.[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=”custom” accent_color=”#919191″][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n

Competent\u00a0E. coli<\/em> – Comparison Table<\/span><\/strong><\/h2>\n

[\/vc_column_text][vc_column_text]Use this table to select another company’s competent cell product and find out which NEB strain is compatible. Choose either the product name or catalog number from the available selection, and this tool will identify the recommended NEB product and its advantages. A link to the product page where you can also order the product is provided.
\nCompetent Cell Product Comparison Table<\/strong><\/a>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=”custom” accent_color=”#919191″][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=”.vc_custom_1439991969375{padding-top: 0px !important;}”]<\/p>\n

Properties Table<\/h2>\n

[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Product<\/span><\/th>\nNEB #<\/span><\/th>\nCharacteristics<\/span><\/th>\n<\/tr>\n<\/thead>\n
Strains for Cloning and Subcloning<\/th>\n<\/tr>\n
NEB Stable Competent E. coli\u00a0<\/em>(High Efficiency)<\/span><\/a><\/td>\nC3040H<\/p>\n

C3040I<\/td>\n

\n
    \n
  • High quality plasmid preparations due to endA<\/em> mutation<\/li>\n
  • Rapid growth recA<\/em> strain<\/li>\n
  • T1 phage resistance (fhuA)<\/em><\/li>\n
  • Value pricing<\/li>\n
  • Free of animal products<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
NEB 5-alpha Competent E. coli<\/em> (High Efficiency)<\/span><\/a><\/td>\nC2987H<\/p>\n

C2987I<\/p>\n

C2987P<\/p>\n

C2987R<\/p>\n

C2987U<\/td>\n

\n
    \n
  • Versatile cloning strain<\/li>\n
  • DH5\u03b1\u2122 derivative<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
NEB 5-alpha F’ Iq<\/sup> Competent E. coli<\/em> (High Efficiency)<\/span><\/a><\/td>\nC2992H<\/p>\n

C2992I<\/td>\n

\n
    \n
  • Toxic gene cloning<\/li>\n
  • F\u00b4 strain with extremely high transformation efficiency<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
NEB 5-alpha Competent E. coli<\/em> (Subcloning Efficiency)<\/span><\/a><\/td>\nC2988J<\/td>\n\n
    \n
  • Ideal for subcloning efficiency transformations, such as plasmid transformation or routine subcloning<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
NEB 10-beta Competent E. coli<\/em> (High Efficiency)<\/span><\/a><\/td>\nC3019H<\/p>\n

C3019I<\/td>\n

\n
    \n
  • Large plasmid and BAC cloning<\/li>\n
  • DH10B\u2122 derivative<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
dam–<\/sup>\/dcm–<\/sup><\/em> Competent <\/span>E. coli<\/em><\/span><\/a><\/td>\nC2925H<\/p>\n

C2925I<\/td>\n

\n
    \n
  • Dam\/Dcm methyltransferase free plasmid growth<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
NEB Turbo Competent E. coli<\/em> (High Efficiency)<\/span><\/a><\/td>\nC2984H<\/p>\n

C2984I<\/td>\n

\n
    \n
  • Fastest growth \u2013 colonies visible after 6.5 hours<\/li>\n
  • Plasmid preparation after 4 hours<\/li>\n
  • Tight control\/expression of toxic genes (F\u00b4lacI8)<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
Strains for Protein Expression<\/th>\n<\/tr>\n
NEB Express Competent E. coli<\/em> (High Efficiency)<\/span><\/a><\/td>\nC2523H<\/p>\n

C2523I<\/td>\n

\n
    \n
  • Versatile non-T7 expression strain<\/li>\n
  • Protease deficient<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
NEB Express Iq<\/sup><\/em> Competent E. coli<\/em> (High Efficiency)<\/span><\/a><\/td>\nC3037I<\/td>\n\n
    \n
  • Control of IPTG induced expression from Plac<\/sub>,<\/em> Ptac<\/sub><\/em>,Ptrc <\/sub>and T5lac<\/em><\/sub><\/li>\n
  • Protease deficient<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
T7 Express Competent E. coli<\/em> (High Efficiency)<\/span><\/a><\/td>\nC2566H<\/p>\n

C2566I<\/p>\n

 <\/td>\n

\n
    \n
  • Most popular T7 expression strain<\/li>\n
  • Protease deficient<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
T7 Express lysY<\/em> Competent E. coli<\/em> (High Efficiency)<\/a><\/span><\/td>\nC3010I<\/p>\n

 <\/td>\n

\n
    \n
  • T7 expression<\/li>\n
  • Protease deficient<\/li>\n
  • Better reduction of basal expression<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
T7 Express lysY\/Iq<\/sup><\/em> Competent E. coli<\/em> (High Efficiency)<\/a><\/span><\/td>\nC3013I<\/p>\n

 <\/td>\n

\n
    \n
  • T7 expression<\/li>\n
  • Protease deficient<\/li>\n
  • Highest level of expression control<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
T7 Express Crystal Competent E. coli<\/em> (High Efficiency)<\/span><\/a><\/td>\nC3022I<\/td>\n\n
    \n
  • T7 expression<\/li>\n
  • Protease deficient<\/li>\n
  • SeMet labeling for protein crystallography<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
SHuffle Express Competent <\/span>E.\u2009coli<\/span><\/a><\/td>\nC3028J<\/td>\n\n
    \n
  • Protease deficient\/B strain<\/li>\n
  • Enhanced capacity to correctly fold proteins with multiple disulfide bonds in the cytoplasm<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
SHuffle T7 Express Competent <\/span>E. coli<\/em><\/span><\/a><\/td>\nC3029J<\/td>\n\n
    \n
  • T7 expression<\/li>\n
  • Protease deficient\/B strain<\/li>\n
  • Enhanced capacity to correctly fold proteins with multiple disulfide bonds in the cytoplasm<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
SHuffle T7 Express lysY<\/em> Competent <\/span>E. coli<\/em><\/span><\/a><\/td>\nC3030J<\/td>\n\n
    \n
  • T7 expression<\/li>\n
  • Protease deficient\/B strain<\/li>\n
  • Tightly controlled expression of toxic proteins<\/li>\n
  • Enhanced capacity to correctly fold proteins with multiple disulfide bonds in the cytoplasm<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
SHuffle T7 Competent <\/span>E. coli<\/em><\/span><\/a><\/td>\nC3026J<\/td>\n\n
    \n
  • T7 expression\/K12 strain<\/li>\n
  • Enhanced capacity to correctly fold proteins with multiple disulfide bonds in the cytoplasm<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
BL21 Competent <\/span>E. coli<\/em><\/span><\/a><\/td>\nC2530H<\/td>\n\n
    \n
  • Routine non-T7 expression<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
BL21(DE3) Competent <\/span>E. coli<\/em><\/span><\/a><\/td>\nC2527H<\/p>\n

C2527I<\/td>\n

\n
    \n
  • Routine T7 expression<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
Lemo21(DE3) Competent <\/span>E. coli<\/em><\/span><\/a><\/td>\nC2528J<\/td>\n\n
    \n
  • Tunable T7 expression for difficult targets<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
NiCo21(DE3) Competent <\/span>E. coli<\/em><\/span><\/a><\/td>\nC2529H<\/td>\n\n