{"id":2254,"date":"2015-04-29T08:02:46","date_gmt":"2015-04-29T08:02:46","guid":{"rendered":"http:\/\/www.neb-online.de\/?page_id=2254"},"modified":"2021-09-14T09:13:14","modified_gmt":"2021-09-14T07:13:14","slug":"proteinexpression","status":"publish","type":"page","link":"https:\/\/www.neb-online.de\/en\/proteinexpression\/","title":{"rendered":"Protein Expression"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column][vc_basic_grid post_type=&#8221;post&#8221; max_items=&#8221;10&#8243; element_width=&#8221;12&#8243; gap=&#8221;15&#8243; item=&#8221;4123&#8243; taxonomies=&#8221;75&#8243; grid_id=&#8221;vc_gid:1631603551263-06b345da-ccba-6&#8243;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n<h2>It\u2019s a matter of expression.<\/h2>\n<p>Protein expression can be a very complex, multi-factorial process. Each protein requires a specific intracellular environment to correctly achieve its secondary and tertiary structures. Proteins may also require post-translational modifications or insertion into a cellular membrane for proper function. Other proteins, once expressed, may be toxic to the host. Thus, no single solution exists for the successful production of all recombinant proteins, and a broad range of expression tools is needed to ensure the successful expression of your target protein.[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]Our NEBExpress portfolio of products includes solutions for expression and purification, and is supported by access to scientists with over 40 years of experience in developing and using recombinant protein technologies in E. coli. We use these solutions in our own research and manufacturing processes, and know that quality and performance are critical \u2013 all of our products are stringently tested so that you can be sure they will work optimally for your solution, just as we rely on them to work in ours.[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_text_separator title=&#8221;Video: Expression of difficult Proteins&#8221; color=&#8221;custom&#8221; accent_color=&#8221;#eb7e2d&#8221;][vc_video link=&#8221;https:\/\/youtu.be\/L5owMAtnVnQ&#8221;][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=&#8221;custom&#8221; accent_color=&#8221;#eb7e2d&#8221;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n<div id=\"main_0_wideleftcolumn_0_divContainer\" class=\"line wide pane group\">\n<article class=\"column-left\">\n<div class=\"inner\">\n<h2>Protein Expression Systems:<\/h2>\n<h3>Generate analytical amounts of protein with our cell-free expression systems<\/h3>\n<ul>\n<li>The <a href=\"https:\/\/www.neb-online.de\/en\/proteinexpression\/purexpress-in-vitro-protein-synthesis-kit\/\">PURExpress <em>In Vitro<\/em> Protein Synthesis Kit<\/a>, our cell-free transcription\/translation system, enables protein expression in approximately two hours and is ideal for high throughput technologies<\/li>\n<li><a href=\"https:\/\/www.neb-online.de\/en\/proteinexpression\/nebxpress-cell-free-e-coli-proteine-synthesis-system\/\">NEBExpress <em>Cell-free<\/em>\u00a0E. coli\u00a0Protein Synthesis System<\/a>, our novel\u00a0E. coli\u00a0cell-extract based transcription\/translation system, is designed to synthesize proteins under the control of T7 RNA Polymerase at high yields for a wide variety of proteins<\/li>\n<\/ul>\n<h3>Generate and purify high yields of recombinant proteins<\/h3>\n<ul>\n<li>The <a title=\"pMAL\" href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=E8201\">NEBExpress MBP Fusion and Purification System<\/a> utilizes a pMAL vector and the\u00a0malE\u00a0gene for the expression of MBP-fusion proteins, which can be isolated by a one-step affinity purification<\/li>\n<li>The <a title=\"IMPACT\" href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=E6901\">IMPACT Kit<\/a> utilizes engineered protein splicing elements (inteins) to purify recombinant proteins in a single step<\/li>\n<li>For analysis, try our\u00a0<a href=\"https:\/\/www.neb-online.de\/en\/dna-rna-markers-and-ladders\/protein-markers\/\">protein standards<\/a>, which are available unstained, prestained, or with two colors for easy identification<\/li>\n<\/ul>\n<h3>Optimized cells for protein expression<\/h3>\n<ul>\n<li>Discover our optimized <a href=\"https:\/\/www.neb-online.de\/en\/competent-cells\/e-coli-expression-strains\/\">protein expressions strains<\/a><\/li>\n<\/ul>\n<h3>Eukaryotic protein expression<\/h3>\n<ul>\n<li><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=E1000\"><em>K. lactis<\/em> Protein Expression Kit<\/a> for the expression of post-translational modified proteins in yeast<\/li>\n<\/ul>\n<h3>Purify tagged proteins with our magnetic beads, columns and resins<\/h3>\n<ul>\n<li><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=s1427\">Nickel spin columns<\/a>,\u00a0<a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=s1423\">magnetic beads<\/a>\u00a0and\u00a0<a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=s1428\">resin<\/a>\u00a0enable rapid purification of His-tagged proteins<\/li>\n<li><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=e8021\">Amylose resins<\/a>\u00a0for purification of MBP-tagged proteins are available in a variety formats (<a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=e8021\">standard<\/a>,\u00a0<a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=e8022\">high flow<\/a>\u00a0and\u00a0<a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=e8035\">magnetic<\/a>)<\/li>\n<li><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=s6651\">Chitin resin<\/a>\u00a0allows for rapid purification of CBD-tagged proteins<\/li>\n<li>Remove affinity tags following your purification with\u00a0<a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=p8112\">TEV Protease<\/a><\/li>\n<\/ul>\n<\/div>\n<\/article>\n<\/div>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row bwd_tbc4vd_show_weekday=&#8221;&#8221; disable_element=&#8221;yes&#8221;][vc_column][vc_column_text]<\/p>\n<div id=\"main_0_wideleftcolumn_0_divContainer\" class=\"line wide pane group\">\n<article class=\"column-left\">\n<div class=\"inner\">\n<p>Protein expression and purification can be very challenging because there is no single approach that is appropriate for every protein or downstream application.<\/p>\n<p>Successful expression can be affected by each individual protein\u2019s solubility, toxicity, need for post-translational modifications or its intrinsic ability to fold. Additionally, the application for which the protein is ultimately to be used may dictate how the protein needs to be expressed or purified. It is therefore important to have a flexible set of tools and methods that can be applied to each unique project.<\/p>\n<p>Each NEB expression technology offers different advantages enabling you to choose the strategy that best suits your protein expression and purification needs. Many of the expression vectors share a compatible polylinker, enabling the gene of interest to be easily shuffled between systems.<\/p>\n<p>Please select your favorite system:<\/p>\n<p>E.coli:<\/p>\n<ul>\n<li><a title=\"pMAL\" href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=E8200\">pMAL\u2122 Protein Fusion and Purification System<\/a><\/li>\n<li><a title=\"IMPACT\" href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=E6901\">IMPACT Kit<\/a><\/li>\n<\/ul>\n<p>Yeast<\/p>\n<ul>\n<li><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=E1000\" target=\"_blank\" rel=\"noopener noreferrer\">K<em>. lactis<\/em><\/a>\u00a0Expression Kit<\/li>\n<\/ul>\n<p>Cell-Free:<\/p>\n<ul>\n<li><a title=\"PURExpress \u2013 In Vitro Protein Expression\" href=\"https:\/\/www.neb-online.de\/en\/neb\/proteinexpression\/purexpress-in-vitro-protein-expression\/\" target=\"_blank\" rel=\"noopener noreferrer\">PURExpress\u00ae<\/a>\u00a0\u2013 a\u00a0cell-free transcription\/translation system reconstituted from purified components<\/li>\n<\/ul>\n<p>Besides optimized competent <a title=\"E. coli Strains for Protein Expression\" href=\"https:\/\/www.neb-online.de\/en\/proteinexpression\/e-coli-strains-for-protein-expression\/\"><em>E. coli<\/em> for protein expression<\/a> you&#8217;ll also find <a href=\"http:\/\/shop.neb-online.de\/4DCGI\/ezshop?hid=47&amp;Sprachnr=2&amp;Suchbegriff=Yeast%20Expression%20Systems&amp;Worldnr=1\" target=\"_blank\" rel=\"noopener noreferrer\"><em>Kluyveromyces lactis<\/em><\/a> (K. lactis, yeast).<\/p>\n<\/div>\n<\/article>\n<\/div>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=&#8221;custom&#8221; accent_color=&#8221;#eb7e2d&#8221;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n<h2>Protein Expression and Purification Selection Chart<\/h2>\n<p>[\/vc_column_text][vc_column_text]<\/p>\n<table>\n<thead>\n<tr style=\"background-color: #f3b281;\">\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"20%\" height=\"40px\">Application<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"25%\" height=\"40px\">Kit<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"55%\" height=\"40px\">Advantages<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<th style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"3\">High-Yield Expression<\/th>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=e8200\">pMAL\u2122 Protein Fusion and Purification System<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; padding-top: 10px; padding-bottom: 10px;\">Substantial yields (up to 100 mg\/L) in more than 75% cases tested; uses the strong P<sub>tac<\/sub> promoter.<\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; background-color: #f4f1e7;\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=e1000\"><em>K. lactis<\/em> Protein Expression Kit<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; padding-top: 10px; padding-bottom: 10px; background-color: #f4f1e7;\">Uses the strong <em>LAC4<\/em> promoter; multiple integrations of plasmid results in higher yield.<\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=e6901\">IMPACT\u2122 Kit<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; padding-top: 10px; padding-bottom: 10px;\">Uses the T7 promoter for high level regulated expression.<\/td>\n<\/tr>\n<tr>\n<th style=\"border-bottom: 1px solid #eb7e2d;\">Cell-Free Expression<\/th>\n<td style=\"border-bottom: 1px solid #eb7e2d; background-color: #f4f1e7;\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=e6800\">PURExpress\u00ae <em>In Vitro<\/em> Protein Synthesis Kit<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; padding-top: 10px; padding-bottom: 10px; background-color: #f4f1e7;\">Quickly generates analytical amounts of protein.<\/td>\n<\/tr>\n<tr>\n<th style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\">Co-Expression of Multiple Proteins<\/th>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=e1000\"><em>K. lactis<\/em> Protein Expression Kit<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; padding-top: 10px; padding-bottom: 10px;\">Easily co-express 2\u20134 proteins.<\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; background-color: #f4f1e7;\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=e6800\">PURExpress\u00ae <em>In Vitro <\/em>Protein Synthesis Kit<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; padding-top: 10px; padding-bottom: 10px; background-color: #f4f1e7;\">Bicistronic constructs or multiple plasmids with appropriate transcription and translation regulatory elements can be used.<\/td>\n<\/tr>\n<tr>\n<th style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\">Enhanced Solubility<\/th>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=e8200\">pMAL\u2122 Protein Fusion and Purification System<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; padding-top: 10px; padding-bottom: 10px;\">Fusion to MBP enhances solubility of proteins in <em>E. coli<\/em>.*<\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; background-color: #f4f1e7;\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=e1000\"><em>K. lactis<\/em> Protein Expression Kit<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; padding-top: 10px; padding-bottom: 10px; background-color: #f4f1e7;\">Utilizes <em>K. lactis<\/em> eukaryotic folding pathway.<\/td>\n<\/tr>\n<tr>\n<th style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"3\">Affinity Tag Chromatography<\/th>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=e6901\">IMPACT\u2122 Kit<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; padding-top: 10px; padding-bottom: 10px;\">Utilizes an intein-chitin binding domain (CBD) tag on either the N- or C- terminus, offering single-step purification.<\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; background-color: #f4f1e7;\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=e8200\">pMAL\u2122 Protein Fusion and Purification System<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; padding-top: 10px; padding-bottom: 10px; background-color: #f4f1e7;\">Fusion to MBP allows for purification on amylose resin.<\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=e1000\"><em>K. lactis<\/em> Protein Expression Kit<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; padding-top: 10px; padding-bottom: 10px;\">Vectors are sold separately that generate fusions to MBP allowing for purification on amylose restin.<\/td>\n<\/tr>\n<tr>\n<th style=\"border-bottom: 1px solid #eb7e2d;\">Post-translational Modification<\/th>\n<td style=\"border-bottom: 1px solid #eb7e2d; background-color: #f4f1e7;\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=e1000\"><em>K. lactis<\/em> Protein Expression Kit<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; padding-top: 10px; padding-bottom: 10px; background-color: #f4f1e7;\">Secretion of both <em>N-<\/em> and <em>O-<\/em> glycosylated proteins.<\/td>\n<\/tr>\n<tr>\n<th style=\"border-bottom: 1px solid #eb7e2d;\">Periplasmic Expression<\/th>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=e8200\">pMAL\u2122 Protein Fusion and Purification System<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; padding-top: 10px; padding-bottom: 10px;\">Periplasmic expression enhances folding of proteins with disulfide bonds.<\/td>\n<\/tr>\n<tr>\n<th style=\"border-bottom: 1px solid #eb7e2d;\">Secreted Expression<\/th>\n<td style=\"border-bottom: 1px solid #eb7e2d; background-color: #f4f1e7;\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=e1000\"><em>K. lactis<\/em> Protein Expression Kit<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; padding-top: 10px; padding-bottom: 10px; background-color: #f4f1e7;\">Eliminates cell lysis step, simplifying purification.<\/td>\n<\/tr>\n<tr>\n<th style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"4\">Toxic Proteins<\/th>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=e1000\"><em>K. lactis<\/em> Protein Expression Kit<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; padding-top: 10px; padding-bottom: 10px;\">Secretion of protein from the cell.<\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; background-color: #f4f1e7;\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=e6901\">IMPACT\u2122 Kit<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; padding-top: 10px; padding-bottom: 10px; background-color: #f4f1e7;\">Can express the toxic gene in two pieces and ligate proteins together using intein mediated protein ligation (IPL).<\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=e8200\">pMAL\u2122 Protein Fusion and Purification System<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; padding-top: 10px; padding-bottom: 10px;\">Can export toxic proteins into periplasmic space.<\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; background-color: #f4f1e7;\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=e6800\">PURExpress\u00ae <em>In Vitro<\/em> Protein Synthesis Kit<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; padding-top: 10px; padding-bottom: 10px; background-color: #f4f1e7;\">Cell-free environment not affected by &#8220;toxicity&#8221; of expressed protein.<\/td>\n<\/tr>\n<tr>\n<th style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\">Protein Modification<\/th>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=e6901\">IMPACT\u2122 Kit<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; padding-top: 10px; padding-bottom: 10px;\">Generates proteins with reactive ends (N-terminal cysteine and\/or C-terminal thioester) allowing for labeling or ligation of proteins or peptides.<\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; background-color: #f4f1e7;\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=e6800\">PURExpress\u00ae <em>In Vitro<\/em> Protein Synthesis Kit<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; padding-top: 10px; padding-bottom: 10px; background-color: #f4f1e7;\">Allows introduction of modified, unnatural or labeled amino acids.<\/td>\n<\/tr>\n<tr>\n<th style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\">No Additional Amino Acid Residues<\/th>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=e6901\">IMPACT\u2122 Kit<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; padding-top: 10px; padding-bottom: 10px;\">Start of native protein is fused adjacent to site of intein cleavage.<\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; background-color: #f4f1e7;\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=e8200\">pMAL\u2122 Protein Fusion and Purification System<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; padding-top: 10px; padding-bottom: 10px; background-color: #f4f1e7;\">Start of protein is fused adjacent to protease site.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row][vc_column][vc_basic_grid post_type=&#8221;post&#8221; max_items=&#8221;10&#8243; element_width=&#8221;12&#8243; gap=&#8221;15&#8243; item=&#8221;4123&#8243; taxonomies=&#8221;75&#8243; grid_id=&#8221;vc_gid:1631603551263-06b345da-ccba-6&#8243;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text] It\u2019s a matter of expression. Protein expression can be a very complex, multi-factorial process. Each protein requires a specific intracellular environment to correctly achieve its secondary and tertiary structures. Proteins may also require post-translational modifications or insertion into a cellular membrane for proper function. Other proteins, once&#8230;  <a class=\"excerpt-read-more\" href=\"https:\/\/www.neb-online.de\/en\/proteinexpression\/\" title=\"Read Protein Expression\">Read more &raquo;<\/a><\/p>\n","protected":false},"author":2,"featured_media":42170,"parent":0,"menu_order":0,"comment_status":"open","ping_status":"closed","template":"page-sidebar.php","meta":{"footnotes":""},"class_list":["post-2254","page","type-page","status-publish","has-post-thumbnail","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.1.1 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Protein Expression - New England Biolabs GmbH<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.neb-online.de\/en\/proteinexpression\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Protein Expression - New England Biolabs GmbH\" \/>\n<meta property=\"og:description\" content=\"[vc_row][vc_column][vc_basic_grid post_type=&#8221;post&#8221; max_items=&#8221;10&#8243; element_width=&#8221;12&#8243; gap=&#8221;15&#8243; item=&#8221;4123&#8243; taxonomies=&#8221;75&#8243; grid_id=&#8221;vc_gid:1631603551263-06b345da-ccba-6&#8243;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text] It\u2019s a matter of expression. Protein expression can be a very complex, multi-factorial process. Each protein requires a specific intracellular environment to correctly achieve its secondary and tertiary structures. Proteins may also require post-translational modifications or insertion into a cellular membrane for proper function. Other proteins, once... Read more &raquo;\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.neb-online.de\/en\/proteinexpression\/\" \/>\n<meta property=\"og:site_name\" content=\"New England Biolabs GmbH\" \/>\n<meta property=\"article:modified_time\" content=\"2021-09-14T07:13:14+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2020\/01\/Homebanner_Expression_Express.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1080\" \/>\n\t<meta property=\"og:image:height\" content=\"284\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"5 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.neb-online.de\/en\/proteinexpression\/\",\"url\":\"https:\/\/www.neb-online.de\/en\/proteinexpression\/\",\"name\":\"Protein Expression - New England Biolabs GmbH\",\"isPartOf\":{\"@id\":\"https:\/\/www.neb-online.de\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/www.neb-online.de\/en\/proteinexpression\/#primaryimage\"},\"image\":{\"@id\":\"https:\/\/www.neb-online.de\/en\/proteinexpression\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2020\/01\/Homebanner_Expression_Express.jpg\",\"datePublished\":\"2015-04-29T08:02:46+00:00\",\"dateModified\":\"2021-09-14T07:13:14+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/www.neb-online.de\/en\/proteinexpression\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/www.neb-online.de\/en\/proteinexpression\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/www.neb-online.de\/en\/proteinexpression\/#primaryimage\",\"url\":\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2020\/01\/Homebanner_Expression_Express.jpg\",\"contentUrl\":\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2020\/01\/Homebanner_Expression_Express.jpg\",\"width\":1080,\"height\":284},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/www.neb-online.de\/en\/proteinexpression\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/www.neb-online.de\/en\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Protein Expression\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/www.neb-online.de\/#website\",\"url\":\"https:\/\/www.neb-online.de\/\",\"name\":\"New England Biolabs GmbH\",\"description\":\"Enabling Technologies in the Life Sciences\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/www.neb-online.de\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Protein Expression - New England Biolabs GmbH","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.neb-online.de\/en\/proteinexpression\/","og_locale":"en_US","og_type":"article","og_title":"Protein Expression - New England Biolabs GmbH","og_description":"[vc_row][vc_column][vc_basic_grid post_type=&#8221;post&#8221; max_items=&#8221;10&#8243; element_width=&#8221;12&#8243; gap=&#8221;15&#8243; item=&#8221;4123&#8243; taxonomies=&#8221;75&#8243; grid_id=&#8221;vc_gid:1631603551263-06b345da-ccba-6&#8243;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text] It\u2019s a matter of expression. Protein expression can be a very complex, multi-factorial process. Each protein requires a specific intracellular environment to correctly achieve its secondary and tertiary structures. Proteins may also require post-translational modifications or insertion into a cellular membrane for proper function. Other proteins, once... 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