{"id":2256,"date":"2015-04-29T08:08:30","date_gmt":"2015-04-29T08:08:30","guid":{"rendered":"http:\/\/www.neb-online.de\/?page_id=2256"},"modified":"2023-12-19T14:12:35","modified_gmt":"2023-12-19T13:12:35","slug":"nebxpress-cell-free-e-coli-proteine-synthesis-system","status":"publish","type":"page","link":"https:\/\/www.neb-online.de\/en\/proteinexpression\/nebxpress-cell-free-e-coli-proteine-synthesis-system\/","title":{"rendered":"NEBExpress Cell-free E. coli Protein Synthesis System"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column][vc_basic_grid post_type=&#8221;post&#8221; max_items=&#8221;10&#8243; element_width=&#8221;12&#8243; gap=&#8221;15&#8243; item=&#8221;4123&#8243; taxonomies=&#8221;75&#8243; grid_id=&#8221;vc_gid:1702991488972-05392000-8020-9&#8243;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n<h2>New: NEBExpress Cell-free <em>E. coli<\/em> Protein Synthesis System<\/h2>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]The NEBExpress Cell-free <em>E. coli<\/em> Protein Synthesis System is a coupled transcription\/translation system designed to synthesize proteins encoded by a DNA template under the control of a T7 RNA Polymerase promoter. The system offers high expression levels, the ability to produce high molecular weight proteins, scalability, and is cost-effective for high throughput expression applications. The speed and robustness of the system facilitates protein synthesis in applications such as protein engineering, mutagenesis studies and enzyme screening. In addition, it can be used to generate proteins for biophysical and structure-function analyses.[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243;]<div class=\"vbox\">\n\t\t\t\t<span class=\"vhead\">Advantages:<\/span>\n\t\t\t\t\t<ul class=\"vlist\">\n\t\t\t\t\t\t<li><span>Quickly generate analytical amounts of protein for further characterization<br \/><\/span><\/li><li><span>Synthesizes various target proteins ranging in size from 17 to 230 kDa<br \/><\/span><\/li><li><span>Templates can be either plasmid DNA, linear DNA, or mRNA<br \/><\/span><\/li><li><span>Minimal protease activity ensures stability of desired target protein<br \/><\/span><\/li><li><span>Flexible reaction conditions<br \/><\/span><\/li><li><span>Reactions can be directly loaded onto an SDS-PAGE gel without the need for acetone or TCA precipitation<\/span><\/li>\n\t\t\t\t\t<\/ul>\n\t\t\t<\/div>[\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]The NEBExpress Cell-free <em>E. coli<\/em> Protein Synthesis System contains all the components required for protein synthesis, except for the target template DNA. It is a combination of a highly active cell extract from a genetically engineered strain, a reaction buffer, and an optimized T7 RNA Polymerase, which together yield robust expression of a wide variety of protein targets ranging from 17 to 230 KDa.[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_text_separator title=&#8221;Video: NEBExpress Cell-free E. Coli&#8221; color=&#8221;custom&#8221; accent_color=&#8221;#eb7e2d&#8221;][vc_video link=&#8221;https:\/\/youtu.be\/XhKo5oPSsH0&#8243;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]Protein synthesis is achieved with a short incubation and synthesized protein is compatible with downstream purification or analysis by SDS-PAGE, Western Blot or direct functional assay. The novel formulation of this system allows samples to be loaded directly onto SDS-PAGE, without the need for acetone or TCA precipitation.\u00a0 Additionally, synthesized protein can be isolated from the reaction mixture using affinity purification techniques, such as immobilized metal affinity chromatography (IMAC), for further structural and\/or functional characterization.[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_single_image image=&#8221;21263&#8243; img_size=&#8221;full&#8221;][\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<span class=\"bildunterschrift\"><strong>Protein synthesis using the NEBExpressCell-free E. coli Protein Synthesis System<\/strong><br \/>\n50 \u00b5l reactions containing 250 ng template DNA were incubated at 37\u00b0C for 3 hours. 2 \u00b5l of each reaction were analyzed by SDS-PAGE using a 10\u201320% Tris-glycine gel. The red dot indicates the protein of interest. M = Unstained Protein Standard, Broad range (NEB\u00a0#P7717), \u201cNeg\u201d = negative control, no DNA.<\/span>[\/vc_column_text][\/vc_column][\/vc_row][vc_row bwd_show_when_type=&#8221;before&#8221; bwd_show_when_date=&#8221;12\/31\/2023 23:00&#8243; bwd_tbc4vd_show_weekday=&#8221;&#8221; el_id=&#8221;purexpress&#8221;][vc_column][vc_column_text]<\/p>\n<h2>Available NEBExpress Cell-free products:<\/h2>\n<table style=\"width: 760px;\" border=\"0\" cellpadding=\"5\">\n<tbody>\n<tr style=\"background-color: #f3f3f3; font-size: 16px;\">\n<th style=\"border-bottom: solid #eb7e2d;\" align=\"left\" width=\"400\" height=\"40\">PRODUCT<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"88\">Prod. #<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"100\">SIZE<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" colspan=\"3\">PRICE<\/th>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\">NEBExpress Cell-free <em>E. coli<\/em> Protein Synthesis System<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E5360S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">10 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>214 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/E5360\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/E5360\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E5360L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>1.732 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/E5360\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/E5360\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"padding-top: 50px;\" colspan=\"6\"><strong>Passend dazu:<\/strong><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">NEBExpress GamS Nuclease Inhibitor<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">P0744S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">75 \u00b5g<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>139 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/P0744S\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/P0744S\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">PURExpress Disulfide Bond Enhancer<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E6820S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">50 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>252 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/E6820\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/E6820\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p style=\"text-align: right;\"><span class=\"bildunterschrift\">Stand: 03.01.2022<\/span><\/p>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row bwd_show_when_type=&#8221;after&#8221; bwd_show_when_date=&#8221;12\/31\/2023 23:00&#8243; bwd_tbc4vd_show_weekday=&#8221;&#8221;][vc_column][vc_column_text]<\/p>\n<h2>Available NEBExpress Cell-free products:<\/h2>\n<table style=\"width: 760px;\" border=\"0\" cellpadding=\"5\">\n<tbody>\n<tr style=\"background-color: #f3f3f3; font-size: 16px;\">\n<th style=\"border-bottom: solid #eb7e2d;\" align=\"left\" width=\"400\" height=\"40\">PRODUCT<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"88\">Prod. #<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"100\">SIZE<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" colspan=\"2\">INFO<\/th>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\">NEBExpress Cell-free <em>E. coli<\/em> Protein Synthesis System<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E5360S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">10 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/E5360\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/E5360\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E5360L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/E5360\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/E5360\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"padding-top: 50px;\" colspan=\"6\"><strong>Passend dazu:<\/strong><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">NEBExpress GamS Nuclease Inhibitor<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">P0744S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">75 \u00b5g<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/P0744S\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/P0744S\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">PURExpress Disulfide Bond Enhancer<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E6820S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">50 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/E6820\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/E6820\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p style=\"text-align: right;\"><span class=\"bildunterschrift\">Stand: 03.01.2024<\/span><\/p>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row bwd_tbc4vd_show_weekday=&#8221;&#8221; disable_element=&#8221;yes&#8221;][vc_column][vc_column_text]<\/p>\n<h2>Protein Expression and Purification Selection Chart<\/h2>\n<p>[\/vc_column_text][vc_column_text]<\/p>\n<table>\n<thead>\n<tr style=\"background-color: #f3b281;\">\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"20%\" height=\"40px\">Application<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"25%\" height=\"40px\">Kit<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"55%\" height=\"40px\">Advantages<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<th style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"3\">High-Yield Expression<\/th>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=e8200\">pMAL\u2122 Protein Fusion and Purification System<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; padding-top: 10px; padding-bottom: 10px;\">Substantial yields (up to 100 mg\/L) in more than 75% cases tested; uses the strong P<sub>tac<\/sub> promoter.<\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; background-color: #f4f1e7;\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=e1000\"><em>K. lactis<\/em> Protein Expression Kit<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; padding-top: 10px; padding-bottom: 10px; background-color: #f4f1e7;\">Uses the strong <em>LAC4<\/em> promoter; multiple integrations of plasmid results in higher yield.<\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=e6901\">IMPACT\u2122 Kit<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; padding-top: 10px; padding-bottom: 10px;\">Uses the T7 promoter for high level regulated expression.<\/td>\n<\/tr>\n<tr>\n<th style=\"border-bottom: 1px solid #eb7e2d;\">Cell-Free Expression<\/th>\n<td style=\"border-bottom: 1px solid #eb7e2d; background-color: #f4f1e7;\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=e6800\">PURExpress\u00ae <em>In Vitro<\/em> Protein Synthesis Kit<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; padding-top: 10px; padding-bottom: 10px; background-color: #f4f1e7;\">Quickly generates analytical amounts of protein.<\/td>\n<\/tr>\n<tr>\n<th style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\">Co-Expression of Multiple Proteins<\/th>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=e1000\"><em>K. lactis<\/em> Protein Expression Kit<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; padding-top: 10px; padding-bottom: 10px;\">Easily co-express 2\u20134 proteins.<\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; background-color: #f4f1e7;\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=e6800\">PURExpress\u00ae <em>In Vitro <\/em>Protein Synthesis Kit<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; padding-top: 10px; padding-bottom: 10px; background-color: #f4f1e7;\">Bicistronic constructs or multiple plasmids with appropriate transcription and translation regulatory elements can be used.<\/td>\n<\/tr>\n<tr>\n<th style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\">Enhanced Solubility<\/th>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=e8200\">pMAL\u2122 Protein Fusion and Purification System<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; padding-top: 10px; padding-bottom: 10px;\">Fusion to MBP enhances solubility of proteins in <em>E. coli<\/em>.*<\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; background-color: #f4f1e7;\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=e1000\"><em>K. lactis<\/em> Protein Expression Kit<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; padding-top: 10px; padding-bottom: 10px; background-color: #f4f1e7;\">Utilizes <em>K. lactis<\/em> eukaryotic folding pathway.<\/td>\n<\/tr>\n<tr>\n<th style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"3\">Affinity Tag Chromatography<\/th>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=e6901\">IMPACT\u2122 Kit<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; padding-top: 10px; padding-bottom: 10px;\">Utilizes an intein-chitin binding domain (CBD) tag on either the N- or C- terminus, offering single-step purification.<\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; background-color: #f4f1e7;\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=e8200\">pMAL\u2122 Protein Fusion and Purification System<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; padding-top: 10px; padding-bottom: 10px; background-color: #f4f1e7;\">Fusion to MBP allows for purification on amylose resin.<\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=e1000\"><em>K. lactis<\/em> Protein Expression Kit<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; padding-top: 10px; padding-bottom: 10px;\">Vectors are sold separately that generate fusions to MBP allowing for purification on amylose restin.<\/td>\n<\/tr>\n<tr>\n<th style=\"border-bottom: 1px solid #eb7e2d;\">Post-translational Modification<\/th>\n<td style=\"border-bottom: 1px solid #eb7e2d; background-color: #f4f1e7;\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=e1000\"><em>K. lactis<\/em> Protein Expression Kit<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; padding-top: 10px; padding-bottom: 10px; background-color: #f4f1e7;\">Secretion of both <em>N-<\/em> and <em>O-<\/em> glycosylated proteins.<\/td>\n<\/tr>\n<tr>\n<th style=\"border-bottom: 1px solid #eb7e2d;\">Periplasmic Expression<\/th>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=e8200\">pMAL\u2122 Protein Fusion and Purification System<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; padding-top: 10px; padding-bottom: 10px;\">Periplasmic expression enhances folding of proteins with disulfide bonds.<\/td>\n<\/tr>\n<tr>\n<th style=\"border-bottom: 1px solid #eb7e2d;\">Secreted Expression<\/th>\n<td style=\"border-bottom: 1px solid #eb7e2d; background-color: #f4f1e7;\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=e1000\"><em>K. lactis<\/em> Protein Expression Kit<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; padding-top: 10px; padding-bottom: 10px; background-color: #f4f1e7;\">Eliminates cell lysis step, simplifying purification.<\/td>\n<\/tr>\n<tr>\n<th style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"4\">Toxic Proteins<\/th>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=e1000\"><em>K. lactis<\/em> Protein Expression Kit<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; padding-top: 10px; padding-bottom: 10px;\">Secretion of protein from the cell.<\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; background-color: #f4f1e7;\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=e6901\">IMPACT\u2122 Kit<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; padding-top: 10px; padding-bottom: 10px; background-color: #f4f1e7;\">Can express the toxic gene in two pieces and ligate proteins together using intein mediated protein ligation (IPL).<\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=e8200\">pMAL\u2122 Protein Fusion and Purification System<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; padding-top: 10px; padding-bottom: 10px;\">Can export toxic proteins into periplasmic space.<\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; background-color: #f4f1e7;\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=e6800\">PURExpress\u00ae <em>In Vitro<\/em> Protein Synthesis Kit<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; padding-top: 10px; padding-bottom: 10px; background-color: #f4f1e7;\">Cell-free environment not affected by &#8220;toxicity&#8221; of expressed protein.<\/td>\n<\/tr>\n<tr>\n<th style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\">Protein Modification<\/th>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=e6901\">IMPACT\u2122 Kit<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; padding-top: 10px; padding-bottom: 10px;\">Generates proteins with reactive ends (N-terminal cysteine and\/or C-terminal thioester) allowing for labeling or ligation of proteins or peptides.<\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; background-color: #f4f1e7;\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=e6800\">PURExpress\u00ae <em>In Vitro<\/em> Protein Synthesis Kit<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; padding-top: 10px; padding-bottom: 10px; background-color: #f4f1e7;\">Allows introduction of modified, unnatural or labeled amino acids.<\/td>\n<\/tr>\n<tr>\n<th style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"2\">No Additional Amino Acid Residues<\/th>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=e6901\">IMPACT\u2122 Kit<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; padding-top: 10px; padding-bottom: 10px;\">Start of native protein is fused adjacent to site of intein cleavage.<\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; background-color: #f4f1e7;\"><a href=\"https:\/\/www.neb-online.de\/shop\/neb_en_product.php?productnumber=e8200\">pMAL\u2122 Protein Fusion and Purification System<\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; padding-top: 10px; padding-bottom: 10px; background-color: #f4f1e7;\">Start of protein is fused adjacent to protease site.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row][vc_column][vc_basic_grid post_type=&#8221;post&#8221; max_items=&#8221;10&#8243; element_width=&#8221;12&#8243; gap=&#8221;15&#8243; item=&#8221;4123&#8243; taxonomies=&#8221;75&#8243; grid_id=&#8221;vc_gid:1702991488972-05392000-8020-9&#8243;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text] New: NEBExpress Cell-free E. coli Protein Synthesis System [\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]The NEBExpress Cell-free E. coli Protein Synthesis System is a coupled transcription\/translation system designed to synthesize proteins encoded by a DNA template under the control of a T7 RNA Polymerase promoter. The system offers high expression levels, the&#8230;  <a class=\"excerpt-read-more\" href=\"https:\/\/www.neb-online.de\/en\/proteinexpression\/nebxpress-cell-free-e-coli-proteine-synthesis-system\/\" title=\"Read NEBExpress Cell-free E. coli Protein Synthesis System\">Read more &raquo;<\/a><\/p>\n","protected":false},"author":2,"featured_media":42170,"parent":2254,"menu_order":0,"comment_status":"open","ping_status":"closed","template":"page-sidebar.php","meta":{"footnotes":""},"class_list":["post-2256","page","type-page","status-publish","has-post-thumbnail","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.1.1 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>NEBExpress Cell-free E. coli Protein Synthesis System - New England Biolabs GmbH<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.neb-online.de\/en\/proteinexpression\/nebxpress-cell-free-e-coli-proteine-synthesis-system\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"NEBExpress Cell-free E. coli Protein Synthesis System - New England Biolabs GmbH\" \/>\n<meta property=\"og:description\" content=\"[vc_row][vc_column][vc_basic_grid post_type=&#8221;post&#8221; max_items=&#8221;10&#8243; element_width=&#8221;12&#8243; gap=&#8221;15&#8243; item=&#8221;4123&#8243; taxonomies=&#8221;75&#8243; grid_id=&#8221;vc_gid:1702991488972-05392000-8020-9&#8243;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text] New: NEBExpress Cell-free E. coli Protein Synthesis System [\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]The NEBExpress Cell-free E. coli Protein Synthesis System is a coupled transcription\/translation system designed to synthesize proteins encoded by a DNA template under the control of a T7 RNA Polymerase promoter. 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