{"id":25769,"date":"2020-11-09T14:39:37","date_gmt":"2020-11-09T13:39:37","guid":{"rendered":"https:\/\/www.neb-online.de\/next-generation-sequencing\/multiplexing-strategien-mit-den-nebnext-multiplex-oligos-for-illumina\/"},"modified":"2024-01-16T16:09:31","modified_gmt":"2024-01-16T15:09:31","slug":"multiplexing-strategien-mit-den-nebnext-multiplex-oligos-for-illumina","status":"publish","type":"page","link":"https:\/\/www.neb-online.de\/en\/next-generation-sequencing\/multiplexing-strategien-mit-den-nebnext-multiplex-oligos-for-illumina\/","title":{"rendered":"Multiplexing Strategy with NEBNext Oligos\/Adaptors"},"content":{"rendered":"

[vc_row][vc_column][vc_basic_grid post_type=”post” max_items=”10″ element_width=”12″ gap=”15″ item=”4123″ grid_id=”vc_gid:1705417750123-0625a967-bd80-6″ taxonomies=”128″][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]Find the best Oligo Strategy for your experiment!
\n<\/b>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_row_inner][vc_column_inner][vc_column_text]<\/p>\n

NEBNext Multiplex Oligos Selection Chart<\/h2>\n\n\n\n\n\n\n\n\n\n\n\n\n
<\/th>\nSingle Index <\/span>\"In<\/th>\nDual Index<\/span> \"In<\/th>\nUnique Dual Index<\/span> \"In<\/th>\nUnique Dual Index UMIs<\/span>
\n\"In<\/th>\n<\/tr>\n
NEB Products<\/span><\/td>\nNEB #E7335<\/span><\/a>,
\nNEB #E7500<\/a>,<\/span><\/span>
\nNEB #E7710<\/a><\/span>,
\nNEB #E7730<\/a><\/span>,
\nNEB #E6609<\/span><\/a><\/span><\/td>\n		NEB #E7600<\/span><\/a>,
\nNEB #E7780<\/span><\/a><\/span><\/td>\n		NEB #E6440<\/span><\/a> (Set\u00a01)
\nNEB #E6442<\/span><\/a> (Set\u00a02)
\nNEB #E6444 <\/a><\/span>(Set\u00a03)
\nNEB #E6446<\/span><\/a> (Set\u00a04)
\nNEB #E6448<\/span><\/a> (Set\u00a05)
\n<\/span><\/span>NEB #E7140<\/span><\/a> (EM-Seq<\/span>)<\/span><\/td>\n		NEB #E7395<\/span><\/a>,
\nNEB #E7874<\/span><\/a>,
\nNEB #E7876<\/span><\/a>,
\nNEB #E7878<\/span><\/a><\/span><\/td>\n<\/tr>\n
Contains UMI<\/td>\nNo<\/td>\nNo<\/td>\nNo<\/td>\nYes<\/td>\n<\/tr>\n
Addresses index hopping<\/td>\nNo<\/td>\nNo<\/td>\nYes<\/td>\nYes<\/td>\n<\/tr>\n
Indexing Strategy<\/td>\nIndex Primer<\/td>\nIndex Primer<\/td>\nIndex Primer<\/td>\nIndex Adaptor<\/td>\n<\/tr>\n
Applications<\/td>\nDNA-seq, RNA-seq
\n(except Small RNA)<\/td>\n		DNA-seq, RNA-seq
\n(except Small RNA)<\/td>\n		DNA-seq, RNA-seq
\n(except Small RNA)<\/td>\n		PCR-free DNA-seq, RNA-seq
\n(except Small RNA)<\/td>\n<\/tr>\n
Number of indices for multiplexing<\/td>\nup to 96<\/td>\nup to 384<\/td>\nup to 480<\/td>\nup to 96<\/td>\n<\/tr>\n
Compatible with EM-seq<\/td>\nYes*<\/td>\nYes*<\/td>\nYes*<\/td>\nNo<\/td>\n<\/tr>\n
Compatible with EpiMark bisulfite sequencing<\/td>\nYes**<\/td>\nYes**<\/td>\nYes**<\/td>\nNo**<\/td>\n<\/tr>\n
Number of sets available; formats and indices available<\/td>\nFive;<\/strong> Sets 1-4 (12 indices\/set): Individual vials 96 Index: Premixed plate<\/td>\nTwo;<\/strong> individual vials containing 8 i5 primers and 12 i7 primers for combinatorial mixing<\/td>\nFive;<\/strong> 96 index combinations, premixed in foil-sealed 96-well plates; Including a version for EM-seq (up to 120 indices, either 96-well plate or 24 vial format)<\/td>\nFour;<\/strong> 96 index adaptor combinations, premixed in foil-sealed 96 well plate (DNA-seq OR RNA-seq) and primers<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

* Requires the use of the EM-seq Adaptor; Single, dual and unique dual index are all compatible; NEB recommends using the Unique Dual Index Primers found in the NEBNext Enzymatic\u00a0Methyl-seq Kit (NEB #E7120<\/a><\/span>) or the NEBNext Multiplex Oligos for EM-seq (NEB #E7140<\/span><\/a>), both supplied with the NEBNext EM-seq Adaptor; For higher levels of multiplexing,\u00a0Unique Dual Index Primers Sets 3 and 4\u00a0 (NEB #E6444<\/a><\/span> and #E6446<\/a><\/span>) are also validated for EM-seq.<\/p>\n

**\u00a0 Requires use of NEBNext EM-seq adaptor from NEBNext Multiplex Oligos for Enzymatic Methyl-seq (Unique Dual Index Primer Pairs, #E7140<\/a><\/span>S\/L or NEBNext methylated adaptor from NEBNext Multiplex Oligos for Illumina (Methylated Adaptor, Index Primers Set 1, #E7535<\/a><\/span>S\/L) .[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row bwd_show_when_type=”before” bwd_show_when_date=”12\/31\/2023 23:00″ bwd_tbc4vd_show_weekday=””][vc_column][vc_column_text]<\/p>\n

Available NEBNext (Mulitplex-) Oligos for Illumina<\/h2>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
PRDOUCT<\/th>\nArt. #<\/th>\nSIZE<\/th>\nPRICE<\/th>\n<\/tr>\n
NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs Set 1)<\/td>\nE6440S<\/td>\n96 rxns<\/td>\n578 \u20ac<\/strong><\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
E6440L<\/td>\n384 rxns<\/td>\n2.080 \u20ac<\/strong><\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs Set 2)<\/td>\nE6442S<\/td>\n96 rxns<\/td>\n578 \u20ac<\/strong><\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
E6442L<\/td>\n384 rxns<\/td>\n2.080 \u20ac<\/strong><\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs Set 3)<\/td>\nE6444S<\/td>\n96 rxns<\/td>\n578 \u20ac<\/strong><\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
E6444L<\/td>\n384 rxns<\/td>\n2.080 \u20ac<\/strong><\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs Set 4)<\/td>\nE6446S<\/td>\n96 rxns<\/td>\n578 \u20ac<\/strong><\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
E6446L<\/td>\n384 rxns<\/td>\n2.080 \u20ac<\/strong><\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs Set 5)<\/td>\nE6448S<\/td>\n96 rxns<\/td>\n578 \u20ac<\/strong><\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
E6448L<\/td>\n384 rxns<\/td>\n2.080 \u20ac<\/strong><\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
NEBNext Multiplex Oligos for Illumina (Index Primer Set 1)<\/td>\nE7335S<\/td>\n24 rxns<\/td>\n114 \u20ac<\/strong><\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
E7335L<\/td>\n96 rxns<\/td>\n417 \u20ac<\/strong><\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
NEBNext Multiplex Oligos for Illumina (Index Primer Set 2)<\/td>\nE7500S<\/td>\n24 rxns<\/td>\n114 \u20ac<\/strong><\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
E7500L<\/td>\n96 rxns<\/td>\n417 \u20ac<\/strong><\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
NEBNext Multiplex Oligos for Illumina (Index Primer Set 3)<\/td>\nE7710S<\/td>\n24 rxns<\/td>\n114 \u20ac<\/strong><\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
E7710L<\/td>\n96 rxns<\/td>\n417 \u20ac<\/strong><\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
NEBNext Multiplex Oligos for Illumina (Index Primer Set 4)<\/td>\nE7730S<\/td>\n24 rxns<\/td>\n114 \u20ac<\/strong><\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
E7730L<\/td>\n96 rxns<\/td>\n417 \u20ac<\/strong><\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
NEBNext Multiplex Oligos for Illumina (96 Index Primers)<\/td>\nE6609S<\/td>\n96 rxns<\/td>\n726 \u20ac<\/strong><\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
E6609L<\/td>\n384 rxns<\/td>\n2.904 \u20ac<\/strong><\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
NEBNext Multiplex Oligos for Illumina (Dual Index Set 1)<\/td>\nE7600S<\/td>\n96 rxns<\/td>\n498 \u20ac<\/strong><\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
NEBNext Multiplex Oligos for Illumina (Dual Index Set 2)<\/td>\nE7780S<\/td>\n96 rxns<\/td>\n498 \u20ac<\/strong><\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
NEBNext Multiplex Oligos for Enzymatic Methyl-seq (Unique Dual Index Primer Pairs)<\/td>\nE7140S<\/td>\n24 rxns<\/td>\n146 \u20ac<\/strong><\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
E7140L<\/td>\n96 rxns<\/td>\n578 \u20ac<\/strong><\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
NEBNext Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors DNA Set 1)<\/td>\nE7395S<\/td>\n96 rxns<\/td>\n728 \u20ac<\/strong><\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
E7395L<\/td>\n384 rxns<\/td>\n2.766 \u20ac<\/strong><\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
NEBNext Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors RNA Set 1)<\/td>\nE7416S<\/td>\n96 rxns<\/td>\n700 \u20ac<\/strong><\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
E7416L<\/td>\n384 rxns<\/td>\n2.656 \u20ac<\/strong><\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

As of: 01.01.2023<\/i><\/span>[\/vc_column_text][\/vc_column][\/vc_row][vc_row bwd_show_when_type=”after” bwd_show_when_date=”12\/31\/2023 23:00″ bwd_tbc4vd_show_weekday=””][vc_column][vc_column_text]<\/p>\n

Available NEBNext (Mulitplex-) Oligos for Illumina<\/h2>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
PRDOUCT<\/th>\nArt. #<\/th>\nSIZE<\/th>\nINFO<\/th>\n<\/tr>\n
NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs Set 1)<\/td>\nE6440S<\/td>\n96 rxns<\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
E6440L<\/td>\n384 rxns<\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs Set 2)<\/td>\nE6442S<\/td>\n96 rxns<\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
E6442L<\/td>\n384 rxns<\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs Set 3)<\/td>\nE6444S<\/td>\n96 rxns<\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
E6444L<\/td>\n384 rxns<\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs Set 4)<\/td>\nE6446S<\/td>\n96 rxns<\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
E6446L<\/td>\n384 rxns<\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs Set 5)<\/td>\nE6448S<\/td>\n96 rxns<\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
E6448L<\/td>\n384 rxns<\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
NEBNext Multiplex Oligos for Illumina (Index Primer Set 1)<\/td>\nE7335S<\/td>\n24 rxns<\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
E7335L<\/td>\n96 rxns<\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
NEBNext Multiplex Oligos for Illumina (Index Primer Set 2)<\/td>\nE7500S<\/td>\n24 rxns<\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
E7500L<\/td>\n96 rxns<\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
NEBNext Multiplex Oligos for Illumina (Index Primer Set 3)<\/td>\nE7710S<\/td>\n24 rxns<\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
E7710L<\/td>\n96 rxns<\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
NEBNext Multiplex Oligos for Illumina (Index Primer Set 4)<\/td>\nE7730S<\/td>\n24 rxns<\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
E7730L<\/td>\n96 rxns<\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
NEBNext Multiplex Oligos for Illumina (96 Index Primers)<\/td>\nE6609S<\/td>\n96 rxns<\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
E6609L<\/td>\n384 rxns<\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
NEBNext Multiplex Oligos for Illumina (Dual Index Set 1)<\/td>\nE7600S<\/td>\n96 rxns<\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
NEBNext Multiplex Oligos for Illumina (Dual Index Set 2)<\/td>\nE7780S<\/td>\n96 rxns<\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
NEBNext Multiplex Oligos for Enzymatic Methyl-seq (Unique Dual Index Primer Pairs)<\/td>\nE7140S<\/td>\n24 rxns<\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
E7140L<\/td>\n96 rxns<\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
NEBNext Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors DNA Set 1)<\/td>\nE7395S<\/td>\n96 rxns<\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
E7395L<\/td>\n384 rxns<\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
NEBNext Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors RNA Set 1)<\/td>\nE7416S<\/td>\n96 rxns<\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n
E7416L<\/td>\n384 rxns<\/td>\n\"In<\/a><\/td>\n\"Produktinformationen\"<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Stand: 01.01.2024<\/i><\/span>
\nAs of: 01.01.2024<\/i><\/span>[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"

[vc_row][vc_column][vc_basic_grid post_type=”post” max_items=”10″ element_width=”12″ gap=”15″ item=”4123″ grid_id=”vc_gid:1705417750123-0625a967-bd80-6″ taxonomies=”128″][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]Find the best Oligo Strategy for your experiment! [\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_row_inner][vc_column_inner][vc_column_text] NEBNext Multiplex Oligos Selection Chart Single Index Dual Index Unique Dual Index Unique Dual Index UMIs NEB Products NEB #E7335, NEB #E7500, NEB #E7710, NEB #E7730, NEB #E6609 NEB #E7600, NEB #E7780 NEB #E6440 (Set\u00a01) NEB #E6442 (Set\u00a02) NEB… Read more »<\/a><\/p>\n","protected":false},"author":4,"featured_media":25770,"parent":2170,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-sidebar.php","meta":{"footnotes":""},"class_list":["post-25769","page","type-page","status-publish","has-post-thumbnail","hentry"],"yoast_head":"\nMultiplexing Strategy with NEBNext Oligos\/Adaptors - New England Biolabs GmbH<\/title>\n<meta name=\"robots\" content=\"noindex, follow\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Multiplexing Strategy with NEBNext Oligos\/Adaptors - New England Biolabs GmbH\" \/>\n<meta property=\"og:description\" content=\"[vc_row][vc_column][vc_basic_grid post_type=”post” max_items=”10″ element_width=”12″ gap=”15″ item=”4123″ grid_id=”vc_gid:1705417750123-0625a967-bd80-6″ taxonomies=”128″][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]Find the best Oligo Strategy for your experiment! [\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_row_inner][vc_column_inner][vc_column_text] NEBNext Multiplex Oligos Selection Chart Single Index Dual Index Unique Dual Index Unique Dual Index UMIs NEB Products NEB #E7335, NEB #E7500, NEB #E7710, NEB #E7730, NEB #E6609 NEB #E7600, NEB #E7780 NEB #E6440 (Set\u00a01) NEB #E6442 (Set\u00a02) NEB... 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