{"id":27171,"date":"2021-03-02T07:56:18","date_gmt":"2021-03-02T06:56:18","guid":{"rendered":"https:\/\/www.neb-online.de\/next-generation-sequencing\/nebnext-artic-kits\/"},"modified":"2025-04-22T11:06:05","modified_gmt":"2025-04-22T09:06:05","slug":"nebnext-artic-kits","status":"publish","type":"page","link":"https:\/\/www.neb-online.de\/en\/next-generation-sequencing\/nebnext-artic-kits\/","title":{"rendered":"NEBNext ARTIC Kits for SARS-CoV-2 Sequencing"},"content":{"rendered":"

[vc_row][vc_column width=”2\/3″][vc_column_text]The NEBNext ARTIC kits meet the increasing need for reliable, accurate and fast methods for sequencing SARSCoV-2, with Oxford Nanopore Technologies or Illumina sequencing. The kits use the ARTIC multiplexed ampliconbased whole-viral-genome sequencing approach and were developed in collaboration with the ARTIC Network<\/a><\/span> (artic. network). The kits include balanced V3 primer pools for improved uniformity of genome coverage and are effective with 10-10,000 SARS-CoV-2 genome copies. The reagents and protocols for RT-PCR and library prep are all optimized specifically for the SARS-CoV-2 ARTIC workflows.<\/p>\n

Improved sequence coverage for current virus variants and express protocols.<\/strong><\/span><\/p>\n

Starting September 2021, two options for primers are now available, in the NEBNext ARTIC SARS-CoV-2 FS Library Prep Kit (Illumina) (NEB #E7658<\/a>), NEBNext ARTIC SARS-CoV-2 Companion Kit (Oxford Nanopore Technologies) (NEB #E7660<\/a>) and NEBNext ARTIC SARS-CoV-2 RT-PCR Module (NEB #E7626<\/a>):<\/p>\n

As of mid-February 2022, you will receive both the “ARTIC V3” primer pool and the VarSkip Short v2 primers (VSSv2) for free with NEBNext ARTIC reagents (not applicable to #E7650). Use the VSSv2 primers to achieve excellent coverage of circulating SARS-CoV-2 variants, including the Omicron and Delta variants. For robust coverage of the latest variants, we recommend using a spike-in primer pool in addition to the VSSv2 primers (see figure below). This pool is available separately on request.<\/p>\n

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  1. Information on primer overlaps with Omicron and Delta variants.<\/a><\/li>\n<\/ol>\n

    [\/vc_column_text][vc_column_text css=”.vc_custom_1671021864101{margin-bottom: 0px !important;border-bottom-width: 0px !important;padding-bottom: 10px !important;}”]Sequence coverage of SARS-CoV-2 genomes with VarSkip Short v2 plus spike-in primers<\/strong>[\/vc_column_text][vc_single_image image=”44221″ img_size=”full” css=”.vc_custom_1671021888069{margin-top: 0px !important;margin-bottom: 0px !important;border-top-width: 0px !important;border-bottom-width: 0px !important;padding-top: 0px !important;padding-bottom: 10px !important;}”][vc_column_text css=”.vc_custom_1671021935880{margin-top: 0px !important;border-top-width: 0px !important;padding-top: 0px !important;}”]Consensus genomes known to be generated using <\/i>VarSkip<\/i> Short 2 (+ supplementary primers) (green) are compared with other genomes (yellow) submitted to <\/i>Genbank<\/i> between 2022-08-01 and 2022-09-24. Genomes were aligned to the NC_045512.2 reference (minimap2 -x map-<\/i>ont<\/i> -r 20000 –score-N=0, v.2.24). Ns were tabulated using <\/i>Qualimap<\/i> (v2.2.2).<\/i><\/span>[\/vc_column_text][vc_separator][vc_column_text]The two kits compatible with Illumina sequencing generate library inserts of ~150 bp (for 2 x 75 sequencing) or ~400 bp (for 2 x 250 sequencing), and include a novel DNA polymerase formulation that eliminates the need to normalize amplicon concentrations prior to library preparation.[\/vc_column_text][\/vc_column][vc_column width=”1\/3″]

    \n\t\t\t\tAdvantages:<\/span>\n\t\t\t\t\t