{"id":28447,"date":"2021-07-21T09:49:02","date_gmt":"2021-07-21T07:49:02","guid":{"rendered":"https:\/\/www.neb-online.de\/next-generation-sequencing\/das-nebnext-immune-sequencing-kit\/"},"modified":"2023-12-19T13:47:28","modified_gmt":"2023-12-19T12:47:28","slug":"the-nebnext-immune-sequencing-kit","status":"publish","type":"page","link":"https:\/\/www.neb-online.de\/en\/next-generation-sequencing\/the-nebnext-immune-sequencing-kit\/","title":{"rendered":"NEBNext Immune-Sequencing Kit"},"content":{"rendered":"

[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_column_text]<\/p>\n

Generate full-length immune gene repertoires of B and T cells from human or mouse cells<\/span><\/h2>\n

[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner][vc_column_inner width=”1\/2″][vc_column_text]With the NEBNext Immune Sequencing Kits (available for mouse<\/a><\/span> and human<\/a><\/span>), sequence the full-length immune gene repertoires of B cells and T cells. Profile somatic mutations across all relevant contexts (e.g., V, D, and J segments and isotypes IgM, IgD, IgG, IgA, and IgE) with improved sequence accuracy. Characterize BCR light, BCR heavy, TCR\u03b1, TCR\u03b2, TCR\u03b3 and TCR\u03b4 chains.<\/p>\n

This kit includes UMIs for source-molecule identification. Additionally, analyze data using a bioinformatic workflow based on the open-source pRESTO toolkit (tutorial).<\/span><\/a>[\/vc_column_text][vc_column_text css=”.vc_custom_1626854070840{margin-bottom: 0px !important;border-bottom-width: 0px !important;padding-bottom: 10px !important;}”]For bioinformatic data analysis, the open source workflow pRESTO (available via Galaxy) is available for free!<\/strong>[\/vc_column_text]Learn more!<\/a>[\/vc_column_inner][vc_column_inner width=”1\/2″][vc_single_image image=”28429″ img_size=”large” css=”.vc_custom_1626791976889{margin-bottom: 0px !important;border-bottom-width: 0px !important;padding-bottom: 10px !important;}”][vc_column_text css=”.vc_custom_1626854002697{margin-bottom: 0px !important;border-bottom-width: 0px !important;padding-bottom: 0px !important;}”]The NEBNext Immune-Seq workflow is just that simple.<\/span><\/em>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner bwd_tbc4vd_show_weekday=””][vc_column_inner][vc_separator css=”.vc_custom_1626854223476{margin-top: 30px !important;}”][\/vc_column_inner][\/vc_row_inner][vc_row_inner bwd_tbc4vd_show_weekday=”” css=”.vc_custom_1626790211806{background-color: #fef2e9 !important;}”][vc_column_inner width=”1\/6″][vc_single_image image=”28435″ img_size=”medium” css=”.vc_custom_1626790169636{margin-top: 10px !important;}”][\/vc_column_inner][vc_column_inner width=”5\/6″][vc_column_text css=”.vc_custom_1626875797020{margin-bottom: 0px !important;border-bottom-width: 0px !important;padding-bottom: 10px !important;}”]Article | Published:

SARS-CoV-2 mRNA vaccines induce persistent human germinal centre responses<\/span><\/strong><\/h2>\n

Jackson S. Turner, Ali H. Ellebedy, et.al.
\nNature<\/i> (2021<\/span>)[\/vc_column_text]Read the Nature-Publication!<\/a>[\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1626853714501{margin-top: 50px !important;}”][\/vc_column][\/vc_row][vc_row bwd_show_when_type=”before” bwd_show_when_date=”12\/31\/2023 23:00″ bwd_tbc4vd_show_weekday=””][vc_column][vc_column_text css=”.vc_custom_1667573282770{margin-bottom: 0px !important;border-bottom-width: 0px !important;padding-bottom: 10px !important;}”]<\/p>\n

Product table<\/strong><\/h3>\n\n\n\n\n\n\n\n
PRODUCT<\/th>\nProd.Nr.:<\/th>\nSIZE<\/th>\nPRICE<\/th>\n<\/tr>\n
NEBNext Immune Sequencing Kit (Mouse)<\/td>\nE6330S<\/td>\n24 rxns<\/td>\n1.790 \u20ac<\/strong><\/td>\n\"NEB<\/a><\/td>\n\"NEB<\/a><\/td>\n<\/tr>\n
NEBNext Immune Sequencing Kit (Mouse)<\/td>\nE6330L<\/td>\n96 rxns<\/td>\n6.138 \u20ac<\/strong><\/td>\n\"NEB<\/a><\/td>\n\"NEB<\/a><\/td>\n<\/tr>\n
NEBNext Immune Sequencing Kit (Human)<\/td>\nE6320S<\/td>\n24 rxns<\/td>\n1.790 \u20ac<\/strong><\/td>\n\"NEB<\/a><\/td>\n\"NEB<\/a><\/td>\n<\/tr>\n
NEBNext Immune Sequencing Kit (Human)<\/td>\nE6320L<\/td>\n96 rxns<\/td>\n6.138 \u20ac<\/strong><\/td>\n\"NEB<\/a><\/td>\n\"NEB<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

As of: 01.01.2023<\/i><\/span>[\/vc_column_text][\/vc_column][\/vc_row][vc_row bwd_show_when_type=”after” bwd_show_when_date=”12\/31\/2023 23:00″ bwd_tbc4vd_show_weekday=””][vc_column][vc_column_text css=”.vc_custom_1702990045542{margin-bottom: 0px !important;border-bottom-width: 0px !important;padding-bottom: 10px !important;}”]<\/p>\n

Product table<\/strong><\/h3>\n\n\n\n\n\n\n\n
PRODUCT<\/th>\nProd.Nr.:<\/th>\nSIZE<\/th>\nINFO<\/th>\n<\/tr>\n
NEBNext Immune Sequencing Kit (Mouse)<\/td>\nE6330S<\/td>\n24 rxns<\/td>\n\"NEB<\/a><\/td>\n\"NEB<\/a><\/td>\n<\/tr>\n
NEBNext Immune Sequencing Kit (Mouse)<\/td>\nE6330L<\/td>\n96 rxns<\/td>\n\"NEB<\/a><\/td>\n\"NEB<\/a><\/td>\n<\/tr>\n
NEBNext Immune Sequencing Kit (Human)<\/td>\nE6320S<\/td>\n24 rxns<\/td>\n\"NEB<\/a><\/td>\n\"NEB<\/a><\/td>\n<\/tr>\n
NEBNext Immune Sequencing Kit (Human)<\/td>\nE6320L<\/td>\n96 rxns<\/td>\n\"NEB<\/a><\/td>\n\"NEB<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

As of: 01.01.2024<\/i><\/span>[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"

[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_column_text] Generate full-length immune gene repertoires of B and T cells from human or mouse cells [\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner][vc_column_inner width=”1\/2″][vc_column_text]With the NEBNext Immune Sequencing Kits (available for mouse and human), sequence the full-length immune gene repertoires of B cells and T cells. Profile somatic mutations across all relevant contexts (e.g., V, D, and J segments and isotypes… Read more »<\/a><\/p>\n","protected":false},"author":18,"featured_media":25770,"parent":2170,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-sidebar.php","meta":{"footnotes":""},"class_list":["post-28447","page","type-page","status-publish","has-post-thumbnail","hentry"],"yoast_head":"\nNEBNext Immune-Sequencing Kit - New England Biolabs GmbH<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.neb-online.de\/next-generation-sequencing\/das-nebnext-immune-sequencing-kit\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"NEBNext Immune-Sequencing Kit - New England Biolabs GmbH\" \/>\n<meta property=\"og:description\" content=\"[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_column_text] Generate full-length immune gene repertoires of B and T cells from human or mouse cells [\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner][vc_column_inner width=”1\/2″][vc_column_text]With the NEBNext Immune Sequencing Kits (available for mouse and human), sequence the full-length immune gene repertoires of B cells and T cells. 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