{"id":3382,"date":"2015-05-21T10:53:59","date_gmt":"2015-05-21T10:53:59","guid":{"rendered":"http:\/\/www.neb-online.de\/?page_id=3382"},"modified":"2021-09-09T09:25:19","modified_gmt":"2021-09-09T07:25:19","slug":"technical-resources-neb","status":"publish","type":"page","link":"https:\/\/www.neb-online.de\/en\/technical-resources-neb\/","title":{"rendered":"Technical Resources NEB"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column width=&#8221;1\/2&#8243;][vc_toggle title=&#8221;Cloning &amp; Synthetic Biology&#8221; style=&#8221;round&#8221; color=&#8221;sandy_brown&#8221; size=&#8221;lg&#8221; el_id=&#8221;1488451520888-17f3b7b7-90fc&#8221;]<\/p>\n<h3>Tools<\/h3>\n<ul>\n<li><a href=\"http:\/\/nebcloner.neb.com\/#%21\/\" target=\"_blank\" rel=\"noopener noreferrer\">NEBCloner<\/a><\/li>\n<li><a href=\"http:\/\/nebuilder.neb.com\" target=\"_blank\" rel=\"noopener noreferrer\">NEBuilder<\/a><\/li>\n<li><a href=\"https:\/\/www.neb.com\/en\/external-links\/neb-golden-gate-assembly-tool\" target=\"_blank\" rel=\"noopener noreferrer\">NEB Golden Gate Assembly Tool<\/a><\/li>\n<li><a href=\"https:\/\/nebcloner.neb.com\/#!\/redigest\" target=\"_blank\" rel=\"noopener noreferrer\">Double Digest Finder<\/a><\/li>\n<li><a href=\"http:\/\/nc2.neb.com\/NEBcutter2\/\" target=\"_blank\" rel=\"noopener noreferrer\">NEB Cutter<\/a><\/li>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/interactive-tools\/dna-sequences-and-maps-tool\" target=\"_blank\" rel=\"noopener noreferrer\">DNA Sequences and Maps Tool<\/a><\/li>\n<li><a href=\"https:\/\/enzymefinder.neb.com\/\" target=\"_blank\" rel=\"noopener noreferrer\">Enzyme Finder<\/a><\/li>\n<\/ul>\n<h3>Selection Tools<\/h3>\n<ul>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/selection-charts\/synthetic-biology-dna-assembly-selection-chart\" target=\"_blank\" rel=\"noopener noreferrer\">Synthetic Biology\/DNA Assembly Selection Chart<\/a><\/li>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/selection-charts\/dna-ligase-selection-chart\" target=\"_blank\" rel=\"noopener noreferrer\">DNA Ligase Selection Chart<\/a><\/li>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/selection-charts\/isoschizomers\" target=\"_blank\" rel=\"noopener noreferrer\">Isoschizomere der Restriktionsenzyme<\/a><\/li>\n<\/ul>\n<h3>Troubleshooting Guides<\/h3>\n<ul>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/troubleshooting-guides\/troubleshooting-guide-for-cloning\" target=\"_blank\" rel=\"noopener noreferrer\">Troubleshooting Guide for Cloning<\/a><\/li>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/troubleshooting-guides\/restriction-enzyme-troubleshooting-guide\" target=\"_blank\" rel=\"noopener noreferrer\">Restriction Enzyme Troubleshooting Guide<\/a><\/li>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/troubleshooting-guides\/troubleshooting-guide-for-ligases\" target=\"_blank\" rel=\"noopener noreferrer\">Troubleshooting Guide for Ligases<\/a><\/li>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/troubleshooting-guides\/troubleshooting-guide-for-end-modification\" target=\"_blank\" rel=\"noopener noreferrer\">Troubleshooting Guide for End Modification<\/a><\/li>\n<\/ul>\n<h3>Hinweise und Tipps<\/h3>\n<ul>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/usage-guidelines\/restriction-enzyme-tips\" target=\"_blank\" rel=\"noopener noreferrer\">Restriction Enzyme Tips<\/a><\/li>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/usage-guidelines\/optimizing-restriction-endonuclease-reactions\" target=\"_blank\" rel=\"noopener noreferrer\">Optimizing Restriction Endonuclease Reactions<\/a><\/li>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/usage-guidelines\/restriction-enzymes-requiring-multi-sites-for-efficient-cleavage\">Restriction enzymes requiring multi-sites for efficient cleavage<\/a><\/li>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/usage-guidelines\/nebuffer-performance-chart-with-restriction-enzymes\" target=\"_blank\" rel=\"noopener noreferrer\">NEBuffer Activity\/Performance Chart with Restriction Enzymes<\/a><\/li>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/usage-guidelines\/activity-of-restriction-enzymes-in-a-q5-taq-or-phusion-pcr-mix\" target=\"_blank\" rel=\"noopener noreferrer\">Activity of Restriction Enzymes in a Q5\u00ae, Taq or Phusion PCR Mix<\/a><\/li>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/selection-charts\/cloning-plasmids-and-dnas\" target=\"_blank\" rel=\"noopener noreferrer\">Cloning Plasmids and DNAs Overview<\/a><\/li>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/selection-charts\/compatible-cohesive-ends-and-generation-of-new-restriction-sites\" target=\"_blank\" rel=\"noopener noreferrer\">Compatible Cohesive Ends and Generation of New Restriction Sites<\/a><\/li>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/selection-charts\/dam-dcm-and-cpg-methylation\" target=\"_blank\" rel=\"noopener noreferrer\">Dam-Dcm and CpG Methylation<\/a><\/li>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/selection-charts\/frequencies-of-restriction-sites\" target=\"_blank\" rel=\"noopener noreferrer\">Frequencies of Restriction Sites<\/a><\/li>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/selection-charts\/recleavable-blunt-ends\" target=\"_blank\" rel=\"noopener noreferrer\">Recleavable Blunt Ends<\/a><\/li>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/selection-charts\/recleavable-filled-5-overhangs\" target=\"_blank\" rel=\"noopener noreferrer\">Recleavable Filled-in 5\u2032 Overhangs<\/a><\/li>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/selection-charts\/cleavage-of-supercoiled-dna\" target=\"_blank\" rel=\"noopener noreferrer\">Cleavage of Supercoiled DNA<\/a><\/li>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/usage-guidelines\/activity-at-37c-for-restriction-enzymes-with-alternate-incubation-temperatures\" target=\"_blank\" rel=\"noopener noreferrer\">Activity at 37\u00b0C for Restriction Enzymes with Alternate Incubation Temperatures<\/a><\/li>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/usage-guidelines\/digestion-of-agarose-embedded-dna-info-for-specific-enzymes\" target=\"_blank\" rel=\"noopener noreferrer\">Digestion of Agarose-Embedded DNA: Info for Specific Enzymes<\/a><\/li>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/usage-guidelines\/restriction-endonucleases-survival-in-a-reaction\" target=\"_blank\" rel=\"noopener noreferrer\">Restriction Endonucleases \u2013 Survival in a Reaction<\/a><\/li>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/usage-guidelines\/cloning-guide\" target=\"_blank\" rel=\"noopener noreferrer\">Traditional Cloning Quick Guide<\/a><\/li>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/usage-guidelines\/tips-for-maximizing-ligation-efficiencies\" target=\"_blank\" rel=\"noopener noreferrer\">Tips for Maximizing Ligation Efficiencies<\/a><\/li>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/usage-guidelines\/end-modification-tips\" target=\"_blank\" rel=\"noopener noreferrer\">End-Modification Tips<\/a><\/li>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/usage-guidelines\/cleavage-close-to-the-end-of-dna-fragments\" target=\"_blank\" rel=\"noopener noreferrer\">Cleavage Close to the End of DNA Fragments<\/a><\/li>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/selection-charts\/effect-of-various-temperatures-on-nicking-endonucleases\">Effect of various temperatures on Nicking Endonucleases<\/a><\/li>\n<\/ul>\n<ul>\n<li><span style=\"font-size: 1.125em;\">Application Notes<\/span><\/li>\n<\/ul>\n<ul>\n<li><a href=\"https:\/\/www.neb.com\/en\/-\/media\/nebus\/files\/application-notes\/appnote_improved_methods_for_sdm_using_gibson-assembly_master_mix.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Improved methods for site-directed mutagenesis using Gibson Assembly\u00ae Master Mix<\/a><\/li>\n<li><a href=\"https:\/\/www.neb.com\/en\/-\/media\/nebus\/files\/application-notes\/appnote_joining_of_difficult_to_ligate_dsdna_fragments_with_blunt_ta_ligase_master_mix.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Joining of Difficult to Ligate dsDNA Fragments with Blunt\/TA Ligase Master Mix<\/a><\/li>\n<\/ul>\n<h3>Video Tutorials<\/h3>\n<p><iframe loading=\"lazy\" src=\"https:\/\/www.youtube-nocookie.com\/embed\/LfaxJfTSyzM?rel=0\" width=\"300\" height=\"169\" frameborder=\"0\" allowfullscreen=\"allowfullscreen\"><\/iframe> <iframe loading=\"lazy\" src=\"https:\/\/www.youtube-nocookie.com\/embed\/DCxl1GlIkzI?rel=0\" width=\"300\" height=\"169\" frameborder=\"0\" allowfullscreen=\"allowfullscreen\"><\/iframe><br \/>\n<iframe loading=\"lazy\" src=\"https:\/\/www.youtube-nocookie.com\/embed\/LwOYvWTm7kE?rel=0\" width=\"300\" height=\"169\" frameborder=\"0\" allowfullscreen=\"allowfullscreen\"><\/iframe> <iframe loading=\"lazy\" src=\"https:\/\/www.youtube-nocookie.com\/embed\/8_-t5xtJ3y8?rel=0\" width=\"300\" height=\"169\" frameborder=\"0\" allowfullscreen=\"allowfullscreen\"><\/iframe><br \/>\n<iframe loading=\"lazy\" src=\"https:\/\/www.youtube-nocookie.com\/embed\/b9Lm2TUDN2M?rel=0\" width=\"300\" height=\"169\" frameborder=\"0\" allowfullscreen=\"allowfullscreen\"><\/iframe> <iframe loading=\"lazy\" src=\"https:\/\/www.youtube-nocookie.com\/embed\/TaNRbvq9dzY?rel=0\" width=\"300\" height=\"169\" frameborder=\"0\" allowfullscreen=\"allowfullscreen\"><\/iframe>[\/vc_toggle][vc_toggle title=&#8221;RNA Biology&#8221; style=&#8221;round&#8221; color=&#8221;sandy_brown&#8221; size=&#8221;lg&#8221; el_id=&#8221;1488456542829-540867d8-6d9d&#8221;]<\/p>\n<h3>Hinweise und Tipps<\/h3>\n<ul>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/selection-charts\/rna-polymerase-selection-chart\" target=\"_blank\" rel=\"noopener noreferrer\">RNA Polymerase Selection Chart<\/a><\/li>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/selection-charts\/rna-ligase-selection-chart\" target=\"_blank\" rel=\"noopener noreferrer\">RNA Ligase Selection Chart<\/a><\/li>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/selection-charts\/cdna-synthesis-selection\" target=\"_blank\" rel=\"noopener noreferrer\">cDNA Synthesis Selection Chart<\/a><\/li>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/selection-charts\/recommended-hiscribe-rna-synthesis-kits-by-application\" target=\"_blank\" rel=\"noopener noreferrer\">Recommended HiScribe RNA Synthesis Kits by Application<\/a><\/li>\n<\/ul>\n<h3>Guides<\/h3>\n<ul>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/usage-guidelines\/avoiding-ribonuclease-contamination\" target=\"_blank\" rel=\"noopener noreferrer\">Avoiding Ribonuclease Contamination<\/a><\/li>\n<\/ul>\n<h3>Video Tutorials<\/h3>\n<p><iframe loading=\"lazy\" src=\"https:\/\/www.youtube-nocookie.com\/embed\/jgPUOHX0k3c?rel=0\" width=\"300\" height=\"169\" frameborder=\"0\" allowfullscreen=\"allowfullscreen\"><\/iframe>[\/vc_toggle][vc_toggle title=&#8221;Glycobiology&#8221; style=&#8221;round&#8221; color=&#8221;sandy_brown&#8221; size=&#8221;lg&#8221; el_id=&#8221;1488456653191-2207a997-2a7e&#8221;]<\/p>\n<h3>Tools<\/h3>\n<ul>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/usage-guidelines\/glycobiology-unit-conversion-chart\" target=\"_blank\" rel=\"noopener noreferrer\">Glycobiology Unit Conversion Chart<\/a><\/li>\n<\/ul>\n<h3>Hinweise und Tipps<\/h3>\n<ul>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/usage-guidelines\/detailed-characterization-of-several-glycosidase-enzymes\" target=\"_blank\" rel=\"noopener noreferrer\">Detailed Characterization of Several Glycosidase Enzymes<\/a><\/li>\n<\/ul>\n<h3>FAQs<\/h3>\n<ul>\n<li><a href=\"https:\/\/www.neb.com\/en\/faqs\/2015\/04\/01\/why-have-the-neb-glycosidase-enzymes-changed-reaction-buffers-what-are-the-new-reaction-buffers-a\" target=\"_blank\" rel=\"noopener noreferrer\">GlycoBuffer System<\/a><\/li>\n<\/ul>\n<h3>Application Notes<\/h3>\n<ul>\n<li><a href=\"https:\/\/www.neb.com\/en\/protocols\/2012\/06\/04\/1-3-6-galactosidase-application-note-p0731\" target=\"_blank\" rel=\"noopener noreferrer\">\u03b11-3,6 Galactosidase Application Note<\/a><\/li>\n<li><a href=\"https:\/\/www.neb.com\/en\/-\/media\/nebus\/files\/application-notes\/enzymatic-removal-of-n--and-o-glycans-using-pngase-f-or-the-protein-deglycosylation-mix.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Enzymatic removal of N- and O-glycans using PNGase F or the Protein Deglycosylation Mix P0704<\/a><\/li>\n<li><a href=\"https:\/\/www.neb.com\/en\/protocols\/0001\/01\/01\/o-glycosidase-application-note-1-p0733\" target=\"_blank\" rel=\"noopener noreferrer\">O-Glycosidase Application Note 1<\/a><\/li>\n<\/ul>\n<h3>Video Tutorials<\/h3>\n<p><iframe loading=\"lazy\" src=\"https:\/\/www.youtube-nocookie.com\/embed\/y570P1ynDp0?rel=0\" width=\"300\" height=\"169\" frameborder=\"0\" allowfullscreen=\"allowfullscreen\"><\/iframe> <iframe loading=\"lazy\" src=\"https:\/\/www.youtube-nocookie.com\/embed\/9wcxfVZHGNg?rel=0\" width=\"300\" height=\"169\" frameborder=\"0\" allowfullscreen=\"allowfullscreen\"><\/iframe>[\/vc_toggle][vc_toggle title=&#8221;Epigenetics&#8221; style=&#8221;round&#8221; color=&#8221;sandy_brown&#8221; size=&#8221;lg&#8221; el_id=&#8221;1488456833131-dd5a6e58-225e&#8221;]<\/p>\n<h3>Troubleshooting Guides<\/h3>\n<ul>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/troubleshooting-guides\/epimark-methylated-dna-enrichment-kit-troubleshooting-guide\" target=\"_blank\" rel=\"noopener noreferrer\">EpiMark\u00ae Methylated DNA Enrichment Kit Troubleshooting Guide<\/a><\/li>\n<\/ul>\n<h3>Application Notes<\/h3>\n<ul>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/app-notes\/analysis-of-5-methylcytosine-and-5-hydroxymethylcytosine-levels-epimark-analysis-e3317\" target=\"_blank\" rel=\"noopener noreferrer\">Analysis of 5-methylcytosine and 5-hydroxymethylcytosine Levels in Human Brain and Liver DNA Samples using the EpiMark\u00ae 5-hmC and 5-mC Analysis Kit<\/a><\/li>\n<\/ul>\n<h3>Video Tutorials<\/h3>\n<p><iframe loading=\"lazy\" src=\"https:\/\/www.youtube-nocookie.com\/embed\/nygyUMODV7Y?rel=0\" width=\"300\" height=\"169\" frameborder=\"0\" allowfullscreen=\"allowfullscreen\"><\/iframe> <iframe loading=\"lazy\" src=\"https:\/\/www.youtube-nocookie.com\/embed\/jYy3nn26QeE?rel=0\" width=\"300\" height=\"169\" frameborder=\"0\" allowfullscreen=\"allowfullscreen\"><\/iframe><br \/>\n<iframe loading=\"lazy\" src=\"https:\/\/www.youtube-nocookie.com\/embed\/w-NWhaB185s?rel=0\" width=\"300\" height=\"169\" frameborder=\"0\" allowfullscreen=\"allowfullscreen\"><\/iframe>[\/vc_toggle][vc_toggle title=&#8221;DNA \/ RNA Ladders&#8221; style=&#8221;round&#8221; color=&#8221;sandy_brown&#8221; size=&#8221;lg&#8221; el_id=&#8221;1488456728240-cde2ba6f-a784&#8243;]<\/p>\n<h3>Selection Tools<\/h3>\n<ul>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/selection-charts\/dna-markers-and-ladders-selection-chart\" target=\"_blank\" rel=\"noopener noreferrer\">DNA Markers &amp; Ladders Selection Chart<\/a><\/li>\n<\/ul>\n<p>[\/vc_toggle][\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_toggle title=&#8221;PCR DNA Amplification&#8221; style=&#8221;round&#8221; color=&#8221;sandy_brown&#8221; size=&#8221;lg&#8221; el_id=&#8221;1488456325239-2b546c38-1e52&#8243;]<\/p>\n<h3>Tools<\/h3>\n<ul>\n<li><a href=\"http:\/\/tmcalculator.neb.com\/#%21\/\" target=\"_blank\" rel=\"noopener noreferrer\">NEB Tm Calculator<\/a><\/li>\n<li><a href=\"http:\/\/nebasechanger.neb.com\" target=\"_blank\" rel=\"noopener noreferrer\">NEBaseChanger<\/a><\/li>\n<\/ul>\n<h3>Selection Tools<\/h3>\n<ul>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/selection-charts\/dna-polymerase-selection-chart\" target=\"_blank\" rel=\"noopener noreferrer\">DNA Polymerase Selection Chart<\/a><\/li>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/interactive-tools\/pcr-selection-tool\" target=\"_blank\" rel=\"noopener noreferrer\">PCR Selection Tool<\/a><\/li>\n<\/ul>\n<h3>Troubleshooting Guides<\/h3>\n<ul>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/troubleshooting-guides\/pcr-troubleshooting-guide\" target=\"_blank\" rel=\"noopener noreferrer\">PCR Troubleshooting Guide<\/a><\/li>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/troubleshooting-guides\/taq-pcr-kit-troubleshooting-guide\" target=\"_blank\" rel=\"noopener noreferrer\"><em>Taq <\/em>PCR Kit Troubleshooting Guide<\/a><\/li>\n<\/ul>\n<h3>Hinweise und Tipps<\/h3>\n<ul>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/usage-guidelines\/guidelines-for-pcr-optimization-with-taq-dna-polymerase\" target=\"_blank\" rel=\"noopener noreferrer\">Guidelines for PCR Optimization with <em>Taq<\/em> DNA Polymerase<\/a><\/li>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/usage-guidelines\/guidelines-for-pcr-optimization-with-onetaq-and-onetaq-hot-start-dna-polymerases\" target=\"_blank\" rel=\"noopener noreferrer\">Guidelines for PCR Optimization with OneTaq\u00ae and OneTaq\u00ae Hot Start DNA Polymerases<\/a><\/li>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/usage-guidelines\/guidelines-for-pcr-optimization-with-thermophilic-dna-polymerases\" target=\"_blank\" rel=\"noopener noreferrer\">Guidelines for PCR Optimization with Thermophilic DNA Polymerases<\/a><\/li>\n<\/ul>\n<h3>FAQs<\/h3>\n<ul>\n<li><a href=\"https:\/\/www.neb.com\/en\/products\/pcr-polymerases-and-amplification-technologies\/%7E\/media\/26059D85ADCD473DB16C5500AD69EE60.ashx\" target=\"_blank\" rel=\"noopener noreferrer\">Polymerases and Amplification FAQs<\/a><\/li>\n<\/ul>\n<h3>Application Notes<\/h3>\n<ul>\n<li><a href=\"https:\/\/www.neb.com\/en\/-\/media\/nebus\/files\/application-notes\/appnote_colony_pcr.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Robust Colony PCR from Multiple E. coli Strains using OneTaq\u00ae Quick-Load\u00ae Master Mixes<\/a><\/li>\n<\/ul>\n<h3>Video Tutorials<\/h3>\n<p><iframe loading=\"lazy\" src=\"https:\/\/www.youtube-nocookie.com\/embed\/QK5HbmdSfn0?rel=0\" width=\"300\" height=\"169\" frameborder=\"0\" allowfullscreen=\"allowfullscreen\"><\/iframe> <iframe loading=\"lazy\" src=\"https:\/\/www.youtube-nocookie.com\/embed\/Rpw1Tab5BOs?rel=0\" width=\"300\" height=\"169\" frameborder=\"0\" allowfullscreen=\"allowfullscreen\"><\/iframe>[\/vc_toggle][vc_toggle title=&#8221;Next Generation Sequencing&#8221; style=&#8221;round&#8221; color=&#8221;sandy_brown&#8221; size=&#8221;lg&#8221; el_id=&#8221;1488456420649-6dfa5259-7260&#8243;]<\/p>\n<h3>Tools<\/h3>\n<ul>\n<li><a href=\"http:\/\/nebiocalculator.neb.com\/#%21\/qPCR\" target=\"_blank\" rel=\"noopener noreferrer\">NEBioCalculator \/ qPCR<\/a><\/li>\n<\/ul>\n<h3>Selection Tools<\/h3>\n<ul>\n<li><a href=\"https:\/\/www.neb.com\/en\/%7E\/media\/NebUs\/Files\/NEBNext%20Chart%20pdfs\/Illumina_SelectionChart.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Illumina Selection Chart<\/a><\/li>\n<li><a href=\"https:\/\/www.neb.com\/en\/%7E\/media\/NebUs\/Files\/NEBNext%20Chart%20pdfs\/IonTorrent_SelectionChart.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Ion Torrent\u2122 Selection Chart<\/a><\/li>\n<\/ul>\n<h3>Hinweise und Tipps<\/h3>\n<ul>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/usage-guidelines\/getting-started-with-rna-seq\" target=\"_blank\" rel=\"noopener noreferrer\">Getting Started with RNA-Seq<\/a><\/li>\n<\/ul>\n<h3>Application Notes<\/h3>\n<ul>\n<li><a href=\"https:\/\/www.neb.com\/en\/%7E\/media\/Catalog\/All-Products\/23EF8BC783C749D9B664D90C8C72FA73\/Application%20Notes\/Low%20input%20ChIPSeq%20using%20the%20NEBNext%20Ultra%20Library%20Prep%20Kit%20for%20Illumina%20E7370.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Low input ChIPSeq using the NEBNext Ultra Library Prep Kit for Illumina E7370<\/a><\/li>\n<li><a href=\"https:\/\/www.neb.com\/en\/%7E\/media\/catalog\/all-products\/7d0645075eaa4a07843194c69eb391a7\/application%20notes\/automated%20size%20selection%20of%20nebnext%20small%20rna%20libraries%20with%20the%20sage%20pippin%20prep%20e7330.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Automated size selection of NEBNext Small RNA libraries with the Sage Pippin Prep<\/a><\/li>\n<li><a href=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/05\/NEB_NEBNext_ULTRA_DNA_Automation.pdf\">Automated Library Preparation using NEBNext Ultra DNA Library<\/a><br \/>\n<a href=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/05\/NEB_NEBNext_ULTRA_DNA_Automation.pdf\">Prep Kit for Illumina on the HAMILTON STAR Line<\/a><\/li>\n<\/ul>\n<p><iframe loading=\"lazy\" src=\"https:\/\/www.youtube-nocookie.com\/embed\/2ppko_PyrjM?rel=0\" width=\"300\" height=\"169\" frameborder=\"0\" allowfullscreen=\"allowfullscreen\"><\/iframe> <iframe loading=\"lazy\" src=\"https:\/\/www.youtube-nocookie.com\/embed\/W5gOlxBB-7k?rel=0\" width=\"300\" height=\"169\" frameborder=\"0\" allowfullscreen=\"allowfullscreen\"><\/iframe><br \/>\n<iframe loading=\"lazy\" src=\"https:\/\/www.youtube-nocookie.com\/embed\/jMd1Xb7GaoU?rel=0\" width=\"300\" height=\"169\" frameborder=\"0\" allowfullscreen=\"allowfullscreen\"><\/iframe> <iframe loading=\"lazy\" src=\"https:\/\/www.youtube-nocookie.com\/embed\/l0BysUXmC2o?rel=0\" width=\"300\" height=\"169\" frameborder=\"0\" allowfullscreen=\"allowfullscreen\"><\/iframe>[\/vc_toggle][vc_toggle title=&#8221;Competent Cells&#8221; style=&#8221;round&#8221; color=&#8221;sandy_brown&#8221; size=&#8221;lg&#8221; el_id=&#8221;1488456608016-39c7a574-06cc&#8221;]<\/p>\n<h3>Selection Tools<\/h3>\n<ul>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/selection-charts\/characteristics-of-select-e-coli-strains\" target=\"_blank\" rel=\"noopener noreferrer\">Characteristics of Select E.coli Strains <\/a><\/li>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/selection-charts\/competent-cell-selection-guide\" target=\"_blank\" rel=\"noopener noreferrer\">Competent Cell Selection Guide<\/a><\/li>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/interactive-tools\/competitor-cross-reference-tool\" target=\"_blank\" rel=\"noopener noreferrer\">Competitor Cross-Reference Tool<\/a><\/li>\n<\/ul>\n<h3>Troubleshooting Guides<\/h3>\n<ul>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/troubleshooting-guides\/troubleshooting-transformation-reactions\" target=\"_blank\" rel=\"noopener noreferrer\">Troubleshooting Transformation Reactions<\/a><\/li>\n<\/ul>\n<h3>Hinweise und Tipps<\/h3>\n<ul>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/usage-guidelines\/genetic-markers\" target=\"_blank\" rel=\"noopener noreferrer\">Genetic Markers<\/a><\/li>\n<li><a href=\"https:\/\/www.neb.com\/en\/protocols\/2012\/06\/21\/making-your-own-electrocompetent-cells\" target=\"_blank\" rel=\"noopener noreferrer\">Making your own electrocompetent cells<\/a><\/li>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/usage-guidelines\/making-unmethylated-dam-dcm-dna\" target=\"_blank\" rel=\"noopener noreferrer\">Making Unmethylated (Dam- Dcm-) DNA<\/a><\/li>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/usage-guidelines\/restriction-of-foreign-dna-by-e-coli-k-12\" target=\"_blank\" rel=\"noopener noreferrer\">Restriction of Foreign DNA by E. coli K-12<\/a><\/li>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/usage-guidelines\/additional-e-coli-strain-genotypes\" target=\"_blank\" rel=\"noopener noreferrer\">Additional E. coli Strain Genotypes<\/a><\/li>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/usage-guidelines\/mcra-mcrbc-and-ecoki-strain-phenotypes\" target=\"_blank\" rel=\"noopener noreferrer\">McrA, McrBC and EcoKI Strain Phenotypes<\/a><\/li>\n<\/ul>\n<h3>Video Tutorials<\/h3>\n<p><iframe loading=\"lazy\" src=\"https:\/\/www.youtube-nocookie.com\/embed\/7Ul9RVYG5CM?rel=0\" width=\"300\" height=\"169\" frameborder=\"0\" allowfullscreen=\"allowfullscreen\"><\/iframe>[\/vc_toggle][vc_toggle title=&#8221;Proteinexpression&#8221; style=&#8221;round&#8221; color=&#8221;sandy_brown&#8221; size=&#8221;lg&#8221; el_id=&#8221;1488456777455-9ac2b9d4-9b46&#8243;]<\/p>\n<h3>Selection Tools<\/h3>\n<ul>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/selection-charts\/protein-expression-and-purification-selection-chart\" target=\"_blank\" rel=\"noopener noreferrer\">Protein Expression and Purification Selection Chart<\/a><\/li>\n<\/ul>\n<h3>FAQs<\/h3>\n<ul>\n<li><a href=\"https:\/\/www.neb.com\/en\/%7E\/media\/NebUs\/Files\/PDF%20FAQ\/PURExpress%20FAQs.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">FAQs for PURExpress<\/a><\/li>\n<\/ul>\n<h3>Application Notes<\/h3>\n<ul>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/app-notes\/use-of-the-purexpress\" target=\"_blank\" rel=\"noopener noreferrer\">Use of the PURExpress\u00ae in vitro Protein Synthesis Kit, Disulfide Bond Enhancer and SHuffle\u00ae Competent E. coli for heterologous in vitro and in vivo cellulase expression.<\/a><\/li>\n<\/ul>\n<h3>Video Tutorials<\/h3>\n<p><iframe loading=\"lazy\" src=\"https:\/\/www.youtube-nocookie.com\/embed\/L5owMAtnVnQ?rel=0\" width=\"300\" height=\"169\" frameborder=\"0\" allowfullscreen=\"allowfullscreen\"><\/iframe>[\/vc_toggle][vc_toggle title=&#8221;Cellular analysis (SNAP-tag)&#8221; style=&#8221;round&#8221; color=&#8221;sandy_brown&#8221; size=&#8221;lg&#8221; el_id=&#8221;1488456942229-cc604e98-40de&#8221;]<\/p>\n<h3>Selection Tools<\/h3>\n<ul>\n<li><a href=\"https:\/\/www.neb.com\/en\/products\/cellular-analysis\/cellular-analysis#tabselect6\" target=\"_blank\" rel=\"noopener noreferrer\">SNAP-tag\u00ae, CLIP-tag\u2122 and ACP\/MCP-tag Substrate Selection Chart<\/a><\/li>\n<\/ul>\n<h3>Troubleshooting Guides<\/h3>\n<ul>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/troubleshooting-guides\/labeling-with-snap-tag-technology-troubleshooting-guide\" target=\"_blank\" rel=\"noopener noreferrer\">Labeling with SNAP-tag\u00ae Technology Troubleshooting Guide<\/a><\/li>\n<\/ul>\n<h3>FAQs<\/h3>\n<ul>\n<li><a href=\"https:\/\/www.neb.com\/en\/products\/cellular-analysis\/%7E\/media\/311B6B8E63B54710940BA4273156E411.ashx\" target=\"_blank\" rel=\"noopener noreferrer\">Cellular Imaging and Analysis FAQs<\/a><\/li>\n<\/ul>\n<h3>Application Notes<\/h3>\n<ul>\n<li><a href=\"https:\/\/www.neb.com\/en\/-\/media\/nebus\/files\/application-notes\/appnote_labeling_of_escherichia_coli_expressed_snap-tag_fusion_proteins.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Labeling of Escherichia coli Expressed SNAP-tag\u00ae Fusion Proteins<\/a><\/li>\n<li><a href=\"https:\/\/www.neb.com\/en\/-\/media\/nebus\/files\/application-notes\/labeling-and-imaging-of-cell-surface-receptors-mediated-by-snaptag-s9143.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Labeling and Imaging of Cell Surface Receptors Mediated by SNAP-tag\u00ae<\/a><\/li>\n<li><a href=\"https:\/\/www.neb.com\/en\/-\/media\/nebus\/files\/application-notes\/appnote_fluorescent_labeling_of_proteins_in_living_cells.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Simultaneous Labeling of Two Proteins in Live Cells<\/a><\/li>\n<li><a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/app-notes\/snapf-based-pulse-labeling-for-analysis-of-protein-turnover-in-living-cells\" target=\"_blank\" rel=\"noopener noreferrer\">SNAPf based pulse labeling for analysis of protein turnover in living cells<\/a><\/li>\n<\/ul>\n<h3>Video Tutorials<\/h3>\n<p><iframe loading=\"lazy\" src=\"https:\/\/www.youtube-nocookie.com\/embed\/NBRk3f6q-_M?rel=0\" width=\"300\" height=\"169\" frameborder=\"0\" allowfullscreen=\"allowfullscreen\"><\/iframe> <iframe loading=\"lazy\" src=\"https:\/\/www.youtube-nocookie.com\/embed\/w3ifXpjuE-M?rel=0\" width=\"300\" height=\"169\" frameborder=\"0\" allowfullscreen=\"allowfullscreen\"><\/iframe>[\/vc_toggle][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row][vc_column width=&#8221;1\/2&#8243;][vc_toggle title=&#8221;Cloning &amp; Synthetic Biology&#8221; style=&#8221;round&#8221; color=&#8221;sandy_brown&#8221; size=&#8221;lg&#8221; el_id=&#8221;1488451520888-17f3b7b7-90fc&#8221;] Tools NEBCloner NEBuilder NEB Golden Gate Assembly Tool Double Digest Finder NEB Cutter DNA Sequences and Maps Tool Enzyme Finder Selection Tools Synthetic Biology\/DNA Assembly Selection Chart DNA Ligase Selection Chart Isoschizomere der Restriktionsenzyme Troubleshooting Guides Troubleshooting Guide for Cloning Restriction Enzyme Troubleshooting Guide Troubleshooting&#8230;  <a class=\"excerpt-read-more\" href=\"https:\/\/www.neb-online.de\/en\/technical-resources-neb\/\" title=\"Read Technical Resources NEB\">Read more &raquo;<\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"open","ping_status":"closed","template":"page-sidebar.php","meta":{"footnotes":""},"class_list":["post-3382","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.1.1 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Technical Resources NEB - New England Biolabs GmbH<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.neb-online.de\/en\/technical-resources-neb\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Technical Resources NEB - New England Biolabs GmbH\" 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Read more &raquo;\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.neb-online.de\/en\/technical-resources-neb\/\" \/>\n<meta property=\"og:site_name\" content=\"New England Biolabs GmbH\" \/>\n<meta property=\"article:modified_time\" content=\"2021-09-09T07:25:19+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2019\/03\/NEB_GmbH_with-inspired-claim-square_4c.png\" \/>\n\t<meta property=\"og:image:width\" content=\"500\" \/>\n\t<meta property=\"og:image:height\" content=\"266\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/png\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"4 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.neb-online.de\/en\/technical-resources-neb\/\",\"url\":\"https:\/\/www.neb-online.de\/en\/technical-resources-neb\/\",\"name\":\"Technical Resources NEB - New England Biolabs GmbH\",\"isPartOf\":{\"@id\":\"https:\/\/www.neb-online.de\/#website\"},\"datePublished\":\"2015-05-21T10:53:59+00:00\",\"dateModified\":\"2021-09-09T07:25:19+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/www.neb-online.de\/en\/technical-resources-neb\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/www.neb-online.de\/en\/technical-resources-neb\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/www.neb-online.de\/en\/technical-resources-neb\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/www.neb-online.de\/en\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Technical Resources NEB\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/www.neb-online.de\/#website\",\"url\":\"https:\/\/www.neb-online.de\/\",\"name\":\"New England Biolabs GmbH\",\"description\":\"Enabling Technologies in the Life Sciences\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/www.neb-online.de\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Technical Resources NEB - New England Biolabs GmbH","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.neb-online.de\/en\/technical-resources-neb\/","og_locale":"en_US","og_type":"article","og_title":"Technical Resources NEB - New England Biolabs GmbH","og_description":"[vc_row][vc_column width=&#8221;1\/2&#8243;][vc_toggle title=&#8221;Cloning &amp; Synthetic Biology&#8221; style=&#8221;round&#8221; color=&#8221;sandy_brown&#8221; size=&#8221;lg&#8221; el_id=&#8221;1488451520888-17f3b7b7-90fc&#8221;] Tools NEBCloner NEBuilder NEB Golden Gate Assembly Tool Double Digest Finder NEB Cutter DNA Sequences and Maps Tool Enzyme Finder Selection Tools Synthetic Biology\/DNA Assembly Selection Chart DNA Ligase Selection Chart Isoschizomere der Restriktionsenzyme Troubleshooting Guides Troubleshooting Guide for Cloning Restriction Enzyme Troubleshooting Guide Troubleshooting... 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