{"id":47255,"date":"2024-06-25T10:58:12","date_gmt":"2024-06-25T08:58:12","guid":{"rendered":"https:\/\/www.neb-online.de\/?page_id=47255"},"modified":"2024-09-27T14:00:51","modified_gmt":"2024-09-27T12:00:51","slug":"unleash-innovation-in-rolling-circle-amplification","status":"publish","type":"page","link":"https:\/\/www.neb-online.de\/en\/pcr-and-dna-amplification\/isothermal-amplification\/unleash-innovation-in-rolling-circle-amplification\/","title":{"rendered":"phi29-XT Rolling Circle Amplifikation Kit"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text css=&#8221;.vc_custom_1727438138486{margin-bottom: 0px !important;border-bottom-width: 0px !important;padding-bottom: 10px !important;}&#8221;]<\/p>\n<h2>Unleash Innovation in Rolling Circle Amplification<\/h2>\n<p>Rolling circle amplification (RCA) is an isothermal amplification method to continuously amplify circular DNA by generating long, repetitive copies of the circular sequence.<\/p>\n<p>The phi29-XT DNA Polymerase is an engineered polymerase with improved thermostability and sensitivity generating higher yield in a shorter reaction time than wild-type phi29 DNA Polymerase.[\/vc_column_text]<a class=\"btn btn-standard form-btn\" target=\"_blank\" href=\"https:\/\/www.neb.com\/m0269\" style=\"margin-right:auto;\">Learn more<\/a>[\/vc_column][vc_column width=&#8221;1\/2&#8243;]<div class=\"vbox\">\n\t\t\t\t<span class=\"vhead\">Advantages:<\/span>\n\t\t\t\t\t<ul class=\"vlist\">\n\t\t\t\t\t\t<li><span>High yield in a short reaction time<br \/><\/span><\/li><li><span>Amplify from 1 fg circular DNA input<br \/><\/span><\/li><li><span>Flexible protocol for various input materials<br \/><\/span><\/li><li><span>Enables multiple downstream applications without further processing steps<br \/><\/span><\/li><li><span>Kit includes dNTPs and exonuclease-resistant random primers<\/span><\/li>\n\t\t\t\t\t<\/ul>\n\t\t\t<\/div>[\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text css=&#8221;.vc_custom_1718890533458{margin-bottom: 0px !important;border-bottom-width: 0px !important;padding-bottom: 10px !important;}&#8221;]The phi29-XT RCA Kit includes everything needed for sensitive and robust rolling circle amplification The kit features the phi29-XT DNA Polymerase. Also included are dNTPs and exonuclease-resistant random primers (containing phosphorothioate bonds) to universally amplify circular DNA sequences. Input material can be purified circular DNA (single or double-stranded) or direct from liquid media culture, agar plate colonies, and glycerol stocks without the need for DNA extraction. RCA products can be used directly in downstream applications such as DNA sequencing, cell-free DNA enrichment, cell-free protein expression, and DNA biosensors.[\/vc_column_text]<a class=\"btn btn-standard form-btn\" target=\"_blank\" href=\"https:\/\/www.neb.com\/e1603\" style=\"margin-right:auto;\">Learn more<\/a>[\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_column_text css=&#8221;.vc_custom_1719301483494{margin-bottom: 0px !important;border-bottom-width: 0px !important;padding-bottom: 0px !important;}&#8221;]<strong>The phi29-XT RCA Kit<\/strong>[\/vc_column_text][vc_single_image source=&#8221;external_link&#8221; custom_src=&#8221;https:\/\/www.neb.com\/en\/-\/media\/nebus\/page-images\/products\/isothermal-amplification\/e1603_phi29_xt_rca_workflow_applications_0223.png&#8221; css=&#8221;.vc_custom_1718889845400{margin-top: 0px !important;border-top-width: 1px !important;border-right-width: 1px !important;border-bottom-width: 1px !important;border-left-width: 1px !important;padding-top: 10px !important;border-left-color: #e0e0e0 !important;border-left-style: solid !important;border-right-color: #e0e0e0 !important;border-right-style: solid !important;border-top-color: #e0e0e0 !important;border-top-style: solid !important;border-bottom-color: #e0e0e0 !important;border-bottom-style: solid !important;border-radius: 1px !important;}&#8221;][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;.vc_custom_1718890581471{margin-bottom: 0px !important;border-bottom-width: 0px !important;padding-bottom: 10px !important;}&#8221;]View the Enhanced Rolling Circle Amplification Performance with a Newly Engineered phi29 DNA Polymerase NEB TV webinar on demand.[\/vc_column_text]<a class=\"btn btn-standard form-btn\" target=\"_blank\" href=\"https:\/\/event.on24.com\/wcc\/r\/4195013\/ED995A61374C06F0801F71D46AE14164?partnerref=email1\" style=\"margin-right:auto;\">View webinar<\/a>[\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text css=&#8221;.vc_custom_1727438138486{margin-bottom: 0px !important;border-bottom-width: 0px !important;padding-bottom: 10px !important;}&#8221;] Unleash Innovation in Rolling Circle Amplification Rolling circle amplification (RCA) is an isothermal amplification method to continuously amplify circular DNA by generating long, repetitive copies of the circular sequence. The phi29-XT DNA Polymerase is an engineered polymerase with improved thermostability and sensitivity generating higher yield in&#8230;  <a class=\"excerpt-read-more\" href=\"https:\/\/www.neb-online.de\/en\/pcr-and-dna-amplification\/isothermal-amplification\/unleash-innovation-in-rolling-circle-amplification\/\" title=\"Read phi29-XT Rolling Circle Amplifikation Kit\">Read more &raquo;<\/a><\/p>\n","protected":false},"author":18,"featured_media":41831,"parent":2141,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-sidebar.php","meta":{"footnotes":""},"class_list":["post-47255","page","type-page","status-publish","has-post-thumbnail","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.1.1 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>phi29-XT Rolling Circle Amplifikation Kit - New England Biolabs GmbH<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.neb-online.de\/en\/pcr-and-dna-amplification\/isothermal-amplification\/unleash-innovation-in-rolling-circle-amplification\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"phi29-XT Rolling Circle Amplifikation Kit - New England Biolabs GmbH\" \/>\n<meta property=\"og:description\" content=\"[vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text css=&#8221;.vc_custom_1727438138486{margin-bottom: 0px !important;border-bottom-width: 0px !important;padding-bottom: 10px !important;}&#8221;] Unleash Innovation in Rolling Circle Amplification Rolling circle amplification (RCA) is an isothermal amplification method to continuously amplify circular DNA by generating long, repetitive copies of the circular sequence. The phi29-XT DNA Polymerase is an engineered polymerase with improved thermostability and sensitivity generating higher yield in... 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New England Biolabs GmbH","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.neb-online.de\/en\/pcr-and-dna-amplification\/isothermal-amplification\/unleash-innovation-in-rolling-circle-amplification\/","og_locale":"en_US","og_type":"article","og_title":"phi29-XT Rolling Circle Amplifikation Kit - New England Biolabs GmbH","og_description":"[vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text css=&#8221;.vc_custom_1727438138486{margin-bottom: 0px !important;border-bottom-width: 0px !important;padding-bottom: 10px !important;}&#8221;] Unleash Innovation in Rolling Circle Amplification Rolling circle amplification (RCA) is an isothermal amplification method to continuously amplify circular DNA by generating long, repetitive copies of the circular sequence. 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