{"id":47788,"date":"2024-09-17T10:05:02","date_gmt":"2024-09-17T08:05:02","guid":{"rendered":"https:\/\/www.neb-online.de\/pcr-and-dna-amplification\/isothermal-amplification\/phi29-xt-whole-genome-amplification-kit\/"},"modified":"2024-09-17T10:12:27","modified_gmt":"2024-09-17T08:12:27","slug":"phi29-xt-whole-genome-amplification-kit","status":"publish","type":"page","link":"https:\/\/www.neb-online.de\/en\/pcr-and-dna-amplification\/isothermal-amplification\/phi29-xt-whole-genome-amplification-kit\/","title":{"rendered":"phi29-XT Whole Genome Amplification Kit"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column width=&#8221;2\/3&#8243;][vc_column_text css=&#8221;.vc_custom_1726560626437{margin-bottom: 0px !important;border-bottom-width: 0px !important;padding-bottom: 10px !important;}&#8221;]<\/p>\n<h2>Reliable isothermal amplification of whole genomes<\/h2>\n<p>The phi29-XT WGA Kit is optimized for sensitive, fast, and robust whole genome amplification (WGA), an isothermal method for amplification of an entire genome, starting from femtogram quantities of DNA and resulting in microgram quantities of amplified products. The kit features phi29-XT DNA Polymerase, an engineered polymerase with improved thermostability, sensitivity, and capable of generating a higher yield in a shorter reaction time than wild-type phi29 DNA Polymerase. Also included are exonuclease-resistant random primers (containing phosphorothioate bonds) to universally amplify any DNA sequence, neutralization buffer, and dNTPs. Input material can be purified genomic DNA, cells, or microbiomes. WGA enables several downstream applications, including single cell whole genome sequencing (WGS), genomic DNA enrichment, and environmental DNA (eDNA) enrichment and sequencing.[\/vc_column_text][vc_single_image source=&#8221;external_link&#8221; external_img_size=&#8221;400x92px&#8221; custom_src=&#8221;https:\/\/www.neb.com\/en\/-\/media\/nebus\/page-images\/products\/isothermal-amplification\/e1604_phi29_xt_wga_workflow_timetemp_0524.png&#8221; css=&#8221;.vc_custom_1726560429274{margin-top: 0px !important;padding-top: 10px !important;border-radius: 1px !important;}&#8221;][\/vc_column][vc_column width=&#8221;1\/3&#8243;]<div class=\"vbox\">\n\t\t\t\t<span class=\"vhead\">Advantages:<\/span>\n\t\t\t\t\t<ul class=\"vlist\">\n\t\t\t\t\t\t<li><span>Powered by phi29-XT DNA Polymerase, an engineered polymerase with improved thermostability and sensitivity<br \/><\/span><\/li><li><span>High sensitivity: amplify from as little as 10 fg of input DNA<br \/><\/span><\/li><li><span>Flexible protocol offers compatibility with different types of sample input material<br \/><\/span><\/li><li><span>Simple workflow produces high yield in a short reaction time (less than 2 hours)<br \/><\/span><\/li><li><span>Whole genome representation for human and microbial DNA<br \/><\/span><\/li><li><span>Enables multiple downstream applications without further processing steps<\/span><\/li>\n\t\t\t\t\t<\/ul>\n\t\t\t<\/div>[\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;.vc_custom_1726560666195{margin-bottom: 0px !important;border-bottom-width: 0px !important;padding-bottom: 10px !important;}&#8221;]<\/p>\n<h2><strong>Overview of the phi29-XT WGA Kit<\/strong><\/h2>\n<p>[\/vc_column_text][vc_single_image source=&#8221;external_link&#8221; custom_src=&#8221;https:\/\/www.neb.com\/en\/-\/media\/nebus\/page-images\/products\/isothermal-amplification\/e1604_phi29_xt_wga_workflow_applications_0524.png&#8221; css=&#8221;.vc_custom_1726559773916{margin-bottom: 20px !important;border-top-width: 1px !important;border-right-width: 1px !important;border-bottom-width: 1px !important;border-left-width: 1px !important;padding-top: 0px !important;padding-bottom: 0px !important;border-left-color: #e0e0e0 !important;border-left-style: solid !important;border-right-color: #e0e0e0 !important;border-right-style: solid !important;border-top-color: #e0e0e0 !important;border-top-style: solid !important;border-bottom-color: #e0e0e0 !important;border-bottom-style: solid !important;border-radius: 1px !important;}&#8221;]<a class=\"btn btn-standard form-btn\" target=\"_blank\" href=\"https:\/\/www.neb.com\/e1604\" style=\"margin-right:auto;\">Learn more<\/a>[\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n<h3><strong>Available Products<\/strong><\/h3>\n<table style=\"width: 760px;\" border=\"0\" cellpadding=\"5\">\n<tbody>\n<tr style=\"background-color: #f3f3f3;\">\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"400\" height=\"40px\">PRODUCT<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"88\">Art.Nr.:<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"100\">SIZE<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" colspan=\"2\">Info<\/th>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">phi29-XT WGA Kit<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E1604S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">25 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/E1604@\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb.com\/en\/E1604\" target=\"blank\" rel=\"noopener\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"NEB Info_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">phi29-XT WGA Kit<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E1604L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/E1604@\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"NEB Shop_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\"><a href=\"https:\/\/www.neb.com\/en\/E1604\" target=\"blank\" rel=\"noopener\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"NEB Info_icon\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><span class=\"bildunterschrift\" style=\"float: right;\"><i>As of: 17.09.2024<\/i><\/span>[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row][vc_column width=&#8221;2\/3&#8243;][vc_column_text css=&#8221;.vc_custom_1726560626437{margin-bottom: 0px !important;border-bottom-width: 0px !important;padding-bottom: 10px !important;}&#8221;] Reliable isothermal amplification of whole genomes The phi29-XT WGA Kit is optimized for sensitive, fast, and robust whole genome amplification (WGA), an isothermal method for amplification of an entire genome, starting from femtogram quantities of DNA and resulting in microgram quantities of amplified products. The kit&#8230;  <a class=\"excerpt-read-more\" href=\"https:\/\/www.neb-online.de\/en\/pcr-and-dna-amplification\/isothermal-amplification\/phi29-xt-whole-genome-amplification-kit\/\" title=\"Read phi29-XT Whole Genome Amplification Kit\">Read more &raquo;<\/a><\/p>\n","protected":false},"author":18,"featured_media":41831,"parent":2141,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-sidebar.php","meta":{"footnotes":""},"class_list":["post-47788","page","type-page","status-publish","has-post-thumbnail","hentry","seitenkategorie-pcr-dna-amplifikation"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.5 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>phi29-XT Whole Genome Amplification Kit - New England Biolabs GmbH<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.neb-online.de\/en\/pcr-and-dna-amplification\/isothermal-amplification\/phi29-xt-whole-genome-amplification-kit\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"phi29-XT Whole Genome Amplification Kit - New England Biolabs GmbH\" \/>\n<meta property=\"og:description\" content=\"[vc_row][vc_column width=&#8221;2\/3&#8243;][vc_column_text css=&#8221;.vc_custom_1726560626437{margin-bottom: 0px !important;border-bottom-width: 0px !important;padding-bottom: 10px !important;}&#8221;] Reliable isothermal amplification of whole genomes The phi29-XT WGA Kit is optimized for sensitive, fast, and robust whole genome amplification (WGA), an isothermal method for amplification of an entire genome, starting from femtogram quantities of DNA and resulting in microgram quantities of amplified products. The kit... Read more &raquo;\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.neb-online.de\/en\/pcr-and-dna-amplification\/isothermal-amplification\/phi29-xt-whole-genome-amplification-kit\/\" \/>\n<meta property=\"og:site_name\" content=\"New England Biolabs GmbH\" \/>\n<meta property=\"article:modified_time\" content=\"2024-09-17T08:12:27+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2019\/01\/NEB_PCR_Header_dnaamplification_760x200.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"760\" \/>\n\t<meta property=\"og:image:height\" content=\"200\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"3 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/www.neb-online.de\\\/en\\\/pcr-and-dna-amplification\\\/isothermal-amplification\\\/phi29-xt-whole-genome-amplification-kit\\\/\",\"url\":\"https:\\\/\\\/www.neb-online.de\\\/en\\\/pcr-and-dna-amplification\\\/isothermal-amplification\\\/phi29-xt-whole-genome-amplification-kit\\\/\",\"name\":\"phi29-XT Whole Genome Amplification Kit - New England Biolabs GmbH\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/www.neb-online.de\\\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\\\/\\\/www.neb-online.de\\\/en\\\/pcr-and-dna-amplification\\\/isothermal-amplification\\\/phi29-xt-whole-genome-amplification-kit\\\/#primaryimage\"},\"image\":{\"@id\":\"https:\\\/\\\/www.neb-online.de\\\/en\\\/pcr-and-dna-amplification\\\/isothermal-amplification\\\/phi29-xt-whole-genome-amplification-kit\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/www.neb-online.de\\\/wp-content\\\/uploads\\\/2019\\\/01\\\/NEB_PCR_Header_dnaamplification_760x200.jpg\",\"datePublished\":\"2024-09-17T08:05:02+00:00\",\"dateModified\":\"2024-09-17T08:12:27+00:00\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/www.neb-online.de\\\/en\\\/pcr-and-dna-amplification\\\/isothermal-amplification\\\/phi29-xt-whole-genome-amplification-kit\\\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/www.neb-online.de\\\/en\\\/pcr-and-dna-amplification\\\/isothermal-amplification\\\/phi29-xt-whole-genome-amplification-kit\\\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/www.neb-online.de\\\/en\\\/pcr-and-dna-amplification\\\/isothermal-amplification\\\/phi29-xt-whole-genome-amplification-kit\\\/#primaryimage\",\"url\":\"https:\\\/\\\/www.neb-online.de\\\/wp-content\\\/uploads\\\/2019\\\/01\\\/NEB_PCR_Header_dnaamplification_760x200.jpg\",\"contentUrl\":\"https:\\\/\\\/www.neb-online.de\\\/wp-content\\\/uploads\\\/2019\\\/01\\\/NEB_PCR_Header_dnaamplification_760x200.jpg\",\"width\":760,\"height\":200},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/www.neb-online.de\\\/en\\\/pcr-and-dna-amplification\\\/isothermal-amplification\\\/phi29-xt-whole-genome-amplification-kit\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\\\/\\\/www.neb-online.de\\\/en\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"PCR and DNA Amplification\",\"item\":\"https:\\\/\\\/www.neb-online.de\\\/en\\\/pcr-and-dna-amplification\\\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"Isothermal Amplification\",\"item\":\"https:\\\/\\\/www.neb-online.de\\\/en\\\/pcr-and-dna-amplification\\\/isothermal-amplification\\\/\"},{\"@type\":\"ListItem\",\"position\":4,\"name\":\"phi29-XT Whole Genome Amplification Kit\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/www.neb-online.de\\\/#website\",\"url\":\"https:\\\/\\\/www.neb-online.de\\\/\",\"name\":\"New England Biolabs GmbH\",\"description\":\"Enabling Technologies in the Life Sciences\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/www.neb-online.de\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"phi29-XT Whole Genome Amplification Kit - New England Biolabs GmbH","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.neb-online.de\/en\/pcr-and-dna-amplification\/isothermal-amplification\/phi29-xt-whole-genome-amplification-kit\/","og_locale":"en_US","og_type":"article","og_title":"phi29-XT Whole Genome Amplification Kit - New England Biolabs GmbH","og_description":"[vc_row][vc_column width=&#8221;2\/3&#8243;][vc_column_text css=&#8221;.vc_custom_1726560626437{margin-bottom: 0px !important;border-bottom-width: 0px !important;padding-bottom: 10px !important;}&#8221;] Reliable isothermal amplification of whole genomes The phi29-XT WGA Kit is optimized for sensitive, fast, and robust whole genome amplification (WGA), an isothermal method for amplification of an entire genome, starting from femtogram quantities of DNA and resulting in microgram quantities of amplified products. The kit... Read more &raquo;","og_url":"https:\/\/www.neb-online.de\/en\/pcr-and-dna-amplification\/isothermal-amplification\/phi29-xt-whole-genome-amplification-kit\/","og_site_name":"New England Biolabs GmbH","article_modified_time":"2024-09-17T08:12:27+00:00","og_image":[{"width":760,"height":200,"url":"https:\/\/www.neb-online.de\/wp-content\/uploads\/2019\/01\/NEB_PCR_Header_dnaamplification_760x200.jpg","type":"image\/jpeg"}],"twitter_misc":{"Est. reading time":"3 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/www.neb-online.de\/en\/pcr-and-dna-amplification\/isothermal-amplification\/phi29-xt-whole-genome-amplification-kit\/","url":"https:\/\/www.neb-online.de\/en\/pcr-and-dna-amplification\/isothermal-amplification\/phi29-xt-whole-genome-amplification-kit\/","name":"phi29-XT Whole Genome Amplification Kit - New England Biolabs GmbH","isPartOf":{"@id":"https:\/\/www.neb-online.de\/#website"},"primaryImageOfPage":{"@id":"https:\/\/www.neb-online.de\/en\/pcr-and-dna-amplification\/isothermal-amplification\/phi29-xt-whole-genome-amplification-kit\/#primaryimage"},"image":{"@id":"https:\/\/www.neb-online.de\/en\/pcr-and-dna-amplification\/isothermal-amplification\/phi29-xt-whole-genome-amplification-kit\/#primaryimage"},"thumbnailUrl":"https:\/\/www.neb-online.de\/wp-content\/uploads\/2019\/01\/NEB_PCR_Header_dnaamplification_760x200.jpg","datePublished":"2024-09-17T08:05:02+00:00","dateModified":"2024-09-17T08:12:27+00:00","breadcrumb":{"@id":"https:\/\/www.neb-online.de\/en\/pcr-and-dna-amplification\/isothermal-amplification\/phi29-xt-whole-genome-amplification-kit\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.neb-online.de\/en\/pcr-and-dna-amplification\/isothermal-amplification\/phi29-xt-whole-genome-amplification-kit\/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.neb-online.de\/en\/pcr-and-dna-amplification\/isothermal-amplification\/phi29-xt-whole-genome-amplification-kit\/#primaryimage","url":"https:\/\/www.neb-online.de\/wp-content\/uploads\/2019\/01\/NEB_PCR_Header_dnaamplification_760x200.jpg","contentUrl":"https:\/\/www.neb-online.de\/wp-content\/uploads\/2019\/01\/NEB_PCR_Header_dnaamplification_760x200.jpg","width":760,"height":200},{"@type":"BreadcrumbList","@id":"https:\/\/www.neb-online.de\/en\/pcr-and-dna-amplification\/isothermal-amplification\/phi29-xt-whole-genome-amplification-kit\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/www.neb-online.de\/en\/"},{"@type":"ListItem","position":2,"name":"PCR and DNA Amplification","item":"https:\/\/www.neb-online.de\/en\/pcr-and-dna-amplification\/"},{"@type":"ListItem","position":3,"name":"Isothermal Amplification","item":"https:\/\/www.neb-online.de\/en\/pcr-and-dna-amplification\/isothermal-amplification\/"},{"@type":"ListItem","position":4,"name":"phi29-XT Whole Genome Amplification Kit"}]},{"@type":"WebSite","@id":"https:\/\/www.neb-online.de\/#website","url":"https:\/\/www.neb-online.de\/","name":"New England Biolabs GmbH","description":"Enabling Technologies in the Life Sciences","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/www.neb-online.de\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"}]}},"_links":{"self":[{"href":"https:\/\/www.neb-online.de\/en\/wp-json\/wp\/v2\/pages\/47788","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.neb-online.de\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.neb-online.de\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.neb-online.de\/en\/wp-json\/wp\/v2\/users\/18"}],"replies":[{"embeddable":true,"href":"https:\/\/www.neb-online.de\/en\/wp-json\/wp\/v2\/comments?post=47788"}],"version-history":[{"count":2,"href":"https:\/\/www.neb-online.de\/en\/wp-json\/wp\/v2\/pages\/47788\/revisions"}],"predecessor-version":[{"id":47790,"href":"https:\/\/www.neb-online.de\/en\/wp-json\/wp\/v2\/pages\/47788\/revisions\/47790"}],"up":[{"embeddable":true,"href":"https:\/\/www.neb-online.de\/en\/wp-json\/wp\/v2\/pages\/2141"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.neb-online.de\/en\/wp-json\/wp\/v2\/media\/41831"}],"wp:attachment":[{"href":"https:\/\/www.neb-online.de\/en\/wp-json\/wp\/v2\/media?parent=47788"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}