{"id":4960,"date":"2015-06-15T08:39:14","date_gmt":"2015-06-15T08:39:14","guid":{"rendered":"http:\/\/www.neb-online.de\/?page_id=4960"},"modified":"2023-12-19T13:57:11","modified_gmt":"2023-12-19T12:57:11","slug":"nebnext-library-quant-kit-for-illumina","status":"publish","type":"page","link":"https:\/\/www.neb-online.de\/en\/next-generation-sequencing\/nebnext-library-quant-kit-for-illumina\/","title":{"rendered":"NEBNext Library Quant Kit for Illumina"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column][vc_basic_grid post_type=&#8221;post&#8221; max_items=&#8221;10&#8243; element_width=&#8221;12&#8243; gap=&#8221;15&#8243; item=&#8221;4123&#8243; taxonomies=&#8221;129&#8243; grid_id=&#8221;vc_gid:1702990565235-5cbd29f7-035a-4&#8243;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]Accurate quantitation of next-generation sequencing libraries is essential for maximizing data output and quality from each sequencing run.<\/p>\n<p>Furthermore, for Illumina sequencing, accurate quantitation of libraries is critical to achieve optimal cluster densities, a requirement for optimal sequence output.<\/p>\n<p>qPCR is considered to be the most accurate and effective method of library quantitation, providing considerably higher consistency and reproducibility of quantitation.<\/p>\n<p>Amplification-based methods quantitate only those molecules that contain both adaptor sequences, thereby providing a more accurate estimate of the concentration of the library molecules that can be sequenced.[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243;]<div class=\"vbox\">\n\t\t\t\t<span class=\"vhead\">Advantages:<\/span>\n\t\t\t\t\t<ul class=\"vlist\">\n\t\t\t\t\t\t<li><span>Be confident in your quant values, as our kit provides more accurate and reproducible results than other methods and kits.<br \/><\/span><\/li><li><span>Get up and running quickly with our easy-to-use kit, containing Library Dilution Buffer, optimized master mix and ROX dye.<br \/><\/span><\/li><li><span>Enjoy the flexibility to use either four or six standards (included).<br \/><\/span><\/li><li><span>Simplify your reaction setup with fewer pipetting steps and a single extension time for all libraries.<br \/><\/span><\/li><li><span>Use with all your libraries, regardless of insert size, GC content and preparation method.<br \/><\/span><\/li><li><span>Save money with our value pricing.<\/span><\/li>\n\t\t\t\t\t<\/ul>\n\t\t\t<\/div>[vc_column_text css=&#8221;.vc_custom_1567151190718{margin-top: 20px !important;}&#8221;]<\/p>\n<p style=\"text-align: center;\"><strong>Get a free NEBNext Library Quant Kit sample!<\/strong><\/p>\n<p>[\/vc_column_text]<a class=\"btn btn-standard form-btn\"  href=\"#formular\" style=\"margin-left:auto;margin-right:auto;\">Sample request form<\/a>[\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]The NEBNext Library Quant Kit delivers significant improvements to qPCR-based library quantitation for next gen sequencing.[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=&#8221;custom&#8221; accent_color=&#8221;#eb7e2d&#8221;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;2\/3&#8243;][vc_column_text]<\/p>\n<div class=\"wpb_text_column wpb_content_element \">\n<div class=\"wpb_wrapper\">\n<p style=\"text-align: center;\">Feature Article:<\/p>\n<p style=\"text-align: center;\"><strong>\u201eThe Quantitation Question: How does accurate<br \/>\nlibrary <\/strong><strong>quantitation influence sequencing?\u201c<\/strong><\/p>\n<\/div>\n<\/div>\n<p>[\/vc_column_text]<a class=\"btn btn-standard form-btn\" target=\"_blank\" href=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/10\/FEATURE_ARTICLE_NEBNext_QuantKit.pdf\" style=\"margin-left:auto;margin-right:auto;\">Download PDF<\/a>[\/vc_column][vc_column width=&#8221;1\/3&#8243; css=&#8221;.vc_custom_1434466146403{padding-top: 0px !important;padding-right: 0px !important;padding-bottom: 0px !important;padding-left: 0px !important;}&#8221;][vc_single_image image=&#8221;6832&#8243; img_size=&#8221;full&#8221; alignment=&#8221;center&#8221;][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=&#8221;custom&#8221; accent_color=&#8221;#eb7e2d&#8221;][\/vc_column][\/vc_row][vc_row][vc_column]<div id=\"shorten-32836\" class=\"shorten\" style=\"max-height:300px;\">[vc_column_text]<\/p>\n<h2>With NEBNext, optimal cluster density is achieved from quantitated libraries with a broad range of library size and GC content.<\/h2>\n<p>[\/vc_column_text][vc_single_image image=&#8221;4967&#8243; img_size=&#8221;full&#8221; alignment=&#8221;center&#8221; img_link_large=&#8221;yes&#8221;][vc_column_text]<span class=\"bildunterschrift\">Libraries of 310\u2013963 bp from the indicated sources were quantitated using the NEBNext Library Quant Kit, then diluted to 8 pM and loaded onto a MiSeq<sup>\u00ae<\/sup> (v2 chemistry; MCS v2.4.1.3). Library concentrations ranged from 7\u2013120 nM, and resulting raw cluster density for all libraries was 965\u20131300 k\/mm2 (ave. =1199). Optimal cluster density was achieved using concentrations determined by the NEBNext Library Quant Kit for all library sizes.<\/span>[\/vc_column_text][vc_separator][vc_column_text]<\/p>\n<h2><span class=\"bildunterschrift\">Greater reproducibility of library quantitation with the NEBNext Library Quant Kit<\/span><\/h2>\n<p>[\/vc_column_text][vc_single_image image=&#8221;4978&#8243; img_size=&#8221;full&#8221; alignment=&#8221;center&#8221; img_link_large=&#8221;yes&#8221;][vc_column_text]<span class=\"bildunterschrift\">Three 340\u2013400 bp libraries were quantitated by 4 different users 2\u20134 times using either the NEBNext or KapaTM Library Quantification Kit (Universal). A notable improvement in quantitation consistency was observed for concentrations determined by the NEBNext Kit (orange) versus those from the Kapa kit (gray).\u00a0<\/span>[\/vc_column_text][vc_separator][vc_column_text]<\/p>\n<h2>The NEBNext Library Quant Kit values enable optimal cluster densities<\/h2>\n<p>[\/vc_column_text][vc_single_image image=&#8221;4979&#8243; img_size=&#8221;full&#8221; alignment=&#8221;center&#8221; img_link_large=&#8221;yes&#8221;][vc_column_text]<span class=\"bildunterschrift\">Seven different libraries were quantitated using either the NEBNext Library Quant Kit (orange) or the Kapa Library Quantification Kit (Universal) (gray). Undiluted library concentrations ranged from 2\u2013200 nM. Libraries were diluted to 8 pM and loaded onto a MiSeq\u00ae instrument (v2 chemistry; MCS v2.4.1.3). Libraries quantitated with the NEBNext kit resulted in a raw cluster density average of 1160 k\/mm2, directly in the optimal range of 900\u20131300 k\/mm2. In contrast, libraries loaded based on the Kapa quantitation averaged only 660 k\/mm2.\u00a0<\/span>[\/vc_column_text]<\/div><a href=\"#\" id=\"btn-shorten-32836\" class=\"shorten-btn\">Read more<\/a><style>\n\t\t#shorten-32836::after{\n\t\t\tbackground: linear-gradient(to bottom, rgba(255,255,255,0), #ffffff);\n\t\t\tbackground: -moz-linear-gradient(top, rgba(255,255,255,0), #ffffff);\n\t\t\tbackground: -webkit-linear-gradient(top, rgba(255,255,255,0), #ffffff);\n\t\t}\n\t<\/style><script>\n\t\t\t\t\t\t\t\t\tjQuery(document).ready(function($) {\n\t\t\t\t\t\t\t\t\t\t$(\"#btn-shorten-32836\").click(function(event) {\n\t\t\t\t\t\t\t\t\t\t\tevent.preventDefault();\n\t\t\t\t\t\t\t\t\t\t\tif($(\"#shorten-32836\").hasClass(\"open\")){\n\t\t\t\t\t\t\t\t\t\t\t\t$(\"#shorten-32836\").removeClass(\"open\");\n\t\t\t\t\t\t\t\t\t\t\t\t$(\"#btn-shorten-32836\").html(\"Read more\");\n\t\t\t\t\t\t\t\t\t\t\t} else {\n\t\t\t\t\t\t\t\t\t\t\t\t$(\"#shorten-32836\").addClass(\"open\");\n\t\t\t\t\t\t\t\t\t\t\t\t$(\"#btn-shorten-32836\").html(\"Read less\");\n\t\t\t\t\t\t\t\t\t\t\t}\n\t\t\t\t\t\t\t\t\t\t});\n\t\t\t\t\t\t\t\t\t});\n\t\t\t\t\t\t\t<\/script>[\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=&#8221;custom&#8221; accent_color=&#8221;#eb7e2d&#8221;][\/vc_column][\/vc_row][vc_row bwd_show_when_type=&#8221;before&#8221; bwd_show_when_date=&#8221;12\/31\/2023 23:00&#8243; bwd_tbc4vd_show_weekday=&#8221;&#8221;][vc_column][vc_column_text]<\/p>\n<h2>Available products<\/h2>\n<table style=\"width: 760px;\" border=\"0\" cellpadding=\"5\">\n<tbody>\n<tr style=\"background-color: #f3f3f3; font-size: 16px;\">\n<th style=\"border-bottom: solid #eb7e2d;\" align=\"left\" width=\"400\" height=\"40px\">PRODUCT<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"88\">Prod. #<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"100\">SIZE<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" colspan=\"3\">PRICE<\/th>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">NEBNext<sup>\u00ae<\/sup> Library Quant Kit for Illumina<sup>\u00ae<\/sup><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E7630S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>112 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/E7630\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/e7630\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">NEBNext<sup>\u00ae<\/sup> Library Quant Kit for Illumina<sup>\u00ae<\/sup><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E7630L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">500 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>480 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/E7630\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/e7630\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p style=\"text-align: right;\"><span class=\"bildunterschrift\">As of: 01.01.2023<\/span><\/p>\n<p><span id=\"result_box\" class=\"\" lang=\"en\"><span class=\"\">NEBNext products are available at a permanent low price that can not be further discounted.<\/span><\/span>[\/vc_column_text][\/vc_column][\/vc_row][vc_row bwd_show_when_type=&#8221;after&#8221; bwd_show_when_date=&#8221;12\/31\/2023 23:00&#8243; bwd_tbc4vd_show_weekday=&#8221;&#8221;][vc_column][vc_column_text]<\/p>\n<h2>Available products<\/h2>\n<table style=\"width: 760px;\" border=\"0\" cellpadding=\"5\">\n<tbody>\n<tr style=\"background-color: #f3f3f3; font-size: 16px;\">\n<th style=\"border-bottom: solid #eb7e2d;\" align=\"left\" width=\"400\" height=\"40px\">PRODUCT<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"88\">Prod. #<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"100\">SIZE<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" colspan=\"2\">INFO<\/th>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">NEBNext<sup>\u00ae<\/sup> Library Quant Kit for Illumina<sup>\u00ae<\/sup><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E7630S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">100 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/E7630\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/e7630\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">NEBNext<sup>\u00ae<\/sup> Library Quant Kit for Illumina<sup>\u00ae<\/sup><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E7630L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">500 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/E7630\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/e7630\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p style=\"text-align: right;\"><span class=\"bildunterschrift\">As of: 01.01.2024<\/span><\/p>\n<p><span id=\"result_box\" class=\"\" lang=\"en\"><span class=\"\">NEBNext products are available at a permanent low price that can not be further discounted.<\/span><\/span>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=&#8221;custom&#8221; accent_color=&#8221;#eb7e2d&#8221; 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!important;}\n  input[type=\"checkbox\"] {min-width:20px !important; min-height:20px !important;}\n    input[type=\"radio\"] {min-width:20px !important; min-height:20px !important;}\n<\/style><\/div>\n<form method=\"post\" action=\"https:\/\/newenglandbiolabs.tfaforms.net\/api_v2\/rest\/workflow\/processor\" class=\"hintsBelow labelsAbove\" id=\"338301\">\n<div class=\"htmlSection\" id=\"tfa_77\"><div class=\"htmlContent\" id=\"tfa_77-HTML\"><h2>Request a free <span style=\"color:#eb7e2d;\">NEBNext<\/span> NGS Library Prep sample!<\/h2><div><a target=\"_blank\" href=\"https:\/\/nebnextselector.neb.com\/\">Click here<\/a> for help choosing which NEBNext kit is right for you.&nbsp;<\/div><\/div><\/div>\n<div class=\"htmlSection\" id=\"tfa_7\"><div class=\"htmlContent\" id=\"tfa_7-HTML\"><br>Fields marked <span style=\"color: red;\">*<\/span> are required.<\/div><\/div>\n<div class=\"oneField field-container-D    \" id=\"tfa_43-D\" role=\"group\" aria-labelledby=\"tfa_43-L\" data-tfa-labelledby=\"-L tfa_43-L\">\n<label id=\"tfa_43-L\" class=\"label preField \" data-tfa-check-label-for=\"tfa_43\">NEBNext DNA &amp; RNA Library Prep Kits<\/label><br><div class=\"inputWrapper\"><span id=\"tfa_43\" class=\"choices vertical \"><span class=\"oneChoice\"><input type=\"checkbox\" value=\"tfa_118\" class=\"calc-NEBNEXTKITS calcval-E3325G\\u0020NEBNext\\u0020UltraExpress\\u0020DNA\\u0020Library\\u0020Prep\\u0020Kit\\n\" id=\"tfa_118\" name=\"tfa_118\" aria-labelledby=\"tfa_118-L\" data-tfa-labelledby=\"tfa_43-L tfa_118-L\" data-tfa-parent-id=\"tfa_43\"><label class=\"label postField\" id=\"tfa_118-L\" for=\"tfa_118\"><span class=\"input-checkbox-faux\"><\/span>NEBNext UltraExpress DNA Library Prep Kit | <a href=\"https:\/\/international.neb.com\/E3325\" target=\"_blank\">Info<\/a><\/label><\/span><span class=\"oneChoice\"><input type=\"checkbox\" value=\"tfa_120\" class=\"calc-NEBNEXTKITS calcval-E3340G\\u0020NEBNext\\u0020UltraExpress\\u0020DS\\u0020DNA\\u0020Library\\u0020Prep\\n\" id=\"tfa_120\" name=\"tfa_120\" aria-labelledby=\"tfa_120-L\" data-tfa-labelledby=\"tfa_43-L tfa_120-L\" data-tfa-parent-id=\"tfa_43\"><label class=\"label postField\" id=\"tfa_120-L\" for=\"tfa_120\"><span class=\"input-checkbox-faux\"><\/span>NEBNext UltraExpress FS DNA Library Prep Kit | <a target=\"_blank\" href=\"https:\/\/www.neb.com\/en\/E3340\">Info<\/a><\/label><\/span><span class=\"oneChoice\"><input type=\"checkbox\" value=\"tfa_119\" class=\"calc-NEBNEXTKITS calcval-E3330G\\u0020NEBNext\\u0020UltraExpress\\u0020RNA\\u0020Library\\u0020Prep\\u0020Kit\\n\" id=\"tfa_119\" name=\"tfa_119\" aria-labelledby=\"tfa_119-L\" data-tfa-labelledby=\"tfa_43-L tfa_119-L\" data-tfa-parent-id=\"tfa_43\"><label class=\"label postField\" id=\"tfa_119-L\" for=\"tfa_119\"><span class=\"input-checkbox-faux\"><\/span>NEBNext UltraExpress RNA Library Prep Kit | <a href=\"https:\/\/international.neb.com\/E3330\" target=\"_blank\">Info<\/a><\/label><\/span><span class=\"oneChoice\"><input type=\"checkbox\" value=\"tfa_44\" class=\"calc-NEBNEXTKITS calcval-E7645G\\u0020NEBNext\\u0020Ultra\\u0020II\\u0020DNA\\u0020Library\\u0020Prep\\u0020Kit\\u0020for\\u0020Illumina\\n\" id=\"tfa_44\" name=\"tfa_44\" aria-labelledby=\"tfa_44-L\" data-tfa-labelledby=\"tfa_43-L tfa_44-L\" data-tfa-parent-id=\"tfa_43\"><label class=\"label postField\" id=\"tfa_44-L\" for=\"tfa_44\"><span class=\"input-checkbox-faux\"><\/span>NEBNext Ultra II DNA Library Prep Kit for Illumina | <a href=\"https:\/\/international.neb.com\/E7645\" target=\"_blank\">Info<\/a><\/label><\/span><span class=\"oneChoice\"><input type=\"checkbox\" value=\"tfa_45\" class=\"calc-NEBNEXTKITS calcval-E7805G\\u0020NEBNext\\u0020Ultra\\u0020II\\u0020FS\\u0020DNA\\u0020Library\\u0020Prep\\u0020Kit\\u0020for\\u0020Illumina\\n\" id=\"tfa_45\" name=\"tfa_45\" aria-labelledby=\"tfa_45-L\" data-tfa-labelledby=\"tfa_43-L tfa_45-L\" data-tfa-parent-id=\"tfa_43\"><label class=\"label postField\" id=\"tfa_45-L\" for=\"tfa_45\"><span class=\"input-checkbox-faux\"><\/span>NEBNext Ultra II FS DNA Library Prep Kit for Illumina&nbsp;| <a href=\"https:\/\/international.neb.com\/E7805\" target=\"_blank\">Info<\/a><\/label><\/span><span class=\"oneChoice\"><input type=\"checkbox\" value=\"tfa_46\" class=\"calc-NEBNEXTKITS calcval-E7770G\\u0020NEBNext\\u0020Ultra\\u0020II\\u0020RNA\\u0020Library\\u0020Prep\\u0020Kit\\n\" id=\"tfa_46\" name=\"tfa_46\" aria-labelledby=\"tfa_46-L\" data-tfa-labelledby=\"tfa_43-L tfa_46-L\" data-tfa-parent-id=\"tfa_43\"><label class=\"label postField\" id=\"tfa_46-L\" for=\"tfa_46\"><span class=\"input-checkbox-faux\"><\/span>NEBNext Ultra II RNA Library Prep Kit&nbsp;| <a href=\"https:\/\/international.neb.com\/E7770\" target=\"_blank\">Info<\/a><\/label><\/span><span class=\"oneChoice\"><input type=\"checkbox\" value=\"tfa_47\" class=\"calc-NEBNEXTKITS calcval-E7760G\\u0020NEBNext\\u0020Ultra\\u0020II\\u0020Directional\\u0020RNA\\u0020Library\\u0020Prep\\u0020Kit\\n\" id=\"tfa_47\" name=\"tfa_47\" aria-labelledby=\"tfa_47-L\" data-tfa-labelledby=\"tfa_43-L tfa_47-L\" data-tfa-parent-id=\"tfa_43\"><label class=\"label postField\" id=\"tfa_47-L\" for=\"tfa_47\"><span class=\"input-checkbox-faux\"><\/span>NEBNext Ultra II Directional RNA Library Prep Kit&nbsp;| <a href=\"https:\/\/international.neb.com\/E7760\" target=\"_blank\">Info<\/a><\/label><\/span><span class=\"oneChoice\"><input type=\"checkbox\" value=\"tfa_122\" class=\"calc-NEBNEXTKITS calcval-E9705G\\u0020NEBNext\\u0020FS\\u0020DNA\\u0020Library\\u0020\\u0020Prep\\u0020Kit\\u0020for\\u0020MGI\\n\" id=\"tfa_122\" name=\"tfa_122\" aria-labelledby=\"tfa_122-L\" data-tfa-labelledby=\"tfa_43-L tfa_122-L\" data-tfa-parent-id=\"tfa_43\"><label class=\"label postField\" id=\"tfa_122-L\" for=\"tfa_122\"><span class=\"input-checkbox-faux\"><\/span>NEBNext FS DNA Library Prep Kit for MGI | <a target=\"_blank\" href=\"https:\/\/international.neb.com\/E9705\">Info<\/a><\/label><\/span><span class=\"oneChoice\"><input type=\"checkbox\" value=\"tfa_123\" class=\"calc-NEBNEXTKITS calcval-E9710G\\u0020NEBNext\\u0020RNA\\u0020Library\\u0020Prep\\u0020Kit\\u0020for\\u0020MGI\\n\" id=\"tfa_123\" name=\"tfa_123\" aria-labelledby=\"tfa_123-L\" data-tfa-labelledby=\"tfa_43-L tfa_123-L\" data-tfa-parent-id=\"tfa_43\"><label class=\"label postField\" id=\"tfa_123-L\" for=\"tfa_123\"><span class=\"input-checkbox-faux\"><\/span>NEBNext RNA Library Prep Kit for MGI | <a target=\"_blank\" href=\"https:\/\/international.neb.com\/E9710\">Info<\/a><\/label><\/span><span class=\"oneChoice\"><input type=\"checkbox\" value=\"tfa_48\" class=\"calc-NEBNEXTKITS calcval-E6420G\\u0020NEBNext\\u0020Single\\u0020Cell\/Low\\u0020Input\\u0020RNA\\u0020Library\\u0020Prep\\u0020Kit\\n\" id=\"tfa_48\" name=\"tfa_48\" aria-labelledby=\"tfa_48-L\" data-tfa-labelledby=\"tfa_43-L tfa_48-L\" data-tfa-parent-id=\"tfa_43\"><label class=\"label postField\" id=\"tfa_48-L\" for=\"tfa_48\"><span class=\"input-checkbox-faux\"><\/span>NEBNext Single Cell\/Low Input RNA Library Prep Kit&nbsp;| <a href=\"https:\/\/international.neb.com\/E6420\" target=\"_blank\">Info<\/a><\/label><\/span><span class=\"oneChoice\"><input type=\"checkbox\" value=\"tfa_125\" class=\"calc-NEBNEXTKITS calcval-E7300G\\u0020NEBNext\\u0020Enzymatic\\u0020Methyl-seq\\u0020v2\\u0020Kit\\n\" id=\"tfa_125\" name=\"tfa_125\" aria-labelledby=\"tfa_125-L\" data-tfa-labelledby=\"tfa_43-L tfa_125-L\" data-tfa-parent-id=\"tfa_43\"><label class=\"label postField\" id=\"tfa_125-L\" for=\"tfa_125\"><span class=\"input-checkbox-faux\"><\/span>NEBNext Enzymatic Methyl-seq v2 Kit | <a href=\"https:\/\/www.neb.com\/E8015\">Info<\/a><\/label><\/span><span class=\"oneChoice\"><input type=\"checkbox\" value=\"tfa_116\" class=\"calc-NEBNEXTKITS calcval-E6650G\\u0020NEBNext\\u0020FFPE\\u0020DNA\\u0020Library\\u0020Prep\\u0020Kit\\u0020Sample\\n\" id=\"tfa_116\" name=\"tfa_116\" aria-labelledby=\"tfa_116-L\" data-tfa-labelledby=\"tfa_43-L tfa_116-L\" data-tfa-parent-id=\"tfa_43\"><label class=\"label postField\" id=\"tfa_116-L\" for=\"tfa_116\"><span class=\"input-checkbox-faux\"><\/span>NEBNext FFPE DNA Library Prep Kit | <a target=\"_blank\" href=\"https:\/\/international.neb.com\/E6650\">Info<\/a><\/label><\/span><span class=\"oneChoice\"><input type=\"checkbox\" value=\"tfa_117\" class=\"calc-NEBNEXTKITS calcval-E6655G\\u0020NEBNext\\u0020UltraShear\\u0020FFPE\\u0020DNA\\u0020Library\\u0020Prep\\u0020Kit\\u0020Sample\\n\" id=\"tfa_117\" name=\"tfa_117\" aria-labelledby=\"tfa_117-L\" data-tfa-labelledby=\"tfa_43-L tfa_117-L\" data-tfa-parent-id=\"tfa_43\"><label class=\"label postField\" id=\"tfa_117-L\" for=\"tfa_117\"><span class=\"input-checkbox-faux\"><\/span>NEBNext UltraShear FFPE DNA Library Prep Kit | <a target=\"_blank\" href=\"https:\/\/international.neb.com\/E6655\">Info<\/a><\/label><\/span><span class=\"oneChoice\"><input type=\"checkbox\" value=\"tfa_127\" class=\"calc-NEBNEXTKITS calcval-E3420G\\u0020NEBNext\\u0020Low-bias\\u0020FFPE\\u0020Small\\u0020RNA\\u0020Library\\u0020Prep\\u0020Kit\\u0020Sample\\n\" id=\"tfa_127\" name=\"tfa_127\" aria-labelledby=\"tfa_127-L\" data-tfa-labelledby=\"tfa_43-L tfa_127-L\" data-tfa-parent-id=\"tfa_43\"><label class=\"label postField\" id=\"tfa_127-L\" for=\"tfa_127\"><span class=\"input-checkbox-faux\"><\/span>NEBNext Low-bias Small RNA Library Prep Kit | <a href=\"https:\/\/international.neb.com\/E3420\">Info<\/a><\/label><\/span><\/span><\/div>\n<\/div>\n<div class=\"oneField field-container-D    \" id=\"tfa_50-D\" role=\"group\" aria-labelledby=\"tfa_50-L\" data-tfa-labelledby=\"-L tfa_50-L\">\n<label id=\"tfa_50-L\" class=\"label preField \" data-tfa-check-label-for=\"tfa_50\"><div>NEBNext Modules and companion products<\/div><\/label><br><div class=\"inputWrapper\"><span id=\"tfa_50\" class=\"choices vertical \"><span class=\"oneChoice\"><input type=\"checkbox\" value=\"tfa_51\" class=\"calc-NEBNEXTMODULE calcval-E7750S\\u0020NEBNext\\u0020Globin\\u0020&amp;\\u0020rRNA\\u0020Depletion\\u0020Kit\\u0020(Human\/Mouse\/Rat)\\n\" id=\"tfa_51\" name=\"tfa_51\" aria-labelledby=\"tfa_51-L\" data-tfa-labelledby=\"tfa_50-L tfa_51-L\" data-tfa-parent-id=\"tfa_50\"><label class=\"label postField\" id=\"tfa_51-L\" for=\"tfa_51\"><span class=\"input-checkbox-faux\"><\/span>NEBNext Globin &amp; rRNA Depletion Kit (Human\/Mouse\/Rat) | <a href=\"https:\/\/international.neb.com\/E7750\" target=\"_blank\">Info<\/a><\/label><\/span><span class=\"oneChoice\"><input type=\"checkbox\" value=\"tfa_52\" class=\"calc-NEBNEXTMODULE calcval-E7400S\\u0020NEBNext\\u0020rRNA\\u0020Depletion\\u0020Kit\\u0020v2\\u0020(Human\/Mouse\/Rat)\\n\" id=\"tfa_52\" name=\"tfa_52\" aria-labelledby=\"tfa_52-L\" data-tfa-labelledby=\"tfa_50-L tfa_52-L\" data-tfa-parent-id=\"tfa_50\"><label class=\"label postField\" id=\"tfa_52-L\" for=\"tfa_52\"><span class=\"input-checkbox-faux\"><\/span>NEBNext rRNA Depletion Kit v2 (Human\/Mouse\/Rat)&nbsp;| <a href=\"https:\/\/international.neb.com\/E7400\" target=\"_blank\">Info<\/a><\/label><\/span><span class=\"oneChoice\"><input type=\"checkbox\" value=\"tfa_53\" class=\"calc-NEBNEXTMODULE calcval-E7850S\\u0020NEBNext\\u0020rRNA\\u0020Depletion\\u0020Kit\\u0020(Bacteria)\\n\" id=\"tfa_53\" name=\"tfa_53\" aria-labelledby=\"tfa_53-L\" data-tfa-labelledby=\"tfa_50-L tfa_53-L\" data-tfa-parent-id=\"tfa_50\"><label class=\"label postField\" id=\"tfa_53-L\" for=\"tfa_53\"><span class=\"input-checkbox-faux\"><\/span>NEBNext rRNA Depletion Kit (Bacteria)&nbsp;| <a href=\"https:\/\/international.neb.com\/E7850\" target=\"_blank\">Info<\/a><\/label><\/span><span class=\"oneChoice\"><input type=\"checkbox\" value=\"tfa_54\" class=\"calc-NEBNEXTMODULE calcval-E6421G\\u0020NEBNext\\u0020Single\\u0020Cell\/Low\\u0020Input\\u0020cDNA\\u0020Synthesis\\u0020&amp;\\u0020Amplification\\u0020Module\\n\" id=\"tfa_54\" name=\"tfa_54\" aria-labelledby=\"tfa_54-L\" data-tfa-labelledby=\"tfa_50-L tfa_54-L\" data-tfa-parent-id=\"tfa_50\"><label class=\"label postField\" id=\"tfa_54-L\" for=\"tfa_54\"><span class=\"input-checkbox-faux\"><\/span>NEBNext Single Cell\/Low Input cDNA Synthesis &amp; Amplification Module&nbsp;| <a href=\"https:\/\/international.neb.com\/E6421\" target=\"_blank\">Info<\/a><\/label><\/span><span class=\"oneChoice\"><input type=\"checkbox\" value=\"tfa_55\" class=\"calc-NEBNEXTMODULE calcval-E7490G\\u0020NEBNext\\u0020Poly(A)\\u0020mRNA\\u0020Magnetic\\u0020Isolation\\u0020Module\\n\" id=\"tfa_55\" name=\"tfa_55\" aria-labelledby=\"tfa_55-L\" data-tfa-labelledby=\"tfa_50-L tfa_55-L\" data-tfa-parent-id=\"tfa_50\"><label class=\"label postField\" id=\"tfa_55-L\" for=\"tfa_55\"><span class=\"input-checkbox-faux\"><\/span>NEBNext Poly(A) mRNA Magnetic Isolation Module&nbsp;| <a href=\"https:\/\/international.neb.com\/E7490\" target=\"_blank\">Info<\/a><\/label><\/span><span class=\"oneChoice\"><input type=\"checkbox\" value=\"tfa_56\" class=\"calc-NEBNEXTMODULE calcval-M0544G\\u0020NEBNext\\u0020Ultra\\u0020II\\u0020Q5\\u0020Master\\u0020Mix\\n\" id=\"tfa_56\" name=\"tfa_56\" aria-labelledby=\"tfa_56-L\" data-tfa-labelledby=\"tfa_50-L tfa_56-L\" data-tfa-parent-id=\"tfa_50\"><label class=\"label postField\" id=\"tfa_56-L\" for=\"tfa_56\"><span class=\"input-checkbox-faux\"><\/span>NEBNext Ultra II Q5 Master Mix&nbsp;| <a href=\"https:\/\/international.neb.com\/M0544\" target=\"_blank\">Info<\/a><\/label><\/span><span class=\"oneChoice\"><input type=\"checkbox\" value=\"tfa_57\" class=\"calc-NEBNEXTMODULE calcval-M0597G\\u0020NEBNext\\u0020Q5U\\u0020Master\\u0020Mix\\n\" id=\"tfa_57\" name=\"tfa_57\" aria-labelledby=\"tfa_57-L\" data-tfa-labelledby=\"tfa_50-L tfa_57-L\" data-tfa-parent-id=\"tfa_50\"><label class=\"label postField\" id=\"tfa_57-L\" for=\"tfa_57\"><span class=\"input-checkbox-faux\"><\/span>NEBNext Q5U Master Mix&nbsp;| <a href=\"https:\/\/international.neb.com\/M0597\" target=\"_blank\">Info<\/a><\/label><\/span><span class=\"oneChoice\"><input type=\"checkbox\" value=\"tfa_58\" class=\"calc-NEBNEXTMODULE calcval-E7360s\\u0020NEBNext\\u0020FFPE\\u0020DNA\\u0020Repair\\u0020v2\\u0020Module\\n\" id=\"tfa_58\" name=\"tfa_58\" aria-labelledby=\"tfa_58-L\" data-tfa-labelledby=\"tfa_50-L tfa_58-L\" data-tfa-parent-id=\"tfa_50\"><label class=\"label postField\" id=\"tfa_58-L\" for=\"tfa_58\"><span class=\"input-checkbox-faux\"><\/span>NEBNext FFPE DNA Repair v2 Module&nbsp;| <a href=\"https:\/\/international.neb.com\/M6630\" target=\"_blank\"><\/a><a href=\"https:\/\/international.neb.com\/E7360\" target=\"_blank\">Info<\/a><\/label><\/span><span class=\"oneChoice\"><input type=\"checkbox\" value=\"tfa_59\" class=\"calc-NEBNEXTMODULE calcval-E7335G\\u0020NEBNext\\u0020Multiplex\\u0020Oligos\\u0020for\\u0020Illumina\\n\" id=\"tfa_59\" name=\"tfa_59\" aria-labelledby=\"tfa_59-L\" data-tfa-labelledby=\"tfa_50-L tfa_59-L\" data-tfa-parent-id=\"tfa_50\"><label class=\"label postField\" id=\"tfa_59-L\" for=\"tfa_59\"><span class=\"input-checkbox-faux\"><\/span>NEBNext Multiplex Oligos for Illumina&nbsp;| <a href=\"https:\/\/international.neb.com\/E7335\" target=\"_blank\">Info<\/a><\/label><\/span><span class=\"oneChoice\"><input type=\"checkbox\" value=\"tfa_60\" class=\"calc-NEBNEXTMODULE calcval-E7630S\\u0020NEBNext\\u0020Library\\u0020Quant\\u0020Kit\\u0020for\\u0020Illumina\\n\" id=\"tfa_60\" name=\"tfa_60\" aria-labelledby=\"tfa_60-L\" data-tfa-labelledby=\"tfa_50-L tfa_60-L\" data-tfa-parent-id=\"tfa_50\"><label class=\"label postField\" id=\"tfa_60-L\" for=\"tfa_60\"><span class=\"input-checkbox-faux\"><\/span>NEBNext Library Quant Kit for Illumina&nbsp;| <a href=\"https:\/\/international.neb.com\/E7630\" target=\"_blank\">Info<\/a><\/label><\/span><span class=\"oneChoice\"><input type=\"checkbox\" value=\"tfa_126\" class=\"calc-NEBNEXTMODULE calcval-E8020G\\u0020NEBNext\\u0020Enzymatic\\u0020Methyl-seq\\u0020v2\\u0020Conversion\\u0020Module\\n\" id=\"tfa_126\" name=\"tfa_126\" aria-labelledby=\"tfa_126-L\" data-tfa-labelledby=\"tfa_50-L tfa_126-L\" data-tfa-parent-id=\"tfa_50\"><label class=\"label postField\" id=\"tfa_126-L\" for=\"tfa_126\"><span class=\"input-checkbox-faux\"><\/span>NEBNext Enzymatic Methyl-seq v2 Conversion Module | <a href=\"https:\/\/www.neb.com\/E8020\">Info<\/a><\/label><\/span><span class=\"oneChoice\"><input type=\"checkbox\" value=\"tfa_115\" class=\"calc-NEBNEXTMODULE calcval-M7634G\\u0020NEBNext\\u0020Ultrashear\\n\" id=\"tfa_115\" name=\"tfa_115\" aria-labelledby=\"tfa_115-L\" data-tfa-labelledby=\"tfa_50-L tfa_115-L\" data-tfa-parent-id=\"tfa_50\"><label class=\"label postField\" id=\"tfa_115-L\" for=\"tfa_115\"><span class=\"input-checkbox-faux\"><\/span>NEBNext UltraShear | <a href=\"https:\/\/www.neb.com\/m7634\" target=\"_blank\">Info<\/a><\/label><\/span><span class=\"oneChoice\"><input type=\"checkbox\" value=\"tfa_121\" class=\"calc-NEBNEXTMODULE calcval-E3365G\\u0020NEBNext\\u0020Enzymatic\\u00205hmC-seq\\u0020Conversion\\u0020Module\\n\" id=\"tfa_121\" name=\"tfa_121\" aria-labelledby=\"tfa_121-L\" data-tfa-labelledby=\"tfa_50-L tfa_121-L\" data-tfa-parent-id=\"tfa_50\"><label class=\"label postField\" id=\"tfa_121-L\" for=\"tfa_121\"><span class=\"input-checkbox-faux\"><\/span>NEBNext Enzymatic 5hmC-seq Conversion Module | <a target=\"_blank\" href=\"https:\/\/www.neb.com\/en\/E3365\">Info<\/a><\/label><\/span><span class=\"oneChoice\"><input type=\"checkbox\" value=\"tfa_124\" class=\"calc-NEBNEXTMODULE calcval-E9720G\\u0020NEBNext\\u0020Circularization\\u0020Module\\u0020for\\u0020MGI\\n\" id=\"tfa_124\" name=\"tfa_124\" aria-labelledby=\"tfa_124-L\" data-tfa-labelledby=\"tfa_50-L tfa_124-L\" data-tfa-parent-id=\"tfa_50\"><label class=\"label postField\" id=\"tfa_124-L\" for=\"tfa_124\"><span class=\"input-checkbox-faux\"><\/span>NEBNext Circularization Module for MGI | <a target=\"_blank\" href=\"https:\/\/www.neb.com\/en\/E9720\">Info<\/a><\/label><\/span><\/span><\/div>\n<\/div>\n<div class=\"oneField field-container-D  labelsRemoved  \" id=\"tfa_61-D\" role=\"group\" aria-labelledby=\"tfa_61-L\" data-tfa-labelledby=\"-L tfa_61-L\"><div class=\"inputWrapper\"><span id=\"tfa_61\" class=\"choices vertical \"><span class=\"oneChoice\"><input type=\"checkbox\" value=\"tfa_62\" class=\"\" id=\"tfa_62\" name=\"tfa_62\" data-conditionals=\"#tfa_63\" aria-labelledby=\"tfa_62-L\" data-tfa-labelledby=\"tfa_61-L tfa_62-L\" data-tfa-parent-id=\"tfa_61\"><label class=\"label postField\" id=\"tfa_62-L\" for=\"tfa_62\"><span class=\"input-checkbox-faux\"><\/span>Sample not listed<\/label><\/span><\/span><\/div><\/div>\n<div class=\"oneField field-container-D    \" id=\"tfa_63-D\">\n<label id=\"tfa_63-L\" class=\"label preField reqMark\" for=\"tfa_63\">Please send me the following samples:<\/label><br><div class=\"inputWrapper\"><input aria-required=\"true\" type=\"text\" id=\"tfa_63\" name=\"tfa_63\" value=\"\" data-condition=\"`#tfa_62`\" title=\"Please send me the following samples:\" class=\"calc-ANDERE required\"><\/div>\n<\/div>\n<div class=\"oneField field-container-D    \" id=\"tfa_64-D\" role=\"group\" aria-labelledby=\"tfa_64-L\" data-tfa-labelledby=\"-L tfa_64-L\">\n<label id=\"tfa_64-L\" class=\"label preField \" data-tfa-check-label-for=\"tfa_64\"><div>Monarch DNA &amp; RNA Kits perfectly complement the NEBNext products<\/div><\/label><br><div class=\"inputWrapper\"><span id=\"tfa_64\" class=\"choices vertical \"><span class=\"oneChoice\"><input type=\"checkbox\" value=\"tfa_66\" class=\"calc-MONARCH calcval-T2010G\\u0020Monarch\\u0020Total\\u0020RNA\\u0020Miniprep\\u0020Kit\\u0020(RNA\\u0020Isolation)\\n\" id=\"tfa_66\" name=\"tfa_66\" aria-labelledby=\"tfa_66-L\" data-tfa-labelledby=\"tfa_64-L tfa_66-L\" data-tfa-parent-id=\"tfa_64\"><label class=\"label postField\" id=\"tfa_66-L\" for=\"tfa_66\"><span class=\"input-checkbox-faux\"><\/span>Monarch Total RNA Miniprep Kit (RNA Isolation)<\/label><\/span><span class=\"oneChoice\"><input type=\"checkbox\" value=\"tfa_112\" class=\"calc-MONARCH calcval-T3050G\\u0020Monarch\\u0020HMW\\u0020DNA\\u0020Extraction\\u0020Kit\\u0020for\\u0020Cells\\u0020Blood\\u0020(A-Sample!!!)\\n\" id=\"tfa_112\" name=\"tfa_112\" aria-labelledby=\"tfa_112-L\" data-tfa-labelledby=\"tfa_64-L tfa_112-L\" data-tfa-parent-id=\"tfa_64\"><label class=\"label postField\" id=\"tfa_112-L\" for=\"tfa_112\"><span class=\"input-checkbox-faux\"><\/span>Monarch HMW DNA Extraction Kit for Cells &amp; Blood<\/label><\/span><span class=\"oneChoice\"><input type=\"checkbox\" value=\"tfa_113\" class=\"calc-MONARCH calcval-T3060G\\u0020Monarch\\u0020HMW\\u0020DNA\\u0020Extraction\\u0020Kit\\u0020for\\u0020Tissue\\u0020(A-Sample!!!)\\n\" id=\"tfa_113\" name=\"tfa_113\" aria-labelledby=\"tfa_113-L\" data-tfa-labelledby=\"tfa_64-L tfa_113-L\" data-tfa-parent-id=\"tfa_64\"><label class=\"label postField\" id=\"tfa_113-L\" for=\"tfa_113\"><span class=\"input-checkbox-faux\"><\/span>Monarch HMW DNA Extraction Kit for Tissue<\/label><\/span><span class=\"oneChoice\"><input type=\"checkbox\" value=\"tfa_128\" class=\"calc-MONARCH calcval-T1130G\\u0020Monarch\\u0020Spin\\u0020PCR\\u0020&amp;\\u0020Kit\\u0020DNA\\u0020Cleanup\\u0020Kit\\u0020(A-Sample!!!)\\n\" id=\"tfa_128\" name=\"tfa_128\" aria-labelledby=\"tfa_128-L\" data-tfa-labelledby=\"tfa_64-L tfa_128-L\" data-tfa-parent-id=\"tfa_64\"><label class=\"label postField\" id=\"tfa_128-L\" for=\"tfa_128\"><span class=\"input-checkbox-faux\"><\/span>Monarch Spin PCR &amp; DNA Cleanup Kit (5ug)<\/label><\/span><\/span><\/div>\n<\/div>\n<div class=\"oneField field-container-D    \" id=\"tfa_68-D\" role=\"group\" aria-labelledby=\"tfa_68-L\" data-tfa-labelledby=\"-L tfa_68-L\">\n<label id=\"tfa_68-L\" class=\"label preField \" data-tfa-check-label-for=\"tfa_68\">What sequencing platform(s) do you use?<\/label><br><div class=\"inputWrapper\"><span id=\"tfa_68\" class=\"choices vertical \"><span class=\"oneChoice\"><input type=\"checkbox\" value=\"tfa_69\" class=\"\" id=\"tfa_69\" name=\"tfa_69\" aria-labelledby=\"tfa_69-L\" data-tfa-labelledby=\"tfa_68-L tfa_69-L\" data-tfa-parent-id=\"tfa_68\"><label class=\"label postField\" id=\"tfa_69-L\" for=\"tfa_69\"><span class=\"input-checkbox-faux\"><\/span>Illumina<\/label><\/span><span class=\"oneChoice\"><input type=\"checkbox\" value=\"tfa_70\" class=\"\" id=\"tfa_70\" name=\"tfa_70\" aria-labelledby=\"tfa_70-L\" data-tfa-labelledby=\"tfa_68-L tfa_70-L\" data-tfa-parent-id=\"tfa_68\"><label class=\"label postField\" id=\"tfa_70-L\" for=\"tfa_70\"><span class=\"input-checkbox-faux\"><\/span>Oxford Nanopore<\/label><\/span><span class=\"oneChoice\"><input type=\"checkbox\" value=\"tfa_71\" class=\"\" id=\"tfa_71\" name=\"tfa_71\" data-conditionals=\"#tfa_72\" aria-labelledby=\"tfa_71-L\" data-tfa-labelledby=\"tfa_68-L tfa_71-L\" data-tfa-parent-id=\"tfa_68\"><label class=\"label postField\" id=\"tfa_71-L\" for=\"tfa_71\"><span class=\"input-checkbox-faux\"><\/span><div>Other platform \/ application<\/div><\/label><\/span><\/span><\/div>\n<\/div>\n<div class=\"oneField field-container-D    \" id=\"tfa_72-D\">\n<label id=\"tfa_72-L\" class=\"label preField reqMark\" for=\"tfa_72\">Platform \/ Application<\/label><br><div class=\"inputWrapper\"><input aria-required=\"true\" type=\"text\" id=\"tfa_72\" name=\"tfa_72\" value=\"\" data-condition=\"`#tfa_71`\" title=\"Platform \/ Application\" class=\"required\"><\/div>\n<\/div>\n<div class=\"htmlSection\" id=\"tfa_74\"><div class=\"htmlContent\" id=\"tfa_74-HTML\"><hr><\/div><\/div>\n<div class=\"htmlSection\" id=\"tfa_75\"><div class=\"htmlContent\" id=\"tfa_75-HTML\"><font color=\"#5c6b80\"><span style=\"font-size: 19.2px;\"><b>Your institutional address:<\/b><\/span><\/font><\/div><\/div>\n<div id=\"tfa_8\" class=\"section column group\"><table class=\"columnLayout\">\n<tr>\n<td><div class=\"oneField field-container-D    \" id=\"tfa_40-D\">\n<label id=\"tfa_40-L\" class=\"label preField reqMark\" for=\"tfa_40\">E-Mail<\/label><br><div class=\"inputWrapper\"><input aria-required=\"true\" type=\"text\" id=\"tfa_40\" name=\"tfa_40\" value=\"\" autocomplete=\"off\" title=\"E-Mail\" class=\"validate-email required\"><\/div>\n<\/div><\/td>\n<td><div class=\"oneField field-container-D     wf-acl-hidden\" id=\"tfa_42-D\">\n<label id=\"tfa_42-L\" class=\"label preField \" for=\"tfa_42\">Platzhalter<\/label><br><div class=\"inputWrapper\"><input type=\"text\" id=\"tfa_42\" name=\"tfa_42\" value=\"\" title=\"Platzhalter\" class=\"\"><\/div>\n<\/div><\/td>\n<\/tr>\n<tr>\n<td><div class=\"oneField field-container-D    \" id=\"tfa_9-D\">\n<label id=\"tfa_9-L\" class=\"label preField reqMark\" for=\"tfa_9\">Salutation<\/label><br><div class=\"inputWrapper\"><select aria-required=\"true\" id=\"tfa_9\" name=\"tfa_9\" title=\"Salutation\" class=\"required\"><option value=\"\">Please select...<\/option>\n<option value=\"tfa_10\" id=\"tfa_10\" class=\"\">Ms.<\/option>\n<option value=\"tfa_78\" id=\"tfa_78\" class=\"\">Mrs.<\/option>\n<option value=\"tfa_11\" id=\"tfa_11\" class=\"\">Mr.<\/option>\n<option value=\"tfa_114\" id=\"tfa_114\" class=\"\">Mx.<\/option><\/select><\/div>\n<\/div><\/td>\n<td><div class=\"oneField field-container-D    \" id=\"tfa_15-D\">\n<label id=\"tfa_15-L\" class=\"label preField \" for=\"tfa_15\">Title<\/label><br><div class=\"inputWrapper\"><select id=\"tfa_15\" name=\"tfa_15\" title=\"Title\" class=\"\"><option value=\"\">Please select...<\/option>\n<option value=\"tfa_16\" id=\"tfa_16\" class=\"\">Dr.<\/option>\n<option value=\"tfa_17\" id=\"tfa_17\" class=\"\">Professor<\/option><\/select><\/div>\n<\/div><\/td>\n<\/tr>\n<tr>\n<td><div class=\"oneField field-container-D    \" id=\"tfa_18-D\">\n<label id=\"tfa_18-L\" class=\"label preField reqMark\" for=\"tfa_18\">Last name<\/label><br><div class=\"inputWrapper\"><input aria-required=\"true\" type=\"text\" id=\"tfa_18\" name=\"tfa_18\" value=\"\" autocomplete=\"off\" title=\"Last name\" class=\"required\"><\/div>\n<\/div><\/td>\n<td><div class=\"oneField field-container-D    \" id=\"tfa_20-D\">\n<label id=\"tfa_20-L\" class=\"label preField \" for=\"tfa_20\">First name<\/label><br><div class=\"inputWrapper\"><input type=\"text\" id=\"tfa_20\" name=\"tfa_20\" value=\"\" autocomplete=\"off\" title=\"First name\" class=\"\"><\/div>\n<\/div><\/td>\n<\/tr>\n<tr>\n<td><div class=\"oneField field-container-D    \" id=\"tfa_22-D\">\n<label id=\"tfa_22-L\" class=\"label preField reqMark\" for=\"tfa_22\">Company \/ Organization<\/label><br><div class=\"inputWrapper\"><input aria-required=\"true\" type=\"text\" id=\"tfa_22\" name=\"tfa_22\" value=\"\" title=\"Company \/ Organization\" class=\"required\"><\/div>\n<\/div><\/td>\n<td><div class=\"oneField field-container-D    \" id=\"tfa_24-D\">\n<label id=\"tfa_24-L\" class=\"label preField \" for=\"tfa_24\">Department \/ Institute \/ Workgroup<\/label><br><div class=\"inputWrapper\"><input type=\"text\" id=\"tfa_24\" name=\"tfa_24\" value=\"\" title=\"Department \/ Institute \/ Workgroup\" class=\"\"><\/div>\n<\/div><\/td>\n<\/tr>\n<tr>\n<td><div class=\"oneField field-container-D    \" id=\"tfa_26-D\">\n<label id=\"tfa_26-L\" class=\"label preField \" for=\"tfa_26\">Building \/ Floor \/ Room Number<\/label><br><div class=\"inputWrapper\"><input type=\"text\" id=\"tfa_26\" name=\"tfa_26\" value=\"\" title=\"Building \/ Floor \/ Room Number\" class=\"\"><\/div>\n<\/div><\/td>\n<td><div class=\"oneField field-container-D    \" id=\"tfa_130-D\">\n<label id=\"tfa_130-L\" class=\"label preField reqMark\" for=\"tfa_130\">Street<\/label><br><div class=\"inputWrapper\"><input aria-required=\"true\" type=\"text\" id=\"tfa_130\" name=\"tfa_130\" value=\"\" title=\"Street\" class=\"required\"><\/div>\n<\/div><\/td>\n<\/tr>\n<tr>\n<td><div class=\"oneField field-container-D    \" id=\"tfa_28-D\">\n<label id=\"tfa_28-L\" class=\"label preField reqMark\" for=\"tfa_28\">Number<\/label><br><div class=\"inputWrapper\"><input aria-required=\"true\" type=\"text\" id=\"tfa_28\" name=\"tfa_28\" value=\"\" title=\"Number\" class=\"required\"><\/div>\n<\/div><\/td>\n<td><div class=\"oneField field-container-D    \" id=\"tfa_30-D\">\n<label id=\"tfa_30-L\" class=\"label preField reqMark\" for=\"tfa_30\">ZIP \/ Postal Code<\/label><br><div class=\"inputWrapper\"><input aria-required=\"true\" type=\"text\" id=\"tfa_30\" name=\"tfa_30\" value=\"\" title=\"ZIP \/ Postal Code\" class=\"required\"><\/div>\n<\/div><\/td>\n<\/tr>\n<tr>\n<td><div class=\"oneField field-container-D    \" id=\"tfa_32-D\">\n<label id=\"tfa_32-L\" class=\"label preField reqMark\" for=\"tfa_32\">City<\/label><br><div class=\"inputWrapper\"><input aria-required=\"true\" type=\"text\" id=\"tfa_32\" name=\"tfa_32\" value=\"\" title=\"City\" class=\"required\"><\/div>\n<\/div><\/td>\n<td><div class=\"oneField field-container-D    \" id=\"tfa_35-D\">\n<label id=\"tfa_35-L\" class=\"label preField reqMark\" for=\"tfa_35\">Country<\/label><br><div class=\"inputWrapper\"><select aria-required=\"true\" id=\"tfa_35\" name=\"tfa_35\" title=\"Country\" class=\"required\"><option value=\"\">Please select...<\/option>\n<option value=\"tfa_36\" id=\"tfa_36\" class=\"\">Germany<\/option>\n<option value=\"tfa_37\" id=\"tfa_37\" class=\"\">Austria<\/option>\n<option value=\"tfa_38\" id=\"tfa_38\" class=\"\">Other<\/option><\/select><\/div>\n<\/div><\/td>\n<\/tr>\n<tr><td><div class=\"oneField field-container-D    \" id=\"tfa_34-D\">\n<label id=\"tfa_34-L\" class=\"label preField \" for=\"tfa_34\">Phone<\/label><br><div class=\"inputWrapper\"><input type=\"text\" id=\"tfa_34\" name=\"tfa_34\" value=\"\" autocomplete=\"off\" title=\"Phone\" class=\"\"><\/div>\n<\/div><\/td><\/tr>\n<\/table><\/div>\n<div class=\"oneField field-container-D  labelsRemoved  \" id=\"tfa_4-D\" role=\"group\" aria-labelledby=\"tfa_4-L\" data-tfa-labelledby=\"-L tfa_4-L\"><div class=\"inputWrapper\"><span id=\"tfa_4\" class=\"choices vertical \"><span class=\"oneChoice\"><input type=\"checkbox\" value=\"tfa_5\" class=\"\" id=\"tfa_5\" name=\"tfa_5\" aria-labelledby=\"tfa_5-L\" data-tfa-labelledby=\"tfa_4-L tfa_5-L\" data-tfa-parent-id=\"tfa_4\"><label class=\"label postField\" id=\"tfa_5-L\" for=\"tfa_5\"><span class=\"input-checkbox-faux\"><\/span>I want to subscribe to the NEB eNews e-mail newsletter.<\/label><\/span><\/span><\/div><\/div>\n<div class=\"htmlSection\" id=\"tfa_6\"><div class=\"htmlContent\" id=\"tfa_6-HTML\">The NEB eNews newsletter appears monthly and on special occasions. 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Furthermore, for Illumina sequencing, accurate quantitation of libraries is critical to achieve optimal cluster densities, a requirement for optimal sequence output. qPCR is considered to be the most accurate&#8230;  <a class=\"excerpt-read-more\" href=\"https:\/\/www.neb-online.de\/en\/next-generation-sequencing\/nebnext-library-quant-kit-for-illumina\/\" title=\"Read NEBNext Library Quant Kit for Illumina\">Read more &raquo;<\/a><\/p>\n","protected":false},"author":18,"featured_media":40862,"parent":2170,"menu_order":0,"comment_status":"open","ping_status":"closed","template":"page-sidebar.php","meta":{"footnotes":""},"class_list":["post-4960","page","type-page","status-publish","has-post-thumbnail","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.1.1 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>NEBNext Library Quant Kit for Illumina - New England Biolabs GmbH<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.neb-online.de\/next-generation-sequencing\/nebnext-library-quant-kit-for-illumina\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"NEBNext Library Quant Kit for Illumina - New England Biolabs GmbH\" \/>\n<meta property=\"og:description\" content=\"[vc_row][vc_column][vc_basic_grid post_type=&#8221;post&#8221; max_items=&#8221;10&#8243; element_width=&#8221;12&#8243; gap=&#8221;15&#8243; item=&#8221;4123&#8243; taxonomies=&#8221;129&#8243; grid_id=&#8221;vc_gid:1702990565235-5cbd29f7-035a-4&#8243;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]Accurate quantitation of next-generation sequencing libraries is essential for maximizing data output and quality from each sequencing run. Furthermore, for Illumina sequencing, accurate quantitation of libraries is critical to achieve optimal cluster densities, a requirement for optimal sequence output. qPCR is considered to be the most accurate... 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