{"id":5679,"date":"2015-09-24T15:04:06","date_gmt":"2015-09-24T13:04:06","guid":{"rendered":"http:\/\/www.neb-online.de\/en\/?page_id=5679"},"modified":"2025-10-13T11:12:42","modified_gmt":"2025-10-13T09:12:42","slug":"nebnext-automation","status":"publish","type":"page","link":"https:\/\/www.neb-online.de\/en\/next-generation-sequencing\/nebnext-automation\/","title":{"rendered":"NEBNext Automation"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_column_text]<\/p>\n<h2><span style=\"color: #f58025;\"><strong>Scale up and automate your NGS projects using NEBNext Workflows, reagents and kits!<\/strong><\/span><\/h2>\n<p>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner][vc_column_inner][vc_column_text]Regardless whether you are in an academic Lab, a NGS Core Facility or in the industrial landscape \u2013 as sequencing instrument capacity is increasing, and the ever-growing range of sequencing applications is demanding expanded sample preparation throughputs, many NGS users are turning to automation.<\/p>\n<p>Manual preparation of large numbers of libraries can result in a sequencing workflow bottleneck, where the automation of workflows can provide a valuable solution to master these challenges and to maintain competitiveness and sustainability of any modern sequencing laboratory.[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_separator][vc_row_inner][vc_column_inner width=&#8221;1\/6&#8243; css=&#8221;.vc_custom_1597323363042{padding-top: 20px !important;}&#8221;][vc_single_image image=&#8221;24020&#8243; img_size=&#8221;full&#8221; alignment=&#8221;center&#8221;][\/vc_column_inner][vc_column_inner width=&#8221;5\/6&#8243;][vc_column_text]<\/p>\n<h3><strong>NGS Libary Prep with NEBNext<\/strong><\/h3>\n<p>Some crucial and decisive parameters for automation include a high scalability and fast workflows, reagent and process stability, reagent cost per reaction, and most importantly the quality of the sequencing data.<\/p>\n<p>No matter if small partial automation or complete \u201cwalk away\u201d solution \u2013 more and more laboratories choose to automate both DNA and RNA library prep workflows and benefit from the advantages of our NEBNext products:<\/p>\n<ul>\n<li>One central NEBNext Ultra II DNA workflow that is the core of the majority of the NEBNext workflows<\/li>\n<li>Modularity of workflows allows a maximum in flexibility with minimal effort during implementation of optional, additional modules (e.g. Ultra II RNA, Single Cell RNA Seq)<\/li>\n<li>Automation-friendly workflows with a minimum in cleanup steps and effective utilization of resources<\/li>\n<li>Reagent and process stability regardless of the input amount to guarantee consistent library quality and yield<\/li>\n<li>Reduced costs by minimizing the consumption of consumables for a low price per reaction<\/li>\n<li>Optimized volumes of reagents provided to allow a robust automation process<\/li>\n<li>Highly flexible as protocols are available for many different liquid handling automation platforms<\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_column_text]<span style=\"color: #f58025;\"><strong>Automation-friendly &#8220;by nature&#8221;:<\/strong><\/span><\/p>\n<p>You can use our regular and inexpensive NEBNext catalogue products for the automation of the NEBNext workflows \u2013 no expensive special formulations are required. However, if desired, we can certainly also offer a &#8220;customized solution&#8221;, that is tailored to your particular needs.[\/vc_column_text][vc_row_inner bwd_tbc4vd_show_weekday=&#8221;&#8221; css=&#8221;.vc_custom_1598020087601{margin-bottom: 40px !important;}&#8221;][vc_column_inner width=&#8221;1\/2&#8243; css=&#8221;.vc_custom_1597323363042{padding-top: 20px !important;}&#8221;]<div class=\"vbox\">\n\t\t\t\t<span class=\"vhead\">Advantages:<\/span>\n\t\t\t\t\t<ul class=\"vlist\">\n\t\t\t\t\t\t<li><span>One central NEBNext Ultra II DNA workflow that is the core of the majority of the NEBNext workflows<br \/><\/span><\/li><li><span>Modularity of NEBNext workflows allows for a simple automation process<br \/><\/span><\/li><li><span>Reagent and process stability regardless of the input amount<br \/><\/span><\/li><li><span>High scalability and flexibility<br \/><\/span><\/li><li><span>Robust reagents guarantee consistent library quality and yield<br \/><\/span><\/li><li><span>Reduced consumption of consumables for a low price per reaction<br \/><\/span><\/li><li><span>Optimized volumes of reagents provided to allow a robust automation process<\/span><\/li>\n\t\t\t\t\t<\/ul>\n\t\t\t<\/div>[\/vc_column_inner][vc_column_inner width=&#8221;1\/2&#8243; css=&#8221;.vc_custom_1599041223830{padding-top: 12px !important;}&#8221;]\n\t<table class=\"balloon\">\n\t<tbody>\n\t<tr>\n\t<td class=\"top_left\"><\/td>\n\t<td class=\"top\" colspan=\"3\"><\/td>\n\t<td class=\"top_right\"><\/td>\n\t<\/tr>\n\t<tr>\n\t<td class=\"left\"><\/td>\n\t<td class=\"content\" colspan=\"3\">\n\t<p style=\"text-align: center;\">\u201eNEB is currently the only company, which is offering really ``automation friendly`` library preparation kits. The volumes of components are calculated to cover for unavoidable deadvolumes, reactant volumes are in range of most automated platforms. The stability of the NEBNext chemistry allows a broad range of automation strategies.\u201c<\/p>\n\t<p style=\"text-align: center;\"><strong>Dr. J\u00fcrgen Zimmermann, Senior Engineer - Automation in GeneCore, EMBL Heidelberg, Deutschland<\/strong><\/p>\n\t<\/td>\n\t<td class=\"right\"><\/td>\n\t<\/tr>\n\t<tr>\n\t<td class=\"bottom_left\"><\/td>\n\t<td class=\"bottom\" style=\"width: 60px;\"><\/td>\n\t<td class=\"arrow\"><img decoding=\"async\" src=\"\/wp-content\/themes\/neb-online-theme\/library\/images\/stories\/arrow.png\" alt=\"\" \/><\/td>\n\t<td class=\"bottom\" style=\"width: 507px;\"><\/td>\n\t<td class=\"bottom_right\"><\/td>\n\t<\/tr>\n\t<\/tbody>\n\t<\/table>[\/vc_column_inner][\/vc_row_inner][vc_separator][vc_row_inner][vc_column_inner width=&#8221;1\/6&#8243;][vc_single_image image=&#8221;22370&#8243; img_size=&#8221;full&#8221; alignment=&#8221;center&#8221;][\/vc_column_inner][vc_column_inner width=&#8221;5\/6&#8243;][vc_column_text]<span style=\"color: #f58025;\"><strong>High flexibility as protocols are available for different liquid handling automation platform providers <\/strong><\/span><span style=\"color: #6d6e71;\"><strong>\u2013 excerpt from our project list<br \/>\n<\/strong><\/span>[\/vc_column_text][vc_column_text]<\/p>\n<table style=\"width: 760px;\" border=\"0\" cellpadding=\"5\">\n<tbody>\n<tr>\n<td valign=\"top\">\n<p>NEBNext reagents have been automated on various liquid handling automation platforms, including:<\/p>\n<ul>\n<li>Beckman Coulter<\/li>\n<li>Eppendorf<\/li>\n<li>Hamilton<\/li>\n<li>MGI<\/li>\n<li>PerkinElmer<\/li>\n<li>Tecan<\/li>\n<\/ul>\n<\/td>\n<td valign=\"top\">\n<p>Several applications have already been automated with NEBNext reagents, such as:<\/p>\n<ul>\n<li>DNA-Seq<\/li>\n<li>Directional RNA-Seq<\/li>\n<li>Enzymatic Methyl-Seq<\/li>\n<li>Target Enrichment<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_column_text css=&#8221;&#8221;]<strong>Fast implementation and turnaround with NEBNext reagents:<br \/>\n<\/strong>Many liquid handling automation platforms can already be purchased with validated NEBNext scripts directly from the robotics manufacturer. This way workflows can be established and adapted to your needs in a very short time.<\/p>\n<p>&nbsp;<\/p>\n<img loading=\"lazy\" decoding=\"async\" class=\"wp-image-49265 alignleft\" src=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2025\/06\/NEBNextAutomation_automationIcon_0624-1-300x212.png\" alt=\"\" width=\"107\" height=\"76\" srcset=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2025\/06\/NEBNextAutomation_automationIcon_0624-1-300x212.png 300w, https:\/\/www.neb-online.de\/wp-content\/uploads\/2025\/06\/NEBNextAutomation_automationIcon_0624-1.png 401w\" sizes=\"auto, (max-width: 107px) 100vw, 107px\" \/>\n<p style=\"text-align: left;\"><a href=\"https:\/\/www.neb.com\/en\/products\/next-generation-sequencing-library-preparation\/~\/link.aspx?_id=B2517BC8F98D4A299D42289AB028DF79&amp;_z=z\">NEBNext\u00ae Library Prep Kit<br \/>\nAutomation-compatibility Selection Chart<\/a><\/p>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row bwd_tbc4vd_show_weekday=&#8221;&#8221; css=&#8221;.vc_custom_1760346738259{border-top-width: 1px !important;border-right-width: 1px !important;border-bottom-width: 1px !important;border-left-width: 1px !important;padding-top: 40px !important;padding-right: 10px !important;padding-bottom: 20px !important;padding-left: 10px !important;border-left-style: solid !important;border-right-style: solid !important;border-top-style: solid !important;border-bottom-style: solid !important;border-radius: 1px !important;border-color: #e0e0e0 !important;}&#8221;][vc_column]<div id=\"shorten-45913\" class=\"shorten\" style=\"max-height:500px;\">[vc_row_inner bwd_tbc4vd_show_weekday=&#8221;&#8221;][vc_column_inner width=&#8221;1\/6&#8243;][vc_single_image image=&#8221;24046&#8243; img_size=&#8221;full&#8221; alignment=&#8221;center&#8221;][\/vc_column_inner][vc_column_inner width=&#8221;5\/6&#8243;][vc_column_text]<\/p>\n<h2><span style=\"color: #f58025;\">Please contact our automation specialists:<br \/>\nWe are happy to advise and assist 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#eb7e2d;border-width: 0;}\n    .columnLayout {table-layout: fixed;}\n  .supportInfo {padding:0 !important; border: 0 !important;}\n  #submit_button {padding: 8px 30px !important; font-weight: bold !important;}\n  input[type=\"checkbox\"] {min-width:20px !important; min-height:20px !important;}\n    input[type=\"radio\"] {min-width:20px !important; min-height:20px !important;}\n<\/style><\/div>\n<form method=\"post\" action=\"https:\/\/newenglandbiolabs.tfaforms.net\/api_v2\/rest\/workflow\/processor\" class=\"hintsBelow labelsAbove\" id=\"338408\">\n<div class=\"htmlSection\" id=\"tfa_46\"><div class=\"htmlContent\" id=\"tfa_46-HTML\">Fields marked&nbsp;<span style=\"color: red;\">*<\/span> are required<\/div><\/div>\n<div class=\"htmlSection\" id=\"tfa_70\"><div class=\"htmlContent\" id=\"tfa_70-HTML\"><span style=\"font-size:1.2em;font-weight:bold;color:#5c6b80;\"><br>I am interested in automation solutions for:<\/span><\/div><\/div>\n<div id=\"tfa_51\" class=\"section column group\"><table class=\"columnLayout\"><tr>\n<td><div class=\"oneField field-container-D    \" id=\"tfa_47-D\">\n<label id=\"tfa_47-L\" class=\"label preField \" for=\"tfa_47\">Workflow<\/label><br><div class=\"inputWrapper\"><select id=\"tfa_47\" name=\"tfa_47\" title=\"Workflow\" class=\"\"><option value=\"\">Please select...<\/option>\n<option value=\"tfa_48\" id=\"tfa_48\" class=\"\">DNA Sequencing<\/option>\n<option value=\"tfa_49\" id=\"tfa_49\" class=\"\">DNA Targeted Sequencing<\/option>\n<option value=\"tfa_50\" id=\"tfa_50\" class=\"\">RNA Sequencing<\/option>\n<option value=\"tfa_60\" id=\"tfa_60\" class=\"\">Other<\/option><\/select><\/div>\n<\/div><\/td>\n<td><div class=\"oneField field-container-D    \" id=\"tfa_56-D\">\n<label id=\"tfa_56-L\" class=\"label preField \" for=\"tfa_56\">Automation platform provider<\/label><br><div class=\"inputWrapper\"><select id=\"tfa_56\" name=\"tfa_56\" title=\"Automation platform provider\" class=\"\"><option value=\"\">Please select...<\/option>\n<option value=\"tfa_61\" id=\"tfa_61\" class=\"\">Beckman Coulter<\/option>\n<option value=\"tfa_62\" id=\"tfa_62\" class=\"\">Eppendorf<\/option>\n<option value=\"tfa_63\" id=\"tfa_63\" class=\"\">Hamilton<\/option>\n<option value=\"tfa_64\" id=\"tfa_64\" class=\"\">Perkin Elmer<\/option>\n<option value=\"tfa_65\" id=\"tfa_65\" class=\"\">Tecan<\/option>\n<option value=\"tfa_66\" id=\"tfa_66\" class=\"\">Other<\/option><\/select><\/div>\n<\/div><\/td>\n<\/tr><\/table><\/div>\n<div class=\"htmlSection\" id=\"tfa_44\"><div class=\"htmlContent\" id=\"tfa_44-HTML\"><hr><\/div><\/div>\n<div class=\"htmlSection\" id=\"tfa_72\"><div class=\"htmlContent\" id=\"tfa_72-HTML\"><font color=\"#5c6b80\"><span style=\"font-size: 19.2px;\"><b>Message:<\/b><\/span><\/font><\/div><\/div>\n<div class=\"oneField field-container-D  labelsRemoved  \" id=\"tfa_73-D\"><div class=\"inputWrapper\"><textarea id=\"tfa_73\" name=\"tfa_73\" title=\"Nachricht\" class=\"\"><\/textarea><\/div><\/div>\n<div class=\"htmlSection\" id=\"tfa_68\"><div class=\"htmlContent\" id=\"tfa_68-HTML\"><hr><\/div><\/div>\n<div class=\"htmlSection\" id=\"tfa_43\"><div class=\"htmlContent\" id=\"tfa_43-HTML\"><span style=\"font-size:1.2em;font-weight:bold;color:#5c6b80;\">My business\/ institutional address:<\/span><\/div><\/div>\n<div id=\"tfa_8\" class=\"section column group\"><table class=\"columnLayout\">\n<tr>\n<td><div class=\"oneField field-container-D    \" id=\"tfa_40-D\">\n<label id=\"tfa_40-L\" class=\"label preField reqMark\" for=\"tfa_40\">E-Mail<\/label><br><div class=\"inputWrapper\"><input aria-required=\"true\" type=\"text\" id=\"tfa_40\" name=\"tfa_40\" value=\"\" autocomplete=\"off\" title=\"E-Mail\" class=\"validate-email required\"><\/div>\n<\/div><\/td>\n<td><div class=\"oneField field-container-D     wf-acl-hidden\" id=\"tfa_42-D\">\n<label id=\"tfa_42-L\" class=\"label preField \" for=\"tfa_42\">Platzhalter<\/label><br><div 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title=\"Title\" class=\"\"><option value=\"\">Please select...<\/option>\n<option value=\"tfa_16\" id=\"tfa_16\" class=\"\">Dr.<\/option>\n<option value=\"tfa_17\" id=\"tfa_17\" class=\"\">Professor<\/option><\/select><\/div>\n<\/div><\/td>\n<\/tr>\n<tr>\n<td><div class=\"oneField field-container-D    \" id=\"tfa_18-D\">\n<label id=\"tfa_18-L\" class=\"label preField reqMark\" for=\"tfa_18\">Last name<\/label><br><div class=\"inputWrapper\"><input aria-required=\"true\" type=\"text\" id=\"tfa_18\" name=\"tfa_18\" value=\"\" autocomplete=\"off\" title=\"Last name\" class=\"required\"><\/div>\n<\/div><\/td>\n<td><div class=\"oneField field-container-D    \" id=\"tfa_20-D\">\n<label id=\"tfa_20-L\" class=\"label preField \" for=\"tfa_20\">First name<\/label><br><div class=\"inputWrapper\"><input type=\"text\" id=\"tfa_20\" name=\"tfa_20\" value=\"\" autocomplete=\"off\" title=\"First name\" class=\"\"><\/div>\n<\/div><\/td>\n<\/tr>\n<tr>\n<td><div class=\"oneField field-container-D    \" 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css=&#8221;.vc_custom_1597325097520{margin-bottom: 0px !important;border-bottom-width: 0px !important;padding-bottom: 0px !important;}&#8221;][vc_column][vc_row_inner bwd_tbc4vd_show_weekday=&#8221;&#8221; disable_element=&#8221;yes&#8221;][vc_column_inner][vc_column_text css=&#8221;.vc_custom_1603894206339{margin-bottom: 0px !important;border-bottom-width: 0px !important;padding-bottom: 0px !important;}&#8221;]<\/p>\n<h3><span style=\"color: #f58025;\"><strong>Selected examples of successfully implemented automation projects:<\/strong><\/span><\/h3>\n<p>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner bwd_tbc4vd_show_weekday=&#8221;&#8221; disable_element=&#8221;yes&#8221; css=&#8221;.vc_custom_1673350064409{margin-top: 0px !important;border-top-width: 0px !important;padding-top: 0px !important;}&#8221;][vc_column_inner width=&#8221;1\/2&#8243; css=&#8221;.vc_custom_1597325139474{margin-top: 0px !important;border-top-width: 0px !important;padding-top: 10px !important;}&#8221;][vc_column_text css=&#8221;.vc_custom_1597323227799{margin-bottom: 0px !important;border-bottom-width: 0px !important;padding-bottom: 10px !important;}&#8221;]<strong>Eppendorf:<\/strong><br \/>\nAutomated library preparation using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina on the epMotion 5075t[\/vc_column_text]<a class=\"btn btn-standard form-btn\" target=\"_blank\" href=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2020\/03\/NEBNext_TechNote_Eppendorf_DirectRNAII.pdf\" style=\"margin-right:auto;\">PDF Download<\/a>[\/vc_column_inner][vc_column_inner width=&#8221;1\/2&#8243; css=&#8221;.vc_custom_1597325131483{margin-top: 0px !important;border-top-width: 0px !important;padding-top: 10px !important;}&#8221;][vc_column_text css=&#8221;.vc_custom_1598020140665{margin-bottom: 0px !important;border-bottom-width: 0px !important;padding-bottom: 10px !important;}&#8221;]<strong>Tecan:<\/strong><br \/>\nAutomated NEBNext Ultra II DNA Library Preparation for Illumina<br \/>\n<span style=\"color: #ffffff;\">.<\/span>[\/vc_column_text]<a class=\"btn btn-standard form-btn\" target=\"_blank\" href=\"https:\/\/www.google.de\/url?sa=t&amp;rct=j&amp;q=&amp;esrc=s&amp;source=web&amp;cd=1&amp;ved=0ahUKEwj9rO_vto7PAhWFPBQKHUS4ChYQFggyMAA&amp;url=http%3A%2F%2Fww3.tecan.com%2Fplatform%2Fapps%2Fdatainterface%2Fdownloadctrl.asp%3Fodl%3D1%26file%3D%2Fmandant%2Ffiles%2Fdoc%2F711%2FAN_Automated_NEBNext_Ultra_II_DNA_Library_Preparation_for_Illumina_399667_V1-0.pdf%26title%3DAutomated%2520NEBNext%25C2%25AE%2520ULTRA%2520II%2520DNA%2520LIBRARY%2520Preparation%2520for%2520Illumina%25C2%25AE&amp;usg=AFQjCNHmpuVI1nQ0YITNLJuVPpa5B-3NhA&amp;bvm=bv.132479545,d.d24\" style=\"margin-right:auto;\">PDF Download<\/a>[\/vc_column_inner][\/vc_row_inner][vc_empty_space][vc_row_inner][vc_column_inner width=&#8221;1\/6&#8243;][vc_single_image image=&#8221;24069&#8243; img_size=&#8221;full&#8221; alignment=&#8221;center&#8221;][\/vc_column_inner][vc_column_inner width=&#8221;5\/6&#8243;][vc_column_text]<\/p>\n<h2><span style=\"color: #f58025;\">Your preferred liquid handling automation platform or robotics manufacturer is not listed? Then please contact our automation specialists \u2013 we will be happy to advise and assist you with your automation project!<\/span><\/h2>\n<p>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_column_text] Scale up and automate your NGS projects using NEBNext Workflows, reagents and kits! [\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner][vc_column_inner][vc_column_text]Regardless whether you are in an academic Lab, a NGS Core Facility or in the industrial landscape \u2013 as sequencing instrument capacity is increasing, and the ever-growing range of sequencing applications is demanding expanded sample preparation throughputs, many NGS users are&#8230;  <a class=\"excerpt-read-more\" href=\"https:\/\/www.neb-online.de\/en\/next-generation-sequencing\/nebnext-automation\/\" title=\"Read NEBNext Automation\">Read more &raquo;<\/a><\/p>\n","protected":false},"author":18,"featured_media":24085,"parent":2170,"menu_order":0,"comment_status":"open","ping_status":"closed","template":"page-sidebar.php","meta":{"footnotes":""},"class_list":["post-5679","page","type-page","status-publish","has-post-thumbnail","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.1.1 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>NEBNext Automation - New England Biolabs GmbH<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.neb-online.de\/en\/next-generation-sequencing\/nebnext-automation\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"NEBNext Automation - New England Biolabs GmbH\" \/>\n<meta property=\"og:description\" content=\"[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_column_text] Scale up and automate your NGS projects using NEBNext Workflows, reagents and kits! [\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner][vc_column_inner][vc_column_text]Regardless whether you are in an academic Lab, a NGS Core Facility or in the industrial landscape \u2013 as sequencing instrument capacity is increasing, and the ever-growing range of sequencing applications is demanding expanded sample preparation throughputs, many NGS users are... 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