{"id":6407,"date":"2015-09-21T16:25:19","date_gmt":"2015-09-21T14:25:19","guid":{"rendered":"http:\/\/www.neb-online.de\/en\/?page_id=6407"},"modified":"2023-07-18T13:48:20","modified_gmt":"2023-07-18T11:48:20","slug":"neb-tools","status":"publish","type":"page","link":"https:\/\/www.neb-online.de\/en\/technical-resources-neb\/neb-tools\/","title":{"rendered":"NEB Interactive Tools"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row disable_element=&#8221;yes&#8221;][vc_column el_class=&#8221;tools-slider&#8221;][vc_images_carousel images=&#8221;6991,2007,2008,2015,2017,2018,2006,2013,2014,2016,2009,2053,2011,2012&#8243; img_size=&#8221;full&#8221; onclick=&#8221;custom_link&#8221; custom_links_target=&#8221;_blank&#8221; slides_per_view=&#8221;8&#8243; custom_links=&#8221;http:\/\/nebnextselector.neb.com,http:\/\/nc2.neb.com\/NEBcutter2\/,https:\/\/www.neb.com\/tools-and-resources\/interactive-tools\/double-digest-finder,http:\/\/tmcalculator.neb.com\/,http:\/\/nebiocalculator.neb.com\/,http:\/\/nebuilder.neb.com\/,http:\/\/nebcloner.neb.com\/,http:\/\/rebase.neb.com\/rebase\/,https:\/\/www.neb.com\/en\/tools-and-resources\/interactive-tools\/dna-sequences-and-maps-tool,http:\/\/nebasechanger.neb.com\/,https:\/\/www.neb.com\/tools-and-resources\/interactive-tools\/enzyme-finder,https:\/\/goldengate.neb.com\/editor,https:\/\/www.neb.com\/tools-and-resources\/interactive-tools\/pcr-selection-tool,http:\/\/polbase.neb.com\/&#8221; el_class=&#8221;tools-slider-8items&#8221;][vc_images_carousel images=&#8221;6991,2007,2008,2015,2017,2018,2006,2013,2014,2016,2009,2053,2011,2012&#8243; img_size=&#8221;full&#8221; onclick=&#8221;custom_link&#8221; custom_links_target=&#8221;_blank&#8221; slides_per_view=&#8221;4&#8243; custom_links=&#8221;http:\/\/nebnextselector.neb.com,http:\/\/nc2.neb.com\/NEBcutter2\/,https:\/\/www.neb.com\/tools-and-resources\/interactive-tools\/double-digest-finder,http:\/\/tmcalculator.neb.com\/,http:\/\/nebiocalculator.neb.com\/,http:\/\/nebuilder.neb.com\/,http:\/\/nebcloner.neb.com\/,http:\/\/rebase.neb.com\/rebase\/,https:\/\/www.neb.com\/en\/tools-and-resources\/interactive-tools\/dna-sequences-and-maps-tool,http:\/\/nebasechanger.neb.com\/,https:\/\/www.neb.com\/tools-and-resources\/interactive-tools\/enzyme-finder,https:\/\/goldengate.neb.com\/editor,https:\/\/www.neb.com\/tools-and-resources\/interactive-tools\/pcr-selection-tool,http:\/\/polbase.neb.com\/&#8221; el_class=&#8221;tools-slider-4items&#8221;][\/vc_column][\/vc_row][vc_row bwd_tbc4vd_show_weekday=&#8221;&#8221; css=&#8221;.vc_custom_1689680889623{margin-right: -1px !important;margin-left: -1px !important;border-top-width: 2px !important;border-right-width: 2px !important;border-bottom-width: 2px !important;border-left-width: 2px !important;padding-top: 10px !important;padding-right: 10px !important;padding-bottom: 10px !important;padding-left: 10px !important;border-left-color: #e0e0e0 !important;border-left-style: solid !important;border-right-color: #e0e0e0 !important;border-right-style: solid !important;border-top-color: #e0e0e0 !important;border-top-style: solid !important;border-bottom-color: #e0e0e0 !important;border-bottom-style: solid !important;border-radius: 1px !important;}&#8221;][vc_column width=&#8221;2\/3&#8243;][vc_column_text]<\/p>\n<h2><span style=\"color: #f58025;\">NEB Interactive Tools<\/span><\/h2>\n<ul>\n<li>Do you need advice choosing the right reaction buffer for your double digests?<\/li>\n<li>Or do you plan a complex DNA assembly and you need a tool for your primer design?<\/li>\n<\/ul>\n<p><strong>We have numerous interactive online tools for these and other questions in your daily lab work.<\/strong>[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/3&#8243;][vc_row_inner][vc_column_inner][vc_column_text]<span style=\"font-size: 20px; font-weight: bold;\">NEB Tools also available as mobile app!<\/span>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner][vc_column_inner width=&#8221;1\/2&#8243;][vc_single_image image=&#8221;6471&#8243; img_size=&#8221;full&#8221; onclick=&#8221;custom_link&#8221; img_link_target=&#8221;_blank&#8221; link=&#8221;http:\/\/itunes.apple.com\/us\/app\/neb-tools\/id350346827?mt=8&amp;ign-mpt=uo=6&#8243;][\/vc_column_inner][vc_column_inner width=&#8221;1\/2&#8243;][vc_single_image image=&#8221;6474&#8243; img_size=&#8221;full&#8221; onclick=&#8221;custom_link&#8221; img_link_target=&#8221;_blank&#8221; link=&#8221;https:\/\/play.google.com\/store\/apps\/details?id=com.neb.nebtools&#8221;][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row bwd_tbc4vd_show_weekday=&#8221;&#8221;][vc_column width=&#8221;2\/3&#8243;][vc_column_text]<\/p>\n<h2>NEB AR app with the Catalog &amp; Technical Reference<\/h2>\n<p>The NEB Catalog &amp; Technical Reference comes to life with the NEB AR App \u2013 just download at the <span style=\"color: #f58025;\"><a style=\"color: #f58025;\" href=\"https:\/\/apps.apple.com\/us\/app\/neb-ar\/id1451313043\" target=\"_blank\" rel=\"noopener\">Apple App Store<\/a><\/span> or <span style=\"color: #f58025;\"><a style=\"color: #f58025;\" href=\"https:\/\/play.google.com\/store\/apps\/details?id=com.neb.catalogar&amp;amp;hl=en_US&amp;amp;gl=US\" target=\"_blank\" rel=\"noopener\">Google Play<\/a><\/span> and scan the icons to find videos, tutorials and immersive experiences![\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/3&#8243;][vc_single_image image=&#8221;45168&#8243; img_size=&#8221;full&#8221;][\/vc_column][\/vc_row][vc_row bwd_tbc4vd_show_weekday=&#8221;&#8221;][vc_column][vc_column_text]<\/p>\n<h2><span style=\"color: #f58025;\">Product Selection<\/span><\/h2>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/interactive-tools\/competitor-cross-reference-tool\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-2017 alignleft\" src=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2019\/07\/Competitor_Cross-Reference.png\" alt=\"Competitor_Cross-Reference\" width=\"62\" height=\"64\" \/><\/a>[\/vc_column_text][vc_column_text]<a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/interactive-tools\/competitor-cross-reference-tool\" target=\"_blank\" rel=\"noopener noreferrer\">Competitor Cross-Reference Tools <\/a><\/p>\n<p>Use this tool to select another company&#8217;s product and find out which NEB product is compatible. Choose either the competitor\u2019s product name or catalog number from the available selections, and this tool will identify the recommended NEB product. [\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<a href=\"http:\/\/nebcloner.neb.com\/\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-2006 alignleft\" src=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/04\/icon_nebcloner_tool_150px.png\" alt=\"icon_nebcloner_tool_150px\" width=\"64\" height=\"64\" \/><\/a>[\/vc_column_text][vc_column_text]<a href=\"http:\/\/nebcloner.neb.com\/\" target=\"_blank\" rel=\"noopener noreferrer\">NEBCloner<\/a><\/p>\n<p>Use this tool to find the right products and protocols for each step (digestion, end modification, ligation and transformation) of your next traditional cloning experiment. Also, find other relevant tools and resources to enable protocol optimization.[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<a href=\"http:\/\/nebnextselector.neb.com\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-6991 alignleft\" src=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/09\/NEBNext_WebTool_62x64.png\" alt=\"NEBNext_WebTool_62x64\" width=\"62\" height=\"64\" \/><\/a>[\/vc_column_text][vc_column_text]<a href=\"http:\/\/nebnextselector.neb.com\" target=\"_blank\" rel=\"noopener noreferrer\">NEBNext Selector<\/a><\/p>\n<p>NEBNext Selector is a guide for selecting appropriate products for NextGen sequencing workflows.[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/interactive-tools\/pcr-selection-tool\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-2011 alignleft\" src=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/04\/NEB_PCR_Selector150.png\" alt=\"NEB_PCR_Selector150\" width=\"63\" height=\"64\" \/><\/a>[\/vc_column_text][vc_column_text]<a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/interactive-tools\/pcr-selection-tool\" target=\"_blank\" rel=\"noopener noreferrer\">PCR Selection Tool<\/a><\/p>\n<p>Use this tool to help select the right DNA polymerase for your PCR setup. Whether your amplicon is long, complex, GC-rich or present in a single copy, the PCR selection tool will identify the perfect DNA polymerase for your reaction.[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<\/p>\n<p style=\"text-align: center;\"><a href=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2020\/06\/Exo_Selector.jpg\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-22697 alignleft\" src=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2020\/06\/Exo_Selector.jpg\" alt=\"\" width=\"62\" height=\"62\" \/><\/a><\/p>\n<p>[\/vc_column_text][vc_column_text]<a href=\"https:\/\/exoselector.neb.com\/#!\/\" target=\"_blank\" rel=\"noopener noreferrer\">Exo Selector<\/a><\/p>\n<p>Use this tool to simplify the process of selecting the appropriate exonucleases for use in your nucleic acid digestion workflows. The tool guides you to product recommendations based on your answers to a few simple questions. [\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<img loading=\"lazy\" decoding=\"async\" class=\"wp-image-14067 alignleft\" src=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2018\/01\/PCRFidelityEstimator_WebTool_NEB_noName.png\" alt=\"\" width=\"64\" height=\"64\" \/>[\/vc_column_text][vc_column_text]<a href=\"http:\/\/pcrfidelityestimator.neb.com\/\" target=\"_blank\" rel=\"noopener noreferrer\">PCR Fidelity Estimator<\/a><\/p>\n<p>Estimate the percentage of correct DNA copies (those without base substitution errors) per cycle of PCR for selected DNA polymerases.[\/vc_column_text][\/vc_column][\/vc_row][vc_row bwd_tbc4vd_show_weekday=&#8221;&#8221; css=&#8221;.vc_custom_1627302126117{margin-top: 50px !important;}&#8221;][vc_column][vc_column_text]<\/p>\n<h2><span style=\"color: #f58025;\">Restriction Enzyme Tools<\/span><\/h2>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/interactive-tools\/double-digest-finder\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-2008 alignleft\" src=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/04\/NEB_DoubleDigestFinder150.png\" alt=\"NEB_DoubleDigestFinder150\" width=\"62\" height=\"64\" \/><\/a>[\/vc_column_text][vc_column_text]<a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/interactive-tools\/double-digest-finder\" target=\"_blank\" rel=\"noopener noreferrer\">Double Digest Finder<\/a><\/p>\n<p>Use this tool to guide your reaction buffer selection when setting up double-digests, a common timesaving procedure. Choosing the right buffers will help you to avoid star activity and loss of product.[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/interactive-tools\/enzyme-finder\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-2009 alignleft\" src=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/04\/NEB_Enzymfinder150.png\" alt=\"NEB_Enzymfinder150\" width=\"62\" height=\"64\" \/><\/a>[\/vc_column_text][vc_column_text]<a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/interactive-tools\/enzyme-finder\" target=\"_blank\" rel=\"noopener noreferrer\">Enzyme Finder<\/a><\/p>\n<p>Use this tool to select <a href=\"https:\/\/www.neb-online.de\/en\/cloning-synthetic-biology\/restriktionsenzyme\/\">restriction enzymes<\/a> by name, sequence, overhang or type. Enter your sequence using single letter code nomenclature, and Enzyme Finder will identify the right enzyme for the job.[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<a href=\"http:\/\/nc2.neb.com\/NEBcutter2\/\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-2007 alignleft\" src=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/04\/NEB_Cutter150.png\" alt=\"NEB_Cutter150\" width=\"62\" height=\"64\" \/><\/a>[\/vc_column_text][vc_column_text]<a href=\"https:\/\/nc3.neb.com\/NEBcutter\/\" target=\"_blank\" rel=\"noopener noreferrer\">NEBcutter V3.0<\/a><\/p>\n<p>Use this tool to identify the restriction sites within your DNA sequence. Choose between Type II and commercially available Type III <a href=\"https:\/\/www.neb-online.de\/en\/cloning-synthetic-biology\/restriktionsenzyme\/\">restriction enzymes<\/a> to digest your DNA. NEBcutter\u00ae V3.0 will indicate cut frequency and methylation state sensitivity.[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243;][\/vc_column][\/vc_row][vc_row bwd_tbc4vd_show_weekday=&#8221;&#8221; css=&#8221;.vc_custom_1627302126117{margin-top: 50px !important;}&#8221;][vc_column][vc_column_text]<\/p>\n<h2><span style=\"color: #f58025;\">Primer Design Tool<\/span><\/h2>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<a href=\"http:\/\/nebuilder.neb.com\/\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-2018 alignleft\" src=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/04\/NEBuilder150.png\" alt=\"NEBuilder\" width=\"63\" height=\"64\" \/><\/a>[\/vc_column_text][vc_column_text]<a href=\"http:\/\/nebuilder.neb.com\/\" target=\"_blank\" rel=\"noopener noreferrer\">NEBuilder\u00a0Assembly Tool<\/a><\/p>\n<p>NEBuilder\u00ae Assembly Tool can be used to design primers for your <a href=\"https:\/\/www.neb-online.de\/en\/cloning-synthetic-biology\/dna-assembly\/\">NEBuilder or Gibson Assembly <\/a>reaction, based on the entered fragment sequences and the polymerase being used for amplification.[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<a href=\"https:\/\/goldengate.neb.com\/#!\/\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-2053 alignleft\" src=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/04\/NEBGoldenGate_WebTool_NEB.png\" alt=\"Golden Gate Assembly\" width=\"62\" height=\"64\" \/><\/a>[\/vc_column_text][vc_column_text]<a href=\"https:\/\/goldengate.neb.com\/#!\/\" target=\"_blank\" rel=\"noopener noreferrer\">NEBridge Golden Gate Tool<\/a><\/p>\n<p>Use this tool to assist with in silico DNA construct design for <a href=\"https:\/\/www.neb-online.de\/en\/cloning-synthetic-biology\/dna-assembly\/\">Golden Gate DNA assembly<\/a>. It enables the accurate design of primers with appropriate type IIS restriction sites and overlaps, quick import of sequences in many formats and export of the final assembly, primers and settings.[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<a href=\"https:\/\/lamp.neb.com\/#!\/\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-2015 alignleft\" src=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2020\/12\/LAMPPrimerDesigner_WebTool_NEB.png \" alt=\"NEB_TmCalculator150\" width=\"62\" height=\"64\" \/><\/a>[\/vc_column_text][vc_column_text]<a class=\"tools-list__item-name\" href=\"https:\/\/lamp.neb.com\/\" data-is-punchout=\"False\">NEB LAMP Primer Design Tool <\/a><\/p>\n<p>NEB LAMP Primer Design Tool can be used to design primers for your Loop-mediated Isothermal Amplification. Fixed primers can be specified for the design of LAMP primers, and subsequent Loop primers are then designed based on LAMP primer selection.[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<a href=\"http:\/\/nebasechanger.neb.com\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-2016 alignleft\" src=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/04\/NEBaseChanger150.png\" alt=\"NEBaseChanger150\" width=\"62\" height=\"64\" \/><\/a>[\/vc_column_text][vc_column_text]<a href=\"http:\/\/nebasechanger.neb.com\" target=\"_blank\" rel=\"noopener noreferrer\">NEBaseChanger<\/a><\/p>\n<p>NEBaseChanger can be used to design primers specific to the mutagenesis experiment you are performing using the <a href=\"https:\/\/www.neb-online.de\/en\/pcr-and-dna-amplification\/q5-site-directed-mutagenesis-kit\/\">Q5\u00ae Site-Directed Mutagenesis Kit<\/a>. This tool will also calculate a recommended custom annealing temperature based on the sequence of the primers by taking into account any mismatches.[\/vc_column_text][\/vc_column][\/vc_row][vc_row bwd_tbc4vd_show_weekday=&#8221;&#8221; css=&#8221;.vc_custom_1627302126117{margin-top: 50px !important;}&#8221;][vc_column][vc_column_text]<\/p>\n<h2><span style=\"color: #f58025;\">Experimental Design<\/span><\/h2>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<\/p>\n<p style=\"text-align: center;\"><a href=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2020\/06\/GlycanAnalyzer_WebTool_NEB.png\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-22696 alignleft\" src=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2020\/06\/GlycanAnalyzer_WebTool_NEB-150x150.png\" alt=\"\" width=\"62\" height=\"64\" srcset=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2020\/06\/GlycanAnalyzer_WebTool_NEB-291x300.png 291w, https:\/\/www.neb-online.de\/wp-content\/uploads\/2020\/06\/GlycanAnalyzer_WebTool_NEB.png 397w\" sizes=\"auto, (max-width: 62px) 100vw, 62px\" \/><\/a><\/p>\n<p>[\/vc_column_text][vc_column_text]<a href=\"https:\/\/glycananalyzer.neb.com\/\" target=\"_blank\" rel=\"noopener noreferrer\">Glycan Analyzer<\/a><\/p>\n<p>Use this tool to interpret Ultra or High Pressure liquid chromatography (UPLC\/HPLC) N-glycan profiles following exoglycosidase digestions.[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<a href=\"http:\/\/nebcloner.neb.com\/\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-2006 alignleft\" src=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/04\/icon_nebcloner_tool_150px.png\" alt=\"icon_nebcloner_tool_150px\" width=\"64\" height=\"64\" \/><\/a>[\/vc_column_text][vc_column_text]<a href=\"http:\/\/nebcloner.neb.com\/\" target=\"_blank\" rel=\"noopener noreferrer\">NEBCloner<\/a><\/p>\n<p>Use this tool to find the right products and protocols for each step (digestion, end modification, ligation and transformation) of your next traditional cloning experiment. Also, find other relevant tools and resources to enable protocol optimization.[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/interactive-tools\/dna-sequences-and-maps-tool\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-2014 alignleft\" src=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/04\/NEB_SequenceMap150.png\" alt=\"NEB_SequenceMap150\" width=\"62\" height=\"64\" \/><\/a>[\/vc_column_text][vc_column_text]<a href=\"https:\/\/www.neb.com\/en\/tools-and-resources\/interactive-tools\/dna-sequences-and-maps-tool\" target=\"_blank\" rel=\"noopener noreferrer\">DNA Sequences &amp; Maps Tools<\/a><\/p>\n<p>Use this tool to find the nucleotide sequence files for commonly used molecular biology tools, including plasmid, viral and bacteriophage vectors.[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<a href=\"https:\/\/sgrna.neb.com\/#!\/sgrna\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-2014 alignleft\" src=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2020\/12\/sgRNADesigner_WebTool_NEB_noName.png\" alt=\"NEB_SequenceMap150\" width=\"62\" height=\"64\" \/><\/a>[\/vc_column_text][vc_column_text]<a class=\"pardotTrackClick tools-list__item-name\" href=\"https:\/\/sgrna.neb.com\/#!\/sgrna\" target=\"_blank\" rel=\"noopener\" data-is-punchout=\"False\">EnGen\u2122 sgRNA Template Oligo Designer <\/a><\/p>\n<p>EnGen\u2122 sgRNA Template Oligo Designer can be used to design target-specific DNA oligos for use with the EnGen\u2122 sgRNA Synthesis Kit, <em>S. pyogenes<\/em> (NEB #E3322).[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<a href=\"https:\/\/depletion-design.neb.com\/\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-2015 alignleft\" src=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2020\/12\/NEBNextCustomRNADepletion_WebTool_NEB_noName.png \" alt=\"NEB_TmCalculator150\" width=\"62\" height=\"64\" \/><\/a>[\/vc_column_text][vc_column_text]<a class=\"tools-list__item-name\" href=\"https:\/\/depletion-design.neb.com\/\" data-is-punchout=\"False\">NEBNext Custom RNA Depletion Design Tool <\/a><\/p>\n<p>This tool designs probes to be used with the NEBNext RNA Depletion Core Reagent Set or to supplement an existing NEBNext Depletion Kit for the depletion of unwanted RNA species.[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<img loading=\"lazy\" decoding=\"async\" class=\"wp-image-14067 alignleft\" src=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2018\/01\/PCRFidelityEstimator_WebTool_NEB_noName.png\" alt=\"\" width=\"64\" height=\"64\" \/>[\/vc_column_text][vc_column_text]<a href=\"http:\/\/pcrfidelityestimator.neb.com\/\" target=\"_blank\" rel=\"noopener noreferrer\">PCR Fidelity Estimator<\/a><\/p>\n<p>Estimate the percentage of correct DNA copies (those without base substitution errors) per cycle of PCR for selected DNA polymerases.[\/vc_column_text][\/vc_column][\/vc_row][vc_row bwd_tbc4vd_show_weekday=&#8221;&#8221; css=&#8221;.vc_custom_1627302126117{margin-top: 50px !important;}&#8221;][vc_column][vc_column_text]<\/p>\n<h2><span style=\"color: #f58025;\">Calculators<\/span><\/h2>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<a href=\"http:\/\/nebiocalculator.neb.com\/\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-2017 alignleft\" src=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/04\/NEBioCalculator150.png\" alt=\"NEBioCalculator150\" width=\"62\" height=\"64\" \/><\/a>[\/vc_column_text][vc_column_text]<a href=\"http:\/\/nebiocalculator.neb.com\/\" target=\"_blank\" rel=\"noopener noreferrer\">NEBioCalculator<\/a><\/p>\n<p>Use this tool for your scientific calculations and conversions for DNA and RNA. Options include conversion of mass to moles, ligation amounts, conversion of OD to concentration, dilution and molarity.[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<a href=\"http:\/\/ligasecalc.neb.com\/#!\/ligation\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-2014 alignleft\" src=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2019\/07\/Thermostable_Ligase-Reaction.png\" alt=\"Thermostable_Ligase-Reaction\" width=\"62\" height=\"64\" \/><\/a>[\/vc_column_text][vc_column_text]<a href=\"http:\/\/ligasecalc.neb.com\/#!\/ligation\" target=\"_blank\" rel=\"noopener noreferrer\">Thermostable Ligase Reaction Temperature Calculator <\/a><\/p>\n<p>This tool will estimate an optimal reaction temperature to minimize mismatch for thermostable ligation of two adjacent ssDNA probes annealed to a template. [\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<a href=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2020\/06\/ReadCoverageCalc_WebTool_NEB.png\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-22695 alignleft\" src=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2020\/06\/ReadCoverageCalc_WebTool_NEB-292x300.png\" alt=\"\" width=\"62\" height=\"64\" srcset=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2020\/06\/ReadCoverageCalc_WebTool_NEB-292x300.png 292w, https:\/\/www.neb-online.de\/wp-content\/uploads\/2020\/06\/ReadCoverageCalc_WebTool_NEB-768x788.png 768w, https:\/\/www.neb-online.de\/wp-content\/uploads\/2020\/06\/ReadCoverageCalc_WebTool_NEB-998x1024.png 998w, https:\/\/www.neb-online.de\/wp-content\/uploads\/2020\/06\/ReadCoverageCalc_WebTool_NEB.png 1205w\" sizes=\"auto, (max-width: 62px) 100vw, 62px\" \/><\/a>[\/vc_column_text][vc_column_text]<a href=\"http:\/\/readcoveragecalculator.neb.com\/#!\/\" target=\"_blank\" rel=\"noopener noreferrer\">Read Coverage Calculator<\/a><\/p>\n<p>This tool allows for easy calculation of values associated with read coverage in NGS protocols.[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<a href=\"http:\/\/tmcalculator.neb.com\/#!\/\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-2015 alignleft\" src=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/04\/NEB_TmCalculator150.png\" alt=\"NEB_TmCalculator150\" width=\"62\" height=\"64\" \/><\/a>[\/vc_column_text][vc_column_text]<a href=\"http:\/\/tmcalculator.neb.com\/#!\/\" target=\"_blank\" rel=\"noopener noreferrer\">Tm Calculator<\/a><\/p>\n<p>Use this tool when designing PCR reaction protocols to help determine the optimal annealing temperature for your amplicon. Simply input your DNA polymerase, primer concentration and your primer sequence and the Tm Calculator will guide you to successful reaction conditions.[\/vc_column_text][\/vc_column][\/vc_row][vc_row bwd_tbc4vd_show_weekday=&#8221;&#8221; css=&#8221;.vc_custom_1627302126117{margin-top: 50px !important;}&#8221;][vc_column][vc_column_text]<\/p>\n<h2><span style=\"color: #f58025;\">Databases <\/span><\/h2>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<a href=\"http:\/\/rebase.neb.com\/rebase\/\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-2013 alignleft\" src=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/04\/NEB_ReBase150.png\" alt=\"NEB_ReBase150\" width=\"62\" height=\"64\" \/><\/a>[\/vc_column_text][vc_column_text]<a href=\"http:\/\/rebase.neb.com\/rebase\/\">REBASE<\/a><\/p>\n<p>Use this tool as a guide to the ever-changing landscape of restriction enzymes. REBASE, the Restriction Enzyme DataBASE, is a dynamic, curated database of restriction enzymes and related proteins.[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<a href=\"https:\/\/polbase.neb.com\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-2012 alignleft\" src=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/04\/NEB_PolBase150.png\" alt=\"Icon Golden Gate Assembly Tool\" width=\"62\" height=\"64\" \/><\/a>[\/vc_column_text][vc_column_text]<a href=\"https:\/\/polbase.neb.com\" target=\"_blank\" rel=\"noopener noreferrer\">PolBase<\/a><\/p>\n<p>Polbase is a repository of biochemical, genetic, and structural information about DNA Polymerases.[\/vc_column_text][\/vc_column][\/vc_row][vc_row bwd_tbc4vd_show_weekday=&#8221;&#8221; css=&#8221;.vc_custom_1627302126117{margin-top: 50px !important;}&#8221;][vc_column][vc_column_text]<\/p>\n<h2><span style=\"color: #f58025;\">Additional Tools<\/span><\/h2>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/2&#8243;][vc_column_text]<a href=\"https:\/\/www.neb.com\/en\/research\/nebeta-tools\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-2013 alignleft\" src=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2021\/07\/NEBetaTools_NEB_noName.png\" alt=\"NEB_ReBase150\" width=\"62\" height=\"64\" \/><\/a>[\/vc_column_text][vc_column_text]<a href=\"https:\/\/www.neb.com\/en\/research\/nebeta-tools\">NEBeta\u2122 Tools<\/a><\/p>\n<p>View our selection of online tools currently in development. These tools are not yet optimized for design and usability \u2013 we are looking for feedback on functionality and utility to improve them for future use.[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243;][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row disable_element=&#8221;yes&#8221;][vc_column el_class=&#8221;tools-slider&#8221;][vc_images_carousel images=&#8221;6991,2007,2008,2015,2017,2018,2006,2013,2014,2016,2009,2053,2011,2012&#8243; img_size=&#8221;full&#8221; onclick=&#8221;custom_link&#8221; custom_links_target=&#8221;_blank&#8221; slides_per_view=&#8221;8&#8243; custom_links=&#8221;http:\/\/nebnextselector.neb.com,http:\/\/nc2.neb.com\/NEBcutter2\/,https:\/\/www.neb.com\/tools-and-resources\/interactive-tools\/double-digest-finder,http:\/\/tmcalculator.neb.com\/,http:\/\/nebiocalculator.neb.com\/,http:\/\/nebuilder.neb.com\/,http:\/\/nebcloner.neb.com\/,http:\/\/rebase.neb.com\/rebase\/,https:\/\/www.neb.com\/en\/tools-and-resources\/interactive-tools\/dna-sequences-and-maps-tool,http:\/\/nebasechanger.neb.com\/,https:\/\/www.neb.com\/tools-and-resources\/interactive-tools\/enzyme-finder,https:\/\/goldengate.neb.com\/editor,https:\/\/www.neb.com\/tools-and-resources\/interactive-tools\/pcr-selection-tool,http:\/\/polbase.neb.com\/&#8221; el_class=&#8221;tools-slider-8items&#8221;][vc_images_carousel images=&#8221;6991,2007,2008,2015,2017,2018,2006,2013,2014,2016,2009,2053,2011,2012&#8243; img_size=&#8221;full&#8221; onclick=&#8221;custom_link&#8221; custom_links_target=&#8221;_blank&#8221; slides_per_view=&#8221;4&#8243; custom_links=&#8221;http:\/\/nebnextselector.neb.com,http:\/\/nc2.neb.com\/NEBcutter2\/,https:\/\/www.neb.com\/tools-and-resources\/interactive-tools\/double-digest-finder,http:\/\/tmcalculator.neb.com\/,http:\/\/nebiocalculator.neb.com\/,http:\/\/nebuilder.neb.com\/,http:\/\/nebcloner.neb.com\/,http:\/\/rebase.neb.com\/rebase\/,https:\/\/www.neb.com\/en\/tools-and-resources\/interactive-tools\/dna-sequences-and-maps-tool,http:\/\/nebasechanger.neb.com\/,https:\/\/www.neb.com\/tools-and-resources\/interactive-tools\/enzyme-finder,https:\/\/goldengate.neb.com\/editor,https:\/\/www.neb.com\/tools-and-resources\/interactive-tools\/pcr-selection-tool,http:\/\/polbase.neb.com\/&#8221; el_class=&#8221;tools-slider-4items&#8221;][\/vc_column][\/vc_row][vc_row bwd_tbc4vd_show_weekday=&#8221;&#8221; css=&#8221;.vc_custom_1689680889623{margin-right: -1px !important;margin-left: -1px !important;border-top-width: 2px !important;border-right-width: 2px !important;border-bottom-width: 2px !important;border-left-width: 2px !important;padding-top: 10px !important;padding-right: 10px !important;padding-bottom: 10px !important;padding-left: 10px !important;border-left-color: #e0e0e0 !important;border-left-style: solid !important;border-right-color: #e0e0e0 !important;border-right-style: solid !important;border-top-color: #e0e0e0 !important;border-top-style: solid !important;border-bottom-color: #e0e0e0 !important;border-bottom-style: solid !important;border-radius:&#8230;  <a class=\"excerpt-read-more\" href=\"https:\/\/www.neb-online.de\/en\/technical-resources-neb\/neb-tools\/\" title=\"Read NEB Interactive Tools\">Read more &raquo;<\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"parent":3382,"menu_order":0,"comment_status":"open","ping_status":"closed","template":"page-sidebar.php","meta":{"footnotes":""},"class_list":["post-6407","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.1.1 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>NEB Interactive Tools - New England Biolabs GmbH<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.neb-online.de\/en\/technical-resources-neb\/neb-tools\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"NEB Interactive Tools - New England Biolabs GmbH\" \/>\n<meta property=\"og:description\" content=\"[vc_row disable_element=&#8221;yes&#8221;][vc_column el_class=&#8221;tools-slider&#8221;][vc_images_carousel images=&#8221;6991,2007,2008,2015,2017,2018,2006,2013,2014,2016,2009,2053,2011,2012&#8243; 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