{"id":9093,"date":"2016-05-02T10:48:09","date_gmt":"2016-05-02T08:48:09","guid":{"rendered":"http:\/\/www.neb-online.de\/en\/neb\/cloning-synthetic-biology\/dna-assembly\/nebuilder-dna-assembly\/"},"modified":"2023-12-15T11:49:51","modified_gmt":"2023-12-15T10:49:51","slug":"nebuilder-dna-assembly","status":"publish","type":"page","link":"https:\/\/www.neb-online.de\/en\/cloning-synthetic-biology\/dna-assembly\/nebuilder-dna-assembly\/","title":{"rendered":"NEBuilder HiFi DNA Assembly"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column][vc_basic_grid post_type=&#8221;post&#8221; max_items=&#8221;3&#8243; element_width=&#8221;12&#8243; item=&#8221;4123&#8243; grid_id=&#8221;vc_gid:1702637241710-b378e2a2-bfbf-2&#8243; taxonomies=&#8221;69&#8243;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]NEBuilder HiFi DNA Assembly enables virtually error-free joining of DNA fragments, even those with 5\u00b4 and 3\u00b4 restriction enzyme mismatches.<\/p>\n<p>This flexible kit enables simple and fast Seamless Cloning utilizing a new proprietary high-fidelity polymerase.<\/p>\n<p>Find out why NEBuilder HiFi is the next generation of DNA assembly and cloning.[\/vc_column_text][\/vc_column][\/vc_row][vc_row bwd_tbc4vd_show_weekday=&#8221;&#8221; css=&#8221;.vc_custom_1588949262284{border-top-width: 1px !important;border-right-width: 1px !important;border-bottom-width: 1px !important;border-left-width: 1px !important;border-left-color: #e0e0e0 !important;border-left-style: solid !important;border-right-color: #e0e0e0 !important;border-right-style: solid !important;border-top-color: #e0e0e0 !important;border-top-style: solid !important;border-bottom-color: #e0e0e0 !important;border-bottom-style: solid !important;border-radius: 1px !important;}&#8221;][vc_column]<div id=\"shorten-9155\" class=\"shorten\" style=\"max-height:300px;\">[vc_column_text]<style>.wForm form{text-align: left;}<\/style><!-- FORM: HEAD SECTION -->\n    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are required<\/div><\/div>\n<div class=\"htmlSection\" id=\"tfa_111\"><div class=\"htmlContent\" id=\"tfa_111-HTML\"><span style=\"font-size: 12.8px;\"><i>This offer is limited to new customers and to 1 sample per working group. Samples are available as long as supplies last.<\/i><\/span><\/div><\/div>\n<div class=\"oneField field-container-D    \" id=\"tfa_113-D\" role=\"group\" aria-labelledby=\"tfa_113-L\" data-tfa-labelledby=\"-L tfa_113-L\">\n<label id=\"tfa_113-L\" class=\"label preField \" data-tfa-check-label-for=\"tfa_113\">Choose your sample:<\/label><br><div class=\"inputWrapper\"><span id=\"tfa_113\" class=\"choices vertical \"><span class=\"oneChoice\"><input type=\"checkbox\" value=\"tfa_114\" class=\"calc-SAMPLE calcval-E2621G\\u0020NEBuilder\\u0020HiFi\\u0020DNA\\u0020Assembly\\u0020Master\\u0020Mix\\u0020Sample\\n\" id=\"tfa_114\" name=\"tfa_114\" aria-labelledby=\"tfa_114-L\" data-tfa-labelledby=\"tfa_113-L tfa_114-L\" data-tfa-parent-id=\"tfa_113\"><label class=\"label postField\" id=\"tfa_114-L\" for=\"tfa_114\"><span class=\"input-checkbox-faux\"><\/span>NEBuilder HiFi DNA Assembly Master Mix<\/label><\/span><span class=\"oneChoice\"><input type=\"checkbox\" value=\"tfa_115\" 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img_size=&#8221;full&#8221; img_link_large=&#8221;yes&#8221;][vc_single_image image=&#8221;9050&#8243; img_size=&#8221;full&#8221; img_link_large=&#8221;yes&#8221;][\/vc_column_inner][vc_column_inner width=&#8221;1\/2&#8243; css=&#8221;.vc_custom_1588949162374{margin-bottom: 10px !important;border-top-width: 2px !important;border-bottom-width: 2px !important;padding-top: 20px !important;padding-bottom: 20px !important;background-color: #f4f1e7 !important;border-top-color: #eb7e2d !important;border-top-style: solid !important;border-bottom-color: #eb7e2d !important;border-bottom-style: solid !important;}&#8221;][vc_column_text]<strong>Benefits over Gibson Assembly\u00ae Master Mix<\/strong><\/p>\n<ul>\n<li>Enjoy less screening\/re-sequencing of constructs, with virtually error-free, high-fidelity assembly<\/li>\n<li>Join DNA fragments together more efficiently, even with larger fragments or low DNA inputs<\/li>\n<li>Use NEBuilder HiFi in successive rounds of assembly, because it removes 5\u00b4- and 3\u00b4- restriction enzyme mismatches<\/li>\n<li>Bridge two double-stranded fragments with a synthetic single-stranded DNA oligo for simple and fast construction (e.g. linker insertion or gRNA libraries)<\/li>\n<li>Switch from other systems easily, because NEBuilder HiFi is compatible with Gibson Assembly-designed (and other) fragments<\/li>\n<li>Save time with simple and fast Seamless Cloning<\/li>\n<li>No licensing fee requirements from NEB for the NEBuilder products<\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=&#8221;black&#8221;][\/vc_column][\/vc_row][vc_row css=&#8221;.vc_custom_1461849499284{margin-right: -1px !important;margin-left: -1px !important;border-top-width: 2px !important;border-right-width: 2px !important;border-bottom-width: 2px !important;border-left-width: 2px !important;padding-top: 20px !important;padding-bottom: 20px !important;background-color: #f4f1e7 !important;border-left-color: #eb7e2d !important;border-left-style: solid !important;border-right-color: #eb7e2d !important;border-right-style: solid !important;border-top-color: #eb7e2d !important;border-top-style: solid !important;border-bottom-color: #eb7e2d !important;border-bottom-style: solid !important;}&#8221;][vc_column][vc_row_inner][vc_column_inner width=&#8221;1\/2&#8243; css=&#8221;.vc_custom_1461851120811{border-right-width: 1px !important;border-right-color: #444444 !important;border-right-style: solid !important;}&#8221;][vc_column_text css=&#8221;.vc_custom_1462180760997{margin-top: 20px !important;}&#8221;]<\/p>\n<h2><strong>NEBuilder\u00a0Assembly Tool<\/strong><\/h2>\n<p>Need help designing primers for DNA Assembly? Try <strong>NEBuilder Assembly Tool<\/strong>![\/vc_column_text][vc_column_text]<\/p>\n<p style=\"text-align: center;\"><span class=\"bildunterschrift\"><a href=\"http:\/\/nebuilder.neb.com\/\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-2018\" src=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/04\/NEBuilder150.png\" alt=\"NEBuilder\" width=\"63\" height=\"64\" \/><\/a>Please click the icon.<br \/>\n<\/span><\/p>\n<p>[\/vc_column_text][\/vc_column_inner][vc_column_inner width=&#8221;1\/2&#8243;][vc_column_text css=&#8221;.vc_custom_1462180839469{padding-top: 20px !important;}&#8221;]<\/p>\n<h2>NEBioCalculator<\/h2>\n<p>Use this tool for your scientific calculations and conversions for DNA and RNA[\/vc_column_text][vc_column_text]<\/p>\n<p style=\"text-align: center;\"><span class=\"bildunterschrift\"><a href=\"http:\/\/nebiocalculator.neb.com\/\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-2017\" src=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/04\/NEBioCalculator150.png\" alt=\"NEBioCalculator150\" width=\"62\" height=\"64\" \/><\/a>Please click the icon.<br \/>\n<\/span><\/p>\n<p>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=&#8221;black&#8221;][vc_column_text]<\/p>\n<h2>Comparison NEBuilder and Gibson Assembly<\/h2>\n<p>[\/vc_column_text][vc_row_inner][vc_column_inner width=&#8221;2\/3&#8243;][vc_column_text]<strong>NEBuilder HiFi DNA Assembly Master Mix offers improved efficiency and accuracy over Gibson Assembly with different numbers of fragments.<\/strong>[\/vc_column_text][vc_column_text]<span class=\"bildunterschrift\"><em>Reactions were set up in a 2- and 6- fragment assembly reaction according to recommended reaction conditions. <\/em><\/span>[\/vc_column_text][\/vc_column_inner][vc_column_inner width=&#8221;1\/3&#8243;][vc_single_image image=&#8221;1536&#8243; img_size=&#8221;full&#8221; alignment=&#8221;center&#8221; img_link_large=&#8221;yes&#8221;][\/vc_column_inner][\/vc_row_inner][vc_separator][vc_row_inner][vc_column_inner width=&#8221;1\/3&#8243;][vc_single_image image=&#8221;1542&#8243; img_size=&#8221;full&#8221; alignment=&#8221;center&#8221; img_link_large=&#8221;yes&#8221;][\/vc_column_inner][vc_column_inner width=&#8221;2\/3&#8243;][vc_column_text]<strong>NEBuilder HiFi DNA Assembly Master Mix offers improved efficiency and accuracy over Gibson Assembly, with lower amounts of DNA by increasing overlaps.<\/strong>[\/vc_column_text][vc_column_text]<span class=\"bildunterschrift\"><em>Reactions were set up in a 4- fragment assembly reaction according to recommended reaction conditions. Amount of DNA and size of overlap is shown. <\/em><\/span>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_separator][vc_row_inner][vc_column_inner width=&#8221;2\/3&#8243;][vc_column_text]<strong>NEBuilder HiFi DNA Master Mix offers improved fidelity over Gibson Assembly Master Mix<\/strong>[\/vc_column_text][vc_column_text]<span class=\"bildunterschrift\"><em>Fidelity of assembled products was compared between NEBuilder HiFi DNA Assembly Master Mix (NEB #E2621) and Gibson Assembly Master Mix (NEB #E2611). Experiments were performed using various fragment and vector sizes following suggested protocols. Experiments D and E did not yield any colonies with Gibson Assembly. Experiments H and J were performed with fragments containing 3\u00b4 restriction enzyme mismatches. Experiments B through G vary because sequences of fragments are different <\/em><\/span>[\/vc_column_text][\/vc_column_inner][vc_column_inner width=&#8221;1\/3&#8243;][vc_single_image image=&#8221;1546&#8243; img_size=&#8221;full&#8221; alignment=&#8221;center&#8221; img_link_large=&#8221;yes&#8221;][\/vc_column_inner][\/vc_row_inner][vc_separator color=&#8221;black&#8221;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text css=&#8221;.vc_custom_1461845865060{margin-top: 20px !important;padding-top: -20px !important;}&#8221;]<\/p>\n<h2>Videos<\/h2>\n<p>[\/vc_column_text][vc_row_inner][vc_column_inner width=&#8221;1\/3&#8243;][vc_video link=&#8221;https:\/\/youtu.be\/LwOYvWTm7kE&#8221;][\/vc_column_inner][vc_column_inner width=&#8221;1\/3&#8243;][vc_video link=&#8221;https:\/\/youtu.be\/8_-t5xtJ3y8&#8243;][\/vc_column_inner][vc_column_inner width=&#8221;1\/3&#8243;][vc_video link=&#8221;https:\/\/youtu.be\/4wv4ETxL5PE&#8221;][\/vc_column_inner][\/vc_row_inner][vc_row_inner css=&#8221;.vc_custom_1461845782523{margin-top: -20px !important;}&#8221;][vc_column_inner width=&#8221;1\/3&#8243;][vc_column_text]<strong>Introduction to NEBuilder HiFi DNA Assembly<\/strong>[\/vc_column_text][\/vc_column_inner][vc_column_inner width=&#8221;1\/3&#8243;][vc_column_text]<strong>Primer Design and Fragment Assembly Using NEBuilder HiFi DNA Assembly or Gibson Assembly<\/strong>[\/vc_column_text][\/vc_column_inner][vc_column_inner width=&#8221;1\/3&#8243;][vc_column_text]<strong>NEBuilder HiFi DNA Assembly: Bridging dsDNA with a ssDNA Oligo<\/strong>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row][vc_column][vc_separator color=&#8221;black&#8221;][\/vc_column][\/vc_row][vc_row css=&#8221;.vc_custom_1461915371370{margin-right: -1px !important;margin-left: -1px !important;border-top-width: 2px !important;border-right-width: 2px !important;border-bottom-width: 2px !important;border-left-width: 2px !important;padding-top: 20px !important;padding-bottom: 20px !important;background-color: #f4f1e7 !important;border-left-color: #eb7e2d !important;border-left-style: solid !important;border-right-color: #eb7e2d !important;border-right-style: solid !important;border-top-color: #eb7e2d !important;border-top-style: solid !important;border-bottom-color: #eb7e2d !important;border-bottom-style: solid !important;}&#8221;][vc_column][vc_row_inner][vc_column_inner][vc_column_text css=&#8221;.vc_custom_1466499826080{padding-top: 10px !important;}&#8221;]<\/p>\n<h2>3 new Application Notes<\/h2>\n<p>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner][vc_column_inner width=&#8221;1\/6&#8243;][vc_single_image image=&#8221;9031&#8243; img_size=&#8221;full&#8221;][\/vc_column_inner][vc_column_inner width=&#8221;5\/6&#8243;][vc_column_text]<strong>1.<\/strong> <a href=\"https:\/\/www.neb.com\/en\/-\/media\/nebus\/files\/application-notes\/construction-of-an-sgrna-cas9-expression-vector-via-single-stranded-dna-oligo-bridging-of-double-stranded-dna-fragments.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Construction of an sgRNA-Cas9 expression vector via single-stranded DNA oligo bridging of double-stranded DNA fragments E5520<\/a><\/p>\n<p><strong>2.<\/strong> <a href=\"https:\/\/www.neb.com\/~\/media\/Catalog\/All-Products\/BBFA4E32CB544B2D85B6C5110BE3A22B\/Application%20Notes\/Improved%20methods%20for%20site%20directed%20mutagenesis%20using%20NEBuilder%20HiFi%20DNA%20Assembly%20Master%20Mix.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Improved methods for site directed mutagenesis using NEBuilder HiFi DNA Assembly Master Mix<\/a><\/p>\n<p><strong>3.<\/strong> <a href=\"https:\/\/www.neb.com\/~\/media\/Catalog\/All-Products\/BBFA4E32CB544B2D85B6C5110BE3A22B\/Application%20Notes\/Improved%20method%20for%20assembly%20of%20linear%20yeast%20expression%20E5520.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Improved method for assembly of linear yeast expression E5520<\/a>[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row bwd_show_when_type=&#8221;before&#8221; bwd_show_when_date=&#8221;12\/31\/2023 23:00&#8243; bwd_tbc4vd_show_weekday=&#8221;&#8221;][vc_column][vc_separator color=&#8221;black&#8221;][vc_column_text]<\/p>\n<h2>Product table<\/h2>\n<table style=\"width: 760px;\" border=\"0\" cellpadding=\"5\">\n<tbody>\n<tr style=\"background-color: #f3f3f3; font-size: 16px;\">\n<th style=\"border-bottom: solid #eb7e2d;\" align=\"left\" width=\"400\" height=\"40px\">PRODUCT<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"88\">Prod. #<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"100\">SIZE<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" colspan=\"3\">PRICE<\/th>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">NEBuilder<sup>\u00ae<\/sup> HiFi DNA Assembly Cloning Kit<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E5520S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">10 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>204 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/E5520@\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/e5520\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"3\">NEBuilder<sup>\u00ae<\/sup> HiFi DNA Assembly Master Mix<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E2621S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">10 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>172 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/E2621@\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/e2621\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E2621L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">50 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>688 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/E2621@\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/e2621\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E2621X<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">250 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>2752 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/E2621@\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/e2621\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">NEBuilder<sup>\u00ae<\/sup> HiFi DNA Assembly Bundle for Large Fragments<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E2623S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">20 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: right; padding-right: 5px;\"><strong>530 \u20ac<\/strong><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/E2623@\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/e2623\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p style=\"text-align: right;\"><span class=\"bildunterschrift\">As of: 01.01.2023<\/span><\/p>\n<p>[\/vc_column_text][\/vc_column][\/vc_row][vc_row bwd_show_when_type=&#8221;after&#8221; bwd_show_when_date=&#8221;12\/31\/2023 23:00&#8243; bwd_tbc4vd_show_weekday=&#8221;&#8221;][vc_column][vc_separator color=&#8221;black&#8221;][vc_column_text]<\/p>\n<h2>Produkttabelle<\/h2>\n<table style=\"width: 760px;\" border=\"0\" cellpadding=\"5\">\n<tbody>\n<tr style=\"background-color: #f3f3f3; font-size: 16px;\">\n<th style=\"border-bottom: solid #eb7e2d;\" align=\"left\" width=\"400\" height=\"40\">PRODUCT<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"88\">Prod. #<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" width=\"100\">SIZE<\/th>\n<th style=\"border-bottom: solid #eb7e2d;\" colspan=\"2\">INFO<\/th>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">NEBuilder<sup>\u00ae<\/sup> HiFi DNA Assembly Cloning Kit<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E5520S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">10 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/E5520@\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/e5520\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" rowspan=\"3\">NEBuilder<sup>\u00ae<\/sup> HiFi DNA Assembly Master Mix<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E2621S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">10 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/E2621@\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/e2621\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E2621L<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">50 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/E2621@\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/e2621\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E2621X<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">250 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/E2621@\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/e2621\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"border-bottom: 1px solid #eb7e2d;\">NEBuilder<sup>\u00ae<\/sup> HiFi DNA Assembly Bundle for Large Fragments<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">E2623S<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d; text-align: center;\">20 rxns<\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb-online.de\/en\/art\/E2623@\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_shop.png\" alt=\"In den Shop wechseln\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<td style=\"border-bottom: 1px solid #eb7e2d;\" width=\"21px\"><a href=\"https:\/\/www.neb.com\/en\/e2623\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" style=\"float: right;\" src=\"https:\/\/www.neb-online.de\/enews\/enews_NEB_button_info.png\" alt=\"Produktinformationen\" width=\"20\" height=\"20\" \/><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p style=\"text-align: right;\"><span class=\"bildunterschrift\">As of 01.01.2024<\/span><\/p>\n<p>\u00a0[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row][vc_column][vc_basic_grid post_type=&#8221;post&#8221; max_items=&#8221;3&#8243; element_width=&#8221;12&#8243; item=&#8221;4123&#8243; grid_id=&#8221;vc_gid:1702637241710-b378e2a2-bfbf-2&#8243; taxonomies=&#8221;69&#8243;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]NEBuilder HiFi DNA Assembly enables virtually error-free joining of DNA fragments, even those with 5\u00b4 and 3\u00b4 restriction enzyme mismatches. This flexible kit enables simple and fast Seamless Cloning utilizing a new proprietary high-fidelity polymerase. Find out why NEBuilder HiFi is the next generation of DNA assembly and cloning.[\/vc_column_text][\/vc_column][\/vc_row][vc_row&#8230;  <a class=\"excerpt-read-more\" href=\"https:\/\/www.neb-online.de\/en\/cloning-synthetic-biology\/dna-assembly\/nebuilder-dna-assembly\/\" title=\"Read NEBuilder HiFi DNA Assembly\">Read more &raquo;<\/a><\/p>\n","protected":false},"author":18,"featured_media":40789,"parent":2066,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-sidebar.php","meta":{"footnotes":""},"class_list":["post-9093","page","type-page","status-publish","has-post-thumbnail","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.1.1 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>NEBuilder HiFi DNA Assembly - New England Biolabs GmbH<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.neb-online.de\/en\/cloning-synthetic-biology\/dna-assembly\/nebuilder-dna-assembly\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"NEBuilder HiFi DNA Assembly - New England Biolabs GmbH\" \/>\n<meta property=\"og:description\" content=\"[vc_row][vc_column][vc_basic_grid post_type=&#8221;post&#8221; max_items=&#8221;3&#8243; element_width=&#8221;12&#8243; item=&#8221;4123&#8243; grid_id=&#8221;vc_gid:1702637241710-b378e2a2-bfbf-2&#8243; taxonomies=&#8221;69&#8243;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]NEBuilder HiFi DNA Assembly enables virtually error-free joining of DNA fragments, even those with 5\u00b4 and 3\u00b4 restriction enzyme mismatches. This flexible kit enables simple and fast Seamless Cloning utilizing a new proprietary high-fidelity polymerase. Find out why NEBuilder HiFi is the next generation of DNA assembly and cloning.[\/vc_column_text][\/vc_column][\/vc_row][vc_row... Read more &raquo;\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.neb-online.de\/en\/cloning-synthetic-biology\/dna-assembly\/nebuilder-dna-assembly\/\" \/>\n<meta property=\"og:site_name\" content=\"New England Biolabs GmbH\" \/>\n<meta property=\"article:modified_time\" content=\"2023-12-15T10:49:51+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/04\/NEB_dnaassembly_Header_x760px.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"760\" \/>\n\t<meta property=\"og:image:height\" content=\"200\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"9 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.neb-online.de\/en\/cloning-synthetic-biology\/dna-assembly\/nebuilder-dna-assembly\/\",\"url\":\"https:\/\/www.neb-online.de\/en\/cloning-synthetic-biology\/dna-assembly\/nebuilder-dna-assembly\/\",\"name\":\"NEBuilder HiFi DNA Assembly - New England Biolabs GmbH\",\"isPartOf\":{\"@id\":\"https:\/\/www.neb-online.de\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/www.neb-online.de\/en\/cloning-synthetic-biology\/dna-assembly\/nebuilder-dna-assembly\/#primaryimage\"},\"image\":{\"@id\":\"https:\/\/www.neb-online.de\/en\/cloning-synthetic-biology\/dna-assembly\/nebuilder-dna-assembly\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/04\/NEB_dnaassembly_Header_x760px.jpg\",\"datePublished\":\"2016-05-02T08:48:09+00:00\",\"dateModified\":\"2023-12-15T10:49:51+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/www.neb-online.de\/en\/cloning-synthetic-biology\/dna-assembly\/nebuilder-dna-assembly\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/www.neb-online.de\/en\/cloning-synthetic-biology\/dna-assembly\/nebuilder-dna-assembly\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/www.neb-online.de\/en\/cloning-synthetic-biology\/dna-assembly\/nebuilder-dna-assembly\/#primaryimage\",\"url\":\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/04\/NEB_dnaassembly_Header_x760px.jpg\",\"contentUrl\":\"https:\/\/www.neb-online.de\/wp-content\/uploads\/2015\/04\/NEB_dnaassembly_Header_x760px.jpg\",\"width\":760,\"height\":200},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/www.neb-online.de\/en\/cloning-synthetic-biology\/dna-assembly\/nebuilder-dna-assembly\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/www.neb-online.de\/en\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Cloning \/ Synthetic Biology\",\"item\":\"https:\/\/www.neb-online.de\/en\/cloning-synthetic-biology\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"DNA Assembly\",\"item\":\"https:\/\/www.neb-online.de\/en\/cloning-synthetic-biology\/dna-assembly\/\"},{\"@type\":\"ListItem\",\"position\":4,\"name\":\"NEBuilder HiFi DNA Assembly\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/www.neb-online.de\/#website\",\"url\":\"https:\/\/www.neb-online.de\/\",\"name\":\"New England Biolabs GmbH\",\"description\":\"Enabling Technologies in the Life Sciences\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/www.neb-online.de\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"NEBuilder HiFi DNA Assembly - New England Biolabs GmbH","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.neb-online.de\/en\/cloning-synthetic-biology\/dna-assembly\/nebuilder-dna-assembly\/","og_locale":"en_US","og_type":"article","og_title":"NEBuilder HiFi DNA Assembly - New England Biolabs GmbH","og_description":"[vc_row][vc_column][vc_basic_grid post_type=&#8221;post&#8221; max_items=&#8221;3&#8243; element_width=&#8221;12&#8243; item=&#8221;4123&#8243; grid_id=&#8221;vc_gid:1702637241710-b378e2a2-bfbf-2&#8243; taxonomies=&#8221;69&#8243;][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]NEBuilder HiFi DNA Assembly enables virtually error-free joining of DNA fragments, even those with 5\u00b4 and 3\u00b4 restriction enzyme mismatches. 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