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Overview Illumina Roche/454 Solid Ion Torrent

 

 

NEBNext® DNA Library Preparation

 

Introducing NEBNext, a series of highly pure reagents that facilitate library preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction. Available in sets, master mixes and modules, these robust reagents undergo stringent quality controls and functional validation, ensuring maximum yield, convenience and value.



Advantages of NEBNext Library Prep Reagents:

  • Convenient formats – All of the required enzymes, buffers and nucleotides are included, and are also available in Master Mix format. Modules offer the ability to customize sample preparation.
     
  • Functional Validation – Each reagent set or module is functionally validated by preparation of a genomic DNA or ChIP-Seq DNA library that is sequenced using the appropriate sequencing platform (Illumina, 454 or SOLiD).
     
  • Stringent Quality Controls – Additional QCs ensure maximum quality and purity.
     
  • Value Pricing
     

Genomic Library Construction Workflow

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NEBNext offers a solution for each step of Genomic Library Construction Workflow

 

Currently available products:

PRODUCT NEB # SIZE COMPATIBLE
SEQUENCING
PLATFORM
NEBNext® DNA Library Prep Reagent Set for Illumina® E6000S/L 10/50 reactions Illumina®
NEBNext® DNA Library Prep Master Mix Set for Illumina® E6040S/L 12/60 reactions Illumina®
NEBNext® ChIP-Seq Library Prep Reagent Set for Illumina® E6200S/L 12/60 reactions Illumina®
NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina® E6240S/L 12/60 reactions Illumina®
NEBNext® Quick DNA Library Prep Reagent Set for 454™ E6080S/L 10/50 reactions 454™
NEBNext® Quick DNA Library Prep Master Mix Set for 454™ E6090S/L 10/50 reactions 454™
NEBNext® DNA Library Prep Reagent Set for 454™ E6020S/L 10/50 reactions 454™
NEBNext® DNA Library Prep Master Mix Set for 454™ E6070S/L 10/50 reactions 454™
NEBNext® DNA Library Prep Set for SOLiD™ E6060S/L 10/50 reactions SOLiD™
NEBNext® ChIP-Seq Library Prep Set for SOLiD™ E6260S/L 10/50 reactions SOLiD™
NEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent™ E6285S/L 10/50 reactions Ion Torrent™
NEBNext® Fast DNA Library Prep Set for Ion Torrent™ E6270S/L 10/50 reactions Ion Torrent™
NEBNext® End Repair Module E6050S/L 20/100 reactions Multiple
NEBNext® dA-Tailing Module E6053S/L 20/100 reactions Illumina®
NEBNext® Quick Ligation Module E6056S/L 20/100 reactions Multiple
NEBNext® Fill-in and ssDNA Isolation Module E6071S/L 20/100 reactions 454™
NEBNext® Singleplex Oligos for Illumina® E7350S/L 12/60 reactions Illumina®
NEBNext® Multiplex Oligos for Illumina® (Index Primers 1–12) E7335S/L 24/96 reactions Illumina®
NEBNext® dsDNA Fragmentase® M0348S/L 50/250 reactions Multiple
COMPANION PRODUCT
NEBNext® dsDNA Fragmentase® Reaction Buffer B0348S 6 x 1 ml Multiple
NEBNext® End Repair Reaction Buffer B6052S 2 ml Multiple
NEBNext® Quick Ligation Reaction Buffer B6058S 2 ml Multiple
NEBNext® dA-Tailing Reaction Buffer B6059S 2 ml Multiple



454™ is a trademark of Roche.
Illumina® is a registered trademark of Illumina, Inc.
SOLiD™ is a trademark of Life Technologies.
Ion Torrent™ is a trademark of Ion Torrent Systems Inc.