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Kompetente Zellen

Kompetente Zellen für eine Vielzahl von Anwendungen finden Sie bei New England Biolabs.

Für Klonierungsexperimente können Sie aus einer Reihe hoch-kompetenter E. coli Stämme wählen. Alle Stämme zeichnen sich durch Resistenz gegen den T1 Phagen und reduzierte Rekombinations-Aktivität aus.

Für die Protein-Expression bieten wir E. coli und Hefe (K. lactis) Stämme an, jeweils in verschiedenen Ausführungen, z.B. für verschiedene Arten der Expressionskontrolle.

Competent Cells – Cloning & Protein Expression

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Kompetente Zellen – Produktvergleich

Wenn Sie von einem anderen Stamm auf einen Stamm von New England Biolabs wechseln möchten, aber nicht sicher sind welcher unserer Stämme dem von Ihnen verwendeten entspricht, nutzen Sie unser Competitor Cross-Reference Tool.

Kompetente Zellen – Eigenschaften (E. coli)

Produkt NEB # Eigenschaften
Strains for Cloning and Subcloning
NEB® Stable Competent E. coli (High Efficiency) C3040H

C3040I

  • High quality plasmid preparations due to endA mutation
  • Rapid growth recA strain
  • T1 phage resistance (fhuA)
  • Value pricing
  • Free of animal products
NEB® Turbo Competent E. coli (High Efficiency) C2984H

C2984I

  • Fastest growth – colonies visible after 6.5 hours
  • Plasmid preparation after 4 hours
  • Tight control/expression of toxic genes (F´lacI8)
NEB® 5-alpha Competent E. coli (High Efficiency) C2987H

C2987I

C2987P

C2987R

C2987U

  • Versatile cloning strain
  • DH5α™ derivative
NEB® 5-alpha F‘ Iq Competent E. coli (High Efficiency) C2992H

C2992I

  • Toxic gene cloning
  • F´ strain with extremely high transformation efficiency
NEB® 10-beta Competent E. coli (High Efficiency) C3019H

C3019I

  • Large plasmid and BAC cloning
  • DH10B™ derivative
dam/dcm Competent E. coli C2925H

C2925I

  • Dam/Dcm methyltransferase free plasmid growth
Strains for Protein Expression
NEB® Express Competent E. coli (High Efficiency) C2523H

C2523I

  • Versatile non-T7 expression strain
  • Protease deficient
NEB® Express Iq Competent E. coli (High Efficiency) C3037I
  • Control of IPTG induced expression from Plac, Ptac,Ptrc and T5lac
  • Protease deficient
T7 Express Competent E. coli (High Efficiency) C2566H

C2566I

C2566A

C2566AA

  • Most popular T7 expression strain
  • Protease deficient
T7 Express lysY Competent E. coli (High Efficiency) C3010I

C3010A

C3010AA

  • T7 expression
  • Protease deficient
  • Better reduction of basal expression
T7 Express lysY/Iq Competent E. coli (High Efficiency) C3013I

C3013A

C3013AA

  • T7 expression
  • Protease deficient
  • Highest level of expression control
T7 Express Crystal Competent E. coli (High Efficiency) C3022I
  • T7 expression
  • Protease deficient
  • SeMet labeling for protein crystallography
SHuffle® Express Competent E. coli C3028J
  • Protease deficient/B strain
  • Enhanced capacity to correctly fold proteins with multiple disulfide bonds in the cytoplasm
SHuffle® T7 Express Competent E. coli C3029J
  • T7 expression
  • Protease deficient/B strain
  • Enhanced capacity to correctly fold proteins with multiple disulfide bonds in the cytoplasm
SHuffle® T7 Express lysY Competent E. coli C3030J
  • T7 expression
  • Protease deficient/B strain
  • Tightly controlled expression of toxic proteins
  • Enhanced capacity to correctly fold proteins with multiple disulfide bonds in the cytoplasm
SHuffle® T7 Competent E. coli C3026J
  • T7 expression/K12 strain
  • Enhanced capacity to correctly fold proteins with multiple disulfide bonds in the cytoplasm
BL21 Competent E. coli C2530H
  • Routine non-T7 expression
BL21(DE3) Competent E. coli C2527H

C2527I

  • Routine T7 expression
Lemo21(DE3) Competent E. coli C2528J
  • Tunable T7 expression for difficult targets
NiCo21(DE3) Competent E. coli C2529H
  • Improved purity of target proteins isolated by IMAC

Weiterführende Informationen finden Sie unter Technische Ressourcen oder auf neb.com