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Restriction Enzymes for Epigenetics

Many restriction enzymes are sensitive to the DNA methylation states. Cleavage can be blocked or impaired when a particular base in the recognition site is modified.

Scientists at NEB recently identified the MspJI family of restriction enzymes, which are dependent on methylation and hydroxymethylation for cleavage to occur. These enzymes excise ~ 32 base pair fragments containing a centrally located 5-hmC or 5-mC modified residue that can be extracted and sequenced. Due to the known position of this epigenetic modification, bisulfite conversion is not required prior to downstream analysis.

These EpiMark validated, methylation-dependent restriction enzymes expand the potential for mapping epigenetic modifications and simplify the study of DNA methylation. Additionally, they provide an opportunity to better understand the role of 5-hydroxymethylcytosine in the genome.

A variety of our existing restriction enzymes can also be used to study epigenetic modifications of DNA, such as, DpnI and DpnII that recognize the same sequence, but different methylation patterns. McrBC also only cleaves DNA that is methylated on one or both strands.

Advantages:
  • Specificity to epigenetically-relevant DNA modifications (5-mC and 5-hmC)
  • Easy-to-use protocols (enzymatic digestion followed by gel extraction)<
  • Less harsh than bisulfite conversion
  • Simplified data analysis
Methylation sensitive enzymes Gel

Simplify DNA methylation analysis with MspJI
MspJI recognizes methylated and hydroxymethylated DNA and cleaves out ~ 32 bp fragments for downstream sequencing analysis. Overnight digestion of 1 µg of genomic DNA from various sources with or without MspJI is shown. Note: Yeast DNA does not contain methylated DNA and thus, no 32-mer is detected.

Product table

PRODUCT Prod. # SIZE PRICE
MspJI, recombinant R0661S 200 units 111 € In den Shop wechseln Produktinformationen
R0661L 1.000 units 446 € In den Shop wechseln Produktinformationen
FspEI, recombinant R0662S 200 units 110 € In den Shop wechseln Produktinformationen
LpnPI, recombinant R0663S 200 units 112 € In den Shop wechseln Produktinformationen
DpnI, recombinant R0176S 1.000 units 66 € In den Shop wechseln Produktinformationen
R0176L 5.000 units 264 € In den Shop wechseln Produktinformationen
DpnII, recombinant R0543S 1.000 units 74 € In den Shop wechseln Produktinformationen
R0543L 5.000 units 297 € In den Shop wechseln Produktinformationen
DpnII, recombinant, conc. R0543T 1.000 units 74 € In den Shop wechseln Produktinformationen
R0543M 5.000 units 297 € In den Shop wechseln Produktinformationen
McrBC M0272S 500 units 72 € In den Shop wechseln Produktinformationen
M0272L 2.500 units 289 € In den Shop wechseln Produktinformationen
MspI, recombinant R0106S 5.000 units 65 € In den Shop wechseln Produktinformationen
R0106L 25.000 units 260 € In den Shop wechseln Produktinformationen
MspI, recombinant, conc. R0106T 5.000 units 65 € In den Shop wechseln Produktinformationen
R0106M 25.000 units 260 € In den Shop wechseln Produktinformationen
HpaII, recombinant R0171S 2.000 units 67 € In den Shop wechseln Produktinformationen
R0171L 10.000 units 269 € In den Shop wechseln Produktinformationen
HpaII, recombinant, conc. R0171M 10.000 units 269 € In den Shop wechseln Produktinformationen

As of: 01.01.2021

Further information can be found in our Technical Resources section or at neb.com. Information on trademarks can be found here.