New England Biolabs (NEB) offers a selection of endoglycosidases, exoglycosidases, and heparinases for glycobiology research. Many of these reagents are recombinant, and all undergo several quality control assays, enabling us to provide products with lower unit cost, high purity and reduced lot-to-lot variation. All of our glycosidases are tested for contaminants. Since p-nitrophenyl-glycosides are not hydrolyzed by some exoglycosidases, we use only fluorescently-labeled oligosaccharides to screen for contaminating glycosidases.
And, to simplify workflows and digestions with two or more enzymes, the number of glycosidase reaction buffers was recently decreased from 6 to 3.
Glycobiology Brochure
Remove-iT™ Endoglycosidase
Remove-iT PNGase F is an amidase which cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides from N-linked glycoproteins. Remove-iT PNGase F is tagged with a chitin binding domain (CBD) for easy removal from a reaction and is supplied glycerol free for optimal performance in HPLC and MS intensive methods.
Application Note
Remove-iT PNGase F: Effective Release and Recovery of Neutral and Sialylated N-glycans
Protein Deglycosylation Mix
The Protein Deglycosylation Mix kit contains all of the enzymes, reagents, and controls needed to remove almost all N-linked and simple O-linked glycans as well as some complex O-linked glycans. This kit contains enzyme sufficient for 20 reactions or the cleavage of as much as 2 mg of glycoprotein.
N– and O-Glycosylation
Stand der angegebenen Listenpreise: Dezember 2014. Die aktuellen Listenpreise können abweichen, bitte besuchen Sie für weitere Informationen unseren Online-Shop
| EXOGLYCOSIDASE ENZYMES | Art. # | Größe | Preis | ||
| α-N-Acetylgalactosaminidase | P0734 S/L | 3.000 units / 15.000 units | 124 € / 496 € |  |
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| β-N-Acetylglucosaminidase | P0732 S/L | 100 units / 500 units | 120 € / 480 € | |
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| β-N-Acetylhexosaminidasef | P0721 S/L | 500 units / 2.500 units | 62 € / 248 € | |
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| α1-2 Fucosidase | P0724 S/L | 1.000 units / 5.000 units | 120 €/ 480 € | |
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| α1-3,6 Galactosidase | P0731 S/L | 100 units / 500 units | 120 € / 480 € | |
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| β1-3 Galactosidase | P0726 S/L | 500 units / 2.500 units | 120 € / 480 € | |
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| β1-4 Galactosidase | P0730 S/L | 400 units / 2.000 units | 120 € / 480 € | |
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| α1-2,3 Mannosidase | P0729 S/L | 640 units / 3.200 units | 120 € / 480 € | |
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| α1-6 Mannosidase | P0727 S/L | 800 units / 4.000 units | 120 € / 480 € | |
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| Neuraminidase | P0720 S/L | 2.000 units / 10.000 units | 64 € / 256 € | |
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| α2,3 Neuraminidase | P0728 S/L | 2.5000 units / 12.5000 unts | 62 € / 248 € | |
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| Exoglycosidase Reaction Buffer Pack | B0719 S | 5,0 ml | 18 € | |
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| HEPARIN LYASES | Art. # | Größe | Preis | ||
| Bacteroides Heparinase I | P0735 S/L | 240 units / 600 units | 142 € / 284 € | |
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| Bacteroides Heparinase II | P0736 S/L | 80 units / 200 units | 215 € / 430 € | |
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| Bacteroides Heparinase III | P0737 S/L | 14 units / 35 units | 289 € / 578 € | |
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| MS STANDARDS | Art. # | Größe | Preis | ||
| Heparin Hexasaccharide MS Standard 6 | P0738 S | 40 nmol | 420 € | |
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| Heparin Hexasaccharide MS Standard 7 | P0739 S | 40 nmol | 420 € | |
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| GLYCOPROTEOMICS | Art. # | Größe | Preis | ||
| Trypsin-digested BSA MS Standards (CAM Modified) | P8108 S | 500 pmol | 70 € | |
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| Modified Trypsin (TPCK-treated) | P8101 S | 100 µg | 74 € | |
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| Endoproteinase GluC | P8100 S | 50 µg | 69 € | |
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Visit our shop to view our full range of Glycobiology products.
Further information can be found in our Technical Resources section or at neb.com. Information on trademarks can be found here.
