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Rapid PNGase F

Deglycosylation in less than 10 minutes!

PNGase F is the most effective enzymatic method for removing almost all N-linked oligosaccharides from glycoproteins. PNGase F digestion deaminates the asparagine residue to aspartic acid, and leaves the oligosaccharide intact, keeping it suitable for further analysis.

Rapid PNGase F is an improved reagent that allows the complete and rapid deglycosylation of antibodies and immunoglobulin fusion proteins, as well as other glycoproteins, in minutes – also available as non-reducing format. All N-glycans are released rapidly and without bias, and are ready to be prepared for downstream chromatography or mass spectrometry analysis. Rapid PNGase F creates an optimized workflow, reducing processing time without compromising sensitivity or reproducibility.

Advantages:
  • Complete deglycosylation of antibodies and immunoglobulin fusion proteins in minutes
  • Release of all N-glycans rapidly and without bias, ready for downstream chromatography or mass spectrometry analysis
  • Recombinant source
  • Optimal activity is ensured for 12 months
  • Purified to >99% homogeneity, as determined by SDS-PAGE

ESI-TOF analysis of an antibody before and after treatment with Rapid PNGase F.

ESI-TOF analysis of an antibody before and after treatment with Rapid PNGase F non-reducing format.

Product table

PRODUCT Prod. # SIZE PRICE
Rapid PNGase F P0710S 50 rxn 457 € In den Shop wechseln Produktinformationen
Rapid PNGase F (non-reducing format) P0711S 50 rxn 457 € In den Shop wechseln Produktinformationen

As of: 01.01.2021

Further information can be found in our Technical Resources section or at neb.com. Information on trademarks can be found here.