Deglycosylation in less than 10 minutes!
PNGase F is the most effective enzymatic method for removing almost all N-linked oligosaccharides from glycoproteins. PNGase F digestion deaminates the asparagine residue to aspartic acid, and leaves the oligosaccharide intact, keeping it suitable for further analysis.
Rapid PNGase F is an improved reagent that allows the complete and rapid deglycosylation of antibodies and immunoglobulin fusion proteins, as well as other glycoproteins, in minutes – also available as non-reducing format. All N-glycans are released rapidly and without bias, and are ready to be prepared for downstream chromatography or mass spectrometry analysis. Rapid PNGase F creates an optimized workflow, reducing processing time without compromising sensitivity or reproducibility.
- Complete deglycosylation of antibodies and immunoglobulin fusion proteins in minutes
- Release of all N-glycans rapidly and without bias, ready for downstream chromatography or mass spectrometry analysis
- Recombinant source
- Optimal activity is ensured for 12 months
- Purified to >99% homogeneity, as determined by SDS-PAGE
ESI-TOF analysis of an antibody before and after treatment with Rapid PNGase F.
ESI-TOF analysis of an antibody before and after treatment with Rapid PNGase F non-reducing format.