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Luna Universal qPCR & RT-qPCR

Fluorescence-based quantitative real-time PCR (qPCR) is the gold standard for the detection and quantification of nucleic acids due to its sensitivity and specificity. Luna products from NEB are optimized for qPCR or RT-qPCR, and are available for either intercalating dye or probe-based detection methods. All Luna products provide robust performance on diverse sample sources and target types.

Luna qPCR Selection Chart

Each Hot Start Taq-based Luna qPCR master mix has been formulated with a unique passive reference dye that is compatible across a wide variety of instrument platforms, including those that require a ROX reference signal. This means that no additional components are required to ensure machine compatibility. The mixes also contain dUTP, enabling carryover prevention when reactions are treated with NEB’s Antarctic Thermolabile UDG (NEB #M0372). A blue visible dye assists in tracking the reagents when pipetting into clear, multi-welled PCR plates. The reverse transcriptase, featured in the Luna RT-qPCR products is a novel, engineered WarmStart® enzyme developed for robust performance and increased thermostability. These features, combined with rapid, sensitive and precise real-time qPCR performance, make Luna the universal choice for all your qPCR and RT-qPCR experiments.

Experience best-in-class performance

  • All Luna products have undergone rigorous testing to optimize specificity, sensitivity, accuracy and reproducibility
  • Products perform consistently across a wide variety of sample sources
  • A comprehensive evaluation of commercially-available qPCR and RT-qPCR reagents demonstrates superior performance of Luna products
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Optimize your RT-qPCR with Luna WarmStart® Reverse Transcriptase

  • Employs novel, thermostable Reverse Transcriptases (RT) for fast protocols at elevated temperatures, exceptional robustness and sensitivity
  • One-Step Kits: the unique WarmStart RT paired with Hot Start Taq increases reaction specificity and robustness
  • Two-Step: the convenient LunaScript RT SuperMix Kit optimized for best in-class two-step protocols includes dNTPs, primers and RNase Inhibitor
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Make a simpler choice

  • One product per application simplifies selection
  • Convenient master mix formats and user-friendly protocols simplify reaction setup
  • Non-interfering, visible tracking dye helps to eliminate pipetting errors
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Overview of qPCR

Learn the basics of qPCR in this short animation.

“Dots in Boxes” Visualization of qPCR Data

Learn how we analyzed plates and plates of qPCR data, and how Luna stacks up to the competition.

More Video Tutorials on NEB’s YouTube Channel.

Find the right Luna product for your application

Click the respective product name for further information Chart

„Farbe als gute Pipettierhilfe, sehr schöne und ähnliche Kurvenverläufe bei unterschiedlichen Amplikons.“

NEB Kunde, TU München

„Gute Amplifikation.“

NEB Kunde, Uniklinik Freiburg

„Es scheint effizienter zu sein. Zudem vereinfacht der gefärbte Mastermix das Pipettieren.“

NEB Kundin, Max-Planck-Institut Dortmund

„Einfach in der Handhabung, passt gut in den bisherigen Arbeitsablauf bei gleichbleibenden oder sogar etwas besseren Ergebnissen“

NEB Kunde

„Die blaue Farbe, vermeidet Verwechslungen beim pipettieren.“

NEB Kundin, Universität Würzburg

„Hohe Amplifikationsrate und cDNA-Ausbeute.“

NEB Kundin, LAVES-Institut, Celle

„Leicht zu verstehen, tolles Handling.“

NEB Kunde

„Hat gleich beim ersten Lauf funktioniert.“

NEB Kunde aus Augsburg

„Sehr einfach und schnell.“

NEB Kunde

„Geringer Aufwand, tolle Ergebnisse.“

NEB Kundin, Universität Münster

„The results were very satisfying and that is what's important for me.“

NEB Kunde, Rudolf-Virchow-Zentrum, Würzburg

„Einfaches Setup, hohe Effizienz.“

NEB Kunde

Brochure:

Luna Universal qPCR & RT-qPCR

Lighting the way

Download

Feature Article:

Development of a high-throughput
data analysis method for quantitative
real-time PCR (qPCR)

Download PDF

Product table

PRODUCT Prod. # SIZE PRICE
Luna® Universal qPCR Master Mix M3003S 200 rxns (2x1ml) 105 € In den Shop wechseln Produktinformationen
M3003L 500 rxns (5x1ml) 237 € In den Shop wechseln Produktinformationen
M3003X 1000 rxns (10x1ml) 424 € In den Shop wechseln Produktinformationen
M3003E 2500 rxns (1x25ml) 948 € In den Shop wechseln Produktinformationen
Luna® Universal Probe qPCR Master Mix M3004S 200 rxns (2x1ml) 88 € In den Shop wechseln Produktinformationen
M3004L 500 rxns (5x1ml) 198 € In den Shop wechseln Produktinformationen
M3004X 1000 rxns (10x1ml) 364 € In den Shop wechseln Produktinformationen
M3004E 2500 rxns (1x25ml) 812 € In den Shop wechseln Produktinformationen
Luna® Universal One-Step RT-qPCR Kit E3005S 200 rxns 218 € In den Shop wechseln Produktinformationen
E3005L 500 rxns 498 € In den Shop wechseln Produktinformationen
E3005X 1000 rxns 869 € In den Shop wechseln Produktinformationen
E3005E 2500 rxns 2.924 € In den Shop wechseln Produktinformationen
Luna® Universal Probe One-Step RT-qPCR Kit E3006S 200 rxns 198 € In den Shop wechseln Produktinformationen
E3006L 500 rxns 448 € In den Shop wechseln Produktinformationen
E3006X 1000 rxns 788 € In den Shop wechseln Produktinformationen
E3006E 2500 rxns 1.738 € In den Shop wechseln Produktinformationen
LunaScript RT SuperMix Kit E3010S 25 rxns 125 € In den Shop wechseln Produktinformationen
E3010L 100 rxns 420 € In den Shop wechseln Produktinformationen

As of: 03.03.2019

Further information can be found in our Technical Resources section or at neb.com. Information on trademarks can be found here.